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The map label for this gene is sbcD [H]

Identifier: 119945048

GI number: 119945048

Start: 1613212

End: 1614438

Strand: Direct

Name: sbcD [H]

Synonym: Ping_1302

Alternate gene names: 119945048

Gene position: 1613212-1614438 (Clockwise)

Preceding gene: 119945047

Following gene: 119945049

Centisome position: 35.38

GC content: 41.24

Gene sequence:

>1227_bases
ATGGACAAATTAATGAAGATATTACACACTTCAGACTGGCATCTTGGTCAGTATTTTATGATGAAAACCAGAGAGTCAGA
GCATTTACATTTTTTAAACTGGTTAATTGAAGTGGTCAATAAGCAACAGATCGATGCATTAATCGTTGCCGGTGATATTT
TTGATAGCGCATCCCCGCCTTCCTACGCGCGCAAATTATACAGTGATTTTATTGTCAGATTGCAAAAAAGCAGCTGTCGC
CAGCTTATTATTATGGCGGGGAATCATGACAGTATTGCCGTGCTCAATGAAAACAAAGATTTATTAAAAGCATTGGATGT
TTCTGTATTAGCCGGATTAAGTGATGATTTAAACGAGCACATTATTGAACTTAAGGATGATAGGCAAAATACGCAGGCAT
TAGTTTGTGCATTGCCTTTTTTACGGGCAGCAGATGTGATGAGAAGTGTTCAGGGTAGTTCGGCCGCTGATAAACAGATT
TCTCTGCAGCAGGGCATAGCGGACACCTACCAGTCGATTTTCGAACTCGCTAATGCGCAGTGTCATGAGAAAGCCCTTCC
TATTATTGCAACCGGGCATCTGACTGCGGTGGGCTGCAGTGTTTCTGACTCGGTGCGTGAAATTTATATCGGCACACTGA
CCGCTTTCCCTGCTGTGCTCTTCCCTGCTTTTAACTATATTGCCCTGGGACATTTACACCGTGCGCAAAAAGTACAAAAA
ACCGACCATATTCGTTATTGCGGCAGCCCGATTGCCTTGAGCTTTGATGAAACCAGGCAGCAGAAAAATATTAATATTAT
TGAATTTGATACAAATAATGCCTTAACCGTTAATGAGACGGTGATCCCTGTTTTTCAGAGAATGCAGGTTATCAGCGGAG
ATTTAGAATCTGTAATAGAGCAGTTACAAGAGCTTAAAAAAGAGTTAGCGGGTGAAAGTGCCTGGATAGAGGTCAAGCTC
AAGCAGGCTTTTTATCTTTCCGATGTGCATGCATTATTAAATGAACAGGTCCAGGAAAGTCAAATAGAAATTTTAAAAAT
CAGCAGTCCGACTATTAATGAAAAAAATCAGTATCAGGCTGCGGATAAATCAACGCTGGATAATTTAACACCGGCAGATC
TTTTTCAGCATCGTTTACAGATTGAAGACAGCTACAGTGACCAGCAGAGAGAGCAGTTAACTAAACTGTTTGCCGAAGTC
TGCTCTGAAGTGGAGCATAAATCATGA

Upstream 100 bases:

>100_bases
GCGCGAATATCGGTAATTTTTAATATCAACATCCTTGTTTATTATTCAAAACAGTTTTTTAGGTCTTAAATGGGTAAACT
TAATCCTTTACCGCTAAGCA

Downstream 100 bases:

>100_bases
AAATATTATCTATTCGTTTTAAAAATCTAAATTCATTAAAAGGCCATTGGAAAATTGATTTTCAGGATGCTGATTTTTTA
GAAAACGGTCTGTTTGTTAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MDKLMKILHTSDWHLGQYFMMKTRESEHLHFLNWLIEVVNKQQIDALIVAGDIFDSASPPSYARKLYSDFIVRLQKSSCR
QLIIMAGNHDSIAVLNENKDLLKALDVSVLAGLSDDLNEHIIELKDDRQNTQALVCALPFLRAADVMRSVQGSSAADKQI
SLQQGIADTYQSIFELANAQCHEKALPIIATGHLTAVGCSVSDSVREIYIGTLTAFPAVLFPAFNYIALGHLHRAQKVQK
TDHIRYCGSPIALSFDETRQQKNINIIEFDTNNALTVNETVIPVFQRMQVISGDLESVIEQLQELKKELAGESAWIEVKL
KQAFYLSDVHALLNEQVQESQIEILKISSPTINEKNQYQAADKSTLDNLTPADLFQHRLQIEDSYSDQQREQLTKLFAEV
CSEVEHKS

Sequences:

>Translated_408_residues
MDKLMKILHTSDWHLGQYFMMKTRESEHLHFLNWLIEVVNKQQIDALIVAGDIFDSASPPSYARKLYSDFIVRLQKSSCR
QLIIMAGNHDSIAVLNENKDLLKALDVSVLAGLSDDLNEHIIELKDDRQNTQALVCALPFLRAADVMRSVQGSSAADKQI
SLQQGIADTYQSIFELANAQCHEKALPIIATGHLTAVGCSVSDSVREIYIGTLTAFPAVLFPAFNYIALGHLHRAQKVQK
TDHIRYCGSPIALSFDETRQQKNINIIEFDTNNALTVNETVIPVFQRMQVISGDLESVIEQLQELKKELAGESAWIEVKL
KQAFYLSDVHALLNEQVQESQIEILKISSPTINEKNQYQAADKSTLDNLTPADLFQHRLQIEDSYSDQQREQLTKLFAEV
CSEVEHKS
>Mature_408_residues
MDKLMKILHTSDWHLGQYFMMKTRESEHLHFLNWLIEVVNKQQIDALIVAGDIFDSASPPSYARKLYSDFIVRLQKSSCR
QLIIMAGNHDSIAVLNENKDLLKALDVSVLAGLSDDLNEHIIELKDDRQNTQALVCALPFLRAADVMRSVQGSSAADKQI
SLQQGIADTYQSIFELANAQCHEKALPIIATGHLTAVGCSVSDSVREIYIGTLTAFPAVLFPAFNYIALGHLHRAQKVQK
TDHIRYCGSPIALSFDETRQQKNINIIEFDTNNALTVNETVIPVFQRMQVISGDLESVIEQLQELKKELAGESAWIEVKL
KQAFYLSDVHALLNEQVQESQIEILKISSPTINEKNQYQAADKSTLDNLTPADLFQHRLQIEDSYSDQQREQLTKLFAEV
CSEVEHKS

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=394, Percent_Identity=41.8781725888325, Blast_Score=328, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: 3.1.-.-

Molecular weight: Translated: 45967; Mature: 45967

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLMKILHTSDWHLGQYFMMKTRESEHLHFLNWLIEVVNKQQIDALIVAGDIFDSASPP
CHHHHHHHHCCCCCCCHHHEEECCCCCHHHHHHHHHHHHCHHHHCEEEEECHHCCCCCCH
SYARKLYSDFIVRLQKSSCRQLIIMAGNHDSIAVLNENKDLLKALDVSVLAGLSDDLNEH
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHH
IIELKDDRQNTQALVCALPFLRAADVMRSVQGSSAADKQISLQQGIADTYQSIFELANAQ
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
CHEKALPIIATGHLTAVGCSVSDSVREIYIGTLTAFPAVLFPAFNYIALGHLHRAQKVQK
HHHCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDHIRYCGSPIALSFDETRQQKNINIIEFDTNNALTVNETVIPVFQRMQVISGDLESVIE
HCCHHCCCCCEEECCHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHHCHHHHHHH
QLQELKKELAGESAWIEVKLKQAFYLSDVHALLNEQVQESQIEILKISSPTINEKNQYQA
HHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
ADKSTLDNLTPADLFQHRLQIEDSYSDQQREQLTKLFAEVCSEVEHKS
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKLMKILHTSDWHLGQYFMMKTRESEHLHFLNWLIEVVNKQQIDALIVAGDIFDSASPP
CHHHHHHHHCCCCCCCHHHEEECCCCCHHHHHHHHHHHHCHHHHCEEEEECHHCCCCCCH
SYARKLYSDFIVRLQKSSCRQLIIMAGNHDSIAVLNENKDLLKALDVSVLAGLSDDLNEH
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHH
IIELKDDRQNTQALVCALPFLRAADVMRSVQGSSAADKQISLQQGIADTYQSIFELANAQ
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
CHEKALPIIATGHLTAVGCSVSDSVREIYIGTLTAFPAVLFPAFNYIALGHLHRAQKVQK
HHHCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDHIRYCGSPIALSFDETRQQKNINIIEFDTNNALTVNETVIPVFQRMQVISGDLESVIE
HCCHHCCCCCEEECCHHHHHHCCCEEEEECCCCEEEECHHHHHHHHHHHHHHCHHHHHHH
QLQELKKELAGESAWIEVKLKQAFYLSDVHALLNEQVQESQIEILKISSPTINEKNQYQA
HHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
ADKSTLDNLTPADLFQHRLQIEDSYSDQQREQLTKLFAEVCSEVEHKS
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]