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The map label for this gene is pncA [H]

Identifier: 119945432

GI number: 119945432

Start: 2104362

End: 2105009

Strand: Direct

Name: pncA [H]

Synonym: Ping_1729

Alternate gene names: 119945432

Gene position: 2104362-2105009 (Clockwise)

Preceding gene: 119945431

Following gene: 119945433

Centisome position: 46.15

GC content: 49.85

Gene sequence:

>648_bases
ATGATTAAAATTAATAAATCCAACACCGCCAGCTTTGATGTCGATCCGCAGCGCGGTTTCACGTCATTGTGTCCCAACGA
GCTACCTATTAGTGAGGGGGAGCAGATTGCTGATCAGTTAAACGCCCAAGCACAGTTTGCAGCTTATCGTCTGGTCAGTA
AGGATTGTCACCCTGCCGAAGCGCCTTGGATTGCCAACTCGACCGATGAGGTAATGACACCCGTTGCTGGAGATTATCCT
AATTTGGATATCAAGTGGCCGGCGCACTGCGTGGTTGGCACTGAAGGGAATCAGCTTATTCCGGGACTGCCGGACGAGGC
AGACTATGATCTGCTGGTTACAAAGGGCACTGATCCGTTTAAACACCCCTATGGCGCTTGCTATCAGGATCTTGGCGAAA
GTGAAAGCACTGGAGCGATTGAATGGTTGCATAAACGGCAGATAAAAGTTGTTATTGTCGGGGGACTGGCAACCGATTAT
TGTGTCAAAATCACTGTACTGCAACTTTGTCTAGCCGGATTTAAAGTGATCGTTAATCTTGGCGCTTGCCGGGGAGTTGC
CAGCGAGACCACTAAAAGTGCCTGTGCGGCAATGTCTGCTGCCGGGGCTGAACTGATTCAAGGCAGTGGGGAAATGGAGT
TGCAATGA

Upstream 100 bases:

>100_bases
ATCAATATTTACAATACGTTAAGCATAGACAGTAAAGCTTACTCTGAGATAGCCAAGTATATGTCCAGCTTTTACCGCTT
TACAGTGGCAGGAGACCCCA

Downstream 100 bases:

>100_bases
GTATAAAAAAGCTGGATTACTGGCTGATTGAGTCGTTGATGGATACAGATCTCTATAAAATTACTATGCTGCAGGCCTAT
TATCATGCGCCGGAGTTCCG

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKINKSNTASFDVDPQRGFTSLCPNELPISEGEQIADQLNAQAQFAAYRLVSKDCHPAEAPWIANSTDEVMTPVAGDYP
NLDIKWPAHCVVGTEGNQLIPGLPDEADYDLLVTKGTDPFKHPYGACYQDLGESESTGAIEWLHKRQIKVVIVGGLATDY
CVKITVLQLCLAGFKVIVNLGACRGVASETTKSACAAMSAAGAELIQGSGEMELQ

Sequences:

>Translated_215_residues
MIKINKSNTASFDVDPQRGFTSLCPNELPISEGEQIADQLNAQAQFAAYRLVSKDCHPAEAPWIANSTDEVMTPVAGDYP
NLDIKWPAHCVVGTEGNQLIPGLPDEADYDLLVTKGTDPFKHPYGACYQDLGESESTGAIEWLHKRQIKVVIVGGLATDY
CVKITVLQLCLAGFKVIVNLGACRGVASETTKSACAAMSAAGAELIQGSGEMELQ
>Mature_215_residues
MIKINKSNTASFDVDPQRGFTSLCPNELPISEGEQIADQLNAQAQFAAYRLVSKDCHPAEAPWIANSTDEVMTPVAGDYP
NLDIKWPAHCVVGTEGNQLIPGLPDEADYDLLVTKGTDPFKHPYGACYQDLGESESTGAIEWLHKRQIKVVIVGGLATDY
CVKITVLQLCLAGFKVIVNLGACRGVASETTKSACAAMSAAGAELIQGSGEMELQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=186, Percent_Identity=38.7096774193548, Blast_Score=90, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23002; Mature: 23002

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKINKSNTASFDVDPQRGFTSLCPNELPISEGEQIADQLNAQAQFAAYRLVSKDCHPAE
CEEECCCCCCEECCCCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCC
APWIANSTDEVMTPVAGDYPNLDIKWPAHCVVGTEGNQLIPGLPDEADYDLLVTKGTDPF
CCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEECCCCEECCCCCCCCCCCEEEECCCCCC
KHPYGACYQDLGESESTGAIEWLHKRQIKVVIVGGLATDYCVKITVLQLCLAGFKVIVNL
CCCHHHHHHHHCCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
GACRGVASETTKSACAAMSAAGAELIQGSGEMELQ
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MIKINKSNTASFDVDPQRGFTSLCPNELPISEGEQIADQLNAQAQFAAYRLVSKDCHPAE
CEEECCCCCCEECCCCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCC
APWIANSTDEVMTPVAGDYPNLDIKWPAHCVVGTEGNQLIPGLPDEADYDLLVTKGTDPF
CCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEECCCCEECCCCCCCCCCCEEEECCCCCC
KHPYGACYQDLGESESTGAIEWLHKRQIKVVIVGGLATDYCVKITVLQLCLAGFKVIVNL
CCCHHHHHHHHCCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
GACRGVASETTKSACAAMSAAGAELIQGSGEMELQ
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]