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The map label for this gene is ackA1 [H]

Identifier: 119946386

GI number: 119946386

Start: 3386156

End: 3387364

Strand: Direct

Name: ackA1 [H]

Synonym: Ping_2760

Alternate gene names: 119946386

Gene position: 3386156-3387364 (Clockwise)

Preceding gene: 119946384

Following gene: 119946392

Centisome position: 74.26

GC content: 43.51

Gene sequence:

>1209_bases
ATGAGCAACAAGCTAATTCTTGTACTTAACTGCGGCAGTTCTTCACTGAAGTTTGCCATCATCGACATGTCAACAGGTGA
AGAAAAACTATCCGGTCTTGCTGAATGTCTGCATTTGGATGATGCGCGCATGAAATGGAAATTAGAAGGCGCAAAAGGAA
GTGCAGATTTAGGTATTGGCGCCGCTCATGAAGAAGCTCTGAACTATATTGTCAATGAGATTCTGTCCAACCAACCTGAA
TTAAAAGAAAACCTCTTTGCAATTGGTCATCGTATCGTGCACGGAGGTGAAAAATTCACTCAATCTGTCGTGATAACAGA
TCAAGTCATCCAAGGCATTGAAGATTGCGTGTCACTCGCGCCTTTACATAATCCTGCTCACCTAGTCGGTATCCGTGCGG
CACAGCATGCTTTTGCCGGACTGCCAAATGTAGCCGTATTTGATACCGCTTTTCACCAGACTATGCCTGAAGAAGCGTTC
TTATATGCACTACCTTACAAACTTTACCGCGAAAAGGGTATCCGTCGTTACGGCATGCACGGTACTAGCCACCTTTACAT
TAGCCTTGAAGCTGCCGGCATATTAAACAAACCCGTTGAAGAGTTAAATATTATTAACTGTCACCTTGGTAACGGCGGCT
CAGTTTGTGCGATTAAAAACGGTAAGTCAGTTGATACTTCTATGGGAATGACTCCCTTAGAGGGTTTGGTAATGGGGACA
CGTTGTGGTGATATTGACCCGGCTGTTATCTTCCACCTGTATGATGAATTAGGTTATAGCATGGCAGAAATAAATACTAT
GCTGACCAAAGAGTCTGGATTATTAGGGTTAACTGAAAAAACATCTGATTGTCGTTTTGTTGAAGATAACTACGATACGG
ATCCGGCAGCTAAACGTGCCATGGATCTCTACTGCTACCGTCTGGCCAAATATATTGCCAGTTACACGGCAGCATTAGAC
GGACGATTAGACGCAGTGGTATTTACTGGTGGTATTGGTGAGAATTCAGCACCAATACGTGAGCTAACACTGGCGCGTTT
AAGTTTATTAGGTTTTAAAGTAAACCAGCAAGAGAACCTGGCAAACCGCTTTGGTAAATCATCGAATCAGGGCATTATTA
CTGAACCGGGTAGTACGGTCGCAATGGTTATTCAAACCAATGAAGAGTGGATTATTGCCCGTGATACATTAGCGTTAGTC
AGCGCTTAA

Upstream 100 bases:

>100_bases
TCTGGCATTTTCTAAAAATAGACAATATAACCTTTACAAAAATCCATCAAAATCTCAATCTGACCCACTGTTTTTACATA
ATAAATAGGAAATATAATAC

Downstream 100 bases:

>100_bases
TTGCCGCTATTTTATTGACAGTAAAGCTTGTAATAACACTAAGGATTACAAGCTTTTAATTTAATCCGGCACAGCCCGAT
AGGCACCGGATTATTATAAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSNKLILVLNCGSSSLKFAIIDMSTGEEKLSGLAECLHLDDARMKWKLEGAKGSADLGIGAAHEEALNYIVNEILSNQPE
LKENLFAIGHRIVHGGEKFTQSVVITDQVIQGIEDCVSLAPLHNPAHLVGIRAAQHAFAGLPNVAVFDTAFHQTMPEEAF
LYALPYKLYREKGIRRYGMHGTSHLYISLEAAGILNKPVEELNIINCHLGNGGSVCAIKNGKSVDTSMGMTPLEGLVMGT
RCGDIDPAVIFHLYDELGYSMAEINTMLTKESGLLGLTEKTSDCRFVEDNYDTDPAAKRAMDLYCYRLAKYIASYTAALD
GRLDAVVFTGGIGENSAPIRELTLARLSLLGFKVNQQENLANRFGKSSNQGIITEPGSTVAMVIQTNEEWIIARDTLALV
SA

Sequences:

>Translated_402_residues
MSNKLILVLNCGSSSLKFAIIDMSTGEEKLSGLAECLHLDDARMKWKLEGAKGSADLGIGAAHEEALNYIVNEILSNQPE
LKENLFAIGHRIVHGGEKFTQSVVITDQVIQGIEDCVSLAPLHNPAHLVGIRAAQHAFAGLPNVAVFDTAFHQTMPEEAF
LYALPYKLYREKGIRRYGMHGTSHLYISLEAAGILNKPVEELNIINCHLGNGGSVCAIKNGKSVDTSMGMTPLEGLVMGT
RCGDIDPAVIFHLYDELGYSMAEINTMLTKESGLLGLTEKTSDCRFVEDNYDTDPAAKRAMDLYCYRLAKYIASYTAALD
GRLDAVVFTGGIGENSAPIRELTLARLSLLGFKVNQQENLANRFGKSSNQGIITEPGSTVAMVIQTNEEWIIARDTLALV
SA
>Mature_401_residues
SNKLILVLNCGSSSLKFAIIDMSTGEEKLSGLAECLHLDDARMKWKLEGAKGSADLGIGAAHEEALNYIVNEILSNQPEL
KENLFAIGHRIVHGGEKFTQSVVITDQVIQGIEDCVSLAPLHNPAHLVGIRAAQHAFAGLPNVAVFDTAFHQTMPEEAFL
YALPYKLYREKGIRRYGMHGTSHLYISLEAAGILNKPVEELNIINCHLGNGGSVCAIKNGKSVDTSMGMTPLEGLVMGTR
CGDIDPAVIFHLYDELGYSMAEINTMLTKESGLLGLTEKTSDCRFVEDNYDTDPAAKRAMDLYCYRLAKYIASYTAALDG
RLDAVVFTGGIGENSAPIRELTLARLSLLGFKVNQQENLANRFGKSSNQGIITEPGSTVAMVIQTNEEWIIARDTLALVS
A

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=403, Percent_Identity=70.2233250620347, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=44.1919191919192, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43670; Mature: 43538

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKLILVLNCGSSSLKFAIIDMSTGEEKLSGLAECLHLDDARMKWKLEGAKGSADLGIG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
AAHEEALNYIVNEILSNQPELKENLFAIGHRIVHGGEKFTQSVVITDQVIQGIEDCVSLA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHC
PLHNPAHLVGIRAAQHAFAGLPNVAVFDTAFHQTMPEEAFLYALPYKLYREKGIRRYGMH
CCCCCHHEEEEHHHHHHHHCCCCCEEEHHHHHHCCCCHHEEEECCHHHHHHCCCHHHCCC
GTSHLYISLEAAGILNKPVEELNIINCHLGNGGSVCAIKNGKSVDTSMGMTPLEGLVMGT
CCEEEEEEEEECCCCCCCHHHCEEEEEEECCCCCEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPAVIFHLYDELGYSMAEINTMLTKESGLLGLTEKTSDCRFVEDNYDTDPAAKRA
CCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCHHHHHH
MDLYCYRLAKYIASYTAALDGRLDAVVFTGGIGENSAPIRELTLARLSLLGFKVNQQENL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCEECCCHHHH
ANRFGKSSNQGIITEPGSTVAMVIQTNEEWIIARDTLALVSA
HHHCCCCCCCCEEECCCCEEEEEEEECCCEEEEECCEEECCC
>Mature Secondary Structure 
SNKLILVLNCGSSSLKFAIIDMSTGEEKLSGLAECLHLDDARMKWKLEGAKGSADLGIG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
AAHEEALNYIVNEILSNQPELKENLFAIGHRIVHGGEKFTQSVVITDQVIQGIEDCVSLA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEHHHHHHHHHHHHHHC
PLHNPAHLVGIRAAQHAFAGLPNVAVFDTAFHQTMPEEAFLYALPYKLYREKGIRRYGMH
CCCCCHHEEEEHHHHHHHHCCCCCEEEHHHHHHCCCCHHEEEECCHHHHHHCCCHHHCCC
GTSHLYISLEAAGILNKPVEELNIINCHLGNGGSVCAIKNGKSVDTSMGMTPLEGLVMGT
CCEEEEEEEEECCCCCCCHHHCEEEEEEECCCCCEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPAVIFHLYDELGYSMAEINTMLTKESGLLGLTEKTSDCRFVEDNYDTDPAAKRA
CCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCHHHHHH
MDLYCYRLAKYIASYTAALDGRLDAVVFTGGIGENSAPIRELTLARLSLLGFKVNQQENL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCEECCCHHHH
ANRFGKSSNQGIITEPGSTVAMVIQTNEEWIIARDTLALVSA
HHHCCCCCCCCEEECCCCEEEEEEEECCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]