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The map label for this gene is nasB [H]

Identifier: 119944782

GI number: 119944782

Start: 1289153

End: 1291678

Strand: Reverse

Name: nasB [H]

Synonym: Ping_1024

Alternate gene names: 119944782

Gene position: 1291678-1289153 (Counterclockwise)

Preceding gene: 119944783

Following gene: 119944781

Centisome position: 28.33

GC content: 44.54

Gene sequence:

>2526_bases
ATGAACAAACAACGCATTGTGGTGGTCGGTAACGGCATGGTCGGCCACAAGTTTATCGATACTCTGATTACAGACGAAAG
TGTTGACTACGATATCATTACCTTTTCAGAAGAATCTCGCCTTGCCTATGATCGCGTGCAACTCAGTGGCTTTTTCTCGG
GTAAAACAGCCGATGATTTAGCCCTGACCGATGCAACTTACTATCAAGAAAACGGTATTAACTTTGTGCTCAACGATAAA
GTTGTCAAACTTGATACCAAAAATAATCAGGTGGTCACCAGCAGTGGCCGGATTGAATCCTACGATAAATTAGTGCTGGC
AACGGGTTCATTTCCTTTTGTGCCCCCCATTTCAGGCAATGATAAAGAACATTGTCATGTCTACCGTACTATTGATGATT
TAGAGGACATAACGGCCTCTGGAAAAAGCAGTAAAGTAGGGGTTGTTGTTGGTGGCGGTCTGTTAGGTCTTGAAGCTGCC
AATGCACTGGTTAATCTTGGTTTGGAAACCCATGTGGTGGAATTTGCACCACGATTAATGGCTGTGCAGTTAGATGACGG
GGGTGGTCAATTACTGCGCCGTAAAATTGAAGCATTAGGCGTACAAGTACACACCGAAAAAGCGACCACGGAAATTGTCG
ATGGTGATAACTGCCGCTACCGGATGAACTTTGCCGATGGCAGTTTCTTAGAAACCGATACGATTGTTTTCTCTGCGGGT
ATTCGCCCTCAGGATGAACTGGCCAGGCAGTTTAATTTAACGATTGGCGAGCGTGGCGGCATTGCTATCAATAACCAGTG
CCAGACTAATGTTGCAAATATTTATGCGATTGGTGAATGCGCGTTATGGGATAACAGAATTTTTGGTTTAGTGGCCCCCG
GTTACCAAATGGCAAAAGTTGCCGCAGCCCATATTAAAGGTGAATCACTTGCCTTTACGGGGGCTGATATGAGCACCAAA
CTTAAATTATTAGGCATTGATGTTGCCAGTATTGGCGAAGTACACGGTCAGACACCCGGCGCACAGTCTTATACCTACCA
CGATGAAATTGAACAGGTTTATAAACGTTTAATTGTCTCGGAAGACGGCAAAAAAATTGTCGGCGCAGTGTTAGTCGGAG
ATGCAGAGGCTTACGGCACATTATTACAACTGTTACTCAATGATATGACCCTGCCGGAAAACCCAGCGGTATTGATTCTA
CCCAGTACCGGTGAAGAACAAGCCGCTGGATTCGGAGTCGAAGCCCTGCCCGAAACAGCGACTATCTGCTCATGTTTTGA
TGTCTCTAAAGGCGATCTTAAAAGCGCGGTGATCGCAGGTTGCTGCACTATGGGCGATCTCAAAACGCAAACCAAAGCAT
CAACAGGCTGTGGTGGCTGCAGTGCACTGACTAAACAAGTATTAGACAGTGAATTAGCGAGCTTAGGTTTTGAAGTCAAT
AACGATCTTTGCGAACATTTTGCCTACTCAAGACAAGAACTTACGGACATTATTCGGGTTAAAAAAATCACCACCTTTGA
TCAATTACTGGAAAATTACGGCAGCGGTTTAGGCTGTGAAATTTGTAAACCCACCGCCGGCTCTATTTTAGCCTCCTACT
GGAATGAATATGTATTAGAAGATACCCATCTTGAATTACAGGACAGTAACGATCTCTATCTTGGTAATATTCAAAAAAAC
GGTACTTATTCCGTCGTACCACGGATAGCAGGTGGTGAAATCACCCCGGATAAATTGATTGTGCTCGGTGAAGTTGCCAA
GAAATTTGATCTTTATACCAAAATAACCGGTGGTCAGCGGATAGATTTATTTGGCGCGCAGTTACATGAATTACCGGTTA
TTTGGCAGGAACTGATCGATGCCGGTTTTGAAACCGGTCACGCTTACGGTAAATCCTTACGCACAGTGAAATCCTGTGTC
GGTAATACCTGGTGCCGTTTTGGGGTTGATGACAGCATGACCTTAGCCCTCAATCTTGAAAATCGTTATAAAGGTTTGCG
TTCCCCGCATAAAATAAAATTTGCCGTATCCGGTTGTACCCGCGAATGTGCCGAAGCCCAGAGCAAAGATATTGGCCTTA
TTTCCACCGAAAATGGCTGGAATATGTATGTCTGCGGTAATGGGGGTATGCGCCCCCGTCACGGCGATTTATTTGCCTCA
GGCCTTGATGAGCAAACCCTTATAAAATATATAGACCGCTTTTTAATGTTCTATGTCCGCACCGCAGATCGCCTGCAGCG
TACGTCAGTTTGGATGGAAAATATGGAAGGCGGATTAGATTACCTAAGGGAAGTGGTGATTGAGGATAAGCTTAATCTGG
CCGATGAACTTGAGCAGGAGATGGAAAATAACATTAAAAACTATCAATGTGAATGGAAAACCACGCTGCAAAATCCAGAA
AAACTAAAACGCTTTAAACACTTTATCAACAGTGATGACACCGATAATCAGTTGGCATTTGAACTCGAGCGCGAGCAGCG
TATACCCGCCCAGCAAACAATCAAAGTCCTAGAATTAACGGCATAA

Upstream 100 bases:

>100_bases
CTGAGCAATTACAGTGGTTCAACGAAAAACCATCAATCGATAGTGCGATTAATGAGTTAACACAATTATCAGCTTAAAAA
TTTAAAAGGATTTTGACATT

Downstream 100 bases:

>100_bases
GGAAAAACTCATGAGCCAGTGGTTTAATATTTGTAAGTATGAAGATCTTGCTCCTAACAGCGGCATTTGTGCCCAGTTTG
AAAGTCAGCAAGTCGCGATT

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 841; Mature: 841

Protein sequence:

>841_residues
MNKQRIVVVGNGMVGHKFIDTLITDESVDYDIITFSEESRLAYDRVQLSGFFSGKTADDLALTDATYYQENGINFVLNDK
VVKLDTKNNQVVTSSGRIESYDKLVLATGSFPFVPPISGNDKEHCHVYRTIDDLEDITASGKSSKVGVVVGGGLLGLEAA
NALVNLGLETHVVEFAPRLMAVQLDDGGGQLLRRKIEALGVQVHTEKATTEIVDGDNCRYRMNFADGSFLETDTIVFSAG
IRPQDELARQFNLTIGERGGIAINNQCQTNVANIYAIGECALWDNRIFGLVAPGYQMAKVAAAHIKGESLAFTGADMSTK
LKLLGIDVASIGEVHGQTPGAQSYTYHDEIEQVYKRLIVSEDGKKIVGAVLVGDAEAYGTLLQLLLNDMTLPENPAVLIL
PSTGEEQAAGFGVEALPETATICSCFDVSKGDLKSAVIAGCCTMGDLKTQTKASTGCGGCSALTKQVLDSELASLGFEVN
NDLCEHFAYSRQELTDIIRVKKITTFDQLLENYGSGLGCEICKPTAGSILASYWNEYVLEDTHLELQDSNDLYLGNIQKN
GTYSVVPRIAGGEITPDKLIVLGEVAKKFDLYTKITGGQRIDLFGAQLHELPVIWQELIDAGFETGHAYGKSLRTVKSCV
GNTWCRFGVDDSMTLALNLENRYKGLRSPHKIKFAVSGCTRECAEAQSKDIGLISTENGWNMYVCGNGGMRPRHGDLFAS
GLDEQTLIKYIDRFLMFYVRTADRLQRTSVWMENMEGGLDYLREVVIEDKLNLADELEQEMENNIKNYQCEWKTTLQNPE
KLKRFKHFINSDDTDNQLAFELEREQRIPAQQTIKVLELTA

Sequences:

>Translated_841_residues
MNKQRIVVVGNGMVGHKFIDTLITDESVDYDIITFSEESRLAYDRVQLSGFFSGKTADDLALTDATYYQENGINFVLNDK
VVKLDTKNNQVVTSSGRIESYDKLVLATGSFPFVPPISGNDKEHCHVYRTIDDLEDITASGKSSKVGVVVGGGLLGLEAA
NALVNLGLETHVVEFAPRLMAVQLDDGGGQLLRRKIEALGVQVHTEKATTEIVDGDNCRYRMNFADGSFLETDTIVFSAG
IRPQDELARQFNLTIGERGGIAINNQCQTNVANIYAIGECALWDNRIFGLVAPGYQMAKVAAAHIKGESLAFTGADMSTK
LKLLGIDVASIGEVHGQTPGAQSYTYHDEIEQVYKRLIVSEDGKKIVGAVLVGDAEAYGTLLQLLLNDMTLPENPAVLIL
PSTGEEQAAGFGVEALPETATICSCFDVSKGDLKSAVIAGCCTMGDLKTQTKASTGCGGCSALTKQVLDSELASLGFEVN
NDLCEHFAYSRQELTDIIRVKKITTFDQLLENYGSGLGCEICKPTAGSILASYWNEYVLEDTHLELQDSNDLYLGNIQKN
GTYSVVPRIAGGEITPDKLIVLGEVAKKFDLYTKITGGQRIDLFGAQLHELPVIWQELIDAGFETGHAYGKSLRTVKSCV
GNTWCRFGVDDSMTLALNLENRYKGLRSPHKIKFAVSGCTRECAEAQSKDIGLISTENGWNMYVCGNGGMRPRHGDLFAS
GLDEQTLIKYIDRFLMFYVRTADRLQRTSVWMENMEGGLDYLREVVIEDKLNLADELEQEMENNIKNYQCEWKTTLQNPE
KLKRFKHFINSDDTDNQLAFELEREQRIPAQQTIKVLELTA
>Mature_841_residues
MNKQRIVVVGNGMVGHKFIDTLITDESVDYDIITFSEESRLAYDRVQLSGFFSGKTADDLALTDATYYQENGINFVLNDK
VVKLDTKNNQVVTSSGRIESYDKLVLATGSFPFVPPISGNDKEHCHVYRTIDDLEDITASGKSSKVGVVVGGGLLGLEAA
NALVNLGLETHVVEFAPRLMAVQLDDGGGQLLRRKIEALGVQVHTEKATTEIVDGDNCRYRMNFADGSFLETDTIVFSAG
IRPQDELARQFNLTIGERGGIAINNQCQTNVANIYAIGECALWDNRIFGLVAPGYQMAKVAAAHIKGESLAFTGADMSTK
LKLLGIDVASIGEVHGQTPGAQSYTYHDEIEQVYKRLIVSEDGKKIVGAVLVGDAEAYGTLLQLLLNDMTLPENPAVLIL
PSTGEEQAAGFGVEALPETATICSCFDVSKGDLKSAVIAGCCTMGDLKTQTKASTGCGGCSALTKQVLDSELASLGFEVN
NDLCEHFAYSRQELTDIIRVKKITTFDQLLENYGSGLGCEICKPTAGSILASYWNEYVLEDTHLELQDSNDLYLGNIQKN
GTYSVVPRIAGGEITPDKLIVLGEVAKKFDLYTKITGGQRIDLFGAQLHELPVIWQELIDAGFETGHAYGKSLRTVKSCV
GNTWCRFGVDDSMTLALNLENRYKGLRSPHKIKFAVSGCTRECAEAQSKDIGLISTENGWNMYVCGNGGMRPRHGDLFAS
GLDEQTLIKYIDRFLMFYVRTADRLQRTSVWMENMEGGLDYLREVVIEDKLNLADELEQEMENNIKNYQCEWKTTLQNPE
KLKRFKHFINSDDTDNQLAFELEREQRIPAQQTIKVLELTA

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=319, Percent_Identity=26.9592476489028, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI65787454, Length=319, Percent_Identity=26.9592476489028, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI226437568, Length=319, Percent_Identity=26.9592476489028, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI4757732, Length=271, Percent_Identity=26.9372693726937, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI22202629, Length=271, Percent_Identity=26.9372693726937, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789765, Length=832, Percent_Identity=59.375, Blast_Score=1028, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=386, Percent_Identity=24.8704663212435, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788892, Length=283, Percent_Identity=24.3816254416961, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=319, Percent_Identity=26.0188087774295, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639257, Length=282, Percent_Identity=26.5957446808511, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281359715, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281359713, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639250, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18543267, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639252, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24585130, Length=283, Percent_Identity=24.7349823321555, Blast_Score=80, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 92521; Mature: 92521

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQRIVVVGNGMVGHKFIDTLITDESVDYDIITFSEESRLAYDRVQLSGFFSGKTADDL
CCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHEEEEEEECCCCCCCE
ALTDATYYQENGINFVLNDKVVKLDTKNNQVVTSSGRIESYDKLVLATGSFPFVPPISGN
EECCCCEEECCCCEEEECCEEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DKEHCHVYRTIDDLEDITASGKSSKVGVVVGGGLLGLEAANALVNLGLETHVVEFAPRLM
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCEEE
AVQLDDGGGQLLRRKIEALGVQVHTEKATTEIVDGDNCRYRMNFADGSFLETDTIVFSAG
EEEECCCCHHHHHHHHHHHCEEEECCCCCEEEECCCCCEEEEEECCCCEEECCEEEEECC
IRPQDELARQFNLTIGERGGIAINNQCQTNVANIYAIGECALWDNRIFGLVAPGYQMAKV
CCCHHHHHHHHCCEEECCCCEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCCHHHHHH
AAAHIKGESLAFTGADMSTKLKLLGIDVASIGEVHGQTPGAQSYTYHDEIEQVYKRLIVS
HHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
EDGKKIVGAVLVGDAEAYGTLLQLLLNDMTLPENPAVLILPSTGEEQAAGFGVEALPETA
CCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCH
TICSCFDVSKGDLKSAVIAGCCTMGDLKTQTKASTGCGGCSALTKQVLDSELASLGFEVN
HEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NDLCEHFAYSRQELTDIIRVKKITTFDQLLENYGSGLGCEICKPTAGSILASYWNEYVLE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHEEE
DTHLELQDSNDLYLGNIQKNGTYSVVPRIAGGEITPDKLIVLGEVAKKFDLYTKITGGQR
CCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEECCCCE
IDLFGAQLHELPVIWQELIDAGFETGHAYGKSLRTVKSCVGNTWCRFGVDDSMTLALNLE
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEH
NRYKGLRSPHKIKFAVSGCTRECAEAQSKDIGLISTENGWNMYVCGNGGMRPRHGDLFAS
HHHHCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHC
GLDEQTLIKYIDRFLMFYVRTADRLQRTSVWMENMEGGLDYLREVVIEDKLNLADELEQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
MENNIKNYQCEWKTTLQNPEKLKRFKHFINSDDTDNQLAFELEREQRIPAQQTIKVLELT
HHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCHHHCEEEEEEC
A
C
>Mature Secondary Structure
MNKQRIVVVGNGMVGHKFIDTLITDESVDYDIITFSEESRLAYDRVQLSGFFSGKTADDL
CCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHEEEEEEECCCCCCCE
ALTDATYYQENGINFVLNDKVVKLDTKNNQVVTSSGRIESYDKLVLATGSFPFVPPISGN
EECCCCEEECCCCEEEECCEEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DKEHCHVYRTIDDLEDITASGKSSKVGVVVGGGLLGLEAANALVNLGLETHVVEFAPRLM
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCEEE
AVQLDDGGGQLLRRKIEALGVQVHTEKATTEIVDGDNCRYRMNFADGSFLETDTIVFSAG
EEEECCCCHHHHHHHHHHHCEEEECCCCCEEEECCCCCEEEEEECCCCEEECCEEEEECC
IRPQDELARQFNLTIGERGGIAINNQCQTNVANIYAIGECALWDNRIFGLVAPGYQMAKV
CCCHHHHHHHHCCEEECCCCEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCCHHHHHH
AAAHIKGESLAFTGADMSTKLKLLGIDVASIGEVHGQTPGAQSYTYHDEIEQVYKRLIVS
HHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
EDGKKIVGAVLVGDAEAYGTLLQLLLNDMTLPENPAVLILPSTGEEQAAGFGVEALPETA
CCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCH
TICSCFDVSKGDLKSAVIAGCCTMGDLKTQTKASTGCGGCSALTKQVLDSELASLGFEVN
HEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NDLCEHFAYSRQELTDIIRVKKITTFDQLLENYGSGLGCEICKPTAGSILASYWNEYVLE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHEEE
DTHLELQDSNDLYLGNIQKNGTYSVVPRIAGGEITPDKLIVLGEVAKKFDLYTKITGGQR
CCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEECCCCE
IDLFGAQLHELPVIWQELIDAGFETGHAYGKSLRTVKSCVGNTWCRFGVDDSMTLALNLE
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEH
NRYKGLRSPHKIKFAVSGCTRECAEAQSKDIGLISTENGWNMYVCGNGGMRPRHGDLFAS
HHHHCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHC
GLDEQTLIKYIDRFLMFYVRTADRLQRTSVWMENMEGGLDYLREVVIEDKLNLADELEQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
MENNIKNYQCEWKTTLQNPEKLKRFKHFINSDDTDNQLAFELEREQRIPAQQTIKVLELT
HHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCHHHCEEEEEEC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]