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The map label for this gene is mrcB [H]

Identifier: 119944369

GI number: 119944369

Start: 777438

End: 779630

Strand: Direct

Name: mrcB [H]

Synonym: Ping_0596

Alternate gene names: 119944369

Gene position: 777438-779630 (Clockwise)

Preceding gene: 119944368

Following gene: 119944370

Centisome position: 17.05

GC content: 41.95

Gene sequence:

>2193_bases
ATGTCTAAAAAGAGCAAAACACCTGAGGTTGTTGAAACAAGCAAACAACCTAAATCTAAAGCTGGACCTAAACCTAAACC
TAAACCTAAACCTAAACCTAAATCTCAAAATAAAAAATCTTTGAAACAGTTATTTTTAAGCTTTTTATGGTCTTTTTTTT
GGAAAGGCAGTATCGCTTTTATTGCCCTGATGTTTATCGCCGGTATCTATTTTGATAAAAAGATTGAGCGTAAATTAGAC
GGGTCAACGTGGACGTTACCGGCACAGGTTTATGCCCGGCCTCTCTTATTAACCAGCAGCAGCCAGCTGTCGAAACAAGC
CTTAATAGCCGAGCTTAAGTTATTAAACTATCGTCCGGTACGAAACGTTCAGGCAAGTGGGCAGTTTGCGGTTAACAAAC
AGCAGGTGCTGATTTATAAACGCGCTTTTAATTTTCCGGATCGTTATGAAAGTGCGAAGCTGATTACGGTTAACTTTTCA
GGTGATTGGATTCAGTCGATCAGTGGTAAAAATCAGCAGAATATAAACTCAGTGCGTCTGGATCCCTTATTATTAGAGCG
GCTTGCTTCGAATAATATTGAAGATCGTGTTTTTATTCCTTTTGAGCAAATTCCCGATCTATTGGTTAATACTTTGTTAT
TGATGGAGGATCGTAATTTTTATCACCATCAGGGGATCTCGCCGATAGCTATTCTCCGTGCATTTATCGTTAACGTTAAG
GCCGGGAAAACCTTACAGGGGGGCAGTACCCTTACCCAGCAGTTGGCCAAGAATCTTTTTTTGAACGAAGATCGCAGTTT
GTGGCGTAAATTTCAAGAAGCTTATATCGCGTTGCTAATCGATTATAAATACAGTAAAGATAAAATTTTGGAAGCCTATT
TAAACGAAGTTTATCTGGCTCAGAATGGTAGCTCTGGTATCTATGGGATTGAGCTTGCCAGTGATTATTATTTTGCCCGT
CCTATTAATGAATTACGGATTGAACAAATTGCCCTGCTGGTGGCTATTATCAAAGGCCCTTCCTATTATAACCCTCACCG
CAATCCGCAGCGGGCATTAGCACGTCGCGATCTGGTTTTGCGTACGATGCTTGAATATAACCTGATTGGTGTTCGCGACT
ATCAATATGCAGTACGGGGTGAGCTTAATTTAAGCGCAATAGGTGCTTTAAATCATCGAAAAAATCCCGCCTTTGTCAGT
TTGCTGCGGCGAGAGTTGCAGGCGAATGTTACTAATAAAGTACGTAAACGTAACGGTTTGTTTATTTTTACTTCGATCGA
TCCCTTCGTGCAAAAAAACGCTGAGCTGGCCGTTTCGCAGGGCATTGATGGTCTTGAAAAAAAAGGCCAATTGATGCTAC
AAGGCGCGATGGTGGTGAGCGATCGCAAGCATGCTGAGGTACTGGGTTTAGTTTCAGGAAAAGAGGTGAAATTTAGTGGC
TTTAACCGCGCTTTGGATGCCAAAAGGCCGATCGGATCGTTGGTTAAACCCGTTGTTTATTTAACGGCGTTGGACGGGGG
ATTTGATCTGGATGATAGATTAAATGATAAAGAGATTCTCCTGACAAATGGTTCCGGTGAGCACTGGAGTCCAAAAAATT
ACGACCGTAAATTCCGAGGAGATGTGAGCCTCGAGGATGCCTTAATTTACTCTCTGAATGTACCGACGGTGAATCTAGGG
ATGCAGGTGGGTCTTAACAATGTTATTAATAGCTTGTATAAAATGGGGGTGACGGAAAAAATCAGCGCTTACCCCTCTTT
GGTTTTAGGGAGTGTGGCCCTTTCCCCTTTCCAGGTCGCACAACTGTATCAACCCATCACTATGTCCGGCGTTTATCAAC
CATTAACAATTATTCGCTCAATCGTTTCAAAAGAGGGACAGTTGCTGTATCAACGTCCGGTTCGGCGTAAAAAAATATTC
TCTGAAAAGGCGGTCACAAAATTAACCAATACAATGCATAAAGTGACCACACAAGGCACTGCCCGCAGTTTAACCTGGCG
CAATAAGGGGCAATACTTTTCCGGTAAGACAGGCACCACCAACAATTTATATGACAGCTGGTTTGTTGGTTTCGATGCCG
AGCAGGTGGTGACAACATGGGTTGGAAGGGATGATAATAAAAGTGCACAATTAACCGGTAGTAGTGGAGCATTGATTTTA
TTTTCGGATTATATGAATAATAAAAAAAGATAG

Upstream 100 bases:

>100_bases
ATCCGCTGGTACGGTCCATTATTGGTCGGATAAAAGGTTTGAAGCCGTAGCCGCAGCCGCAAAAAGTGGATAGATCAAAA
ATAAAACAGTTGGGAAGTTG

Downstream 100 bases:

>100_bases
TCATAGTAATTCTGACTATAATGGCTGCGATACGTAACAATGGCTTGTTATAAAGAAGTGTGCCGTTTTATATTTTTTGG
TAAACTTTTAAAGCAAGCAA

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 730; Mature: 729

Protein sequence:

>730_residues
MSKKSKTPEVVETSKQPKSKAGPKPKPKPKPKPKSQNKKSLKQLFLSFLWSFFWKGSIAFIALMFIAGIYFDKKIERKLD
GSTWTLPAQVYARPLLLTSSSQLSKQALIAELKLLNYRPVRNVQASGQFAVNKQQVLIYKRAFNFPDRYESAKLITVNFS
GDWIQSISGKNQQNINSVRLDPLLLERLASNNIEDRVFIPFEQIPDLLVNTLLLMEDRNFYHHQGISPIAILRAFIVNVK
AGKTLQGGSTLTQQLAKNLFLNEDRSLWRKFQEAYIALLIDYKYSKDKILEAYLNEVYLAQNGSSGIYGIELASDYYFAR
PINELRIEQIALLVAIIKGPSYYNPHRNPQRALARRDLVLRTMLEYNLIGVRDYQYAVRGELNLSAIGALNHRKNPAFVS
LLRRELQANVTNKVRKRNGLFIFTSIDPFVQKNAELAVSQGIDGLEKKGQLMLQGAMVVSDRKHAEVLGLVSGKEVKFSG
FNRALDAKRPIGSLVKPVVYLTALDGGFDLDDRLNDKEILLTNGSGEHWSPKNYDRKFRGDVSLEDALIYSLNVPTVNLG
MQVGLNNVINSLYKMGVTEKISAYPSLVLGSVALSPFQVAQLYQPITMSGVYQPLTIIRSIVSKEGQLLYQRPVRRKKIF
SEKAVTKLTNTMHKVTTQGTARSLTWRNKGQYFSGKTGTTNNLYDSWFVGFDAEQVVTTWVGRDDNKSAQLTGSSGALIL
FSDYMNNKKR

Sequences:

>Translated_730_residues
MSKKSKTPEVVETSKQPKSKAGPKPKPKPKPKPKSQNKKSLKQLFLSFLWSFFWKGSIAFIALMFIAGIYFDKKIERKLD
GSTWTLPAQVYARPLLLTSSSQLSKQALIAELKLLNYRPVRNVQASGQFAVNKQQVLIYKRAFNFPDRYESAKLITVNFS
GDWIQSISGKNQQNINSVRLDPLLLERLASNNIEDRVFIPFEQIPDLLVNTLLLMEDRNFYHHQGISPIAILRAFIVNVK
AGKTLQGGSTLTQQLAKNLFLNEDRSLWRKFQEAYIALLIDYKYSKDKILEAYLNEVYLAQNGSSGIYGIELASDYYFAR
PINELRIEQIALLVAIIKGPSYYNPHRNPQRALARRDLVLRTMLEYNLIGVRDYQYAVRGELNLSAIGALNHRKNPAFVS
LLRRELQANVTNKVRKRNGLFIFTSIDPFVQKNAELAVSQGIDGLEKKGQLMLQGAMVVSDRKHAEVLGLVSGKEVKFSG
FNRALDAKRPIGSLVKPVVYLTALDGGFDLDDRLNDKEILLTNGSGEHWSPKNYDRKFRGDVSLEDALIYSLNVPTVNLG
MQVGLNNVINSLYKMGVTEKISAYPSLVLGSVALSPFQVAQLYQPITMSGVYQPLTIIRSIVSKEGQLLYQRPVRRKKIF
SEKAVTKLTNTMHKVTTQGTARSLTWRNKGQYFSGKTGTTNNLYDSWFVGFDAEQVVTTWVGRDDNKSAQLTGSSGALIL
FSDYMNNKKR
>Mature_729_residues
SKKSKTPEVVETSKQPKSKAGPKPKPKPKPKPKSQNKKSLKQLFLSFLWSFFWKGSIAFIALMFIAGIYFDKKIERKLDG
STWTLPAQVYARPLLLTSSSQLSKQALIAELKLLNYRPVRNVQASGQFAVNKQQVLIYKRAFNFPDRYESAKLITVNFSG
DWIQSISGKNQQNINSVRLDPLLLERLASNNIEDRVFIPFEQIPDLLVNTLLLMEDRNFYHHQGISPIAILRAFIVNVKA
GKTLQGGSTLTQQLAKNLFLNEDRSLWRKFQEAYIALLIDYKYSKDKILEAYLNEVYLAQNGSSGIYGIELASDYYFARP
INELRIEQIALLVAIIKGPSYYNPHRNPQRALARRDLVLRTMLEYNLIGVRDYQYAVRGELNLSAIGALNHRKNPAFVSL
LRRELQANVTNKVRKRNGLFIFTSIDPFVQKNAELAVSQGIDGLEKKGQLMLQGAMVVSDRKHAEVLGLVSGKEVKFSGF
NRALDAKRPIGSLVKPVVYLTALDGGFDLDDRLNDKEILLTNGSGEHWSPKNYDRKFRGDVSLEDALIYSLNVPTVNLGM
QVGLNNVINSLYKMGVTEKISAYPSLVLGSVALSPFQVAQLYQPITMSGVYQPLTIIRSIVSKEGQLLYQRPVRRKKIFS
EKAVTKLTNTMHKVTTQGTARSLTWRNKGQYFSGKTGTTNNLYDSWFVGFDAEQVVTTWVGRDDNKSAQLTGSSGALILF
SDYMNNKKR

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=696, Percent_Identity=42.816091954023, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI87082258, Length=257, Percent_Identity=34.2412451361868, Blast_Score=152, Evalue=9e-38,
Organism=Escherichia coli, GI1788867, Length=555, Percent_Identity=25.5855855855856, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789601, Length=173, Percent_Identity=33.5260115606936, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 82410; Mature: 82279

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKSKTPEVVETSKQPKSKAGPKPKPKPKPKPKSQNKKSLKQLFLSFLWSFFWKGSIAF
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
IALMFIAGIYFDKKIERKLDGSTWTLPAQVYARPLLLTSSSQLSKQALIAELKLLNYRPV
HHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCEEEEECCHHHHHHHHHHHHHHHCCCCC
RNVQASGQFAVNKQQVLIYKRAFNFPDRYESAKLITVNFSGDWIQSISGKNQQNINSVRL
CCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCEEC
DPLLLERLASNNIEDRVFIPFEQIPDLLVNTLLLMEDRNFYHHQGISPIAILRAFIVNVK
CHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCC
AGKTLQGGSTLTQQLAKNLFLNEDRSLWRKFQEAYIALLIDYKYSKDKILEAYLNEVYLA
CCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHEEEE
QNGSSGIYGIELASDYYFARPINELRIEQIALLVAIIKGPSYYNPHRNPQRALARRDLVL
ECCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
RTMLEYNLIGVRDYQYAVRGELNLSAIGALNHRKNPAFVSLLRRELQANVTNKVRKRNGL
HHHHHHCEEEEECCCEEEECCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
FIFTSIDPFVQKNAELAVSQGIDGLEKKGQLMLQGAMVVSDRKHAEVLGLVSGKEVKFSG
EEEECCCHHHHCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCCHHEEEEECCCEEEECC
FNRALDAKRPIGSLVKPVVYLTALDGGFDLDDRLNDKEILLTNGSGEHWSPKNYDRKFRG
CCHHHCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHCC
DVSLEDALIYSLNVPTVNLGMQVGLNNVINSLYKMGVTEKISAYPSLVLGSVALSPFQVA
CCCCCCEEEEEECCCEEECCHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
QLYQPITMSGVYQPLTIIRSIVSKEGQLLYQRPVRRKKIFSEKAVTKLTNTMHKVTTQGT
HHHCCHHHHCCHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARSLTWRNKGQYFSGKTGTTNNLYDSWFVGFDAEQVVTTWVGRDDNKSAQLTGSSGALIL
CCEEEECCCCCEECCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCEEEECCCCEEEE
FSDYMNNKKR
EECCCCCCCC
>Mature Secondary Structure 
SKKSKTPEVVETSKQPKSKAGPKPKPKPKPKPKSQNKKSLKQLFLSFLWSFFWKGSIAF
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
IALMFIAGIYFDKKIERKLDGSTWTLPAQVYARPLLLTSSSQLSKQALIAELKLLNYRPV
HHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCEEEEECCHHHHHHHHHHHHHHHCCCCC
RNVQASGQFAVNKQQVLIYKRAFNFPDRYESAKLITVNFSGDWIQSISGKNQQNINSVRL
CCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCEEC
DPLLLERLASNNIEDRVFIPFEQIPDLLVNTLLLMEDRNFYHHQGISPIAILRAFIVNVK
CHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCC
AGKTLQGGSTLTQQLAKNLFLNEDRSLWRKFQEAYIALLIDYKYSKDKILEAYLNEVYLA
CCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHEEEE
QNGSSGIYGIELASDYYFARPINELRIEQIALLVAIIKGPSYYNPHRNPQRALARRDLVL
ECCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
RTMLEYNLIGVRDYQYAVRGELNLSAIGALNHRKNPAFVSLLRRELQANVTNKVRKRNGL
HHHHHHCEEEEECCCEEEECCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
FIFTSIDPFVQKNAELAVSQGIDGLEKKGQLMLQGAMVVSDRKHAEVLGLVSGKEVKFSG
EEEECCCHHHHCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCCHHEEEEECCCEEEECC
FNRALDAKRPIGSLVKPVVYLTALDGGFDLDDRLNDKEILLTNGSGEHWSPKNYDRKFRG
CCHHHCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHCC
DVSLEDALIYSLNVPTVNLGMQVGLNNVINSLYKMGVTEKISAYPSLVLGSVALSPFQVA
CCCCCCEEEEEECCCEEECCHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
QLYQPITMSGVYQPLTIIRSIVSKEGQLLYQRPVRRKKIFSEKAVTKLTNTMHKVTTQGT
HHHCCHHHHCCHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARSLTWRNKGQYFSGKTGTTNNLYDSWFVGFDAEQVVTTWVGRDDNKSAQLTGSSGALIL
CCEEEECCCCCEECCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCEEEECCCCEEEE
FSDYMNNKKR
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]