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The map label for this gene is cysL [H]

Identifier: 119946035

GI number: 119946035

Start: 2895981

End: 2896376

Strand: Direct

Name: cysL [H]

Synonym: Ping_2379

Alternate gene names: 119946035

Gene position: 2895981-2896376 (Clockwise)

Preceding gene: 119946023

Following gene: 119946036

Centisome position: 63.51

GC content: 41.67

Gene sequence:

>396_bases
ATGTTCAAGTTAATGCTTCAATTCATTCAGGTCGCAAAAGCACAAAATGTGACACAAGCTGCCAACAAACTCTGTTTAAG
CCAACCTACCTTAAGTCATAACATGAATAAATTAGAGGAAAAGCTTGAAACCAAGTTATTTAATCGCAACTCTAAAGGGA
TCACGCTCACGTCAAGTGGGAAACTGCTCTATGAGCAAGCGAAAATGATGCAGCACCTCTATCAAAATACCCTCAATAAG
CTCGATAAAAATAAGCTCAGGCATCAATACGAACTTAAAATAGGCTGCGGTGACGCTTGGTGGCACCTCTTTATTCGCGA
CTGTGTGCAGAACTTCCAAAATGCTTACCCGCACAGCAATATCACCATCGACGTAGGTGATCATCTGCAGCAATGA

Upstream 100 bases:

>100_bases
TGAAAAATTTCTGACACAGTGAGCGATGCCAACATAGCCATACAAAAACACTATTATAATGGCCAGATCATAGAATTTAT
CTATTACGACAAGGGCAGCA

Downstream 100 bases:

>100_bases
GGATATGGCGCACTTTCCCTCGGTTCATCTAGGGACGTCGAAAAAACGCTTTGCCCATTTGCAGCAGCAAAGCCCTGAAA
TGAGATCTTTTCTCAAACGC

Product: transcriptional regulator of LysR family protein

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MFKLMLQFIQVAKAQNVTQAANKLCLSQPTLSHNMNKLEEKLETKLFNRNSKGITLTSSGKLLYEQAKMMQHLYQNTLNK
LDKNKLRHQYELKIGCGDAWWHLFIRDCVQNFQNAYPHSNITIDVGDHLQQ

Sequences:

>Translated_131_residues
MFKLMLQFIQVAKAQNVTQAANKLCLSQPTLSHNMNKLEEKLETKLFNRNSKGITLTSSGKLLYEQAKMMQHLYQNTLNK
LDKNKLRHQYELKIGCGDAWWHLFIRDCVQNFQNAYPHSNITIDVGDHLQQ
>Mature_131_residues
MFKLMLQFIQVAKAQNVTQAANKLCLSQPTLSHNMNKLEEKLETKLFNRNSKGITLTSSGKLLYEQAKMMQHLYQNTLNK
LDKNKLRHQYELKIGCGDAWWHLFIRDCVQNFQNAYPHSNITIDVGDHLQQ

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=122, Percent_Identity=28.6885245901639, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=119, Percent_Identity=27.7310924369748, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=119, Percent_Identity=26.890756302521, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 15278; Mature: 15278

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLMLQFIQVAKAQNVTQAANKLCLSQPTLSHNMNKLEEKLETKLFNRNSKGITLTSSG
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
KLLYEQAKMMQHLYQNTLNKLDKNKLRHQYELKIGCGDAWWHLFIRDCVQNFQNAYPHSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCC
ITIDVGDHLQQ
EEEECCCCCCC
>Mature Secondary Structure
MFKLMLQFIQVAKAQNVTQAANKLCLSQPTLSHNMNKLEEKLETKLFNRNSKGITLTSSG
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
KLLYEQAKMMQHLYQNTLNKLDKNKLRHQYELKIGCGDAWWHLFIRDCVQNFQNAYPHSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCC
ITIDVGDHLQQ
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]