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The map label for this gene is queA [H]

Identifier: 119945875

GI number: 119945875

Start: 2697979

End: 2699034

Strand: Reverse

Name: queA [H]

Synonym: Ping_2215

Alternate gene names: 119945875

Gene position: 2699034-2697979 (Counterclockwise)

Preceding gene: 119945876

Following gene: 119945874

Centisome position: 59.19

GC content: 39.77

Gene sequence:

>1056_bases
ATGTTTTGCATGAAAGTTTCAGATTTCTCTTTTGAATTACCGCCAGCACTAATTGCTCGTTACCCTATGAAAGACCGTTC
AGCTAGCCGTTTATTGTCGGTGGAAGGGAATTCTGGTGAGTTAAAACATCTACAATTTACTGATCTGGTCGATCTTATTG
ATGAAAATGATTTACTTATTTTTAATGATACCAGGGTCATTCCTGCCCGTCTTTTTGGTGAAAAAGAGACAGGTGGAAAA
ATAGAAGTGCTGGTTGAAAGAGTCTTAGATGATAAAAGTTTCCTCGCGCATCTGCGCAGTTCTAAATCACCAAAACCAGG
CTGTAAATTACGTTTAGAAAATAGCATTGATGCTGTGATGCTAAAACGTCAGGGACCCTTATTTGAAATACATATTGATC
ATGGCAGCACAGTACTTGAAGTCCTTGAGTCAATTGGTCATATGCCATTGCCTCCCTATATTGACCGCCCTGATGAAGAT
GCCGATAAAGAGCGTTACCAGACTGTTTATAATAAAAACCCGGGGGCGGTTGCCGCACCCACTGCAGGTCTTCATTTTGA
TCAGGCAGTTATGACAAAATTGCAGGATAAAGGCGTTGATTTTGCCTTTGTTACACTGCATGTTGGCGCGGGAACATTTC
AACCTGTCAAAGTAGATAATATACTCGATCATCAAATGCACAGTGAATATGCCGAGGTGTCACAAGAAGTTATTGATAAA
ATAAACACCACTAAAGCGAAAGGCGGGCGTGTTATTGCCGTTGGTACAACTTCTGTACGTTCCGTTGAAAGCGCAGCACA
AGTGAGTGTTAAAATGGGTAATGACCTTGCCCCATTCTTTTCTGAAACGAGCATTTTTATTTACCCGGGCTATCAATTTC
AGATAATTGATGCACTAGTGACCAATTTTCATTTGTCAGAATCGACACTATTGATGCTGGTGTCCGCTTTTTCGGGTAAA
GAAAATATTGATAATGCCTACAAAGTGGCTATTCAGGAAAAATATCGTTTTTTTAGTTATGGTGATGCGATGTTTTTAAC
TAAAAAAACCAAATAG

Upstream 100 bases:

>100_bases
TTATAATTAACTATTTTATGGCATCATTTTTCTTTTTTAAGTTACTCCCGCTTGACTTCACTTTTGTCATCCTTAAACTA
CATCGATTTATCCTTCCGGA

Downstream 100 bases:

>100_bases
ATAACAGTCAGTCGTTATATAAGCAAAACAGTCGTTGTTCCTGTTTTGAATTATTAACTGCTTTAAATATATAAAATAGA
TAAAACAAACAAAAATATAA

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MFCMKVSDFSFELPPALIARYPMKDRSASRLLSVEGNSGELKHLQFTDLVDLIDENDLLIFNDTRVIPARLFGEKETGGK
IEVLVERVLDDKSFLAHLRSSKSPKPGCKLRLENSIDAVMLKRQGPLFEIHIDHGSTVLEVLESIGHMPLPPYIDRPDED
ADKERYQTVYNKNPGAVAAPTAGLHFDQAVMTKLQDKGVDFAFVTLHVGAGTFQPVKVDNILDHQMHSEYAEVSQEVIDK
INTTKAKGGRVIAVGTTSVRSVESAAQVSVKMGNDLAPFFSETSIFIYPGYQFQIIDALVTNFHLSESTLLMLVSAFSGK
ENIDNAYKVAIQEKYRFFSYGDAMFLTKKTK

Sequences:

>Translated_351_residues
MFCMKVSDFSFELPPALIARYPMKDRSASRLLSVEGNSGELKHLQFTDLVDLIDENDLLIFNDTRVIPARLFGEKETGGK
IEVLVERVLDDKSFLAHLRSSKSPKPGCKLRLENSIDAVMLKRQGPLFEIHIDHGSTVLEVLESIGHMPLPPYIDRPDED
ADKERYQTVYNKNPGAVAAPTAGLHFDQAVMTKLQDKGVDFAFVTLHVGAGTFQPVKVDNILDHQMHSEYAEVSQEVIDK
INTTKAKGGRVIAVGTTSVRSVESAAQVSVKMGNDLAPFFSETSIFIYPGYQFQIIDALVTNFHLSESTLLMLVSAFSGK
ENIDNAYKVAIQEKYRFFSYGDAMFLTKKTK
>Mature_351_residues
MFCMKVSDFSFELPPALIARYPMKDRSASRLLSVEGNSGELKHLQFTDLVDLIDENDLLIFNDTRVIPARLFGEKETGGK
IEVLVERVLDDKSFLAHLRSSKSPKPGCKLRLENSIDAVMLKRQGPLFEIHIDHGSTVLEVLESIGHMPLPPYIDRPDED
ADKERYQTVYNKNPGAVAAPTAGLHFDQAVMTKLQDKGVDFAFVTLHVGAGTFQPVKVDNILDHQMHSEYAEVSQEVIDK
INTTKAKGGRVIAVGTTSVRSVESAAQVSVKMGNDLAPFFSETSIFIYPGYQFQIIDALVTNFHLSESTLLMLVSAFSGK
ENIDNAYKVAIQEKYRFFSYGDAMFLTKKTK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=66.8587896253602, Blast_Score=482, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39131; Mature: 39131

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCMKVSDFSFELPPALIARYPMKDRSASRLLSVEGNSGELKHLQFTDLVDLIDENDLLI
CEEEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCEEE
FNDTRVIPARLFGEKETGGKIEVLVERVLDDKSFLAHLRSSKSPKPGCKLRLENSIDAVM
EECCEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCEEE
LKRQGPLFEIHIDHGSTVLEVLESIGHMPLPPYIDRPDEDADKERYQTVYNKNPGAVAAP
EECCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECC
TAGLHFDQAVMTKLQDKGVDFAFVTLHVGAGTFQPVKVDNILDHQMHSEYAEVSQEVIDK
CCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
INTTKAKGGRVIAVGTTSVRSVESAAQVSVKMGNDLAPFFSETSIFIYPGYQFQIIDALV
HCCCCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCEEEEHHHHH
TNFHLSESTLLMLVSAFSGKENIDNAYKVAIQEKYRFFSYGDAMFLTKKTK
HHCCCCCHHHHHHHHHHCCCCCCCHHHEEHHHHHEEEEECCCEEEEEECCC
>Mature Secondary Structure
MFCMKVSDFSFELPPALIARYPMKDRSASRLLSVEGNSGELKHLQFTDLVDLIDENDLLI
CEEEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCEEE
FNDTRVIPARLFGEKETGGKIEVLVERVLDDKSFLAHLRSSKSPKPGCKLRLENSIDAVM
EECCEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCEEE
LKRQGPLFEIHIDHGSTVLEVLESIGHMPLPPYIDRPDEDADKERYQTVYNKNPGAVAAP
EECCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECC
TAGLHFDQAVMTKLQDKGVDFAFVTLHVGAGTFQPVKVDNILDHQMHSEYAEVSQEVIDK
CCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
INTTKAKGGRVIAVGTTSVRSVESAAQVSVKMGNDLAPFFSETSIFIYPGYQFQIIDALV
HCCCCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCEEEEHHHHH
TNFHLSESTLLMLVSAFSGKENIDNAYKVAIQEKYRFFSYGDAMFLTKKTK
HHCCCCCHHHHHHHHHHCCCCCCCHHHEEHHHHHEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA