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The map label for this gene is dagA [H]

Identifier: 119945752

GI number: 119945752

Start: 2532237

End: 2533898

Strand: Direct

Name: dagA [H]

Synonym: Ping_2083

Alternate gene names: 119945752

Gene position: 2532237-2533898 (Clockwise)

Preceding gene: 119945750

Following gene: 119945753

Centisome position: 55.54

GC content: 42.48

Gene sequence:

>1662_bases
ATGCCAAGCAGCATTTTATTTCTTCTTTGTGTTTATCTACCCCAGTCATTTGCTAAGGAAAGTACAATGAAGATCAAAAA
GTCAATATTTTCACTCGTTATATTTATAACAACCCTGCTCCAAAGCCAAGCTGTATTCGCTGCTGGCAGCATTGGTACAG
CCATTAATGATACTTTTGCAAATGCAACAGGCTGGTTTGTAAGCCTTATTTTTGCACCTATACCAGGCACCAGTTTTCCC
TGGATAGTCACTTGGCTGGTCATTGCAGCCGTTGTTTTTACCTTATATTTTAGATTTATTCAATTTCGTGCCATCAGACA
TGCGATTGATTTAATTAAGGGAGACTATTCCGATCCCAAAAATCCAGGTGAAGTGACCCACTTTCAGGCGTTAACAACCG
CGCTTTCGGGTACCGTTGGCCTTGGTAATATTGCAGGCGTCGCTGTCGCACTGAGTATTGGTGGACCGGGGGCAACATTC
TGGATGGTGCTTGCGGGACTGTTAGGTATGGCATCTAAATTTACTGAATGTACGCTAGGTGTAAAATATCGTAAAGTAAA
TGCTGATGGCAGTATTTCTGGTGGCCCAATGCATTACATCTCTAAAGGTTTTGCTGAAAGAGGGATTCCTGGAGGCAAAA
TAATGGCCAGTATTTTTGCTATATTTTGTGTACTGAGTTCATTTGGGGCGGGTAATATGTTTCAGGCAAACCAGGCTCAT
GCTCAGATAAGTAATATTACCGGTGCATATCCAGGCTGGATCACAGGTGTTATCTTTGCCGTTCTTATTTTTGCAGTTAT
TGTTGGCGGCATAAAATCTATCGCCAACGTCAGTGAGAAAGTGGTTCCTTTTATGGCCATTCTTTATGTTACGACGGCGC
TGATTATTTTAGGCGCAAATGCGGATCAAATTGGCTGGGCATTTACTCAAATTTTTGAGGGTGCTTTTACTGAAGTAGGT
TTGGCAGGCGGGATTGTTGGAGCCTTAATTCAAGGTTTCAAACGTGCTGCTTTTTCTAACGAAGCGGGCGTCGGCTCGGC
GCCTATCGCTCACTCTGCGGTAAAAACTAATGAGCCAATTACTGAAGGCCTTGTTTCATTGCTTGAACCTTTTATCGATA
CCGTGGTTATCTGTACTATGACGGCATTGGTTATTATTATTTCCGGTCAATTACTTGTTGATGCTAATACCGGCCTTTAT
TTATTAGGTGAAAATGGGCAGATTCTGACCGCTTCTGGCGCTTCTGGCGTTGCCTTAACATCGGTTGCTTTTGGTCAAAC
GGCTAGCTGGTTCCCTTACGTGCTGGCAGTAGCTGTTGTCTTATTTGCATTTTCGACCATGCTTAGCTGGTCATACTACG
GGCTAAAAGCGTGGACCTATTTATTTGGTGAGAGCAAAGCGACAGAAATGAGTTATAAAATTTTATATTGTTGCTTAATT
ATAGTGGGTGCTGCAGCAAGCTTAGGCGCTGTGATTGATTTTTCTGATGCCGCTATGTTTGCTATGGCGATCGTCAATAT
TATCGGCTTGTATTTCTTAATGCCGGTAGTAAAAAGAGAATTAAACTCTTATATGTCGCGCCTACACTCAGGAAAAATAA
AGAAATTTGTTGAGGAAGAAGACGACGCTGATATAGCCCTGCCTGCTGAGGACAAAATTTAG

Upstream 100 bases:

>100_bases
CAACATAGCGGTTAATAGCTAACAATAAATTAAAAGGAAATTATTATGCTTATCGGTGTATCTCAAGACATTAAAAATCA
TCAAAATACCCTAGGAAGAA

Downstream 100 bases:

>100_bases
CCAAACAACCAGGTAAATTTAATCGTCGATTAATTTGTTATATTTAATGCGGGTCATCGGGCTCGCATTTTTTTTACCCT
ATAGAAAAAAGATAGGGTAG

Product: amino acid transport integral membrane protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MPSSILFLLCVYLPQSFAKESTMKIKKSIFSLVIFITTLLQSQAVFAAGSIGTAINDTFANATGWFVSLIFAPIPGTSFP
WIVTWLVIAAVVFTLYFRFIQFRAIRHAIDLIKGDYSDPKNPGEVTHFQALTTALSGTVGLGNIAGVAVALSIGGPGATF
WMVLAGLLGMASKFTECTLGVKYRKVNADGSISGGPMHYISKGFAERGIPGGKIMASIFAIFCVLSSFGAGNMFQANQAH
AQISNITGAYPGWITGVIFAVLIFAVIVGGIKSIANVSEKVVPFMAILYVTTALIILGANADQIGWAFTQIFEGAFTEVG
LAGGIVGALIQGFKRAAFSNEAGVGSAPIAHSAVKTNEPITEGLVSLLEPFIDTVVICTMTALVIIISGQLLVDANTGLY
LLGENGQILTASGASGVALTSVAFGQTASWFPYVLAVAVVLFAFSTMLSWSYYGLKAWTYLFGESKATEMSYKILYCCLI
IVGAAASLGAVIDFSDAAMFAMAIVNIIGLYFLMPVVKRELNSYMSRLHSGKIKKFVEEEDDADIALPAEDKI

Sequences:

>Translated_553_residues
MPSSILFLLCVYLPQSFAKESTMKIKKSIFSLVIFITTLLQSQAVFAAGSIGTAINDTFANATGWFVSLIFAPIPGTSFP
WIVTWLVIAAVVFTLYFRFIQFRAIRHAIDLIKGDYSDPKNPGEVTHFQALTTALSGTVGLGNIAGVAVALSIGGPGATF
WMVLAGLLGMASKFTECTLGVKYRKVNADGSISGGPMHYISKGFAERGIPGGKIMASIFAIFCVLSSFGAGNMFQANQAH
AQISNITGAYPGWITGVIFAVLIFAVIVGGIKSIANVSEKVVPFMAILYVTTALIILGANADQIGWAFTQIFEGAFTEVG
LAGGIVGALIQGFKRAAFSNEAGVGSAPIAHSAVKTNEPITEGLVSLLEPFIDTVVICTMTALVIIISGQLLVDANTGLY
LLGENGQILTASGASGVALTSVAFGQTASWFPYVLAVAVVLFAFSTMLSWSYYGLKAWTYLFGESKATEMSYKILYCCLI
IVGAAASLGAVIDFSDAAMFAMAIVNIIGLYFLMPVVKRELNSYMSRLHSGKIKKFVEEEDDADIALPAEDKI
>Mature_552_residues
PSSILFLLCVYLPQSFAKESTMKIKKSIFSLVIFITTLLQSQAVFAAGSIGTAINDTFANATGWFVSLIFAPIPGTSFPW
IVTWLVIAAVVFTLYFRFIQFRAIRHAIDLIKGDYSDPKNPGEVTHFQALTTALSGTVGLGNIAGVAVALSIGGPGATFW
MVLAGLLGMASKFTECTLGVKYRKVNADGSISGGPMHYISKGFAERGIPGGKIMASIFAIFCVLSSFGAGNMFQANQAHA
QISNITGAYPGWITGVIFAVLIFAVIVGGIKSIANVSEKVVPFMAILYVTTALIILGANADQIGWAFTQIFEGAFTEVGL
AGGIVGALIQGFKRAAFSNEAGVGSAPIAHSAVKTNEPITEGLVSLLEPFIDTVVICTMTALVIIISGQLLVDANTGLYL
LGENGQILTASGASGVALTSVAFGQTASWFPYVLAVAVVLFAFSTMLSWSYYGLKAWTYLFGESKATEMSYKILYCCLII
VGAAASLGAVIDFSDAAMFAMAIVNIIGLYFLMPVVKRELNSYMSRLHSGKIKKFVEEEDDADIALPAEDKI

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=452, Percent_Identity=33.4070796460177, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 58620; Mature: 58489

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILFLLCVYLPQSFAKESTMKIKKSIFSLVIFITTLLQSQAVFAAGSIGTAINDTFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
NATGWFVSLIFAPIPGTSFPWIVTWLVIAAVVFTLYFRFIQFRAIRHAIDLIKGDYSDPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NPGEVTHFQALTTALSGTVGLGNIAGVAVALSIGGPGATFWMVLAGLLGMASKFTECTLG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
VKYRKVNADGSISGGPMHYISKGFAERGIPGGKIMASIFAIFCVLSSFGAGNMFQANQAH
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AQISNITGAYPGWITGVIFAVLIFAVIVGGIKSIANVSEKVVPFMAILYVTTALIILGAN
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ADQIGWAFTQIFEGAFTEVGLAGGIVGALIQGFKRAAFSNEAGVGSAPIAHSAVKTNEPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCH
TEGLVSLLEPFIDTVVICTMTALVIIISGQLLVDANTGLYLLGENGQILTASGASGVALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEECCCCCCCHH
SVAFGQTASWFPYVLAVAVVLFAFSTMLSWSYYGLKAWTYLFGESKATEMSYKILYCCLI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IVGAAASLGAVIDFSDAAMFAMAIVNIIGLYFLMPVVKRELNSYMSRLHSGKIKKFVEEE
HHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
DDADIALPAEDKI
CCCCEECCCCCCC
>Mature Secondary Structure 
PSSILFLLCVYLPQSFAKESTMKIKKSIFSLVIFITTLLQSQAVFAAGSIGTAINDTFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
NATGWFVSLIFAPIPGTSFPWIVTWLVIAAVVFTLYFRFIQFRAIRHAIDLIKGDYSDPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NPGEVTHFQALTTALSGTVGLGNIAGVAVALSIGGPGATFWMVLAGLLGMASKFTECTLG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
VKYRKVNADGSISGGPMHYISKGFAERGIPGGKIMASIFAIFCVLSSFGAGNMFQANQAH
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AQISNITGAYPGWITGVIFAVLIFAVIVGGIKSIANVSEKVVPFMAILYVTTALIILGAN
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ADQIGWAFTQIFEGAFTEVGLAGGIVGALIQGFKRAAFSNEAGVGSAPIAHSAVKTNEPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCH
TEGLVSLLEPFIDTVVICTMTALVIIISGQLLVDANTGLYLLGENGQILTASGASGVALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEECCCCCCCHH
SVAFGQTASWFPYVLAVAVVLFAFSTMLSWSYYGLKAWTYLFGESKATEMSYKILYCCLI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IVGAAASLGAVIDFSDAAMFAMAIVNIIGLYFLMPVVKRELNSYMSRLHSGKIKKFVEEE
HHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
DDADIALPAEDKI
CCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]