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The map label for this gene is setA [H]

Identifier: 119946187

GI number: 119946187

Start: 3115241

End: 3116449

Strand: Direct

Name: setA [H]

Synonym: Ping_2547

Alternate gene names: 119946187

Gene position: 3115241-3116449 (Clockwise)

Preceding gene: 119946185

Following gene: 119946189

Centisome position: 68.32

GC content: 40.53

Gene sequence:

>1209_bases
ATGTTCTTTAGTAAACAATCAATCACCTATTTATTGACCTGTTTTTTTATCGGGCTAAGTACCGCGTTTATTTATCCCCT
ATTAAGTCTTTATCTGATTGAAGAAGTTCATGCCACCCCGATCAGCATGGGTATTTTAATTACCTGCATGGTGTTAAGTG
GCGTATTGGTCTCGCAATATATAGCAAAAAAATCAGATCAGGGCTTAAGTCGCAAGTATATTATTTTAGCAGGACAAGTC
GGGTTTATTATCACTACGATTTTTTTGGCAATGAGTCAGCACTATTACACGCTATTATTTGCGGTTGTTTTTTTTATGCC
CTTCAGTGCGGCTTGTTTACCACAAATTTTCAGCATGGGACGTCATTTTGCAGATAAACAGCTTGCTGATAAAGCGACTG
TATTTATGACCATGATGCGTGCAACCATGTCGATGTCTTGGGTGATTGGGCCACCCGTAGCCTTTTTAATCAATGATGCT
TTCGGGTTTAAACAAACCTTTCTGCTGGCAGGATTTTTTGCTGTGGTCGTCTTAATTATTGTTTGTGTGGGGATATCTAA
TGTGCAGATTGAAAAGGTCAAGTCTGCTCCAAAAAATGTGAATTGGTCAAAGATATCTGGCGTTTTATTCTTTTTGATCT
GTATCTTTTTTATCTTTACTGCCAACAATATGTACATTACATCAATCAGCCTTTATATCACTCAAGCGTTAGATTTTGAT
TCTAAATGGGTGGGTTACCTGATGGGCACGGCGGCCTTTGTGGAAATTCCAATAATGTTAGGCGCAGGCTATCTTGCGCC
AAGGTTCGGTGCAACGCGACTGATCAATATTGCGATTATTAGCGGCCTGCTATTTTTTGCGGGTTTGCTGGTTGCAGAGC
AGCTCTGGCAGTTTATATTGCTGCAGCTGTTTAACGGTGTTTTTATTGGTATCAATGCCAGTTTAGGCATGCTGGTTGTA
CAGGATATGATGAAAAAGCAGATAGGGTTAGCCTCAACATTGTTTAATAACAGCCTCCTACTGAGCGTTTTATTAAGCAG
CCTTCTCGTGGGGGGAATCGCACAATATTTCAGTTATTATGCGGTATTTGTTGTCTCCGCCCTATTTTGTTTAATTGCCC
TGTTTTTTATGCGGTTAGCAGAAAGGCAATGCTTGTCTGTAAAGCCTGAAGGCTTAACGCCTGAAGGCCTTAAAGCCGGA
ACGATGTAA

Upstream 100 bases:

>100_bases
AAGGCGCACACTATGTTCTTTAGCAAAAAAATCAATTACTTATTGTATTTTAAATTATCAAATTGCGCTCGGAAGCCACT
CAATGACCAAGGTGCACACT

Downstream 100 bases:

>100_bases
AGCCGGAACGCTGGAACGCCTTATGGCAGCTGACAGCTGACAGCTAACGGCCCTATAACCTTCGCGCCTTCTAGCCTTAG
TACCTTCTTGCCTTAGGGCT

Product: major facilitator transporter

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MFFSKQSITYLLTCFFIGLSTAFIYPLLSLYLIEEVHATPISMGILITCMVLSGVLVSQYIAKKSDQGLSRKYIILAGQV
GFIITTIFLAMSQHYYTLLFAVVFFMPFSAACLPQIFSMGRHFADKQLADKATVFMTMMRATMSMSWVIGPPVAFLINDA
FGFKQTFLLAGFFAVVVLIIVCVGISNVQIEKVKSAPKNVNWSKISGVLFFLICIFFIFTANNMYITSISLYITQALDFD
SKWVGYLMGTAAFVEIPIMLGAGYLAPRFGATRLINIAIISGLLFFAGLLVAEQLWQFILLQLFNGVFIGINASLGMLVV
QDMMKKQIGLASTLFNNSLLLSVLLSSLLVGGIAQYFSYYAVFVVSALFCLIALFFMRLAERQCLSVKPEGLTPEGLKAG
TM

Sequences:

>Translated_402_residues
MFFSKQSITYLLTCFFIGLSTAFIYPLLSLYLIEEVHATPISMGILITCMVLSGVLVSQYIAKKSDQGLSRKYIILAGQV
GFIITTIFLAMSQHYYTLLFAVVFFMPFSAACLPQIFSMGRHFADKQLADKATVFMTMMRATMSMSWVIGPPVAFLINDA
FGFKQTFLLAGFFAVVVLIIVCVGISNVQIEKVKSAPKNVNWSKISGVLFFLICIFFIFTANNMYITSISLYITQALDFD
SKWVGYLMGTAAFVEIPIMLGAGYLAPRFGATRLINIAIISGLLFFAGLLVAEQLWQFILLQLFNGVFIGINASLGMLVV
QDMMKKQIGLASTLFNNSLLLSVLLSSLLVGGIAQYFSYYAVFVVSALFCLIALFFMRLAERQCLSVKPEGLTPEGLKAG
TM
>Mature_402_residues
MFFSKQSITYLLTCFFIGLSTAFIYPLLSLYLIEEVHATPISMGILITCMVLSGVLVSQYIAKKSDQGLSRKYIILAGQV
GFIITTIFLAMSQHYYTLLFAVVFFMPFSAACLPQIFSMGRHFADKQLADKATVFMTMMRATMSMSWVIGPPVAFLINDA
FGFKQTFLLAGFFAVVVLIIVCVGISNVQIEKVKSAPKNVNWSKISGVLFFLICIFFIFTANNMYITSISLYITQALDFD
SKWVGYLMGTAAFVEIPIMLGAGYLAPRFGATRLINIAIISGLLFFAGLLVAEQLWQFILLQLFNGVFIGINASLGMLVV
QDMMKKQIGLASTLFNNSLLLSVLLSSLLVGGIAQYFSYYAVFVVSALFCLIALFFMRLAERQCLSVKPEGLTPEGLKAG
TM

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=370, Percent_Identity=36.7567567567568, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788495, Length=382, Percent_Identity=34.8167539267016, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1790091, Length=373, Percent_Identity=33.5120643431635, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44415; Mature: 44415

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSKQSITYLLTCFFIGLSTAFIYPLLSLYLIEEVHATPISMGILITCMVLSGVLVSQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IAKKSDQGLSRKYIILAGQVGFIITTIFLAMSQHYYTLLFAVVFFMPFSAACLPQIFSMG
HHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RHFADKQLADKATVFMTMMRATMSMSWVIGPPVAFLINDAFGFKQTFLLAGFFAVVVLII
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VCVGISNVQIEKVKSAPKNVNWSKISGVLFFLICIFFIFTANNMYITSISLYITQALDFD
HHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCC
SKWVGYLMGTAAFVEIPIMLGAGYLAPRFGATRLINIAIISGLLFFAGLLVAEQLWQFIL
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLFNGVFIGINASLGMLVVQDMMKKQIGLASTLFNNSLLLSVLLSSLLVGGIAQYFSYY
HHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFVVSALFCLIALFFMRLAERQCLSVKPEGLTPEGLKAGTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFFSKQSITYLLTCFFIGLSTAFIYPLLSLYLIEEVHATPISMGILITCMVLSGVLVSQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IAKKSDQGLSRKYIILAGQVGFIITTIFLAMSQHYYTLLFAVVFFMPFSAACLPQIFSMG
HHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RHFADKQLADKATVFMTMMRATMSMSWVIGPPVAFLINDAFGFKQTFLLAGFFAVVVLII
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VCVGISNVQIEKVKSAPKNVNWSKISGVLFFLICIFFIFTANNMYITSISLYITQALDFD
HHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCC
SKWVGYLMGTAAFVEIPIMLGAGYLAPRFGATRLINIAIISGLLFFAGLLVAEQLWQFIL
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLFNGVFIGINASLGMLVVQDMMKKQIGLASTLFNNSLLLSVLLSSLLVGGIAQYFSYY
HHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFVVSALFCLIALFFMRLAERQCLSVKPEGLTPEGLKAGTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]