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The map label for this gene is aarA [H]

Identifier: 119946255

GI number: 119946255

Start: 3209375

End: 3210685

Strand: Reverse

Name: aarA [H]

Synonym: Ping_2617

Alternate gene names: 119946255

Gene position: 3210685-3209375 (Counterclockwise)

Preceding gene: 119946256

Following gene: 119946254

Centisome position: 70.42

GC content: 43.94

Gene sequence:

>1311_bases
ATGAAGGTACACAGTCTCACCATTACCGATAACCGTACCGGTAAAAGTTACGAAATTCCTATTCGATATGGCACCTACCC
TAACTATGGCGCATCCATTAATGCGCTTGAACTCCGCAACATAAAAGTATCTGAACAAGACTTTGGCCTGTTAACCTATG
ATCCTGGTTATACAAATACCGCTTCCTGTAAGAGCAGTGTGACATTTCTTGATGGCGAAAAGGGCATTCTTCGTTATCGG
GGTTACCCTATTGAGCAATTAGCAGAAAAAAGTACTTATTTAGAAACCGCCTATCTAATCTTAAACGGTGAGCTGCCTAA
CCAAACTCAGTTGAATGGGTGGCATAAAGATATTATGGCGCATACTTTTATACATGAAAACATCAAAAAATTGATAGACA
GTTTTCATTATGATGCACACCCTATGGGTACTTTAGTCGGTACTGTGGCGGCGTTATCTACGTTTTACCCGGATGCCAAA
AATATTCATGATCAAGCCTGTCGGGATAGGCAAATTTGGCGCTTAATTGCCAAGATGCCGACCCTTGCGGCCTTTTCTTA
TCGGCATAGTATTGGACTGCCTTATGTTTACCCGGATCAAAGTCTCTCTTACATTGGCAATTTCATGAGTATGTTATGGA
AAAAAAGTGAACTGCAATATCAGCCAAATCCGATTTTAGAAAAAGCCATCAATGTACTTTTTATTCTGCATGCGGATCAT
GAACAGAATTGTGGTACTAGCGTTATGCGCGCCGTGGCCAGCGCTTACAGCGATCCTTTCTCGGCGACCTCCTCGGCAAT
AGCTGCATTGTACGGGCCTTTGCATGGTGGTGCGAATGAGGCGGTATTGCATATGTTAGCGGAGATCGGTTCCGTCGATC
GTGTACCTGAGTTTATTAAGACTGTAAAATCCGGCGAAGTGAGTCGTTTGATGGGGTTTGGTCATCGTGTCTATAAAGAG
TATGATCCTCGCGCAAAAATTTGTAAGACATTAGCAAATGAAGTTTTTTCTGTAACGGGTGTCAATCCACTACTGGCTAT
TGCCCTGGAACTTGAGAGAGTGGCACTACAGGATGACTATTTTATCTCCCGTCATCTCTACCCTAACGTTGATTTTTATT
CCGGCTTGATTTATCAGGCAATGGGATTCCCCGTCGGAATGTTCCCCGTCCTGTTTGCTATTGCGCGTAATACGGGCTGG
CTGGCGCAATGGCAGGAATTTCTTGTCGATCCTGACCATAAAATAGCCCGCCCCAGACAAATCTATTGTGGCAGTGAAGT
GCGCGATTATATTGCAGTCGATAAGCGTTGA

Upstream 100 bases:

>100_bases
TTAGTTAGCTGCCGCTGGTTGAAAAGTAACCAGAAGAAGCTAAACTTTAATCTGTGTAGCAAATGGGTTCCTTAGTCATT
AAATTAGGGGGAGTGATATT

Downstream 100 bases:

>100_bases
AAGCAGATCAGGAAAGAGGATCTTCTGTGCAACTAGGGCTTGTTTAAATAAAAGTTAAATAAGCCCGCAGAGCATTTTAT
TTGCTAATACCTTATTATTT

Product: citrate synthase I

Products: NA

Alternate protein names: Acetic acid resistance protein [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKVHSLTITDNRTGKSYEIPIRYGTYPNYGASINALELRNIKVSEQDFGLLTYDPGYTNTASCKSSVTFLDGEKGILRYR
GYPIEQLAEKSTYLETAYLILNGELPNQTQLNGWHKDIMAHTFIHENIKKLIDSFHYDAHPMGTLVGTVAALSTFYPDAK
NIHDQACRDRQIWRLIAKMPTLAAFSYRHSIGLPYVYPDQSLSYIGNFMSMLWKKSELQYQPNPILEKAINVLFILHADH
EQNCGTSVMRAVASAYSDPFSATSSAIAALYGPLHGGANEAVLHMLAEIGSVDRVPEFIKTVKSGEVSRLMGFGHRVYKE
YDPRAKICKTLANEVFSVTGVNPLLAIALELERVALQDDYFISRHLYPNVDFYSGLIYQAMGFPVGMFPVLFAIARNTGW
LAQWQEFLVDPDHKIARPRQIYCGSEVRDYIAVDKR

Sequences:

>Translated_436_residues
MKVHSLTITDNRTGKSYEIPIRYGTYPNYGASINALELRNIKVSEQDFGLLTYDPGYTNTASCKSSVTFLDGEKGILRYR
GYPIEQLAEKSTYLETAYLILNGELPNQTQLNGWHKDIMAHTFIHENIKKLIDSFHYDAHPMGTLVGTVAALSTFYPDAK
NIHDQACRDRQIWRLIAKMPTLAAFSYRHSIGLPYVYPDQSLSYIGNFMSMLWKKSELQYQPNPILEKAINVLFILHADH
EQNCGTSVMRAVASAYSDPFSATSSAIAALYGPLHGGANEAVLHMLAEIGSVDRVPEFIKTVKSGEVSRLMGFGHRVYKE
YDPRAKICKTLANEVFSVTGVNPLLAIALELERVALQDDYFISRHLYPNVDFYSGLIYQAMGFPVGMFPVLFAIARNTGW
LAQWQEFLVDPDHKIARPRQIYCGSEVRDYIAVDKR
>Mature_436_residues
MKVHSLTITDNRTGKSYEIPIRYGTYPNYGASINALELRNIKVSEQDFGLLTYDPGYTNTASCKSSVTFLDGEKGILRYR
GYPIEQLAEKSTYLETAYLILNGELPNQTQLNGWHKDIMAHTFIHENIKKLIDSFHYDAHPMGTLVGTVAALSTFYPDAK
NIHDQACRDRQIWRLIAKMPTLAAFSYRHSIGLPYVYPDQSLSYIGNFMSMLWKKSELQYQPNPILEKAINVLFILHADH
EQNCGTSVMRAVASAYSDPFSATSSAIAALYGPLHGGANEAVLHMLAEIGSVDRVPEFIKTVKSGEVSRLMGFGHRVYKE
YDPRAKICKTLANEVFSVTGVNPLLAIALELERVALQDDYFISRHLYPNVDFYSGLIYQAMGFPVGMFPVLFAIARNTGW
LAQWQEFLVDPDHKIARPRQIYCGSEVRDYIAVDKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=411, Percent_Identity=27.4939172749392, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786939, Length=390, Percent_Identity=50.7692307692308, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=33.1578947368421, Blast_Score=178, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=446, Percent_Identity=26.0089686098655, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319850, Length=393, Percent_Identity=27.9898218829517, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324328, Length=363, Percent_Identity=28.3746556473829, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325257, Length=398, Percent_Identity=26.6331658291457, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640126, Length=419, Percent_Identity=28.1622911694511, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640124, Length=419, Percent_Identity=28.1622911694511, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356863, Length=397, Percent_Identity=28.463476070529, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49194; Mature: 49194

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVHSLTITDNRTGKSYEIPIRYGTYPNYGASINALELRNIKVSEQDFGLLTYDPGYTNT
CCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEEECEEECCCCCCEEEECCCCCCC
ASCKSSVTFLDGEKGILRYRGYPIEQLAEKSTYLETAYLILNGELPNQTQLNGWHKDIMA
CCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHH
HTFIHENIKKLIDSFHYDAHPMGTLVGTVAALSTFYPDAKNIHDQACRDRQIWRLIAKMP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
TLAAFSYRHSIGLPYVYPDQSLSYIGNFMSMLWKKSELQYQPNPILEKAINVLFILHADH
HHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEECCC
EQNCGTSVMRAVASAYSDPFSATSSAIAALYGPLHGGANEAVLHMLAEIGSVDRVPEFIK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
TVKSGEVSRLMGFGHRVYKEYDPRAKICKTLANEVFSVTGVNPLLAIALELERVALQDDY
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
FISRHLYPNVDFYSGLIYQAMGFPVGMFPVLFAIARNTGWLAQWQEFLVDPDHKIARPRQ
EEHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHCCCCE
IYCGSEVRDYIAVDKR
EECCHHHHHHHHCCCC
>Mature Secondary Structure
MKVHSLTITDNRTGKSYEIPIRYGTYPNYGASINALELRNIKVSEQDFGLLTYDPGYTNT
CCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEEECEEECCCCCCEEEECCCCCCC
ASCKSSVTFLDGEKGILRYRGYPIEQLAEKSTYLETAYLILNGELPNQTQLNGWHKDIMA
CCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHH
HTFIHENIKKLIDSFHYDAHPMGTLVGTVAALSTFYPDAKNIHDQACRDRQIWRLIAKMP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
TLAAFSYRHSIGLPYVYPDQSLSYIGNFMSMLWKKSELQYQPNPILEKAINVLFILHADH
HHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEEECCC
EQNCGTSVMRAVASAYSDPFSATSSAIAALYGPLHGGANEAVLHMLAEIGSVDRVPEFIK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
TVKSGEVSRLMGFGHRVYKEYDPRAKICKTLANEVFSVTGVNPLLAIALELERVALQDDY
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
FISRHLYPNVDFYSGLIYQAMGFPVGMFPVLFAIARNTGWLAQWQEFLVDPDHKIARPRQ
EEHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHCCCCE
IYCGSEVRDYIAVDKR
EECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156811 [H]