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The map label for this gene is sppA [H]

Identifier: 119945670

GI number: 119945670

Start: 2412914

End: 2414767

Strand: Direct

Name: sppA [H]

Synonym: Ping_1983

Alternate gene names: 119945670

Gene position: 2412914-2414767 (Clockwise)

Preceding gene: 119945669

Following gene: 119945671

Centisome position: 52.92

GC content: 38.4

Gene sequence:

>1854_bases
ATGAAATTAATCTATTCTTTTTTTAAGAGCATTGCAAAAACCATCAACATCATGCGCTTATTTATTATCAATGTTCTTTT
TTTTGCCATCCTAGCAGCCATTATTTTTGTTATTAACCTCGATCGGCCCGCTCTGTTAGTGGCTGAGAACTCAACCCTGC
ATTTAAATTTCACAGGTAATATAGTAGAGCAGGCAGCACCCGCTGATTTTGCAGGTGAAATATCCAAAAAAATGATTTCA
TCCAATGAAAATCAAAACAACGAATATCAAATTGATAAGATTTTAAATACCATCCATCGCGCACAACAGGATCCCAAAAT
AAGTGCTATTTTATTAGAGCTTGACGGATTAGACGGTGCAGCTATTAATCATATTGCGGATATCGGTAAAGCATTAAATG
ACTTCAAATCAACGGGAAAATCCGTTACTGCCGTGGCCGATAATTACTCTCAGACACAGTATCTGTTAGCAAGTTACGCA
GATAAAATTTACCTTAATCCGCAGGGTATGGTTTTACTGCCGGGTTACTCTGTTTATCGTCTTTATTTTAAAGAGGCATT
AGATAAATTACTTATTACACCACATATATTTAAGGTAGGTACTTACAAATCCTTTGTTGAACCTTTTACAACAAATAAAA
TGTCAGAAGCCAGCAAATTAGCCAATAAAAATTGGTTAGATCAACTGTGGAAAAACTATATTGATACCATTCTTTTCCAG
CGTAAAGATAACAGCAAGATATCGGTACAATCAATATCTCCGACTTTAAAGCAATTAAAATCTTCCCTTATCAAGGCTTC
TGGCAATAGCGCAGTTTATGCATTACAAGTCGGGTTAGTCGATGATCTTAAAAATCGTAATGACGTGATCGACAAGTTAA
AAAATCAGACGCAAACATCCGGTCATAAACTTAATTTAGTCAGTTATGCGGATTATTTACCGACACTGGCAGATATTTAT
GCCGTCAGCGGCGCAAAAGATCAAATTGCTGTTGTTCATGCAAGTGGTGAAATTTTATCTGGAACACAACCTTCCAACGT
GATTGGGGGTGACAGTTTTAGTCAATTATTAGAAACCGCACTCAATGATAATCAAATTAAAGCTGTTGTTATTCGTATTG
ATAGCCCCGGGGGCAGTGCCTTTGCATCGGAAAAAATTCGTCAGCACATTTTAGCCTTAAAAAAAGCAGATAAAAAAGTC
GTTGTCTCAATGGCGAGTGTGGCAGCATCTGGCGGTTACTGGATTGCATCAGCTGCCGATTATATTATCGCCACCCCGAC
TACGTTAACAGGCTCAATAGGCATATTTGGCATGTATGCCAGCGCCGATAAGGCATTAAATAATTTTGGAATATTCAATG
ATGGTATTGGAACGACTGAACTTTCTAGTTTTAGCCCTACACGTGCTCTCAACCCTGAACTTGCTGAAATTATTCAATTA
GGGATAGAAAACGGATATCAACAATTTTTAGAGGTTGTTGCACAGGGTCGCAACATGACGGTAGCAGAGGTAGATAAAAT
AGCACAGGGTCGCGTATGGACCGGTGTAGATGCAAAATCACTTGGTTTGATAGACGAATTAGGAAATTTACAGGATGCTA
TCAATACAGCCAGCAGGTTGGCGAGTCTACAGGATTACGGTGTTAAGATTATTCAACCTGTACTGTCAACCAAACAACAT
CTGATTAATGAATTATTGCTTGGTGCAGTTGAAATTATACCCCAGAGTCTGCATATCAATGCGGCTTTATACCAGACACT
GAATGCAATAGAATCTCAAACGGCAATATTAACGCGGTTTAATGATCCACAGGGACGTTACGCTTACTGCCCTATGTGTG
TTGCAGCTAAATAA

Upstream 100 bases:

>100_bases
TCTAATTTCACTAAAACCTACTAAAATCTACTAAAGCACTGTATATGCCAATATCAGTGCTTTATTTTTATCTTCCCCTC
CTAAAATTTACAGGTTGAAA

Downstream 100 bases:

>100_bases
ATTAGCCCCATCATTAATAGACATAGCATTAATAGATATAACATTAAGAGGCGTCTGAATTTTTCGGGCTTCTCTTCGTT
TTTAATCAATACTGCTGATT

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MKLIYSFFKSIAKTINIMRLFIINVLFFAILAAIIFVINLDRPALLVAENSTLHLNFTGNIVEQAAPADFAGEISKKMIS
SNENQNNEYQIDKILNTIHRAQQDPKISAILLELDGLDGAAINHIADIGKALNDFKSTGKSVTAVADNYSQTQYLLASYA
DKIYLNPQGMVLLPGYSVYRLYFKEALDKLLITPHIFKVGTYKSFVEPFTTNKMSEASKLANKNWLDQLWKNYIDTILFQ
RKDNSKISVQSISPTLKQLKSSLIKASGNSAVYALQVGLVDDLKNRNDVIDKLKNQTQTSGHKLNLVSYADYLPTLADIY
AVSGAKDQIAVVHASGEILSGTQPSNVIGGDSFSQLLETALNDNQIKAVVIRIDSPGGSAFASEKIRQHILALKKADKKV
VVSMASVAASGGYWIASAADYIIATPTTLTGSIGIFGMYASADKALNNFGIFNDGIGTTELSSFSPTRALNPELAEIIQL
GIENGYQQFLEVVAQGRNMTVAEVDKIAQGRVWTGVDAKSLGLIDELGNLQDAINTASRLASLQDYGVKIIQPVLSTKQH
LINELLLGAVEIIPQSLHINAALYQTLNAIESQTAILTRFNDPQGRYAYCPMCVAAK

Sequences:

>Translated_617_residues
MKLIYSFFKSIAKTINIMRLFIINVLFFAILAAIIFVINLDRPALLVAENSTLHLNFTGNIVEQAAPADFAGEISKKMIS
SNENQNNEYQIDKILNTIHRAQQDPKISAILLELDGLDGAAINHIADIGKALNDFKSTGKSVTAVADNYSQTQYLLASYA
DKIYLNPQGMVLLPGYSVYRLYFKEALDKLLITPHIFKVGTYKSFVEPFTTNKMSEASKLANKNWLDQLWKNYIDTILFQ
RKDNSKISVQSISPTLKQLKSSLIKASGNSAVYALQVGLVDDLKNRNDVIDKLKNQTQTSGHKLNLVSYADYLPTLADIY
AVSGAKDQIAVVHASGEILSGTQPSNVIGGDSFSQLLETALNDNQIKAVVIRIDSPGGSAFASEKIRQHILALKKADKKV
VVSMASVAASGGYWIASAADYIIATPTTLTGSIGIFGMYASADKALNNFGIFNDGIGTTELSSFSPTRALNPELAEIIQL
GIENGYQQFLEVVAQGRNMTVAEVDKIAQGRVWTGVDAKSLGLIDELGNLQDAINTASRLASLQDYGVKIIQPVLSTKQH
LINELLLGAVEIIPQSLHINAALYQTLNAIESQTAILTRFNDPQGRYAYCPMCVAAK
>Mature_617_residues
MKLIYSFFKSIAKTINIMRLFIINVLFFAILAAIIFVINLDRPALLVAENSTLHLNFTGNIVEQAAPADFAGEISKKMIS
SNENQNNEYQIDKILNTIHRAQQDPKISAILLELDGLDGAAINHIADIGKALNDFKSTGKSVTAVADNYSQTQYLLASYA
DKIYLNPQGMVLLPGYSVYRLYFKEALDKLLITPHIFKVGTYKSFVEPFTTNKMSEASKLANKNWLDQLWKNYIDTILFQ
RKDNSKISVQSISPTLKQLKSSLIKASGNSAVYALQVGLVDDLKNRNDVIDKLKNQTQTSGHKLNLVSYADYLPTLADIY
AVSGAKDQIAVVHASGEILSGTQPSNVIGGDSFSQLLETALNDNQIKAVVIRIDSPGGSAFASEKIRQHILALKKADKKV
VVSMASVAASGGYWIASAADYIIATPTTLTGSIGIFGMYASADKALNNFGIFNDGIGTTELSSFSPTRALNPELAEIIQL
GIENGYQQFLEVVAQGRNMTVAEVDKIAQGRVWTGVDAKSLGLIDELGNLQDAINTASRLASLQDYGVKIIQPVLSTKQH
LINELLLGAVEIIPQSLHINAALYQTLNAIESQTAILTRFNDPQGRYAYCPMCVAAK

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=622, Percent_Identity=38.4244372990354, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67285; Mature: 67285

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIYSFFKSIAKTINIMRLFIINVLFFAILAAIIFVINLDRPALLVAENSTLHLNFTGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEECCC
IVEQAAPADFAGEISKKMISSNENQNNEYQIDKILNTIHRAQQDPKISAILLELDGLDGA
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
AINHIADIGKALNDFKSTGKSVTAVADNYSQTQYLLASYADKIYLNPQGMVLLPGYSVYR
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCEEEECCHHHHH
LYFKEALDKLLITPHIFKVGTYKSFVEPFTTNKMSEASKLANKNWLDQLWKNYIDTILFQ
HHHHHHHHHHHCCCHHEEECCHHHHHCCHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHEE
RKDNSKISVQSISPTLKQLKSSLIKASGNSAVYALQVGLVDDLKNRNDVIDKLKNQTQTS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHHCCC
GHKLNLVSYADYLPTLADIYAVSGAKDQIAVVHASGEILSGTQPSNVIGGDSFSQLLETA
CCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHH
LNDNQIKAVVIRIDSPGGSAFASEKIRQHILALKKADKKVVVSMASVAASGGYWIASAAD
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCC
YIIATPTTLTGSIGIFGMYASADKALNNFGIFNDGIGTTELSSFSPTRALNPELAEIIQL
EEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GIENGYQQFLEVVAQGRNMTVAEVDKIAQGRVWTGVDAKSLGLIDELGNLQDAINTASRL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ASLQDYGVKIIQPVLSTKQHLINELLLGAVEIIPQSLHINAALYQTLNAIESQTAILTRF
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEC
NDPQGRYAYCPMCVAAK
CCCCCCEEECCEEECCC
>Mature Secondary Structure
MKLIYSFFKSIAKTINIMRLFIINVLFFAILAAIIFVINLDRPALLVAENSTLHLNFTGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEECCC
IVEQAAPADFAGEISKKMISSNENQNNEYQIDKILNTIHRAQQDPKISAILLELDGLDGA
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
AINHIADIGKALNDFKSTGKSVTAVADNYSQTQYLLASYADKIYLNPQGMVLLPGYSVYR
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCEEEECCHHHHH
LYFKEALDKLLITPHIFKVGTYKSFVEPFTTNKMSEASKLANKNWLDQLWKNYIDTILFQ
HHHHHHHHHHHCCCHHEEECCHHHHHCCHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHEE
RKDNSKISVQSISPTLKQLKSSLIKASGNSAVYALQVGLVDDLKNRNDVIDKLKNQTQTS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHHCCC
GHKLNLVSYADYLPTLADIYAVSGAKDQIAVVHASGEILSGTQPSNVIGGDSFSQLLETA
CCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHH
LNDNQIKAVVIRIDSPGGSAFASEKIRQHILALKKADKKVVVSMASVAASGGYWIASAAD
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCC
YIIATPTTLTGSIGIFGMYASADKALNNFGIFNDGIGTTELSSFSPTRALNPELAEIIQL
EEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GIENGYQQFLEVVAQGRNMTVAEVDKIAQGRVWTGVDAKSLGLIDELGNLQDAINTASRL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ASLQDYGVKIIQPVLSTKQHLINELLLGAVEIIPQSLHINAALYQTLNAIESQTAILTRF
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEC
NDPQGRYAYCPMCVAAK
CCCCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]