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The map label for this gene is purT

Identifier: 119945894

GI number: 119945894

Start: 2717843

End: 2719033

Strand: Direct

Name: purT

Synonym: Ping_2234

Alternate gene names: 119945894

Gene position: 2717843-2719033 (Clockwise)

Preceding gene: 119945893

Following gene: 119945895

Centisome position: 59.61

GC content: 42.99

Gene sequence:

>1191_bases
ATGAGTGTTTCAATTTTTGGCAGTGCAAAGGCTAATAGTGCGACAAAAGCCCTTCTTTTAGGATCTGGAGAACTGGGTAA
AGAAGTGGCAATTGAACTGCAACGCTTTGGTATTGAAGTGATTGCGGCAGATAGTTATGAAAATGCGCCTGCAATGCAAG
TCGCGCATTCATCCCATGTTGTTAGTATGCTGGATGGCGAAAAATTAGCGACAATTATTCGAGCCGAAAAGCCTGATTTT
ATTATCCCTGAAGTAGAAGCCATCGCCACGGATACATTACTTGCCTTAGAAGCAGAAGGTTTTAATGTAGTACCCACAGC
CCGTGCCGCAAAATTGACTATGGACAGAGAAGGTATTCGCCGCCTCGCTGCTGAAACCTTAGGAATAAAAACATCACCCT
ATATATTTGCAGACAGCAAAGAAGAATATTTATCTGCGATCGAGAAAATAGGCAAACCTTGTGTGATTAAACCCGTTATG
AGCAGTTCAGGTAAGGGCCAAAGTATTGTAAAAAGCGCTGCCGATTTAGACAATGCATGGACCTATTCTCAAGTTGGCGG
TCGTAGTGGTGAAGGTCGTATCATTATTGAGGGTTTTGTGCCCTTTGATTATGAAATCACGCTATTAACAGTGAATGCCG
TTGATGGTACTCATTTCTGTGACCCTATTGGTCATCGTCAGGAAAATGGTGATTACCGCGAATCCTGGCAACCACAAGCA
ATGTCAACGGTGGCGCTGCAGAAGGCACAGAATATCGCCAAAAAAATAGTCACTGAACTAGGTGGATATGGTTTATTTGG
TATGGAGTTTTTTGTCAAAGGCGATCAGGTTTATTTTAGCGAAGTCTCCCCTCGCCCGCACGATACCGGATTAGTTACCT
TAGTTTCACAGGATGCTTCAGAATTTGCGTTACATGTTCGTGCCATTTTAGGTTTTCCTATTGGTCGTATTATTCAATAT
GGCCCCTGCGCATCGAGTGTTATTTTAGGCAATGGTCTATCCGATGATATTAAATTTACCAACCTAGATCAAGCACTCGG
CAGCGTCGCCGGTGCGCAAATTCGACTATTTGCCAAACCAGATATCAATGGTCAGCGACGTTTAGGTGTCGCCATTGCAC
GTGGTGAAACAGTTGAAGAAGCCGTTGATAATGCCAAGACTGTCTCGAATAAAGTTAATATTATTTATTAA

Upstream 100 bases:

>100_bases
AAAAATATCGAAGGTTTTTTATCTTTGTTCCTCAAAAAATGAAAAAATCTGTTAATATCTTCGCTTACAATCCCTTAAAA
TTTTCAATGAGTCGAATAAT

Downstream 100 bases:

>100_bases
GTAACGCTGCTAAAATTTTGTTGTCAGTATTTAAAGGTAACTTTAGTTACCTTTTTTTATGTCTTTTTTCTCTCCAAACA
CAGCCAGCGCTTAAAAAAAA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSVSIFGSAKANSATKALLLGSGELGKEVAIELQRFGIEVIAADSYENAPAMQVAHSSHVVSMLDGEKLATIIRAEKPDF
IIPEVEAIATDTLLALEAEGFNVVPTARAAKLTMDREGIRRLAAETLGIKTSPYIFADSKEEYLSAIEKIGKPCVIKPVM
SSSGKGQSIVKSAADLDNAWTYSQVGGRSGEGRIIIEGFVPFDYEITLLTVNAVDGTHFCDPIGHRQENGDYRESWQPQA
MSTVALQKAQNIAKKIVTELGGYGLFGMEFFVKGDQVYFSEVSPRPHDTGLVTLVSQDASEFALHVRAILGFPIGRIIQY
GPCASSVILGNGLSDDIKFTNLDQALGSVAGAQIRLFAKPDINGQRRLGVAIARGETVEEAVDNAKTVSNKVNIIY

Sequences:

>Translated_396_residues
MSVSIFGSAKANSATKALLLGSGELGKEVAIELQRFGIEVIAADSYENAPAMQVAHSSHVVSMLDGEKLATIIRAEKPDF
IIPEVEAIATDTLLALEAEGFNVVPTARAAKLTMDREGIRRLAAETLGIKTSPYIFADSKEEYLSAIEKIGKPCVIKPVM
SSSGKGQSIVKSAADLDNAWTYSQVGGRSGEGRIIIEGFVPFDYEITLLTVNAVDGTHFCDPIGHRQENGDYRESWQPQA
MSTVALQKAQNIAKKIVTELGGYGLFGMEFFVKGDQVYFSEVSPRPHDTGLVTLVSQDASEFALHVRAILGFPIGRIIQY
GPCASSVILGNGLSDDIKFTNLDQALGSVAGAQIRLFAKPDINGQRRLGVAIARGETVEEAVDNAKTVSNKVNIIY
>Mature_395_residues
SVSIFGSAKANSATKALLLGSGELGKEVAIELQRFGIEVIAADSYENAPAMQVAHSSHVVSMLDGEKLATIIRAEKPDFI
IPEVEAIATDTLLALEAEGFNVVPTARAAKLTMDREGIRRLAAETLGIKTSPYIFADSKEEYLSAIEKIGKPCVIKPVMS
SSGKGQSIVKSAADLDNAWTYSQVGGRSGEGRIIIEGFVPFDYEITLLTVNAVDGTHFCDPIGHRQENGDYRESWQPQAM
STVALQKAQNIAKKIVTELGGYGLFGMEFFVKGDQVYFSEVSPRPHDTGLVTLVSQDASEFALHVRAILGFPIGRIIQYG
PCASSVILGNGLSDDIKFTNLDQALGSVAGAQIRLFAKPDINGQRRLGVAIARGETVEEAVDNAKTVSNKVNIIY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=62.7551020408163, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1786733, Length=214, Percent_Identity=23.3644859813084, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=369, Percent_Identity=25.2032520325203, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PSYIN (A1SWW0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943574.1
- ProteinModelPortal:   A1SWW0
- SMR:   A1SWW0
- STRING:   A1SWW0
- GeneID:   4624412
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2234
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   A1SWW0
- BioCyc:   PING357804:PING_2234-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42513; Mature: 42382

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 84-84 BINDING 116-116 BINDING 157-157 BINDING 205-205 BINDING 269-269 BINDING 281-281 BINDING 288-288 BINDING 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIFGSAKANSATKALLLGSGELGKEVAIELQRFGIEVIAADSYENAPAMQVAHSSHV
CEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHEEECCCCE
VSMLDGEKLATIIRAEKPDFIIPEVEAIATDTLLALEAEGFNVVPTARAAKLTMDREGIR
EEECCCHHHHHHHCCCCCCEECCCHHHHHCCEEEEEECCCCEECCCCCCEEEEECHHHHH
RLAAETLGIKTSPYIFADSKEEYLSAIEKIGKPCVIKPVMSSSGKGQSIVKSAADLDNAW
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHCCCCE
TYSQVGGRSGEGRIIIEGFVPFDYEITLLTVNAVDGTHFCDPIGHRQENGDYRESWQPQA
EHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHH
MSTVALQKAQNIAKKIVTELGGYGLFGMEFFVKGDQVYFSEVSPRPHDTGLVTLVSQDAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCHH
EFALHVRAILGFPIGRIIQYGPCASSVILGNGLSDDIKFTNLDQALGSVAGAQIRLFAKP
HHHHHHHHHHCCCHHHHEECCCCCCCEEECCCCCCCCEECCHHHHHHHCCCCEEEEEECC
DINGQRRLGVAIARGETVEEAVDNAKTVSNKVNIIY
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
SVSIFGSAKANSATKALLLGSGELGKEVAIELQRFGIEVIAADSYENAPAMQVAHSSHV
EEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHEEECCCCE
VSMLDGEKLATIIRAEKPDFIIPEVEAIATDTLLALEAEGFNVVPTARAAKLTMDREGIR
EEECCCHHHHHHHCCCCCCEECCCHHHHHCCEEEEEECCCCEECCCCCCEEEEECHHHHH
RLAAETLGIKTSPYIFADSKEEYLSAIEKIGKPCVIKPVMSSSGKGQSIVKSAADLDNAW
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHCCCCE
TYSQVGGRSGEGRIIIEGFVPFDYEITLLTVNAVDGTHFCDPIGHRQENGDYRESWQPQA
EHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHH
MSTVALQKAQNIAKKIVTELGGYGLFGMEFFVKGDQVYFSEVSPRPHDTGLVTLVSQDAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCHH
EFALHVRAILGFPIGRIIQYGPCASSVILGNGLSDDIKFTNLDQALGSVAGAQIRLFAKP
HHHHHHHHHHCCCHHHHEECCCCCCCEEECCCCCCCCEECCHHHHHHHCCCCEEEEEECC
DINGQRRLGVAIARGETVEEAVDNAKTVSNKVNIIY
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA