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The map label for this gene is rafA [H]

Identifier: 119945697

GI number: 119945697

Start: 2450553

End: 2452655

Strand: Direct

Name: rafA [H]

Synonym: Ping_2014

Alternate gene names: 119945697

Gene position: 2450553-2452655 (Clockwise)

Preceding gene: 119945695

Following gene: 119945698

Centisome position: 53.74

GC content: 44.37

Gene sequence:

>2103_bases
ATGAAAAATATCATTCATCTTACCAGCAAAAACTGCAGCTTGATCATCAAGGTTGAAACTACCCCTGAAATTCTTCATTG
GGGGCCAAAAATAAATCAGATTGATCCCGATATTCTGCTCGCTACCGAGCGGCCTATTCCCCAAGCGCGCCTTGATGTTG
ATGTGCCGCTTTCTTTATGTCCTGAGTTAGGCAGTGGTCATTTTAATGCACCCGGTCTGGAAGGTCATCGTGAAAGTTTT
GATTGGGCCCCTGTTTTTGAAGTATTTGATCAGGATTTGGCAGCGCAGTCTGTCGTCTTTAAACTGCAGGATAAGGTTGC
CAAACTGCAGTTAGAGATTGAAATAAAACTGGATTTTGAAAGTGATGTTATCCAAAAACGTATTAAAGTTATTAACACCG
GTGAGACTAAATATTCTTTAACCAAGCTTTCTTCTACCCTGCCCCTGCCAAACCATGCTAATGAACTGATGTCTTTCCAC
GGTCGTTGGAGTCGTGAATTCCAAACCCATCGTCAACGTTTCGCGCACGGTGGTTTTATGCAGGAAAACCGTCGTGGTCG
AACTTCTCACGAAAATTTTCCTGGTCTATTTGTCGGCAGCGATCATTTTAATGAACAAAATGGTCAAGTTTGGGGCTTTC
ATTTAGGTTGGAGTGGTAACCATCAACTGCGTGCGGATGTAAGAAGTGACGGTCGCCGTTTTGTGCAGGCGGGTGAATTA
TTACTCTCTGGAGAAGTTGTTTTAGGGGCATTAGAAAGTTATAGCACCCCCTGGTTATACGCAACTTACAGTGCAGCGGG
TCTTAACGGTATTTCTGAGCACTTCCACCGTTTTGTGCGTGATAATATTATTAAATTCCCAGAGGATAAACCCCGTCCGG
TGCACCTGAATACCTGGGAAGGTATCTATTTCGAGCATGATCCACAATACATTATGAAGATGGCGAATGAAGCCGCTGTA
ATGGGTGTTGAACGCTTTATTATTGATGATGGCTGGTTTATTGGGCGACATGGGGAACGTGCGGCACTCGGTGATTGGTA
CCTTGATAAGGAAAAATATCCAAATGGTTTAGAGCCCGTTATTAAACACGTCAACGATCTGGATATGGAGTTTGGTTTGT
GGGTTGAGCCGGAAATGGTCAGCGAGGAATCAATGCTGTACCGCGCTCATCCGGATTGGGTTTTAGCGTTACAGGGTTAC
CATCAACCTTCTGGTCGCTGGCAATATGTGCTGGATTTACAAAATACAGCATGTTTCAACTACCTGTTTGAACGCTTAAA
CGATCTCTTAACACGTTACAATATCTCTTACCTAAAATGGGATATGAACCGTGAGTTAGTACAACCTGGACATCAGGGTA
AACCGGCGGTCAGTGGGCAGACTAACGCCTTATACGCGTTGCTTGATAAATTATTAGCGGTCCACACGGAAGTTGAAATT
GAATCCTGTTCATCGGGTGGCGGACGCATTGACTTTGAAATTCTCAAACGTACTCATCGTTTCTGGCCATCGGATTGTAA
TGATGCGTTAGAGCGTCAAACTATCCAACGCGGCATGAGCTACTTCTTCCCACCGGAAGTGATGGGCACCCATATCGGCC
CGGAAGAAAGTCATACAACACGCCGTGTTCATCATATCAATATGCGCGGTATGACAGCATTAAGCGGGCACATGGGAGTG
GAATTGGATCCTGTTAAAGTGCCGGTAGAGGAAAGACAAGCCTTTGCTAAATATATTGCACTGCATAAACAATACCGTCA
CTTATTACATAGCGGTCGCAGTTTCCGTGTCGATACTGCAGATAATAGTCAAAATATTTATGGCGTCCATAATAACGATG
AAATGCTAATCACCGTCTGTCAATTAACCATGCCCGATTATGCACTGCCGTCACCACTGCGCATTAGCTGCATCGACACA
ACGGCTCAATATCAAGTTAACTTAGTTGAGACCCCTGAAAACAGCTTCCAATTAATGAAGCAACGCCCTAAATGGTTAGA
TAAAACCCTTACTTTAAGCGGTGAAAGCCTGAAAGAAATCGGGCTGAGCTTGCCTATTCTTGATCCGGAATCAGCGCTTA
TGCTGCACTTGAAAAAACGATAA

Upstream 100 bases:

>100_bases
GATCTATAACACATTTTTTGGCGCATTAATTGTTTAAAAAAGGCAGTACGTGCCAATATCACCAAATATGTTTTCGCAAA
CAAAAAACCTAGAGGATCAA

Downstream 100 bases:

>100_bases
AGCATTTATTGCCGGCTGCCTGATGGGGTAGCCATTTTTTATTGGTCTATTTTGACTATTGTGTTCGTAGCTTATATGCC
TAATTTGTTGCTGCATTTTG

Product: glycoside hydrolase, clan GH-D

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MKNIIHLTSKNCSLIIKVETTPEILHWGPKINQIDPDILLATERPIPQARLDVDVPLSLCPELGSGHFNAPGLEGHRESF
DWAPVFEVFDQDLAAQSVVFKLQDKVAKLQLEIEIKLDFESDVIQKRIKVINTGETKYSLTKLSSTLPLPNHANELMSFH
GRWSREFQTHRQRFAHGGFMQENRRGRTSHENFPGLFVGSDHFNEQNGQVWGFHLGWSGNHQLRADVRSDGRRFVQAGEL
LLSGEVVLGALESYSTPWLYATYSAAGLNGISEHFHRFVRDNIIKFPEDKPRPVHLNTWEGIYFEHDPQYIMKMANEAAV
MGVERFIIDDGWFIGRHGERAALGDWYLDKEKYPNGLEPVIKHVNDLDMEFGLWVEPEMVSEESMLYRAHPDWVLALQGY
HQPSGRWQYVLDLQNTACFNYLFERLNDLLTRYNISYLKWDMNRELVQPGHQGKPAVSGQTNALYALLDKLLAVHTEVEI
ESCSSGGGRIDFEILKRTHRFWPSDCNDALERQTIQRGMSYFFPPEVMGTHIGPEESHTTRRVHHINMRGMTALSGHMGV
ELDPVKVPVEERQAFAKYIALHKQYRHLLHSGRSFRVDTADNSQNIYGVHNNDEMLITVCQLTMPDYALPSPLRISCIDT
TAQYQVNLVETPENSFQLMKQRPKWLDKTLTLSGESLKEIGLSLPILDPESALMLHLKKR

Sequences:

>Translated_700_residues
MKNIIHLTSKNCSLIIKVETTPEILHWGPKINQIDPDILLATERPIPQARLDVDVPLSLCPELGSGHFNAPGLEGHRESF
DWAPVFEVFDQDLAAQSVVFKLQDKVAKLQLEIEIKLDFESDVIQKRIKVINTGETKYSLTKLSSTLPLPNHANELMSFH
GRWSREFQTHRQRFAHGGFMQENRRGRTSHENFPGLFVGSDHFNEQNGQVWGFHLGWSGNHQLRADVRSDGRRFVQAGEL
LLSGEVVLGALESYSTPWLYATYSAAGLNGISEHFHRFVRDNIIKFPEDKPRPVHLNTWEGIYFEHDPQYIMKMANEAAV
MGVERFIIDDGWFIGRHGERAALGDWYLDKEKYPNGLEPVIKHVNDLDMEFGLWVEPEMVSEESMLYRAHPDWVLALQGY
HQPSGRWQYVLDLQNTACFNYLFERLNDLLTRYNISYLKWDMNRELVQPGHQGKPAVSGQTNALYALLDKLLAVHTEVEI
ESCSSGGGRIDFEILKRTHRFWPSDCNDALERQTIQRGMSYFFPPEVMGTHIGPEESHTTRRVHHINMRGMTALSGHMGV
ELDPVKVPVEERQAFAKYIALHKQYRHLLHSGRSFRVDTADNSQNIYGVHNNDEMLITVCQLTMPDYALPSPLRISCIDT
TAQYQVNLVETPENSFQLMKQRPKWLDKTLTLSGESLKEIGLSLPILDPESALMLHLKKR
>Mature_700_residues
MKNIIHLTSKNCSLIIKVETTPEILHWGPKINQIDPDILLATERPIPQARLDVDVPLSLCPELGSGHFNAPGLEGHRESF
DWAPVFEVFDQDLAAQSVVFKLQDKVAKLQLEIEIKLDFESDVIQKRIKVINTGETKYSLTKLSSTLPLPNHANELMSFH
GRWSREFQTHRQRFAHGGFMQENRRGRTSHENFPGLFVGSDHFNEQNGQVWGFHLGWSGNHQLRADVRSDGRRFVQAGEL
LLSGEVVLGALESYSTPWLYATYSAAGLNGISEHFHRFVRDNIIKFPEDKPRPVHLNTWEGIYFEHDPQYIMKMANEAAV
MGVERFIIDDGWFIGRHGERAALGDWYLDKEKYPNGLEPVIKHVNDLDMEFGLWVEPEMVSEESMLYRAHPDWVLALQGY
HQPSGRWQYVLDLQNTACFNYLFERLNDLLTRYNISYLKWDMNRELVQPGHQGKPAVSGQTNALYALLDKLLAVHTEVEI
ESCSSGGGRIDFEILKRTHRFWPSDCNDALERQTIQRGMSYFFPPEVMGTHIGPEESHTTRRVHHINMRGMTALSGHMGV
ELDPVKVPVEERQAFAKYIALHKQYRHLLHSGRSFRVDTADNSQNIYGVHNNDEMLITVCQLTMPDYALPSPLRISCIDT
TAQYQVNLVETPENSFQLMKQRPKWLDKTLTLSGESLKEIGLSLPILDPESALMLHLKKR

Specific function: Unknown

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 80265; Mature: 80265

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIHLTSKNCSLIIKVETTPEILHWGPKINQIDPDILLATERPIPQARLDVDVPLSLC
CCCEEEEECCCCEEEEEEECCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHC
PELGSGHFNAPGLEGHRESFDWAPVFEVFDQDLAAQSVVFKLQDKVAKLQLEIEIKLDFE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCC
SDVIQKRIKVINTGETKYSLTKLSSTLPLPNHANELMSFHGRWSREFQTHRQRFAHGGFM
HHHHHHHHHEEECCCCEEEHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
QENRRGRTSHENFPGLFVGSDHFNEQNGQVWGFHLGWSGNHQLRADVRSDGRRFVQAGEL
CCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCEEEECHHHCHHHHHHHCCE
LLSGEVVLGALESYSTPWLYATYSAAGLNGISEHFHRFVRDNIIKFPEDKPRPVHLNTWE
EEECHHHEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCEEEEECCC
GIYFEHDPQYIMKMANEAAVMGVERFIIDDGWFIGRHGERAALGDWYLDKEKYPNGLEPV
CEEEECCHHHHHHHHHHHHHHEEEEEEEECCEEEECCCCCCCCCCEECCHHCCCCCHHHH
IKHVNDLDMEFGLWVEPEMVSEESMLYRAHPDWVLALQGYHQPSGRWQYVLDLQNTACFN
HHHHHHCCCCCCCEECHHHCCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHH
YLFERLNDLLTRYNISYLKWDMNRELVQPGHQGKPAVSGQTNALYALLDKLLAVHTEVEI
HHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
ESCSSGGGRIDFEILKRTHRFWPSDCNDALERQTIQRGMSYFFPPEVMGTHIGPEESHTT
ECCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
RRVHHINMRGMTALSGHMGVELDPVKVPVEERQAFAKYIALHKQYRHLLHSGRSFRVDTA
EEEEEECCCCCEEECCCCCCEECCEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DNSQNIYGVHNNDEMLITVCQLTMPDYALPSPLRISCIDTTAQYQVNLVETPENSFQLMK
CCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
QRPKWLDKTLTLSGESLKEIGLSLPILDPESALMLHLKKR
HCHHHHCCEEEECCCHHHHCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKNIIHLTSKNCSLIIKVETTPEILHWGPKINQIDPDILLATERPIPQARLDVDVPLSLC
CCCEEEEECCCCEEEEEEECCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHC
PELGSGHFNAPGLEGHRESFDWAPVFEVFDQDLAAQSVVFKLQDKVAKLQLEIEIKLDFE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCC
SDVIQKRIKVINTGETKYSLTKLSSTLPLPNHANELMSFHGRWSREFQTHRQRFAHGGFM
HHHHHHHHHEEECCCCEEEHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
QENRRGRTSHENFPGLFVGSDHFNEQNGQVWGFHLGWSGNHQLRADVRSDGRRFVQAGEL
CCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCEEEECHHHCHHHHHHHCCE
LLSGEVVLGALESYSTPWLYATYSAAGLNGISEHFHRFVRDNIIKFPEDKPRPVHLNTWE
EEECHHHEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCEEEEECCC
GIYFEHDPQYIMKMANEAAVMGVERFIIDDGWFIGRHGERAALGDWYLDKEKYPNGLEPV
CEEEECCHHHHHHHHHHHHHHEEEEEEEECCEEEECCCCCCCCCCEECCHHCCCCCHHHH
IKHVNDLDMEFGLWVEPEMVSEESMLYRAHPDWVLALQGYHQPSGRWQYVLDLQNTACFN
HHHHHHCCCCCCCEECHHHCCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHH
YLFERLNDLLTRYNISYLKWDMNRELVQPGHQGKPAVSGQTNALYALLDKLLAVHTEVEI
HHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
ESCSSGGGRIDFEILKRTHRFWPSDCNDALERQTIQRGMSYFFPPEVMGTHIGPEESHTT
ECCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
RRVHHINMRGMTALSGHMGVELDPVKVPVEERQAFAKYIALHKQYRHLLHSGRSFRVDTA
EEEEEECCCCCEEECCCCCCEECCEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DNSQNIYGVHNNDEMLITVCQLTMPDYALPSPLRISCIDTTAQYQVNLVETPENSFQLMK
CCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
QRPKWLDKTLTLSGESLKEIGLSLPILDPESALMLHLKKR
HCHHHHCCEEEECCCHHHHCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2556373; 2180920; 786627 [H]