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The map label for this gene is pleD [H]

Identifier: 119946361

GI number: 119946361

Start: 3352769

End: 3353620

Strand: Reverse

Name: pleD [H]

Synonym: Ping_2732

Alternate gene names: 119946361

Gene position: 3353620-3352769 (Counterclockwise)

Preceding gene: 119946363

Following gene: 119946359

Centisome position: 73.55

GC content: 43.31

Gene sequence:

>852_bases
TTGGGCTTAAATTTGTTATTACTGCTATCTAACAGTCTGGTGCTGGTGGGCATATTAATCCTTCTGGGATCTCTTCTTAC
GGTTAGGCGAATTCTCCGAAGTTTTCCTGAGGGGCGATCTCATAAGGGCTGGTTTACCATGGGCTGCCTGATTAGTCTGT
TTGTGCTTGGTTATCTGGCTTATGTCAATATTTTTTGGGAAAAACATACACAAGCAACGGATCTCATTGTGCCTGGTGTA
TTCTTTTTTGGCGCCTGTTTTGTATTGTTATCAACCTTCCTGTCTTTAAACACTGCGCTGGATATTATACGCATCTGCGA
ACTGGAAAAAGAGTCTTCCACTGACCCTCTGACCGGTGTATATAACCGCCGACATATGGAAAAATGCCTGCAGGAAGAGA
TAGCAAAAGCGCAGCGATACAAATTTGATCTCTCACTATTACTGATCGACTTGGACAATTTCAAACTGATAAACGATCAG
CACGGGCACCAAGTCGGCGATCAAGTGCTTACTGACATTAGCGCGTTAGTGCTTCAAGAGCTTCGCAGCTTCGATGTTAT
GGCGCGTTACGGGGGGGAGGAATTTCTTGTAATTGCACCCAATACGGGTCCTGGATATGCCCATATTCTGGCAGAACGAT
TACGCAAAAGAATTGAGATGCATCATTTTTTTGTGGGTTCCAAAGCACTTAAAATCAAACTAACCATCAGTATAGGGGTG
TCGAGTTACGGTGGTTCTATCACAACTCAAGAAAGCTTAGTTCTTCGAGCAGATAATAATCTTTATCTGGCAAAGGGACA
GGGGCGTAACAAGGTAATAGCAGACCAATCAAAATATCCTCTTCAAGTGTAA

Upstream 100 bases:

>100_bases
ATACCGTTTTTGAGGTTTTTCCAAAACAAAATGCAAAAAACAGCAGCCACTTAACGGCCAGTGAGCGCGGCGTTGGTTCT
TATTCAGTCATCCAAATAAA

Downstream 100 bases:

>100_bases
CCCTTTCTGGCAAAGTGCTAACGTTTTAATATTGGTAAAAATCCTGCAAATATAAAAACCGTTTTTATGCTTAAAAAATA
GCATTTCAAATTTATTTTTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGLNLLLLLSNSLVLVGILILLGSLLTVRRILRSFPEGRSHKGWFTMGCLISLFVLGYLAYVNIFWEKHTQATDLIVPGV
FFFGACFVLLSTFLSLNTALDIIRICELEKESSTDPLTGVYNRRHMEKCLQEEIAKAQRYKFDLSLLLIDLDNFKLINDQ
HGHQVGDQVLTDISALVLQELRSFDVMARYGGEEFLVIAPNTGPGYAHILAERLRKRIEMHHFFVGSKALKIKLTISIGV
SSYGGSITTQESLVLRADNNLYLAKGQGRNKVIADQSKYPLQV

Sequences:

>Translated_283_residues
MGLNLLLLLSNSLVLVGILILLGSLLTVRRILRSFPEGRSHKGWFTMGCLISLFVLGYLAYVNIFWEKHTQATDLIVPGV
FFFGACFVLLSTFLSLNTALDIIRICELEKESSTDPLTGVYNRRHMEKCLQEEIAKAQRYKFDLSLLLIDLDNFKLINDQ
HGHQVGDQVLTDISALVLQELRSFDVMARYGGEEFLVIAPNTGPGYAHILAERLRKRIEMHHFFVGSKALKIKLTISIGV
SSYGGSITTQESLVLRADNNLYLAKGQGRNKVIADQSKYPLQV
>Mature_282_residues
GLNLLLLLSNSLVLVGILILLGSLLTVRRILRSFPEGRSHKGWFTMGCLISLFVLGYLAYVNIFWEKHTQATDLIVPGVF
FFGACFVLLSTFLSLNTALDIIRICELEKESSTDPLTGVYNRRHMEKCLQEEIAKAQRYKFDLSLLLIDLDNFKLINDQH
GHQVGDQVLTDISALVLQELRSFDVMARYGGEEFLVIAPNTGPGYAHILAERLRKRIEMHHFFVGSKALKIKLTISIGVS
SYGGSITTQESLVLRADNNLYLAKGQGRNKVIADQSKYPLQV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=38.2716049382716, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=35.8288770053476, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=36.875, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.6470588235294, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=36.1963190184049, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=38.953488372093, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=35.625, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=36.6459627329193, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=32.3529411764706, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=30.3867403314917, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=134, Percent_Identity=32.089552238806, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787056, Length=176, Percent_Identity=32.3863636363636, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 31762; Mature: 31630

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNLLLLLSNSLVLVGILILLGSLLTVRRILRSFPEGRSHKGWFTMGCLISLFVLGYLA
CCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YVNIFWEKHTQATDLIVPGVFFFGACFVLLSTFLSLNTALDIIRICELEKESSTDPLTGV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
YNRRHMEKCLQEEIAKAQRYKFDLSLLLIDLDNFKLINDQHGHQVGDQVLTDISALVLQE
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHH
LRSFDVMARYGGEEFLVIAPNTGPGYAHILAERLRKRIEMHHFFVGSKALKIKLTISIGV
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCEEEEEEEEEEEEE
SSYGGSITTQESLVLRADNNLYLAKGQGRNKVIADQSKYPLQV
ECCCCCCEECCEEEEEECCCEEEEECCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
GLNLLLLLSNSLVLVGILILLGSLLTVRRILRSFPEGRSHKGWFTMGCLISLFVLGYLA
CCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YVNIFWEKHTQATDLIVPGVFFFGACFVLLSTFLSLNTALDIIRICELEKESSTDPLTGV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
YNRRHMEKCLQEEIAKAQRYKFDLSLLLIDLDNFKLINDQHGHQVGDQVLTDISALVLQE
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHH
LRSFDVMARYGGEEFLVIAPNTGPGYAHILAERLRKRIEMHHFFVGSKALKIKLTISIGV
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCEEEEEEEEEEEEE
SSYGGSITTQESLVLRADNNLYLAKGQGRNKVIADQSKYPLQV
ECCCCCCEECCEEEEEECCCEEEEECCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA