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The map label for this gene is amtB [C]

Identifier: 119945342

GI number: 119945342

Start: 1984817

End: 1986034

Strand: Direct

Name: amtB [C]

Synonym: Ping_1627

Alternate gene names: 119945342

Gene position: 1984817-1986034 (Clockwise)

Preceding gene: 119945340

Following gene: 119945343

Centisome position: 43.53

GC content: 41.63

Gene sequence:

>1218_bases
ATGGATTTAATACAAAATCATGTTACTGCATTAGTCAGTAGTGCAGATACGTTATTTATTTTATTGGGTGCAATCATGGT
ATTTGCCATGCATGCAGGATTTGCTTTTTTGGAAGTTGGCACTGTGCGCCATAAAAACCAGGTGAACGCGTTAGTTAAAA
TTATGTCTGACTTTGGGATTTCAGCATTAGTTTATTTTTTCTTTGGTTATTGGCTGGCTTACGGTGTTACCTTTTTTGAT
AGTGCCGCGATATTAGCTGAAAATAATGGTTATGAATTGGTTAAATTCTTTTTCTTAATGACCTTTGCTGCCGCAATACC
GGCGATTATCTCAGGTGGTGTTGCTGAGAGAGCTAAATTTAATCCCATGCTAATTGCAGCTGCGTTATGTGTTGGTCTGG
TTTATCCTTTCTTTGAAGGCCTTATTTGGAACGGTAATTATGGATTCCAAGATTGGTTAGCGGCGAACTTTGGTGCGGTA
TTCCACGATTTTGCCGGCTCAATTGTTGTACATGCTGTGGGTGGGTGGATTGCATTTACTGCCATTATTGTTTTGGGCAG
TCGTAATGGCCGTTATCGTGCTGGCAGATTAGTGGCTTTTGCACCCTCTAATATTCCCTTATTAGGACTTGGTGCTTGGA
TTTTATCCGTTGGCTGGTTCGGTTTTAACGTTATGTCTGCTCAAGCTATTGAAGGTATTAGTGGTTTAGTTGCAATGAAC
TCTTTAATGGCGATGGTTGGCGGCATTATTAGTGCACTCGTTATTGGTAAAAAAGATCCGGGTTTTATCCACAATGGTCC
ATTAGCCGGACTCGTCGCAGTCTGTGCTGGTTCCGATATAATGCACCCAATGGGCGCATTGGTGACAGGTTTAGTGGCTG
GTGGTTTGTTTGTATGGTTATTCACTTACGTACAAAAAAAATATACCAGTTTTGACGATGTATTGGGTGTGTGGCCTTTA
CATGGTGTTTGCGGCTTATGGGGCGGTATCTCCGCAGGTATTTTTGGTCTGGAAATCTTTGGTGGTTTAGGTGGTGTCAG
TTTTGCAAGTCAATTAATAGGCTCGTTTGCCGGTGTCTCAGTCGCGCTGATTGGCGGTTTTGCTGTCTACAAACTCGTTA
ATCACTTTATGCCCATTCGCCTCTCTGAAGAAGAAGAATTCAATGGGGCAGATGTTTCAATACATAAAATTGGTGCAACT
AATGTAAACAACCAATAA

Upstream 100 bases:

>100_bases
TTGTTGTTTCTTGTAAAAAACACCTGTAAATATTTTGATATATGCAAAGCATAAGTTAAAATCCGTTAAATTTTTTTTTG
GGTCAACAATAGAGAATTAA

Downstream 100 bases:

>100_bases
GTTGATTGCCGGTTAAAAGGGGATTGCATAAACTATCCCTTTTTTTCCATTAAATAATATCAAAGACAGTTACTGTCATT
AGATGCTTATTCAAGGGGAG

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDLIQNHVTALVSSADTLFILLGAIMVFAMHAGFAFLEVGTVRHKNQVNALVKIMSDFGISALVYFFFGYWLAYGVTFFD
SAAILAENNGYELVKFFFLMTFAAAIPAIISGGVAERAKFNPMLIAAALCVGLVYPFFEGLIWNGNYGFQDWLAANFGAV
FHDFAGSIVVHAVGGWIAFTAIIVLGSRNGRYRAGRLVAFAPSNIPLLGLGAWILSVGWFGFNVMSAQAIEGISGLVAMN
SLMAMVGGIISALVIGKKDPGFIHNGPLAGLVAVCAGSDIMHPMGALVTGLVAGGLFVWLFTYVQKKYTSFDDVLGVWPL
HGVCGLWGGISAGIFGLEIFGGLGGVSFASQLIGSFAGVSVALIGGFAVYKLVNHFMPIRLSEEEEFNGADVSIHKIGAT
NVNNQ

Sequences:

>Translated_405_residues
MDLIQNHVTALVSSADTLFILLGAIMVFAMHAGFAFLEVGTVRHKNQVNALVKIMSDFGISALVYFFFGYWLAYGVTFFD
SAAILAENNGYELVKFFFLMTFAAAIPAIISGGVAERAKFNPMLIAAALCVGLVYPFFEGLIWNGNYGFQDWLAANFGAV
FHDFAGSIVVHAVGGWIAFTAIIVLGSRNGRYRAGRLVAFAPSNIPLLGLGAWILSVGWFGFNVMSAQAIEGISGLVAMN
SLMAMVGGIISALVIGKKDPGFIHNGPLAGLVAVCAGSDIMHPMGALVTGLVAGGLFVWLFTYVQKKYTSFDDVLGVWPL
HGVCGLWGGISAGIFGLEIFGGLGGVSFASQLIGSFAGVSVALIGGFAVYKLVNHFMPIRLSEEEEFNGADVSIHKIGAT
NVNNQ
>Mature_405_residues
MDLIQNHVTALVSSADTLFILLGAIMVFAMHAGFAFLEVGTVRHKNQVNALVKIMSDFGISALVYFFFGYWLAYGVTFFD
SAAILAENNGYELVKFFFLMTFAAAIPAIISGGVAERAKFNPMLIAAALCVGLVYPFFEGLIWNGNYGFQDWLAANFGAV
FHDFAGSIVVHAVGGWIAFTAIIVLGSRNGRYRAGRLVAFAPSNIPLLGLGAWILSVGWFGFNVMSAQAIEGISGLVAMN
SLMAMVGGIISALVIGKKDPGFIHNGPLAGLVAVCAGSDIMHPMGALVTGLVAGGLFVWLFTYVQKKYTSFDDVLGVWPL
HGVCGLWGGISAGIFGLEIFGGLGGVSFASQLIGSFAGVSVALIGGFAVYKLVNHFMPIRLSEEEEFNGADVSIHKIGAT
NVNNQ

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=416, Percent_Identity=30.2884615384615, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17550182, Length=427, Percent_Identity=29.7423887587822, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=28.4064665127021, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=26.8065268065268, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=449, Percent_Identity=27.1714922048998, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325396, Length=360, Percent_Identity=27.5, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321559, Length=410, Percent_Identity=27.3170731707317, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=410, Percent_Identity=27.0731707317073, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078314, Length=429, Percent_Identity=29.6037296037296, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42912; Mature: 42912

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIQNHVTALVSSADTLFILLGAIMVFAMHAGFAFLEVGTVRHKNQVNALVKIMSDFGI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHCCH
SALVYFFFGYWLAYGVTFFDSAAILAENNGYELVKFFFLMTFAAAIPAIISGGVAERAKF
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
NPMLIAAALCVGLVYPFFEGLIWNGNYGFQDWLAANFGAVFHDFAGSIVVHAVGGWIAFT
CHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIIVLGSRNGRYRAGRLVAFAPSNIPLLGLGAWILSVGWFGFNVMSAQAIEGISGLVAMN
HHHHCCCCCCCEECCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMAMVGGIISALVIGKKDPGFIHNGPLAGLVAVCAGSDIMHPMGALVTGLVAGGLFVWL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTYVQKKYTSFDDVLGVWPLHGVCGLWGGISAGIFGLEIFGGLGGVSFASQLIGSFAGVS
HHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VALIGGFAVYKLVNHFMPIRLSEEEEFNGADVSIHKIGATNVNNQ
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MDLIQNHVTALVSSADTLFILLGAIMVFAMHAGFAFLEVGTVRHKNQVNALVKIMSDFGI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHCCH
SALVYFFFGYWLAYGVTFFDSAAILAENNGYELVKFFFLMTFAAAIPAIISGGVAERAKF
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
NPMLIAAALCVGLVYPFFEGLIWNGNYGFQDWLAANFGAVFHDFAGSIVVHAVGGWIAFT
CHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIIVLGSRNGRYRAGRLVAFAPSNIPLLGLGAWILSVGWFGFNVMSAQAIEGISGLVAMN
HHHHCCCCCCCEECCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMAMVGGIISALVIGKKDPGFIHNGPLAGLVAVCAGSDIMHPMGALVTGLVAGGLFVWL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTYVQKKYTSFDDVLGVWPLHGVCGLWGGISAGIFGLEIFGGLGGVSFASQLIGSFAGVS
HHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VALIGGFAVYKLVNHFMPIRLSEEEEFNGADVSIHKIGATNVNNQ
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]