Click here to switch to the map view.

The map label for this gene is garR [C]

Identifier: 119946376

GI number: 119946376

Start: 3373800

End: 3374675

Strand: Reverse

Name: garR [C]

Synonym: Ping_2750

Alternate gene names: 119946376

Gene position: 3374675-3373800 (Counterclockwise)

Preceding gene: 119946378

Following gene: 119946372

Centisome position: 74.01

GC content: 48.86

Gene sequence:

>876_bases
ATGAAGGTTGCATTTATCGGCTTAGGCGTTATGGGTTACCCGATGGCAGGATTTTTAAAGCAGGCGGGTCATGATGTTTG
TGTCTATAACCGCACATTAGCCAAGGCACAGAAGTGGCAGCAACAATATCAGGGCAGCATAGGCCTGACTCCACGTGAAG
CCGCTCAAGACGCTGAAATTGTGTTTAGCTGTGTTGGCAATGACAATGATTTACGCGCGGTTGTTTTGGGTGAAGATGGC
ATGCTGGCAGGAATGAAGAGCGGCGCTATATTAGTTGATCATACAACGGCATCTGCCGATGTTGCTCGTGAACTGGCAGC
GCTCTGTGCTGAGCAAGGCAAAGGATTTCTCGACGCACCGGTATCCGGTGGTCAGGCGGGCGCAGAAAAGGGTATTTTAA
CCATTATGATTGGCGGTGACAGCGCGGTATTTGAGAAAGTGTGTCCGGTTATTGATGCCTATGCTCGCTGTGCTGAACGG
ATTGGTGATGTGGGCAGTGGTCAGCTGGCTAAAATGGTTAACCAGATCTGTATTGCCGGCGCGGTGCAAGGCTTGGCTGA
GGCGATTCATTTTGCCAAATGTGCCGATCTTGATGTCAATAAAGTCATTGATGTGATCTCCAAAGGCGCGGCGCAGTCGT
GGCAAATGGAGAATCGTCATCAGGCAATGATCGACAATAGTTATGAATTTGGTTTTGCGGTTGACTGGATGCGCAAAGAT
TTAGCGATTACGCTTAATGAAGCACGCCACAATAAGGCAAACTTACCAGTGACAGCAGTAGTCGATCAGTTTTATGGTGA
AGTGCAAGGCATGGGGGGAAGTCGCTGGGACACCGCAAGCTTACATGCCCGGTTAGAAAAATTTACCGATAAATAA

Upstream 100 bases:

>100_bases
CATCAATAAAAGGTTTGGATATTCGGCACTTTAGCCTTTTATATTTCCATAGCCGTTTATCATTTTTAATCACTCATTTA
TTCAAGAAAGGAGCATTATT

Downstream 100 bases:

>100_bases
TCTTTGGTCAGGCTCAGATTGACTAAAGCCAATCAAACAAAACGCTGAAAAGATTGACGTTGTACACTGTGGTTGTAGAA
TAAAATATAAGCGGTAAAGC

Product: 2-hydroxy-3-oxopropionate reductase GarR

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKVAFIGLGVMGYPMAGFLKQAGHDVCVYNRTLAKAQKWQQQYQGSIGLTPREAAQDAEIVFSCVGNDNDLRAVVLGEDG
MLAGMKSGAILVDHTTASADVARELAALCAEQGKGFLDAPVSGGQAGAEKGILTIMIGGDSAVFEKVCPVIDAYARCAER
IGDVGSGQLAKMVNQICIAGAVQGLAEAIHFAKCADLDVNKVIDVISKGAAQSWQMENRHQAMIDNSYEFGFAVDWMRKD
LAITLNEARHNKANLPVTAVVDQFYGEVQGMGGSRWDTASLHARLEKFTDK

Sequences:

>Translated_291_residues
MKVAFIGLGVMGYPMAGFLKQAGHDVCVYNRTLAKAQKWQQQYQGSIGLTPREAAQDAEIVFSCVGNDNDLRAVVLGEDG
MLAGMKSGAILVDHTTASADVARELAALCAEQGKGFLDAPVSGGQAGAEKGILTIMIGGDSAVFEKVCPVIDAYARCAER
IGDVGSGQLAKMVNQICIAGAVQGLAEAIHFAKCADLDVNKVIDVISKGAAQSWQMENRHQAMIDNSYEFGFAVDWMRKD
LAITLNEARHNKANLPVTAVVDQFYGEVQGMGGSRWDTASLHARLEKFTDK
>Mature_291_residues
MKVAFIGLGVMGYPMAGFLKQAGHDVCVYNRTLAKAQKWQQQYQGSIGLTPREAAQDAEIVFSCVGNDNDLRAVVLGEDG
MLAGMKSGAILVDHTTASADVARELAALCAEQGKGFLDAPVSGGQAGAEKGILTIMIGGDSAVFEKVCPVIDAYARCAER
IGDVGSGQLAKMVNQICIAGAVQGLAEAIHFAKCADLDVNKVIDVISKGAAQSWQMENRHQAMIDNSYEFGFAVDWMRKD
LAITLNEARHNKANLPVTAVVDQFYGEVQGMGGSRWDTASLHARLEKFTDK

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=29.8611111111111, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=28.0821917808219, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=34.4947735191638, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=31.25, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=28.3687943262411, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789092, Length=283, Percent_Identity=30.0353356890459, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=25.177304964539, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31075; Mature: 31075

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAFIGLGVMGYPMAGFLKQAGHDVCVYNRTLAKAQKWQQQYQGSIGLTPREAAQDAEI
CEEEEEECCHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHE
VFSCVGNDNDLRAVVLGEDGMLAGMKSGAILVDHTTASADVARELAALCAEQGKGFLDAP
EEEECCCCCCEEEEEECCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEECC
VSGGQAGAEKGILTIMIGGDSAVFEKVCPVIDAYARCAERIGDVGSGQLAKMVNQICIAG
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVQGLAEAIHFAKCADLDVNKVIDVISKGAAQSWQMENRHQAMIDNSYEFGFAVDWMRKD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHCCCCCCCEEHHHHHCC
LAITLNEARHNKANLPVTAVVDQFYGEVQGMGGSRWDTASLHARLEKFTDK
CEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVAFIGLGVMGYPMAGFLKQAGHDVCVYNRTLAKAQKWQQQYQGSIGLTPREAAQDAEI
CEEEEEECCHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHE
VFSCVGNDNDLRAVVLGEDGMLAGMKSGAILVDHTTASADVARELAALCAEQGKGFLDAP
EEEECCCCCCEEEEEECCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEECC
VSGGQAGAEKGILTIMIGGDSAVFEKVCPVIDAYARCAERIGDVGSGQLAKMVNQICIAG
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVQGLAEAIHFAKCADLDVNKVIDVISKGAAQSWQMENRHQAMIDNSYEFGFAVDWMRKD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHCCCCCCCEEHHHHHCC
LAITLNEARHNKANLPVTAVVDQFYGEVQGMGGSRWDTASLHARLEKFTDK
CEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA