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The map label for this gene is rluF [H]

Identifier: 119945902

GI number: 119945902

Start: 2725933

End: 2726889

Strand: Reverse

Name: rluF [H]

Synonym: Ping_2242

Alternate gene names: 119945902

Gene position: 2726889-2725933 (Counterclockwise)

Preceding gene: 119945906

Following gene: 119945901

Centisome position: 59.81

GC content: 38.24

Gene sequence:

>957_bases
GTGCAAGGACATTTTTTGGGTTTTAAACGATTAATACAGGATAATATTGTGGATAATAATAGTGAAATACGGCTGAATAA
GTTTATCAGTGATTCAGGCTTTTGTTCACGCCGGGAAGCGGATAAGTTGATTGAGCAAAATCGTGTAAAAATAAATGGTG
ATATACCTGAATTAGGTACAAAAGTGCAACTTGGTGATCTCGTATTGGTCGACGGCAAGCGAATTAACGCCAGTGCTCAG
GATAAATTGGATCGTATCTATATTGTTTATAATAAACCGATCGGCATCACCTGCACGACAGAGCGTCATATCCATGGCAA
TATTATTAATGCTATTGGTCATAAAAAACGCATTTTTCCAATAGGACGACTAGATAAACCTTCTGAAGGTTTAATCTTTT
TAACCAGTGATGGTGATATAGTCAATAAAATATTACGTGCTGAAAATGCGCACGATAAAGAATATTTGGTCACCGTTGAT
AAACCGTTAAGTGAACGCTTTGTTGAGCGTATGCAGCGTGGTGTCCCTATCCTTGATACAATTACCAAGCCTTGTAAAGT
AACAGTAGACAGTCGCTTTATCTTTCGCATTGTCCTGACTCAGGGATTAAATCGCCAGATCAGGCGTATGTGTGAACACT
TAGGTTATGAAGTGCTGAAATTAAAACGAACACGCATTATGAAAGTAGGATTAGGCAATTTAAAACCAGGCCAATGGCGT
AATTTAACCAATATAGAACTGGCAGAAATAAACCAAGCGGTTTCTTCTTCGTCAAAGACAGCGACTGATCCTGCAAAACC
CGTTAGCAAAGCCCCACAATCGGAGGCCATAAAAAAATCAACGGGAGCCAATAATAGTCATCAGCCGGCAAAAAAAATAT
TTATCAATAAAAACAGCAAAAACACAACAACACCAACTGCCAATGTAAAAGCAACCTTGAAGTTAAAACGTAAATGA

Upstream 100 bases:

>100_bases
AGCTGTTTCATTTGCTCACGGTTTAATGTTTAATGTTCATAGCAAGTCATCGATACCCATTTTATTTATTACCCAAACGG
GCTGCGCCTTTTTAATCGCT

Downstream 100 bases:

>100_bases
ATCTCAGTGAATTTGATTTGATAGACAGCTATTTTAAACGCAGCACAACAGTTCAAAATAGTGGTGTGGCGTTAGGGATC
GGTGATGATTGTGCTCTTTT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQGHFLGFKRLIQDNIVDNNSEIRLNKFISDSGFCSRREADKLIEQNRVKINGDIPELGTKVQLGDLVLVDGKRINASAQ
DKLDRIYIVYNKPIGITCTTERHIHGNIINAIGHKKRIFPIGRLDKPSEGLIFLTSDGDIVNKILRAENAHDKEYLVTVD
KPLSERFVERMQRGVPILDTITKPCKVTVDSRFIFRIVLTQGLNRQIRRMCEHLGYEVLKLKRTRIMKVGLGNLKPGQWR
NLTNIELAEINQAVSSSSKTATDPAKPVSKAPQSEAIKKSTGANNSHQPAKKIFINKNSKNTTTPTANVKATLKLKRK

Sequences:

>Translated_318_residues
MQGHFLGFKRLIQDNIVDNNSEIRLNKFISDSGFCSRREADKLIEQNRVKINGDIPELGTKVQLGDLVLVDGKRINASAQ
DKLDRIYIVYNKPIGITCTTERHIHGNIINAIGHKKRIFPIGRLDKPSEGLIFLTSDGDIVNKILRAENAHDKEYLVTVD
KPLSERFVERMQRGVPILDTITKPCKVTVDSRFIFRIVLTQGLNRQIRRMCEHLGYEVLKLKRTRIMKVGLGNLKPGQWR
NLTNIELAEINQAVSSSSKTATDPAKPVSKAPQSEAIKKSTGANNSHQPAKKIFINKNSKNTTTPTANVKATLKLKRK
>Mature_318_residues
MQGHFLGFKRLIQDNIVDNNSEIRLNKFISDSGFCSRREADKLIEQNRVKINGDIPELGTKVQLGDLVLVDGKRINASAQ
DKLDRIYIVYNKPIGITCTTERHIHGNIINAIGHKKRIFPIGRLDKPSEGLIFLTSDGDIVNKILRAENAHDKEYLVTVD
KPLSERFVERMQRGVPILDTITKPCKVTVDSRFIFRIVLTQGLNRQIRRMCEHLGYEVLKLKRTRIMKVGLGNLKPGQWR
NLTNIELAEINQAVSSSSKTATDPAKPVSKAPQSEAIKKSTGANNSHQPAKKIFINKNSKNTTTPTANVKATLKLKRK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=56.4853556485356, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=31.1965811965812, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787524, Length=277, Percent_Identity=28.158844765343, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=26.1363636363636, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35794; Mature: 35794

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGHFLGFKRLIQDNIVDNNSEIRLNKFISDSGFCSRREADKLIEQNRVKINGDIPELGT
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCC
KVQLGDLVLVDGKRINASAQDKLDRIYIVYNKPIGITCTTERHIHGNIINAIGHKKRIFP
EEEECCEEEECCCEECCCHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHCCCCCEEEE
IGRLDKPSEGLIFLTSDGDIVNKILRAENAHDKEYLVTVDKPLSERFVERMQRGVPILDT
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHH
ITKPCKVTVDSRFIFRIVLTQGLNRQIRRMCEHLGYEVLKLKRTRIMKVGLGNLKPGQWR
CCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCC
NLTNIELAEINQAVSSSSKTATDPAKPVSKAPQSEAIKKSTGANNSHQPAKKIFINKNSK
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCC
NTTTPTANVKATLKLKRK
CCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MQGHFLGFKRLIQDNIVDNNSEIRLNKFISDSGFCSRREADKLIEQNRVKINGDIPELGT
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCC
KVQLGDLVLVDGKRINASAQDKLDRIYIVYNKPIGITCTTERHIHGNIINAIGHKKRIFP
EEEECCEEEECCCEECCCHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHCCCCCEEEE
IGRLDKPSEGLIFLTSDGDIVNKILRAENAHDKEYLVTVDKPLSERFVERMQRGVPILDT
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHH
ITKPCKVTVDSRFIFRIVLTQGLNRQIRRMCEHLGYEVLKLKRTRIMKVGLGNLKPGQWR
CCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCC
NLTNIELAEINQAVSSSSKTATDPAKPVSKAPQSEAIKKSTGANNSHQPAKKIFINKNSK
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCC
NTTTPTANVKATLKLKRK
CCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]