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The map label for this gene is pdxB

Identifier: 119945678

GI number: 119945678

Start: 2423727

End: 2424878

Strand: Reverse

Name: pdxB

Synonym: Ping_1994

Alternate gene names: 119945678

Gene position: 2424878-2423727 (Counterclockwise)

Preceding gene: 119945679

Following gene: 119945677

Centisome position: 53.18

GC content: 40.89

Gene sequence:

>1152_bases
ATGAATATATATATTGATGAAAATATCCCCTACGCGCGGGATTTTTTTACTGGACATGGCAATCTGCATTTTTTTTCGGG
TCGCAATGCCAGCGCAGAACAACTTATTGATGCTGATGTTTTACTGGTTCGTTCGATTACAGAGGTTAATGAAAAATTAC
TCAGCTTAAATAAAAAACTTAAGTTTGTTGGCACTGCAACCATTGGCACAGATCATATCGATCAGACTTACCTCAAAAAC
AGAGGTATTGTTTTTAGCAGTGCGCCAGGCTGTAACAAAGTGTCTGTTGCGGAATATATTTTAAGCAGTTTACTGGTGCT
GGCAGACCAGCAACAATTTATTTTAAGCGATAAAAGTATTGCTATAGTGGGTGCTGGCAATACGGGGACTGCAGTGTACC
AACGCTTAAATGCATTGGGAATGAACTGTAAGCTTTACGATCCACCTCTGCTGTTGGCGGGTGATAAACGGCAATTTTGT
ACCTTTGAGGAGGTACTTGAAGCCGATATCATTAGTTTGCATGTCCCTAAGGTAGTGCAGGGGCCATTTCCAACCGTGCA
TATGTTTGATAGTAAGGTCTTAAGTCAACTTACCAACGGACAAATATTATTAAATGCAGCACGTGGTGATCTTATTGATA
ATCAAGCTTTATTAGAACTTGCCGAGCAGGGTTTAACGCCGACTTTAGTGCTTGATGTCTGGGAAAATGAGCCGCATATT
AATCAACAATTATTACCCTATGTGGCAATCGCAACACCGCATATTGCAGGATACAGCCTGGATGGGCGTGTACGTGGTAC
TGAGATGTTATACCAGGCACTGTGCGATTTTTTGCAGCTAAAGGGAAAATATACCACGGCGGATTTTGTTCACCGTGCCG
CTATCAATAGCGTCTCTATTTCCAAAAAAATCGATCAACCACTGATCAAATCATTAATGCACCTGATATTTGATGTGCGC
CGGGATGATGCCCTGTTCAGAGAAATGATTGAAGATGTTGATGGTTTTGATACTATGCGCAAAACATACCAGGAACGCCG
GGAACTATCGACTTTAACCGTTGAGTCAAGCGTTGAACAAAATACATTATTACAGTTGTTAGGTTTTTCTACCAACGTAC
AAAATGAGATAAAGGAAACACATGAAATCTGA

Upstream 100 bases:

>100_bases
CTGATAAAAATAATAGTTTAATACTGATAAAAAACAAAAAAAGGAGCTTTTAGCTCCTTTTTTTGTCGCAATTTATTCTG
CTATCTTATTAGGAAAAAAA

Downstream 100 bases:

>100_bases
ATATAATATCGCACTGGTGGGTGAACTAAACGGCAGTATCGAAACAACCTTGGAAGTCTTAGCTCAACGTGGCTTTCCCG
TTGCAAAAGTATTTCCTTTG

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNIYIDENIPYARDFFTGHGNLHFFSGRNASAEQLIDADVLLVRSITEVNEKLLSLNKKLKFVGTATIGTDHIDQTYLKN
RGIVFSSAPGCNKVSVAEYILSSLLVLADQQQFILSDKSIAIVGAGNTGTAVYQRLNALGMNCKLYDPPLLLAGDKRQFC
TFEEVLEADIISLHVPKVVQGPFPTVHMFDSKVLSQLTNGQILLNAARGDLIDNQALLELAEQGLTPTLVLDVWENEPHI
NQQLLPYVAIATPHIAGYSLDGRVRGTEMLYQALCDFLQLKGKYTTADFVHRAAINSVSISKKIDQPLIKSLMHLIFDVR
RDDALFREMIEDVDGFDTMRKTYQERRELSTLTVESSVEQNTLLQLLGFSTNVQNEIKETHEI

Sequences:

>Translated_383_residues
MNIYIDENIPYARDFFTGHGNLHFFSGRNASAEQLIDADVLLVRSITEVNEKLLSLNKKLKFVGTATIGTDHIDQTYLKN
RGIVFSSAPGCNKVSVAEYILSSLLVLADQQQFILSDKSIAIVGAGNTGTAVYQRLNALGMNCKLYDPPLLLAGDKRQFC
TFEEVLEADIISLHVPKVVQGPFPTVHMFDSKVLSQLTNGQILLNAARGDLIDNQALLELAEQGLTPTLVLDVWENEPHI
NQQLLPYVAIATPHIAGYSLDGRVRGTEMLYQALCDFLQLKGKYTTADFVHRAAINSVSISKKIDQPLIKSLMHLIFDVR
RDDALFREMIEDVDGFDTMRKTYQERRELSTLTVESSVEQNTLLQLLGFSTNVQNEIKETHEI
>Mature_383_residues
MNIYIDENIPYARDFFTGHGNLHFFSGRNASAEQLIDADVLLVRSITEVNEKLLSLNKKLKFVGTATIGTDHIDQTYLKN
RGIVFSSAPGCNKVSVAEYILSSLLVLADQQQFILSDKSIAIVGAGNTGTAVYQRLNALGMNCKLYDPPLLLAGDKRQFC
TFEEVLEADIISLHVPKVVQGPFPTVHMFDSKVLSQLTNGQILLNAARGDLIDNQALLELAEQGLTPTLVLDVWENEPHI
NQQLLPYVAIATPHIAGYSLDGRVRGTEMLYQALCDFLQLKGKYTTADFVHRAAINSVSISKKIDQPLIKSLMHLIFDVR
RDDALFREMIEDVDGFDTMRKTYQERRELSTLTVESSVEQNTLLQLLGFSTNVQNEIKETHEI

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=305, Percent_Identity=27.8688524590164, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI61743967, Length=293, Percent_Identity=24.5733788395904, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI4557497, Length=293, Percent_Identity=24.5733788395904, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788660, Length=373, Percent_Identity=44.5040214477212, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789279, Length=272, Percent_Identity=28.6764705882353, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082289, Length=276, Percent_Identity=25.3623188405797, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787645, Length=201, Percent_Identity=25.8706467661692, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=27.4900398406374, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=296, Percent_Identity=26.6891891891892, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=259, Percent_Identity=26.6409266409266, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=281, Percent_Identity=27.7580071174377, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=284, Percent_Identity=25.3521126760563, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921140, Length=290, Percent_Identity=29.3103448275862, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24646446, Length=293, Percent_Identity=26.2798634812287, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646448, Length=293, Percent_Identity=26.2798634812287, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646452, Length=293, Percent_Identity=26.2798634812287, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646450, Length=293, Percent_Identity=26.2798634812287, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472511, Length=293, Percent_Identity=26.2798634812287, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_PSYIN (A1SW94)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943358.1
- ProteinModelPortal:   A1SW94
- SMR:   A1SW94
- STRING:   A1SW94
- GeneID:   4627238
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1994
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   A1SW94
- BioCyc:   PING357804:PING_1994-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 42789; Mature: 42789

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 208-208 ACT_SITE 237-237 ACT_SITE 254-254 BINDING 45-45 BINDING 67-67 BINDING 147-147 BINDING 232-232 BINDING 257-257 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYIDENIPYARDFFTGHGNLHFFSGRNASAEQLIDADVLLVRSITEVNEKLLSLNKKL
CEEEECCCCCCHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
KFVGTATIGTDHIDQTYLKNRGIVFSSAPGCNKVSVAEYILSSLLVLADQQQFILSDKSI
EEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHEECCCCE
AIVGAGNTGTAVYQRLNALGMNCKLYDPPLLLAGDKRQFCTFEEVLEADIISLHVPKVVQ
EEEECCCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHEECCHHHC
GPFPTVHMFDSKVLSQLTNGQILLNAARGDLIDNQALLELAEQGLTPTLVLDVWENEPHI
CCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCC
NQQLLPYVAIATPHIAGYSLDGRVRGTEMLYQALCDFLQLKGKYTTADFVHRAAINSVSI
CHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SKKIDQPLIKSLMHLIFDVRRDDALFREMIEDVDGFDTMRKTYQERRELSTLTVESSVEQ
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEHHHHHH
NTLLQLLGFSTNVQNEIKETHEI
HHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNIYIDENIPYARDFFTGHGNLHFFSGRNASAEQLIDADVLLVRSITEVNEKLLSLNKKL
CEEEECCCCCCHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
KFVGTATIGTDHIDQTYLKNRGIVFSSAPGCNKVSVAEYILSSLLVLADQQQFILSDKSI
EEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHEECCCCE
AIVGAGNTGTAVYQRLNALGMNCKLYDPPLLLAGDKRQFCTFEEVLEADIISLHVPKVVQ
EEEECCCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHEECCHHHC
GPFPTVHMFDSKVLSQLTNGQILLNAARGDLIDNQALLELAEQGLTPTLVLDVWENEPHI
CCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCC
NQQLLPYVAIATPHIAGYSLDGRVRGTEMLYQALCDFLQLKGKYTTADFVHRAAINSVSI
CHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SKKIDQPLIKSLMHLIFDVRRDDALFREMIEDVDGFDTMRKTYQERRELSTLTVESSVEQ
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEHHHHHH
NTLLQLLGFSTNVQNEIKETHEI
HHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA