Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 119945680

GI number: 119945680

Start: 2426322

End: 2428394

Strand: Direct

Name: mnmC

Synonym: Ping_1996

Alternate gene names: 119945680

Gene position: 2426322-2428394 (Clockwise)

Preceding gene: 119945671

Following gene: 119945681

Centisome position: 53.21

GC content: 40.38

Gene sequence:

>2073_bases
TTGTCTGACGCTAAATACAAGTTAATTAAGCATGCTCAAATACACTGGTCTGATCAGTTAGAGCCTTTTTCGAGTCTATT
CGACGACATTTATTTCAACACAGAGCAAGGAATTGATGAAGCAAATTATGTGTTTTTTGACGGAAATAAGTTAGGAAAAC
GTTGGCTAGATTATTCTGCGCAGCACTTTTCAATAGCCGAAACGGGCTTTGGTACCGGTTTGAATTTTTTGCTTACCTGC
CAGAAATTTGCTGAATTTATAAAATTAAATCCGAATAATCCCCTAAAAAAATTACATTTTAGCAGTTTTGAAAAATATCC
CTTAACTAAGCCGGATTTAATTATTGCATTACAGCAATGGCCGCAATTAACTGCCTTTATTACCCCTCTATTAGCGCAGT
ACCCCATTGCACTAAAGGGCTGCCATCGTTTAGTATTAAATGAGTTTAATATCACCCTCGATTTATGGTTTGGTGATATA
GCAGATACGCTGCCTTCTTTACACGTTTATGATTGCGGACTTTTTGACTGCTGGTATCTGGACGGTTTTGCGCCCAGTAA
AAATCCGGAAATGTGGAATGATGATCTGTTTCAATTAATCAGTCAATCTTGTAAAAAAGATGCCACCATCGCGACCTTTA
CGGCGGCCAGTTTTGTACGCAAAGGATTAATTAAAGCGGGTTTTGAAATGGGAAAACAGAAAGGATATGACAAAAAAAGA
GAGATGTTAATCGGTCATATAAGTACTGAAGCTCTGGCTGCGAACAAACTTAAACAACATGGGCAATATTACCGCCCTGC
AAAATCAGAAAATACTGCACAAAAAAAAACCGATGTTGCCATTATAGGTGGGGGTATTGCAAGTGCTTGTTTATCATTAG
CCCTCATCAGGCGCGGTTATAAAGTCACCTTATACTGCAAAGAGTCCAGCCTGGCCTTTGGCGCATCGGGTAATCAGCAG
GCGGCTTTGTACCCTCTTCTCAATGAACAACACGATGCTTTAAGTCAATTTTTTGCCAATGCATTTTTATACAGCAGAAA
TTATATTAAACAAATAAATCAAACTCAGCCGGTTGATTATAGCTTTTCAGGCGTACTGCAACTCTACTACGATCCCAGCG
CATCCAAAAAACTGGACAAAATTTTACAAGCGGGCTTACCCCAAGAACTGGTTGAACAATTAACAGAAATTGAAACCGAT
AATATTGCCGGGATCTCCATTGCACAAAAATCATTACATTACCCTTTAGGGGGATGGTTAAGTCCCAAGCAACTTGTTGA
AGCTATCGTCCGGCAAGCAAAAAATGAAGGCGACCTGAGCGTACACTTAAATCAAGAATTATTAACCTTTGAGCAAAGCC
CCTCTGACAATCCGGCACTCAATCCGGCACAACCGATAAACAGTTGGGATTTACATTTTGCCAAGCACAGTATTCAACAT
TCACTGCTAGTGCTTGCAACCGCCAATAACACCCTTAATTTTAAACAGTGCGAAGCGGTGCCACTGTCTGCTGCCCGTGG
TCAAGTCACTCATATTCCGACCAACCATAAAATTAATCCATTACAGATAACACTTTGTCATGAAGGATATTTAACCCCGG
CGAATAATCAACAACATTGTATGGGGGCAACCTTCAAACGTCATGATCTTAACCAGGCATTTTCTCAGCAAGAACAAGTC
GATAATAAAGATAAATTAGAAAAATGTATCCCCGGAAAGACCTGGACAGAGCAGATAGACATAAACCACCCAAATGCACA
TGTCGGTATTCGCTGTACAACAAGGGACCATTTCCCCTACGTGGGGGCCATACCCGATTATCAGGCAACTAAAATTCATT
ATAAAAATGCCGCAAAAGAAGTTAACCCGGTCAATGCCCCTTTTCATCCTAATTTATACCTGTTGACCGGATTAGGCTCG
AGAGGTTTTTGCTCGGCACCTCTGTTAGCTGAAATGTTAGCCAGTCAGATAAATAAAGAACCATTGCCCTTTGCAAAGGA
AATCTTAAATGCGATGCAGATTAACAGGCAATGGATAAACTACCTTAAGAAAGGAAAGCCGTTACATTACTAG

Upstream 100 bases:

>100_bases
TGCTAGAGGGACTAATTATATGATGGATCTAAGGAGTGTAATAGATCAATTAAACTCTAATAAATTAAACTAAATTATCG
CATTAAGTGGGAAATATATA

Downstream 100 bases:

>100_bases
TTTGCCTGTATTTTGTGCGTTTTGATTGTTCTCACCGCAAAAAACTTAAAAAGTGCTGATCGCTTTTTATAGAACTTTGT
AAGTTTTCACAATTTACGGT

Product: fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSDAKYKLIKHAQIHWSDQLEPFSSLFDDIYFNTEQGIDEANYVFFDGNKLGKRWLDYSAQHFSIAETGFGTGLNFLLTC
QKFAEFIKLNPNNPLKKLHFSSFEKYPLTKPDLIIALQQWPQLTAFITPLLAQYPIALKGCHRLVLNEFNITLDLWFGDI
ADTLPSLHVYDCGLFDCWYLDGFAPSKNPEMWNDDLFQLISQSCKKDATIATFTAASFVRKGLIKAGFEMGKQKGYDKKR
EMLIGHISTEALAANKLKQHGQYYRPAKSENTAQKKTDVAIIGGGIASACLSLALIRRGYKVTLYCKESSLAFGASGNQQ
AALYPLLNEQHDALSQFFANAFLYSRNYIKQINQTQPVDYSFSGVLQLYYDPSASKKLDKILQAGLPQELVEQLTEIETD
NIAGISIAQKSLHYPLGGWLSPKQLVEAIVRQAKNEGDLSVHLNQELLTFEQSPSDNPALNPAQPINSWDLHFAKHSIQH
SLLVLATANNTLNFKQCEAVPLSAARGQVTHIPTNHKINPLQITLCHEGYLTPANNQQHCMGATFKRHDLNQAFSQQEQV
DNKDKLEKCIPGKTWTEQIDINHPNAHVGIRCTTRDHFPYVGAIPDYQATKIHYKNAAKEVNPVNAPFHPNLYLLTGLGS
RGFCSAPLLAEMLASQINKEPLPFAKEILNAMQINRQWINYLKKGKPLHY

Sequences:

>Translated_690_residues
MSDAKYKLIKHAQIHWSDQLEPFSSLFDDIYFNTEQGIDEANYVFFDGNKLGKRWLDYSAQHFSIAETGFGTGLNFLLTC
QKFAEFIKLNPNNPLKKLHFSSFEKYPLTKPDLIIALQQWPQLTAFITPLLAQYPIALKGCHRLVLNEFNITLDLWFGDI
ADTLPSLHVYDCGLFDCWYLDGFAPSKNPEMWNDDLFQLISQSCKKDATIATFTAASFVRKGLIKAGFEMGKQKGYDKKR
EMLIGHISTEALAANKLKQHGQYYRPAKSENTAQKKTDVAIIGGGIASACLSLALIRRGYKVTLYCKESSLAFGASGNQQ
AALYPLLNEQHDALSQFFANAFLYSRNYIKQINQTQPVDYSFSGVLQLYYDPSASKKLDKILQAGLPQELVEQLTEIETD
NIAGISIAQKSLHYPLGGWLSPKQLVEAIVRQAKNEGDLSVHLNQELLTFEQSPSDNPALNPAQPINSWDLHFAKHSIQH
SLLVLATANNTLNFKQCEAVPLSAARGQVTHIPTNHKINPLQITLCHEGYLTPANNQQHCMGATFKRHDLNQAFSQQEQV
DNKDKLEKCIPGKTWTEQIDINHPNAHVGIRCTTRDHFPYVGAIPDYQATKIHYKNAAKEVNPVNAPFHPNLYLLTGLGS
RGFCSAPLLAEMLASQINKEPLPFAKEILNAMQINRQWINYLKKGKPLHY
>Mature_689_residues
SDAKYKLIKHAQIHWSDQLEPFSSLFDDIYFNTEQGIDEANYVFFDGNKLGKRWLDYSAQHFSIAETGFGTGLNFLLTCQ
KFAEFIKLNPNNPLKKLHFSSFEKYPLTKPDLIIALQQWPQLTAFITPLLAQYPIALKGCHRLVLNEFNITLDLWFGDIA
DTLPSLHVYDCGLFDCWYLDGFAPSKNPEMWNDDLFQLISQSCKKDATIATFTAASFVRKGLIKAGFEMGKQKGYDKKRE
MLIGHISTEALAANKLKQHGQYYRPAKSENTAQKKTDVAIIGGGIASACLSLALIRRGYKVTLYCKESSLAFGASGNQQA
ALYPLLNEQHDALSQFFANAFLYSRNYIKQINQTQPVDYSFSGVLQLYYDPSASKKLDKILQAGLPQELVEQLTEIETDN
IAGISIAQKSLHYPLGGWLSPKQLVEAIVRQAKNEGDLSVHLNQELLTFEQSPSDNPALNPAQPINSWDLHFAKHSIQHS
LLVLATANNTLNFKQCEAVPLSAARGQVTHIPTNHKINPLQITLCHEGYLTPANNQQHCMGATFKRHDLNQAFSQQEQVD
NKDKLEKCIPGKTWTEQIDINHPNAHVGIRCTTRDHFPYVGAIPDYQATKIHYKNAAKEVNPVNAPFHPNLYLLTGLGSR
GFCSAPLLAEMLASQINKEPLPFAKEILNAMQINRQWINYLKKGKPLHY

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=690, Percent_Identity=41.304347826087, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSYIN (A1SW96)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943360.1
- ProteinModelPortal:   A1SW96
- SMR:   A1SW96
- STRING:   A1SW96
- GeneID:   4627202
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1996
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A1SW96
- BioCyc:   PING357804:PING_1996-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 77665; Mature: 77534

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAKYKLIKHAQIHWSDQLEPFSSLFDDIYFNTEQGIDEANYVFFDGNKLGKRWLDYSA
CCCCHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCC
QHFSIAETGFGTGLNFLLTCQKFAEFIKLNPNNPLKKLHFSSFEKYPLTKPDLIIALQQW
CCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHCCCCCCCCCCCCEEEEEHHC
PQLTAFITPLLAQYPIALKGCHRLVLNEFNITLDLWFGDIADTLPSLHVYDCGLFDCWYL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECHHHHHCCCCEEEECCCEEEEEE
DGFAPSKNPEMWNDDLFQLISQSCKKDATIATFTAASFVRKGLIKAGFEMGKQKGYDKKR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EMLIGHISTEALAANKLKQHGQYYRPAKSENTAQKKTDVAIIGGGIASACLSLALIRRGY
HHEEHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCC
KVTLYCKESSLAFGASGNQQAALYPLLNEQHDALSQFFANAFLYSRNYIKQINQTQPVDY
EEEEEEECCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SFSGVLQLYYDPSASKKLDKILQAGLPQELVEQLTEIETDNIAGISIAQKSLHYPLGGWL
CCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC
SPKQLVEAIVRQAKNEGDLSVHLNQELLTFEQSPSDNPALNPAQPINSWDLHFAKHSIQH
CHHHHHHHHHHHCCCCCCEEEEECHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
SLLVLATANNTLNFKQCEAVPLSAARGQVTHIPTNHKINPLQITLCHEGYLTPANNQQHC
EEEEEEECCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCEECCCCCCCHH
MGATFKRHDLNQAFSQQEQVDNKDKLEKCIPGKTWTEQIDINHPNAHVGIRCTTRDHFPY
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCCCCCE
VGAIPDYQATKIHYKNAAKEVNPVNAPFHPNLYLLTGLGSRGFCSAPLLAEMLASQINKE
ECCCCCCCCEEEEHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCC
PLPFAKEILNAMQINRQWINYLKKGKPLHY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDAKYKLIKHAQIHWSDQLEPFSSLFDDIYFNTEQGIDEANYVFFDGNKLGKRWLDYSA
CCCHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCC
QHFSIAETGFGTGLNFLLTCQKFAEFIKLNPNNPLKKLHFSSFEKYPLTKPDLIIALQQW
CCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHCCCCCCCCCCCCEEEEEHHC
PQLTAFITPLLAQYPIALKGCHRLVLNEFNITLDLWFGDIADTLPSLHVYDCGLFDCWYL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECHHHHHCCCCEEEECCCEEEEEE
DGFAPSKNPEMWNDDLFQLISQSCKKDATIATFTAASFVRKGLIKAGFEMGKQKGYDKKR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EMLIGHISTEALAANKLKQHGQYYRPAKSENTAQKKTDVAIIGGGIASACLSLALIRRGY
HHEEHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCC
KVTLYCKESSLAFGASGNQQAALYPLLNEQHDALSQFFANAFLYSRNYIKQINQTQPVDY
EEEEEEECCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SFSGVLQLYYDPSASKKLDKILQAGLPQELVEQLTEIETDNIAGISIAQKSLHYPLGGWL
CCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC
SPKQLVEAIVRQAKNEGDLSVHLNQELLTFEQSPSDNPALNPAQPINSWDLHFAKHSIQH
CHHHHHHHHHHHCCCCCCEEEEECHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
SLLVLATANNTLNFKQCEAVPLSAARGQVTHIPTNHKINPLQITLCHEGYLTPANNQQHC
EEEEEEECCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCEECCCCCCCHH
MGATFKRHDLNQAFSQQEQVDNKDKLEKCIPGKTWTEQIDINHPNAHVGIRCTTRDHFPY
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCCCCCE
VGAIPDYQATKIHYKNAAKEVNPVNAPFHPNLYLLTGLGSRGFCSAPLLAEMLASQINKE
ECCCCCCCCEEEEHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCC
PLPFAKEILNAMQINRQWINYLKKGKPLHY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA