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The map label for this gene is rlmH

Identifier: 119944892

GI number: 119944892

Start: 1424817

End: 1425287

Strand: Reverse

Name: rlmH

Synonym: Ping_1136

Alternate gene names: 119944892

Gene position: 1425287-1424817 (Counterclockwise)

Preceding gene: 119944893

Following gene: 119944891

Centisome position: 31.26

GC content: 42.89

Gene sequence:

>471_bases
ATGAAAATTCAGTTAATCGCAGTCGGCACAAAGATGCCCGACTGGGTTGAAAAAGGATACCAAGAATATGCGCGACGCTT
TCCAAAAGAGATGCAACTTGAATTGCTGGAAATCAATGCTGGGAAGCGTGGTAAAAATGCGGATATAAAACGGATATTGA
AAAAAGAGGGGGAATTGACCCTTGCAGCAATCCCCAAAGGTAATAAAATTGTCACACTGGAAGTGACGGGACAAGCATGG
ACAACTGAACAGCTTGCCGATGAGATGGGCAAATGGCAGTTAGATGCAAGAAATATTAGTTTATTAATCGGTGGACCTGA
AGGGCTTGCGCCTGAATGCATTACAAAGTCTGAACAACGCTGGTCACTTTCAGCGTTAACCTTGCCCCATCCTTTAGTAC
GAGTTATCGTCGCCGAAAGTTTATATCGCGCTTTTACTTTAACAATTAACCACCCATACCATAGAGAATAA

Upstream 100 bases:

>100_bases
AGTGGGTATTACTGGACTTGGGTAATGTTGTTGTCCACGTTATGCAGGATGAAACAAGACAGTTTTATCAACTTGAAAAA
CTTTGGGAACAGTCTGCTTA

Downstream 100 bases:

>100_bases
TACGACGTGGTTAAAAAACGCATTCCCATAAGGAATCATTCCGCAGAATCCGCACTCTTTATGCGCCGTACTCTGGTAAT
TTTTATTGCGATAGTCATTG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKIQLIAVGTKMPDWVEKGYQEYARRFPKEMQLELLEINAGKRGKNADIKRILKKEGELTLAAIPKGNKIVTLEVTGQAW
TTEQLADEMGKWQLDARNISLLIGGPEGLAPECITKSEQRWSLSALTLPHPLVRVIVAESLYRAFTLTINHPYHRE

Sequences:

>Translated_156_residues
MKIQLIAVGTKMPDWVEKGYQEYARRFPKEMQLELLEINAGKRGKNADIKRILKKEGELTLAAIPKGNKIVTLEVTGQAW
TTEQLADEMGKWQLDARNISLLIGGPEGLAPECITKSEQRWSLSALTLPHPLVRVIVAESLYRAFTLTINHPYHRE
>Mature_156_residues
MKIQLIAVGTKMPDWVEKGYQEYARRFPKEMQLELLEINAGKRGKNADIKRILKKEGELTLAAIPKGNKIVTLEVTGQAW
TTEQLADEMGKWQLDARNISLLIGGPEGLAPECITKSEQRWSLSALTLPHPLVRVIVAESLYRAFTLTINHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=70.5128205128205, Blast_Score=232, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PSYIN (A1SU08)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942572.1
- ProteinModelPortal:   A1SU08
- SMR:   A1SU08
- STRING:   A1SU08
- GeneID:   4623877
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1136
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   A1SU08
- ProtClustDB:   PRK00103
- BioCyc:   PING357804:PING_1136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17663; Mature: 17663

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLIAVGTKMPDWVEKGYQEYARRFPKEMQLELLEINAGKRGKNADIKRILKKEGELT
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCCHHHHHHHHHCCCEE
LAAIPKGNKIVTLEVTGQAWTTEQLADEMGKWQLDARNISLLIGGPEGLAPECITKSEQR
EEEECCCCEEEEEEECCCCCCHHHHHHHHCCEEECCCCEEEEEECCCCCCHHHHCCCCCC
WSLSALTLPHPLVRVIVAESLYRAFTLTINHPYHRE
EEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCC
>Mature Secondary Structure
MKIQLIAVGTKMPDWVEKGYQEYARRFPKEMQLELLEINAGKRGKNADIKRILKKEGELT
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCCHHHHHHHHHCCCEE
LAAIPKGNKIVTLEVTGQAWTTEQLADEMGKWQLDARNISLLIGGPEGLAPECITKSEQR
EEEECCCCEEEEEEECCCCCCHHHHHHHHCCEEECCCCEEEEEECCCCCCHHHHCCCCCC
WSLSALTLPHPLVRVIVAESLYRAFTLTINHPYHRE
EEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA