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The map label for this gene is pfkA [H]

Identifier: 119945060

GI number: 119945060

Start: 1636478

End: 1637440

Strand: Direct

Name: pfkA [H]

Synonym: Ping_1316

Alternate gene names: 119945060

Gene position: 1636478-1637440 (Clockwise)

Preceding gene: 119945059

Following gene: 119945061

Centisome position: 35.89

GC content: 42.06

Gene sequence:

>963_bases
ATGATTAAAAAAATTGGTGTTTTAACAAGCGGCGGTGATGCACCGGGTATGAATTCAGCAATACGTGCAGTCGTGCGCTG
TGCTCTGGCAAATGATATTGAAGTCTATGGTATTTATGATGGTTATGCCGGACTTCATAAAGGTAATCTCAAACAGTTAA
CGCGTCATTCTGTTTCGGATATGATTAACCGTGGTGGTACTTTCTTGGGCAGTGCCCGTTTTCCTGAATTTAAAGAAGAA
AGTGTCCGTCAGGAAGCGATTAAAAATCTAGAAAAGCACGGTATTGAAGCATTAGTTGTGATCGGTGGTGATGGTTCTTA
TATGGGGGCGAAAAAACTCAGTGAAATGGGTTATCCCTGTATCGGTATTCCTGGAACAATTGATAATGATATTGCCGGAA
CAGATTATACTATCGGATTTATGACCTGCTTAAATACCATTGTTGATGCGGTAGACCGTTTACGTGATACCTCTTCATCA
CATAACCGTATCTCTATTGTTGAAGTAATGGGTCGCTATTGTGGTGATTTAACGCTTTGGTCTGCCATTGCTGGCGGCAG
TGAATATGTTGTCCTGCCGGAAATAGGTTTTGACGAAGCTGAATTATTTGAGCATATTGAAACGGGTGAAGCCTCGGGTA
AAAAGCATGCCATTATTATGATCACTGAACATGTAACGGATGTGACAGAATTGGCTAAACGTATTGAAGCAAAGACCGGC
CGAGAAACGCGTGCCACTATTTTAGGCCATATCCAACGTGGTGGTTCACCTATGGCGTTTGATCGCGTATTAGCAAGTCG
TATGGGTGCCCGTGCAGTTGAGCTTTTGATTGATGGTAAAAGTGCGCGTTGTATCGGTCTTCAGTATGGCAAGATGGTTG
ACCATGATATTATTGAATGTTTAGAAGAGATGGAGCGTCCGTTCCGTGAAGATTTATACAATTTATCGAACGTGTTATTC
TAA

Upstream 100 bases:

>100_bases
AATTTTAAGGTAGGCAGATATAAGTTCAGGATATCCCTTCCGAATCACATATTTCGAATATTGACTTAGCAGTTACAGCA
GTAACAGGATGAGGTAGCAA

Downstream 100 bases:

>100_bases
TAATAAGCCGATTAATTTAAATGATCTAAGCCTACCTTATGGTAGGCTTTTTTTTGGCTCGGTGCTAACGCTGTAATCCT
TAAAATTACAATACGTTAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGVLTSGGDAPGMNSAIRAVVRCALANDIEVYGIYDGYAGLHKGNLKQLTRHSVSDMINRGGTFLGSARFPEFKEE
SVRQEAIKNLEKHGIEALVVIGGDGSYMGAKKLSEMGYPCIGIPGTIDNDIAGTDYTIGFMTCLNTIVDAVDRLRDTSSS
HNRISIVEVMGRYCGDLTLWSAIAGGSEYVVLPEIGFDEAELFEHIETGEASGKKHAIIMITEHVTDVTELAKRIEAKTG
RETRATILGHIQRGGSPMAFDRVLASRMGARAVELLIDGKSARCIGLQYGKMVDHDIIECLEEMERPFREDLYNLSNVLF

Sequences:

>Translated_320_residues
MIKKIGVLTSGGDAPGMNSAIRAVVRCALANDIEVYGIYDGYAGLHKGNLKQLTRHSVSDMINRGGTFLGSARFPEFKEE
SVRQEAIKNLEKHGIEALVVIGGDGSYMGAKKLSEMGYPCIGIPGTIDNDIAGTDYTIGFMTCLNTIVDAVDRLRDTSSS
HNRISIVEVMGRYCGDLTLWSAIAGGSEYVVLPEIGFDEAELFEHIETGEASGKKHAIIMITEHVTDVTELAKRIEAKTG
RETRATILGHIQRGGSPMAFDRVLASRMGARAVELLIDGKSARCIGLQYGKMVDHDIIECLEEMERPFREDLYNLSNVLF
>Mature_320_residues
MIKKIGVLTSGGDAPGMNSAIRAVVRCALANDIEVYGIYDGYAGLHKGNLKQLTRHSVSDMINRGGTFLGSARFPEFKEE
SVRQEAIKNLEKHGIEALVVIGGDGSYMGAKKLSEMGYPCIGIPGTIDNDIAGTDYTIGFMTCLNTIVDAVDRLRDTSSS
HNRISIVEVMGRYCGDLTLWSAIAGGSEYVVLPEIGFDEAELFEHIETGEASGKKHAIIMITEHVTDVTELAKRIEAKTG
RETRATILGHIQRGGSPMAFDRVLASRMGARAVELLIDGKSARCIGLQYGKMVDHDIIECLEEMERPFREDLYNLSNVLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=339, Percent_Identity=39.5280235988201, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI266453619, Length=338, Percent_Identity=38.4615384615385, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI266453768, Length=338, Percent_Identity=38.4615384615385, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI266453748, Length=338, Percent_Identity=38.4615384615385, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI4505749, Length=338, Percent_Identity=38.4615384615385, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI48762920, Length=338, Percent_Identity=39.0532544378698, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=67.8125, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25147584, Length=341, Percent_Identity=36.3636363636364, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=37.8205128205128, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=32.5259515570934, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321679, Length=339, Percent_Identity=36.5781710914454, Blast_Score=198, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=33.7243401759531, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28573326, Length=342, Percent_Identity=36.2573099415205, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24652337, Length=341, Percent_Identity=36.3636363636364, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17647809, Length=341, Percent_Identity=36.3636363636364, Blast_Score=198, Evalue=4e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34922; Mature: 34922

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGVLTSGGDAPGMNSAIRAVVRCALANDIEVYGIYDGYAGLHKGNLKQLTRHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
MINRGGTFLGSARFPEFKEESVRQEAIKNLEKHGIEALVVIGGDGSYMGAKKLSEMGYPC
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCE
IGIPGTIDNDIAGTDYTIGFMTCLNTIVDAVDRLRDTSSSHNRISIVEVMGRYCGDLTLW
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHH
SAIAGGSEYVVLPEIGFDEAELFEHIETGEASGKKHAIIMITEHVTDVTELAKRIEAKTG
HHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
RETRATILGHIQRGGSPMAFDRVLASRMGARAVELLIDGKSARCIGLQYGKMVDHDIIEC
CHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEEEECCCCEEEEEHHCCHHHHHHHHH
LEEMERPFREDLYNLSNVLF
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIGVLTSGGDAPGMNSAIRAVVRCALANDIEVYGIYDGYAGLHKGNLKQLTRHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
MINRGGTFLGSARFPEFKEESVRQEAIKNLEKHGIEALVVIGGDGSYMGAKKLSEMGYPC
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCE
IGIPGTIDNDIAGTDYTIGFMTCLNTIVDAVDRLRDTSSSHNRISIVEVMGRYCGDLTLW
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHH
SAIAGGSEYVVLPEIGFDEAELFEHIETGEASGKKHAIIMITEHVTDVTELAKRIEAKTG
HHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
RETRATILGHIQRGGSPMAFDRVLASRMGARAVELLIDGKSARCIGLQYGKMVDHDIIEC
CHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEEEECCCCEEEEEHHCCHHHHHHHHH
LEEMERPFREDLYNLSNVLF
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA