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The map label for this gene is proB [H]

Identifier: 119946272

GI number: 119946272

Start: 3232755

End: 3233633

Strand: Reverse

Name: proB [H]

Synonym: Ping_2635

Alternate gene names: 119946272

Gene position: 3233633-3232755 (Counterclockwise)

Preceding gene: 119946274

Following gene: 119946271

Centisome position: 70.92

GC content: 40.27

Gene sequence:

>879_bases
ATGAATACAAATACTAATACTAATACAAAGCCAAAGACAAAAAATAATACAAAACGTGTAGTGATTAAAGTCGGCAGTGC
AGTATTGACAGATAATAATAACATTGCCAAGGAGCGCATGCTTAACTTGGTATCTATGATCTCTGGACTAAGAAAAACCT
ACGACGTCATTCTTGTGACTTCCGGTGCGGTCGCGGCAGGGTACTCGACTCTAAAGCTTGACAAAAGTAAACAGATTGGC
AAGAAGGCACTTGCAGCGGCAGGTCAGCCTATTTTAATGACGGCCTATAAAAAGAAATTTGATATCTATGATATCGACAC
TGCGCAGATCCTGCTTACAGAAGATGATTTTGACTCAAGAAAACGGACTAAGATGTTTCAGGAAATAATCGATGCTCATC
TGACACACGATATCCTGCCAATCGTCAATGAAAACGATATTACCTCAACACCAGAACAGCAGTTTGGCGACAATGACCAG
CTATCCGCTGTTGTGGCTCATGCAACCAATGCTGAAATTCTGGTTATTTTGAGCGATATTGACGGATATTTTGATAAAAA
TCCGCACGAATATCAAGATGCAAAAATCTTTAAAACCATGAGCAAGATACCTGAGCAAGCGCTACTTGATGAGTCGACTC
CTAATAACAGTTTTGCCACCGGAGGCATTGTTACAAAACTTAAGGCAGCTAACTTTCTTATAAGACATGGAAGAAAAATG
CTTCTGTGTAACGGTTTTGATCTAAGCGCCGCAGAGTCATTTTTGTTGGAAAATAAACACACTTTGGGTACTCTCTTTCT
GCCTGAAGAGGAGGCTAAAAAGTCGTCTGAAAGCGCGAGCAAAAAGTCAGCTGAAAACGCAATCAAAAAAGAGAGCTAA

Upstream 100 bases:

>100_bases
TAACCGATTAGAACGTAACCGATTAGCATATAACCGATTAGAACGTAACCGATTAGCACATAACCGATTAGCAAATAACT
TCAAAGGAAAACAGACGAAC

Downstream 100 bases:

>100_bases
TAGCTTTTGACTTAAGTCTGTTACGGAAAAATAACCACTGCTAACCGGTTTTTTTCGTAACCAACTTATAAATTGATCAG
ATCTTAATTTAAAGGTGCTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNTNTNTNTKPKTKNNTKRVVIKVGSAVLTDNNNIAKERMLNLVSMISGLRKTYDVILVTSGAVAAGYSTLKLDKSKQIG
KKALAAAGQPILMTAYKKKFDIYDIDTAQILLTEDDFDSRKRTKMFQEIIDAHLTHDILPIVNENDITSTPEQQFGDNDQ
LSAVVAHATNAEILVILSDIDGYFDKNPHEYQDAKIFKTMSKIPEQALLDESTPNNSFATGGIVTKLKAANFLIRHGRKM
LLCNGFDLSAAESFLLENKHTLGTLFLPEEEAKKSSESASKKSAENAIKKES

Sequences:

>Translated_292_residues
MNTNTNTNTKPKTKNNTKRVVIKVGSAVLTDNNNIAKERMLNLVSMISGLRKTYDVILVTSGAVAAGYSTLKLDKSKQIG
KKALAAAGQPILMTAYKKKFDIYDIDTAQILLTEDDFDSRKRTKMFQEIIDAHLTHDILPIVNENDITSTPEQQFGDNDQ
LSAVVAHATNAEILVILSDIDGYFDKNPHEYQDAKIFKTMSKIPEQALLDESTPNNSFATGGIVTKLKAANFLIRHGRKM
LLCNGFDLSAAESFLLENKHTLGTLFLPEEEAKKSSESASKKSAENAIKKES
>Mature_292_residues
MNTNTNTNTKPKTKNNTKRVVIKVGSAVLTDNNNIAKERMLNLVSMISGLRKTYDVILVTSGAVAAGYSTLKLDKSKQIG
KKALAAAGQPILMTAYKKKFDIYDIDTAQILLTEDDFDSRKRTKMFQEIIDAHLTHDILPIVNENDITSTPEQQFGDNDQ
LSAVVAHATNAEILVILSDIDGYFDKNPHEYQDAKIFKTMSKIPEQALLDESTPNNSFATGGIVTKLKAANFLIRHGRKM
LLCNGFDLSAAESFLLENKHTLGTLFLPEEEAKKSSESASKKSAENAIKKES

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=263, Percent_Identity=31.1787072243346, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI21361368, Length=265, Percent_Identity=30.9433962264151, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786437, Length=237, Percent_Identity=32.0675105485232, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=319, Percent_Identity=31.0344827586207, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=303, Percent_Identity=31.023102310231, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321822, Length=222, Percent_Identity=35.1351351351351, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=233, Percent_Identity=35.1931330472103, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357643, Length=308, Percent_Identity=31.4935064935065, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 32213; Mature: 32213

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNTNTNTKPKTKNNTKRVVIKVGSAVLTDNNNIAKERMLNLVSMISGLRKTYDVILVT
CCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
SGAVAAGYSTLKLDKSKQIGKKALAAAGQPILMTAYKKKFDIYDIDTAQILLTEDDFDSR
CCCHHHCCHHEEECCHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCCEEEEEECCCCCHH
KRTKMFQEIIDAHLTHDILPIVNENDITSTPEQQFGDNDQLSAVVAHATNAEILVILSDI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHEEEEEECCCCEEEEEEECC
DGYFDKNPHEYQDAKIFKTMSKIPEQALLDESTPNNSFATGGIVTKLKAANFLIRHGRKM
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCEE
LLCNGFDLSAAESFLLENKHTLGTLFLPEEEAKKSSESASKKSAENAIKKES
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTNTNTNTKPKTKNNTKRVVIKVGSAVLTDNNNIAKERMLNLVSMISGLRKTYDVILVT
CCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
SGAVAAGYSTLKLDKSKQIGKKALAAAGQPILMTAYKKKFDIYDIDTAQILLTEDDFDSR
CCCHHHCCHHEEECCHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCCEEEEEECCCCCHH
KRTKMFQEIIDAHLTHDILPIVNENDITSTPEQQFGDNDQLSAVVAHATNAEILVILSDI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHEEEEEECCCCEEEEEEECC
DGYFDKNPHEYQDAKIFKTMSKIPEQALLDESTPNNSFATGGIVTKLKAANFLIRHGRKM
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCEE
LLCNGFDLSAAESFLLENKHTLGTLFLPEEEAKKSSESASKKSAENAIKKES
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]