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The map label for this gene is tgt

Identifier: 119945874

GI number: 119945874

Start: 2696726

End: 2697850

Strand: Reverse

Name: tgt

Synonym: Ping_2214

Alternate gene names: 119945874

Gene position: 2697850-2696726 (Counterclockwise)

Preceding gene: 119945875

Following gene: 119945873

Centisome position: 59.17

GC content: 41.69

Gene sequence:

>1125_bases
ATGGAATATAAATTAATTAATACTGATGGGCGTGCGCGTCGGGGCCGTTTGACCTTTGATCGTGGCAGCGTTGAAACTCC
CGCATTTATGCCTGTGGGTACTTACGGTACAGTAAAAGGAATGACGCCCGAAGAGGTTGATGCAACCGGCGCAGAAATTT
TATTGGGAAATACCTTTCACTTGTGGTTACGCCCCGGTCAGAAAGTCATCAAAGCACACGGTGATTTGCATGATTTTATG
AATTGGAAGGGCCCCATTTTAACAGATTCAGGCGGCTTTCAAGTATTTAGCTTAGGTAAAATGCGCAAGATAAAAGAAGA
GGGGGTTTATTTTCGTAGCCCTATTAATGGTAGTGAGGTATTTTTATCACCTGAAATATCGATGGATATACAGTACGATT
TAGGATCTGACATTGTGATGATCTTTGATGAATGTACTCCTTATCCGGCGACAGAAGAAGAAACGGATGTTTCGATGCAA
CTTTCCCTACGCTGGGCGCAGCGCAGTCGGGATCGTTTTGATGAACAACAAAATCCGAATGCTTTATTTGGTATTATTCA
GGGGGGGTGTTTCGAGCAATTTCGCGATATTTCTTTAGATGGATTAACCAATATAGGTTTTGATGGTTATGCGATTGGTG
GCCTTGCAGTGGGTGAGCCGAAAGAAGATATGTACCGCATCTTAGAATATATCGCGCCTAAAATCCCTGAGGATAAACCC
CGTTATTTAATGGGGGTTGGCAAACCTGAAGATTTAGTTGAAGGCGTGCGTCGCGGTATTGATATGTTTGATTGTGTTAT
GCCGACTCGCAATGCGCGTAATGGGCATTTATTCACAACGGATGGTGTGATAAAAATTCGTAACGCTAAACACCGTGAAG
ATGCAACTACTTTAGATTCCGAATGTGATTGTTACACCTGTAAAAACTATACGCGTGCATATCTATATCATCTTGATAAA
TGTGGAGAAATACTGGGTGCGCGATTAAACACTATCCATAACTTAAGTTACTATCAACGTTTAATGAAAGGTTTGCGTGA
AGCCATTGAACAGGGTAAATTAGAAGACTTTGTTGATACGTTTTATCAACGCATCGGAAAAGAAAAACCGATGCTGGACG
TTTAA

Upstream 100 bases:

>100_bases
CAGTCGTTGTTCCTGTTTTGAATTATTAACTGCTTTAAATATATAAAATAGATAAAACAAACAAAAATATAATCAGATTG
TTTATCTGATAAGGAAAATC

Downstream 100 bases:

>100_bases
CAAATTTAGCTATAACAATAATATTTAATTTTAATTTATAAGGGTAACAAAATGAGTTTATTTATTTCTGAAGCACATGC
AGCAGGTGAAGGTCAAGCAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MEYKLINTDGRARRGRLTFDRGSVETPAFMPVGTYGTVKGMTPEEVDATGAEILLGNTFHLWLRPGQKVIKAHGDLHDFM
NWKGPILTDSGGFQVFSLGKMRKIKEEGVYFRSPINGSEVFLSPEISMDIQYDLGSDIVMIFDECTPYPATEEETDVSMQ
LSLRWAQRSRDRFDEQQNPNALFGIIQGGCFEQFRDISLDGLTNIGFDGYAIGGLAVGEPKEDMYRILEYIAPKIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFTTDGVIKIRNAKHREDATTLDSECDCYTCKNYTRAYLYHLDK
CGEILGARLNTIHNLSYYQRLMKGLREAIEQGKLEDFVDTFYQRIGKEKPMLDV

Sequences:

>Translated_374_residues
MEYKLINTDGRARRGRLTFDRGSVETPAFMPVGTYGTVKGMTPEEVDATGAEILLGNTFHLWLRPGQKVIKAHGDLHDFM
NWKGPILTDSGGFQVFSLGKMRKIKEEGVYFRSPINGSEVFLSPEISMDIQYDLGSDIVMIFDECTPYPATEEETDVSMQ
LSLRWAQRSRDRFDEQQNPNALFGIIQGGCFEQFRDISLDGLTNIGFDGYAIGGLAVGEPKEDMYRILEYIAPKIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFTTDGVIKIRNAKHREDATTLDSECDCYTCKNYTRAYLYHLDK
CGEILGARLNTIHNLSYYQRLMKGLREAIEQGKLEDFVDTFYQRIGKEKPMLDV
>Mature_374_residues
MEYKLINTDGRARRGRLTFDRGSVETPAFMPVGTYGTVKGMTPEEVDATGAEILLGNTFHLWLRPGQKVIKAHGDLHDFM
NWKGPILTDSGGFQVFSLGKMRKIKEEGVYFRSPINGSEVFLSPEISMDIQYDLGSDIVMIFDECTPYPATEEETDVSMQ
LSLRWAQRSRDRFDEQQNPNALFGIIQGGCFEQFRDISLDGLTNIGFDGYAIGGLAVGEPKEDMYRILEYIAPKIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFTTDGVIKIRNAKHREDATTLDSECDCYTCKNYTRAYLYHLDK
CGEILGARLNTIHNLSYYQRLMKGLREAIEQGKLEDFVDTFYQRIGKEKPMLDV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=354, Percent_Identity=44.3502824858757, Blast_Score=301, Evalue=7e-82,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=77.0053475935829, Blast_Score=617, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=40.8, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=195, Percent_Identity=30.2564102564103, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920510, Length=376, Percent_Identity=42.2872340425532, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI21355311, Length=173, Percent_Identity=31.2138728323699, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_PSYIN (A1SWU0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943554.1
- ProteinModelPortal:   A1SWU0
- SMR:   A1SWU0
- STRING:   A1SWU0
- GeneID:   4623851
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2214
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   A1SWU0
- BioCyc:   PING357804:PING_2214-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42489; Mature: 42489

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKLINTDGRARRGRLTFDRGSVETPAFMPVGTYGTVKGMTPEEVDATGAEILLGNTFH
CCEEEECCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCEEEECCEEE
LWLRPGQKVIKAHGDLHDFMNWKGPILTDSGGFQVFSLGKMRKIKEEGVYFRSPINGSEV
EEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCCCEE
FLSPEISMDIQYDLGSDIVMIFDECTPYPATEEETDVSMQLSLRWAQRSRDRFDEQQNPN
EECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCC
ALFGIIQGGCFEQFRDISLDGLTNIGFDGYAIGGLAVGEPKEDMYRILEYIAPKIPEDKP
EEEEECCCCHHHHHHCCCCCCCCCCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFTTDGVIKIRNAKHREDATTLDS
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCC
ECDCYTCKNYTRAYLYHLDKCGEILGARLNTIHNLSYYQRLMKGLREAIEQGKLEDFVDT
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FYQRIGKEKPMLDV
HHHHHCCCCCCCCC
>Mature Secondary Structure
MEYKLINTDGRARRGRLTFDRGSVETPAFMPVGTYGTVKGMTPEEVDATGAEILLGNTFH
CCEEEECCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCEEEECCEEE
LWLRPGQKVIKAHGDLHDFMNWKGPILTDSGGFQVFSLGKMRKIKEEGVYFRSPINGSEV
EEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCCCEE
FLSPEISMDIQYDLGSDIVMIFDECTPYPATEEETDVSMQLSLRWAQRSRDRFDEQQNPN
EECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCC
ALFGIIQGGCFEQFRDISLDGLTNIGFDGYAIGGLAVGEPKEDMYRILEYIAPKIPEDKP
EEEEECCCCHHHHHHCCCCCCCCCCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFTTDGVIKIRNAKHREDATTLDS
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCC
ECDCYTCKNYTRAYLYHLDKCGEILGARLNTIHNLSYYQRLMKGLREAIEQGKLEDFVDT
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FYQRIGKEKPMLDV
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA