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The map label for this gene is gatA [H]

Identifier: 119946077

GI number: 119946077

Start: 2974600

End: 2975796

Strand: Reverse

Name: gatA [H]

Synonym: Ping_2426

Alternate gene names: 119946077

Gene position: 2975796-2974600 (Counterclockwise)

Preceding gene: 119946078

Following gene: 119946076

Centisome position: 65.26

GC content: 47.37

Gene sequence:

>1197_bases
ATGAATGGATTGATAGATAAGGACAGCCGTGTTTATTGCCGGCAGGGGCCGCAAAGGCTATTGCCCATTAGCGAGGGTTT
ACTGTCGGGTTATACTTTTGTTTTTAAAGATTTGTTTGATGTCAAAGGTTATGTTACCGGCGCGGGTAATCCGACCTGGC
TTGAGACTCACAATATGGCAGAGCAAACATCGCCACTGATTTTAGCGCTGTTAAGTCAAGGAGCAAATTGCGTTGGGCGA
GTACAAAGTGATGAGCTTGCTTATAGTTTAAATGGTCAAAATATTCATTACGGTACTCCTGTTAATCCTATCGCGCCGAA
TTGTCTTCCGGGTGGATCATCCAGTGGCAGTGCCGTAGCTGTTGCCAGTAAGGATGCTGATTTTTCTATTGGCACCGATA
CCGGTGGTTCGGTTCGTGTACCCGCCAGTTACTGCGGGTTATATGGTTTAAGGCCGACCTTGGCAAAGTTTAATCTTGAG
CACTGTTTTGAGCTGGCAAAGAGCTTTGATACTGCGGGTATCTTTACGCGAGATCTGGATTTAATGCATAAAGTATTCGA
GGTGCTTAATGGCGCACCGGTTAAGGGGGAATCTGCCCAAACTCTTTATCTGGATAAGACTCTGGCAGCGCAGCTTAGTG
ATCAGCGTTTAAAGCGTTTACAAGTTTGCTGCATTGAGGCTGGGATCACCCTGCTTAATGGTGATTTTCTGGCTAAGTAT
CATCTTAGCCTTGAAGCCATCAGTTTGTTATTTCGCACTGTTCAGGGTTATGAAATCATTGCAAAGCATCGCGAGTGGTT
AGCTGAGTTCGGACACAGTTTAGAGCCTTCTATTTACCAACGGGTGGAATGGTCACGCACGATCAGTGCAGAGCAATATA
CCCAGGCTAAAAAACGTCAAACAGATTTCAGGCTGCAACTGACCGTTCTGCTTCAGCAGCAAGGGGGGTTGTGGTTAGTG
CCAACAACACCTTCAGGGCCACCAAGCCTCTCTCTTGCAGGCGATCAGCTGGCCAAGTATCGATCGAACTTAATGGGACT
CACCAGTATTGCAGGGCTGAGCGGTTTCCCGCAACTGCATCTGCCTATCAAGGGCCTAGAAGAAGGGCCCTGCGGAATAT
CACTGTTAGGTCTGCCGGACAGTGAAGGAGATCTGTTCGCTACCGCGCAACAGTTAATTCAAGGAAAACAATTATGA

Upstream 100 bases:

>100_bases
TATACAAATTTTCTACTGCGCAATGTTGGCTAACCGTTATGTAAAACAAAGTGAAAAGAACCAACAGCTAGCGGCTAAGC
AGCCATAAGGAAGGTTAATA

Downstream 100 bases:

>100_bases
ACTATCAATCAGCATTTAGCGCATCGCATTATCAAGCCACTCAAACAGGTCAAGCTATATTAGATGCAGGAGGCACTGCA
AGCGAAGCGATGGTTGCAGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNGLIDKDSRVYCRQGPQRLLPISEGLLSGYTFVFKDLFDVKGYVTGAGNPTWLETHNMAEQTSPLILALLSQGANCVGR
VQSDELAYSLNGQNIHYGTPVNPIAPNCLPGGSSSGSAVAVASKDADFSIGTDTGGSVRVPASYCGLYGLRPTLAKFNLE
HCFELAKSFDTAGIFTRDLDLMHKVFEVLNGAPVKGESAQTLYLDKTLAAQLSDQRLKRLQVCCIEAGITLLNGDFLAKY
HLSLEAISLLFRTVQGYEIIAKHREWLAEFGHSLEPSIYQRVEWSRTISAEQYTQAKKRQTDFRLQLTVLLQQQGGLWLV
PTTPSGPPSLSLAGDQLAKYRSNLMGLTSIAGLSGFPQLHLPIKGLEEGPCGISLLGLPDSEGDLFATAQQLIQGKQL

Sequences:

>Translated_398_residues
MNGLIDKDSRVYCRQGPQRLLPISEGLLSGYTFVFKDLFDVKGYVTGAGNPTWLETHNMAEQTSPLILALLSQGANCVGR
VQSDELAYSLNGQNIHYGTPVNPIAPNCLPGGSSSGSAVAVASKDADFSIGTDTGGSVRVPASYCGLYGLRPTLAKFNLE
HCFELAKSFDTAGIFTRDLDLMHKVFEVLNGAPVKGESAQTLYLDKTLAAQLSDQRLKRLQVCCIEAGITLLNGDFLAKY
HLSLEAISLLFRTVQGYEIIAKHREWLAEFGHSLEPSIYQRVEWSRTISAEQYTQAKKRQTDFRLQLTVLLQQQGGLWLV
PTTPSGPPSLSLAGDQLAKYRSNLMGLTSIAGLSGFPQLHLPIKGLEEGPCGISLLGLPDSEGDLFATAQQLIQGKQL
>Mature_398_residues
MNGLIDKDSRVYCRQGPQRLLPISEGLLSGYTFVFKDLFDVKGYVTGAGNPTWLETHNMAEQTSPLILALLSQGANCVGR
VQSDELAYSLNGQNIHYGTPVNPIAPNCLPGGSSSGSAVAVASKDADFSIGTDTGGSVRVPASYCGLYGLRPTLAKFNLE
HCFELAKSFDTAGIFTRDLDLMHKVFEVLNGAPVKGESAQTLYLDKTLAAQLSDQRLKRLQVCCIEAGITLLNGDFLAKY
HLSLEAISLLFRTVQGYEIIAKHREWLAEFGHSLEPSIYQRVEWSRTISAEQYTQAKKRQTDFRLQLTVLLQQQGGLWLV
PTTPSGPPSLSLAGDQLAKYRSNLMGLTSIAGLSGFPQLHLPIKGLEEGPCGISLLGLPDSEGDLFATAQQLIQGKQL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17543272, Length=401, Percent_Identity=26.1845386533666, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=198, Percent_Identity=30.8080808080808, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=183, Percent_Identity=30.0546448087432, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=24.6753246753247, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43210; Mature: 43210

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLIDKDSRVYCRQGPQRLLPISEGLLSGYTFVFKDLFDVKGYVTGAGNPTWLETHNMA
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCCCH
EQTSPLILALLSQGANCVGRVQSDELAYSLNGQNIHYGTPVNPIAPNCLPGGSSSGSAVA
HHCCHHHHHHHHCCCCEEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCEEE
VASKDADFSIGTDTGGSVRVPASYCGLYGLRPTLAKFNLEHCFELAKSFDTAGIFTRDLD
EEECCCCEEECCCCCCCEECCHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHH
LMHKVFEVLNGAPVKGESAQTLYLDKTLAAQLSDQRLKRLQVCCIEAGITLLNGDFLAKY
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
HLSLEAISLLFRTVQGYEIIAKHREWLAEFGHSLEPSIYQRVEWSRTISAEQYTQAKKRQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
TDFRLQLTVLLQQQGGLWLVPTTPSGPPSLSLAGDQLAKYRSNLMGLTSIAGLSGFPQLH
CCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LPIKGLEEGPCGISLLGLPDSEGDLFATAQQLIQGKQL
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNGLIDKDSRVYCRQGPQRLLPISEGLLSGYTFVFKDLFDVKGYVTGAGNPTWLETHNMA
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCCCH
EQTSPLILALLSQGANCVGRVQSDELAYSLNGQNIHYGTPVNPIAPNCLPGGSSSGSAVA
HHCCHHHHHHHHCCCCEEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCEEE
VASKDADFSIGTDTGGSVRVPASYCGLYGLRPTLAKFNLEHCFELAKSFDTAGIFTRDLD
EEECCCCEEECCCCCCCEECCHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHH
LMHKVFEVLNGAPVKGESAQTLYLDKTLAAQLSDQRLKRLQVCCIEAGITLLNGDFLAKY
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
HLSLEAISLLFRTVQGYEIIAKHREWLAEFGHSLEPSIYQRVEWSRTISAEQYTQAKKRQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
TDFRLQLTVLLQQQGGLWLVPTTPSGPPSLSLAGDQLAKYRSNLMGLTSIAGLSGFPQLH
CCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LPIKGLEEGPCGISLLGLPDSEGDLFATAQQLIQGKQL
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA