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The map label for this gene is gcvH

Identifier: 119946357

GI number: 119946357

Start: 3346329

End: 3346706

Strand: Direct

Name: gcvH

Synonym: Ping_2728

Alternate gene names: 119946357

Gene position: 3346329-3346706 (Clockwise)

Preceding gene: 119946354

Following gene: 119946358

Centisome position: 73.39

GC content: 41.01

Gene sequence:

>378_bases
ATGGGAAGCGCATTAATGTTTACTGATACACATGAGTGGATACTCGATAACGGTGATGGTAGCGTCACTATCGGTATTTC
AAACCAGGCTCAACAACTTTTAGGAGATGTTGTCTTTGTTGATATGCCAGAAGTGGGTGATAACACGCAAGCGGGGGAAA
CTTTCTCTTTAGTGGAATCAGTTAAAGCAGCCTCGGATCTCTATGCACCCGTAACAGGTGAAATTATTGAGATTAACGAA
GAACTTGAAGATAGCCCCGAATTAATTAATGAATCCCCCTATTCTGCAGGTTGGATTGTCAAAATCAAATTAGCTGATCG
CAATGAGCTTAATAATCTTATGTCCAGTGAAGATTATCTCGCGGCGAATGCAGAATAA

Upstream 100 bases:

>100_bases
TCTTATCGATTTAAATAAAACCACATTCATTATTGTCATTAGCTCTCGATGAGTCATTAGCAATAAATATTTCACACAAC
CAATTAGCTAAGGATTTAAT

Downstream 100 bases:

>100_bases
TCATTCATTCACAACCTATCTAATCAATTAGGCTGCTTCGAATGAAGCTGCCTTGCTTTTTTTCTGCTATCTCCCCTCTT
AATCAACATAAAACAGGGGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MGSALMFTDTHEWILDNGDGSVTIGISNQAQQLLGDVVFVDMPEVGDNTQAGETFSLVESVKAASDLYAPVTGEIIEINE
ELEDSPELINESPYSAGWIVKIKLADRNELNNLMSSEDYLAANAE

Sequences:

>Translated_125_residues
MGSALMFTDTHEWILDNGDGSVTIGISNQAQQLLGDVVFVDMPEVGDNTQAGETFSLVESVKAASDLYAPVTGEIIEINE
ELEDSPELINESPYSAGWIVKIKLADRNELNNLMSSEDYLAANAE
>Mature_124_residues
GSALMFTDTHEWILDNGDGSVTIGISNQAQQLLGDVVFVDMPEVGDNTQAGETFSLVESVKAASDLYAPVTGEIIEINEE
LEDSPELINESPYSAGWIVKIKLADRNELNNLMSSEDYLAANAE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=49.5934959349593, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=48.780487804878, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789271, Length=117, Percent_Identity=52.991452991453, Blast_Score=140, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=114, Percent_Identity=45.6140350877193, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=44.0677966101695, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=119, Percent_Identity=41.1764705882353, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=43.4782608695652, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PSYIN (A1SY73)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944037.1
- ProteinModelPortal:   A1SY73
- SMR:   A1SY73
- STRING:   A1SY73
- GeneID:   4626390
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2728
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   FTDSHEW
- PhylomeDB:   A1SY73
- ProtClustDB:   PRK01202
- BioCyc:   PING357804:PING_2728-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13539; Mature: 13408

Theoretical pI: Translated: 3.49; Mature: 3.49

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSALMFTDTHEWILDNGDGSVTIGISNQAQQLLGDVVFVDMPEVGDNTQAGETFSLVES
CCCEEEEECCCEEEEECCCCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHH
VKAASDLYAPVTGEIIEINEELEDSPELINESPYSAGWIVKIKLADRNELNNLMSSEDYL
HHHHHHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCCE
AANAE
EECCC
>Mature Secondary Structure 
GSALMFTDTHEWILDNGDGSVTIGISNQAQQLLGDVVFVDMPEVGDNTQAGETFSLVES
CCEEEEECCCEEEEECCCCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHH
VKAASDLYAPVTGEIIEINEELEDSPELINESPYSAGWIVKIKLADRNELNNLMSSEDYL
HHHHHHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCCE
AANAE
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA