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The map label for this gene is ptsI [H]

Identifier: 119946090

GI number: 119946090

Start: 2988355

End: 2990076

Strand: Direct

Name: ptsI [H]

Synonym: Ping_2440

Alternate gene names: 119946090

Gene position: 2988355-2990076 (Clockwise)

Preceding gene: 119946089

Following gene: 119946091

Centisome position: 65.54

GC content: 38.33

Gene sequence:

>1722_bases
ATGATATCTGGCATTCTAGCATCTCCTGGTATAACAATAGCGAAGGCATTATTATTACAGGAAGATGAAATTACCCTTAA
TAAGGCAAAAGTGAGTGATTCTGATAAAGAAATCGCACGTTTTAAAGACGGGCGGGCAAAAACATCAGCTCAACTTGAAA
TTATCAAACAACAAGCACTTGAAAAATTTGGCGAAGAAAAAGAGGCGATCTTTGAAGGCCATCTGATGCTACTTGATGAT
GAAGAGCTAGAAGAAGATATCGTTTCCCACATTCGTGAAAATAAATGCAGTGCTGATGTTGCGATTAATTCTATTTTTGA
AGAAAATGCCAACATGCTTCAACAGCTAGAAGATCCTTACTTACGTGAACGTGCTTCTGATTTTCGTGATATCGGTAACC
GTTTACTGAAAAATGTACTGGGTATCAAAATTGTTGATTTAAGTGATATTACTGAAGAGGTTGTGTTAATTGCTAATGAT
TTAACACCATCAGAAACAGCACAAATCAACCTTGATAAAGTGCTGGGTTTTATCACTGATATTGGCGGCAGAACATCACA
TACTTCAATCATGGCACGTTCCCTCGAAATTCCTGCGATCGTTGGTACAAAAAATATTACTAAGCGTGTAAAAAATGGTG
ATTTAATCATTTTAGATGCACTTAATAATAAAATTACTATTAATCCGACGAATGCGGAAATTGAAGAAGCGAAACGCATC
AAAGCAGGCTTCTTGGCTGATAAAGCTGAACTTGCCAAATTGAAAGACTTGCCTGCAGTAACACTTGATGATCATCATGT
GGAAGTTTGTGCCAATATCGGCAGTGTTAAAGATACTGAAGGTGCAAAACGTAATGGTGCTGAGGGTGTTGGTCTGTACC
GCACTGAATTCTTATTTATGGATCGTGACCAGTTACCCACTGAACAAGAACAGTTTTTAGCTTATAAAGACGTTGTTGAA
GCAATGGATGGTCAAGCTTGTATTATACGTACAATGGATATTGGTGGCGATAAAGATTTACCCTACCTTAATTTGCCAAC
AGAAATGAATCCTTTCTTAGGATGGCGTGCAATCCGCATCTGTTTCGATCGACCAGAAATAATGAATCCTCAATTACGTG
CTTTATTGCGTGCATCTTCATTTGGTAAAATTCGTATTATGTTCCCGATGATAATCTCAGTTGAAGAAATTCGTCGACTA
AAAGAGATTTTGGATGAACTGAAAGAGGAACTTACTGCAGAAGGTGTCGATTTTGATAAAGAAATCGAAATCGGTGTTAT
GATCGAAACACCGGCAGCGGCAGCTATTGCGCATCACTTGATTAAAGAAGTTGATTTTTTCAGTATTGGCACAAATGATT
TAACCCAATACACCCTTGCTGTAGACCGCGGTAATGAACTTATCTCTGCTCTCTACAATCCAATGACACCTTCTGTATTA
AGCATTATCAAAACAGTGATTGATGCTTCTCATGCGGCAGGTAAATGGACGGGTATGTGTGGCGAATTAGCGGGTGATGA
AAAAGCGACATTATTGTTAGTCGGCATGGGCCTTGATGAGTTCTCCATGAGTGCTATGTCTATTCCAACGGTTAAGAAAA
TTATCCGTAACACTAATTATGCAGACGTAAAAGCACTTGCTGATCAAGCATTATCAGTCGCCACAGCAGCTGAAATTGAA
GCCTTAGTTAAAGATTTCAACAAAACTAAAGGTATTAACTAA

Upstream 100 bases:

>100_bases
TTTTAGGCTCTTTCTTATTGGATTACTGAAAAATAATCTTTTAATTACAATTACTTATAGAAAGAGTCCAAATTTTAATT
AGAACTTTAAAGGATGGATT

Downstream 100 bases:

>100_bases
ATATTTAAACGAACTACAATGTATATAAAAACAAAAATAGTTCATTTAAAAAATATAACTATTAACAATAATAGGGAGTT
CGACTATGGGTTTTTTTGAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MISGILASPGITIAKALLLQEDEITLNKAKVSDSDKEIARFKDGRAKTSAQLEIIKQQALEKFGEEKEAIFEGHLMLLDD
EELEEDIVSHIRENKCSADVAINSIFEENANMLQQLEDPYLRERASDFRDIGNRLLKNVLGIKIVDLSDITEEVVLIAND
LTPSETAQINLDKVLGFITDIGGRTSHTSIMARSLEIPAIVGTKNITKRVKNGDLIILDALNNKITINPTNAEIEEAKRI
KAGFLADKAELAKLKDLPAVTLDDHHVEVCANIGSVKDTEGAKRNGAEGVGLYRTEFLFMDRDQLPTEQEQFLAYKDVVE
AMDGQACIIRTMDIGGDKDLPYLNLPTEMNPFLGWRAIRICFDRPEIMNPQLRALLRASSFGKIRIMFPMIISVEEIRRL
KEILDELKEELTAEGVDFDKEIEIGVMIETPAAAAIAHHLIKEVDFFSIGTNDLTQYTLAVDRGNELISALYNPMTPSVL
SIIKTVIDASHAAGKWTGMCGELAGDEKATLLLVGMGLDEFSMSAMSIPTVKKIIRNTNYADVKALADQALSVATAAEIE
ALVKDFNKTKGIN

Sequences:

>Translated_573_residues
MISGILASPGITIAKALLLQEDEITLNKAKVSDSDKEIARFKDGRAKTSAQLEIIKQQALEKFGEEKEAIFEGHLMLLDD
EELEEDIVSHIRENKCSADVAINSIFEENANMLQQLEDPYLRERASDFRDIGNRLLKNVLGIKIVDLSDITEEVVLIAND
LTPSETAQINLDKVLGFITDIGGRTSHTSIMARSLEIPAIVGTKNITKRVKNGDLIILDALNNKITINPTNAEIEEAKRI
KAGFLADKAELAKLKDLPAVTLDDHHVEVCANIGSVKDTEGAKRNGAEGVGLYRTEFLFMDRDQLPTEQEQFLAYKDVVE
AMDGQACIIRTMDIGGDKDLPYLNLPTEMNPFLGWRAIRICFDRPEIMNPQLRALLRASSFGKIRIMFPMIISVEEIRRL
KEILDELKEELTAEGVDFDKEIEIGVMIETPAAAAIAHHLIKEVDFFSIGTNDLTQYTLAVDRGNELISALYNPMTPSVL
SIIKTVIDASHAAGKWTGMCGELAGDEKATLLLVGMGLDEFSMSAMSIPTVKKIIRNTNYADVKALADQALSVATAAEIE
ALVKDFNKTKGIN
>Mature_573_residues
MISGILASPGITIAKALLLQEDEITLNKAKVSDSDKEIARFKDGRAKTSAQLEIIKQQALEKFGEEKEAIFEGHLMLLDD
EELEEDIVSHIRENKCSADVAINSIFEENANMLQQLEDPYLRERASDFRDIGNRLLKNVLGIKIVDLSDITEEVVLIAND
LTPSETAQINLDKVLGFITDIGGRTSHTSIMARSLEIPAIVGTKNITKRVKNGDLIILDALNNKITINPTNAEIEEAKRI
KAGFLADKAELAKLKDLPAVTLDDHHVEVCANIGSVKDTEGAKRNGAEGVGLYRTEFLFMDRDQLPTEQEQFLAYKDVVE
AMDGQACIIRTMDIGGDKDLPYLNLPTEMNPFLGWRAIRICFDRPEIMNPQLRALLRASSFGKIRIMFPMIISVEEIRRL
KEILDELKEELTAEGVDFDKEIEIGVMIETPAAAAIAHHLIKEVDFFSIGTNDLTQYTLAVDRGNELISALYNPMTPSVL
SIIKTVIDASHAAGKWTGMCGELAGDEKATLLLVGMGLDEFSMSAMSIPTVKKIIRNTNYADVKALADQALSVATAAEIE
ALVKDFNKTKGIN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=68.2926829268293, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=40.4217926186292, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI48994992, Length=509, Percent_Identity=37.721021611002, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI1789193, Length=584, Percent_Identity=32.0205479452055, Blast_Score=295, Evalue=7e-81,
Organism=Escherichia coli, GI1787994, Length=445, Percent_Identity=25.3932584269663, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI226510935, Length=129, Percent_Identity=32.5581395348837, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63240; Mature: 63240

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGITIAKALLLQEDEITLNKAKVSDSDKEIARFKDGRAKTSAQLEIIKQQAL
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
EKFGEEKEAIFEGHLMLLDDEELEEDIVSHIRENKCSADVAINSIFEENANMLQQLEDPY
HHHCCHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHH
LRERASDFRDIGNRLLKNVLGIKIVDLSDITEEVVLIANDLTPSETAQINLDKVLGFITD
HHHHHHHHHHHHHHHHHHHHCEEEEEHHHHCCEEEEEECCCCCCCCCEEEHHHHHHHHHH
IGGRTSHTSIMARSLEIPAIVGTKNITKRVKNGDLIILDALNNKITINPTNAEIEEAKRI
CCCCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KAGFLADKAELAKLKDLPAVTLDDHHVEVCANIGSVKDTEGAKRNGAEGVGLYRTEFLFM
HHHHHCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
DRDQLPTEQEQFLAYKDVVEAMDGQACIIRTMDIGGDKDLPYLNLPTEMNPFLGWRAIRI
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCCCCCHHCCEEEEE
CFDRPEIMNPQLRALLRASSFGKIRIMFPMIISVEEIRRLKEILDELKEELTAEGVDFDK
EECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EIEIGVMIETPAAAAIAHHLIKEVDFFSIGTNDLTQYTLAVDRGNELISALYNPMTPSVL
CEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCHHHHHHHHCCCCHHHH
SIIKTVIDASHAAGKWTGMCGELAGDEKATLLLVGMGLDEFSMSAMSIPTVKKIIRNTNY
HHHHHHHHCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHCCCH
ADVKALADQALSVATAAEIEALVKDFNKTKGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGITIAKALLLQEDEITLNKAKVSDSDKEIARFKDGRAKTSAQLEIIKQQAL
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
EKFGEEKEAIFEGHLMLLDDEELEEDIVSHIRENKCSADVAINSIFEENANMLQQLEDPY
HHHCCHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHH
LRERASDFRDIGNRLLKNVLGIKIVDLSDITEEVVLIANDLTPSETAQINLDKVLGFITD
HHHHHHHHHHHHHHHHHHHHCEEEEEHHHHCCEEEEEECCCCCCCCCEEEHHHHHHHHHH
IGGRTSHTSIMARSLEIPAIVGTKNITKRVKNGDLIILDALNNKITINPTNAEIEEAKRI
CCCCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KAGFLADKAELAKLKDLPAVTLDDHHVEVCANIGSVKDTEGAKRNGAEGVGLYRTEFLFM
HHHHHCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
DRDQLPTEQEQFLAYKDVVEAMDGQACIIRTMDIGGDKDLPYLNLPTEMNPFLGWRAIRI
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCCCCCHHCCEEEEE
CFDRPEIMNPQLRALLRASSFGKIRIMFPMIISVEEIRRLKEILDELKEELTAEGVDFDK
EECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EIEIGVMIETPAAAAIAHHLIKEVDFFSIGTNDLTQYTLAVDRGNELISALYNPMTPSVL
CEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCHHHHHHHHCCCCHHHH
SIIKTVIDASHAAGKWTGMCGELAGDEKATLLLVGMGLDEFSMSAMSIPTVKKIIRNTNY
HHHHHHHHCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHCCCH
ADVKALADQALSVATAAEIEALVKDFNKTKGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]