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The map label for this gene is tpiA

Identifier: 119945859

GI number: 119945859

Start: 2675712

End: 2676464

Strand: Direct

Name: tpiA

Synonym: Ping_2199

Alternate gene names: 119945859

Gene position: 2675712-2676464 (Clockwise)

Preceding gene: 119945858

Following gene: 119945860

Centisome position: 58.68

GC content: 42.23

Gene sequence:

>753_bases
ATGAGAACACCACTAGTAATGGGTAACTGGAAATTAAATGGCACAAAAGAATCTGTATCCGCTTTAATCAAAGGCATTGA
AGCCGCAGCAGATGCCGCAAAAAATGTTGAAGTTGCCGTTTGCCCGCCCACTATTTTTATCGAACAAGTAGCAAACCTAG
TTGCAAATAACAGCATCGAATTAGGTGCTCAAGATGTAAGCACAAATATTTCAGGTGCTTTTACAGGTGAGACTTCTCCT
GTAATGGTTAAAGAATTCGGTGCAAAATACAGCTTAGTCGGTCACAGTGAGCGTCGTCAGTACCACAATGAAACCGATGC
CGTTGTAGCTGCAAAATTTGTTGCAATCCAGGCAAATGGTTTAGTCCCTGTATTGTGTATTGGTGAAACGCTAGAAGAAC
GTGAAACTGATAAAACATTTAATGTTGTAGAAACACAGCTTAAAGCCGTTATCGATGTTTCAGGTATTGACGCATTAGAA
AACGCCGTCATTGCATACGAGCCAGTTTGGGCTATCGGCACAGGCAAAGTTGCATCTTCAGAGCAAGCTCAAGATGTTCA
CGCGCACATCCGTACTTGGTTAGCAGAGCAAAGCAAAGTTGTTGCTGCAAAAGTACAAATCCTTTACGGCGGCAGTGTTA
AAGCTTCAAGTGCAAAAGAATTATTTGCCCAGCCTGACATTGATGGTGGTTTAGTGGGTGGCGCAGCATTATTGGTTGAA
GAGTTTGTTGGTATTATAGAAGGTGCTAAATAG

Upstream 100 bases:

>100_bases
GTAGGCTTAACGCAATAGTCATTTATAATCGAATCCGAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTAATTTTA
TAACAATAATGGAGAAAAGT

Downstream 100 bases:

>100_bases
CCTTTTAATTTAATATCCCTATTTAATATCTATAGTTAATACCAATAAAAGGCGCTATTCAGCGCCTTTTTAGTTTCTTG
TCTGACTTTCCCGCATAACC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRTPLVMGNWKLNGTKESVSALIKGIEAAADAAKNVEVAVCPPTIFIEQVANLVANNSIELGAQDVSTNISGAFTGETSP
VMVKEFGAKYSLVGHSERRQYHNETDAVVAAKFVAIQANGLVPVLCIGETLEERETDKTFNVVETQLKAVIDVSGIDALE
NAVIAYEPVWAIGTGKVASSEQAQDVHAHIRTWLAEQSKVVAAKVQILYGGSVKASSAKELFAQPDIDGGLVGGAALLVE
EFVGIIEGAK

Sequences:

>Translated_250_residues
MRTPLVMGNWKLNGTKESVSALIKGIEAAADAAKNVEVAVCPPTIFIEQVANLVANNSIELGAQDVSTNISGAFTGETSP
VMVKEFGAKYSLVGHSERRQYHNETDAVVAAKFVAIQANGLVPVLCIGETLEERETDKTFNVVETQLKAVIDVSGIDALE
NAVIAYEPVWAIGTGKVASSEQAQDVHAHIRTWLAEQSKVVAAKVQILYGGSVKASSAKELFAQPDIDGGLVGGAALLVE
EFVGIIEGAK
>Mature_250_residues
MRTPLVMGNWKLNGTKESVSALIKGIEAAADAAKNVEVAVCPPTIFIEQVANLVANNSIELGAQDVSTNISGAFTGETSP
VMVKEFGAKYSLVGHSERRQYHNETDAVVAAKFVAIQANGLVPVLCIGETLEERETDKTFNVVETQLKAVIDVSGIDALE
NAVIAYEPVWAIGTGKVASSEQAQDVHAHIRTWLAEQSKVVAAKVQILYGGSVKASSAKELFAQPDIDGGLVGGAALLVE
EFVGIIEGAK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=45.9349593495935, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=45.9349593495935, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=51.6, Blast_Score=256, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=47.5609756097561, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=45.9349593495935, Blast_Score=206, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=47.9674796747967, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=47.9674796747967, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=47.9674796747967, Blast_Score=204, Evalue=4e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PSYIN (A1SWS5)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943539.1
- ProteinModelPortal:   A1SWS5
- SMR:   A1SWS5
- STRING:   A1SWS5
- GeneID:   4625233
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2199
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   A1SWS5
- ProtClustDB:   PRK00042
- BioCyc:   PING357804:PING_2199-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26360; Mature: 26360

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPLVMGNWKLNGTKESVSALIKGIEAAADAAKNVEVAVCPPTIFIEQVANLVANNSIE
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCE
LGAQDVSTNISGAFTGETSPVMVKEFGAKYSLVGHSERRQYHNETDAVVAAKFVAIQANG
ECCHHHCCCCCEEECCCCCCCHHHHCCCEEEEECCHHHHHHCCCCHHHHEEEEEEEECCC
LVPVLCIGETLEERETDKTFNVVETQLKAVIDVSGIDALENAVIAYEPVWAIGTGKVASS
CEEEEEECCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCEEEEECCCCCCC
EQAQDVHAHIRTWLAEQSKVVAAKVQILYGGSVKASSAKELFAQPDIDGGLVGGAALLVE
HHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHH
EFVGIIEGAK
HHHHHHCCCC
>Mature Secondary Structure
MRTPLVMGNWKLNGTKESVSALIKGIEAAADAAKNVEVAVCPPTIFIEQVANLVANNSIE
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCE
LGAQDVSTNISGAFTGETSPVMVKEFGAKYSLVGHSERRQYHNETDAVVAAKFVAIQANG
ECCHHHCCCCCEEECCCCCCCHHHHCCCEEEEECCHHHHHHCCCCHHHHEEEEEEEECCC
LVPVLCIGETLEERETDKTFNVVETQLKAVIDVSGIDALENAVIAYEPVWAIGTGKVASS
CEEEEEECCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCEEEEECCCCCCC
EQAQDVHAHIRTWLAEQSKVVAAKVQILYGGSVKASSAKELFAQPDIDGGLVGGAALLVE
HHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHH
EFVGIIEGAK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA