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The map label for this gene is pfkA [H]

Identifier: 119944364

GI number: 119944364

Start: 773058

End: 774020

Strand: Direct

Name: pfkA [H]

Synonym: Ping_0591

Alternate gene names: 119944364

Gene position: 773058-774020 (Clockwise)

Preceding gene: 119944363

Following gene: 119944365

Centisome position: 16.95

GC content: 41.85

Gene sequence:

>963_bases
ATGATTAAAAGAATAGGGGTTTTAACAAGTGGAGGCGATGCACCCGGTATGAATGCGGCAATCCGTGCCGTAGTACGTGA
AGGCTTACATCTTGGTTTAGAAGTCTATGGTATCTTTGATGGTTATGAAGGGTTGCATCAAGGTAAGATTAAGCCACTCG
ATCGTAATTCTGTTTCTGATGTCATTAACCGTGGCGGTACTTTTTTAGGCTCGGCACGTTTCCCTGAATTTAAACAACAA
AAAACCCGTGAAGAAGCCATTGAAAATCTTAAAAAATACGGTATTGATGCCTTAGTGGTTATTGGTGGTGATGGTTCATA
CATGGGGGCTAAAAAACTCACAGAAATGGGTTACCCCTGTATCGGTATTCCAGGCACTATTGATAATGATATTCCAGGTA
CAACTTATACTATCGGTTTTGATACTGCACTAAATATCGTTGTTGAAGCAATTGACCGTCTACGTGATACCTCTGCATCA
CACAAACGTATCTCCATTGTTGAAATCATGGGTCGTTACTGTGGTGATTTAACCTTAGGTGCCGCTGTCGCGGGCGGTGC
TGAATTTGTTGTAATTCCAGAAAAAGGGTATAGCGAAGCTGAGTTACTGGGGCAAATCAAAGCCGGTATTGCAAAAGGTA
AAACCCATATGATTATCGCCATGTGTGAGCACGTAACAAAGGTTGATAAACTTGCGGAGCATATCCAAGCTGAAACCGGC
CATGATACACGTGCAACCATTTTAGGTCATATCCAACGTGGCGGTACACCGACAGCCCAAGATCGCATTTTGGCGAGTCG
TATGGGATCTTTTGCTGTTAAGCTGTTATTAGAAGATCAAGGCGGTCGTTGCATTGGCGTCGTGAATTCACAAATGGTTC
ACTATGATATTATTGATTGTATCAACAATATGAAACGACCTTTTAATCAAGAATTATTTGATCTGTCGGACTCTTTATTC
TGA

Upstream 100 bases:

>100_bases
GGAGTGAGTGTTTAAAATAACTAAAGGGTTGTTTTTATAAGTTGTTGAGCTCACTTTAACCTAAATTGAAAGACTATTTA
TTCGTATATTGGAGATTGTC

Downstream 100 bases:

>100_bases
GCCAAAATTCCATACTTGAATGATCAACAGCCTGCTTATTATTATATTTCCAATTAAACAGAGGAAATGTAACATTAAAG
CTTATTTTGGAGAATATATA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKRIGVLTSGGDAPGMNAAIRAVVREGLHLGLEVYGIFDGYEGLHQGKIKPLDRNSVSDVINRGGTFLGSARFPEFKQQ
KTREEAIENLKKYGIDALVVIGGDGSYMGAKKLTEMGYPCIGIPGTIDNDIPGTTYTIGFDTALNIVVEAIDRLRDTSAS
HKRISIVEIMGRYCGDLTLGAAVAGGAEFVVIPEKGYSEAELLGQIKAGIAKGKTHMIIAMCEHVTKVDKLAEHIQAETG
HDTRATILGHIQRGGTPTAQDRILASRMGSFAVKLLLEDQGGRCIGVVNSQMVHYDIIDCINNMKRPFNQELFDLSDSLF

Sequences:

>Translated_320_residues
MIKRIGVLTSGGDAPGMNAAIRAVVREGLHLGLEVYGIFDGYEGLHQGKIKPLDRNSVSDVINRGGTFLGSARFPEFKQQ
KTREEAIENLKKYGIDALVVIGGDGSYMGAKKLTEMGYPCIGIPGTIDNDIPGTTYTIGFDTALNIVVEAIDRLRDTSAS
HKRISIVEIMGRYCGDLTLGAAVAGGAEFVVIPEKGYSEAELLGQIKAGIAKGKTHMIIAMCEHVTKVDKLAEHIQAETG
HDTRATILGHIQRGGTPTAQDRILASRMGSFAVKLLLEDQGGRCIGVVNSQMVHYDIIDCINNMKRPFNQELFDLSDSLF
>Mature_320_residues
MIKRIGVLTSGGDAPGMNAAIRAVVREGLHLGLEVYGIFDGYEGLHQGKIKPLDRNSVSDVINRGGTFLGSARFPEFKQQ
KTREEAIENLKKYGIDALVVIGGDGSYMGAKKLTEMGYPCIGIPGTIDNDIPGTTYTIGFDTALNIVVEAIDRLRDTSAS
HKRISIVEIMGRYCGDLTLGAAVAGGAEFVVIPEKGYSEAELLGQIKAGIAKGKTHMIIAMCEHVTKVDKLAEHIQAETG
HDTRATILGHIQRGGTPTAQDRILASRMGSFAVKLLLEDQGGRCIGVVNSQMVHYDIIDCINNMKRPFNQELFDLSDSLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=339, Percent_Identity=40.7079646017699, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI48762920, Length=363, Percent_Identity=39.3939393939394, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI266453768, Length=355, Percent_Identity=38.8732394366197, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI266453748, Length=355, Percent_Identity=38.8732394366197, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI4505749, Length=355, Percent_Identity=38.8732394366197, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI266453619, Length=355, Percent_Identity=38.8732394366197, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=69.6875, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25147584, Length=339, Percent_Identity=37.7581120943953, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=320, Percent_Identity=38.125, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=32.4137931034483, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=38.0813953488372, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323861, Length=352, Percent_Identity=36.0795454545455, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28573326, Length=341, Percent_Identity=38.41642228739, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24652337, Length=341, Percent_Identity=38.41642228739, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI17647809, Length=337, Percent_Identity=38.8724035608309, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34565; Mature: 34565

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRIGVLTSGGDAPGMNAAIRAVVREGLHLGLEVYGIFDGYEGLHQGKIKPLDRNSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCCHHHH
VINRGGTFLGSARFPEFKQQKTREEAIENLKKYGIDALVVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCE
IGIPGTIDNDIPGTTYTIGFDTALNIVVEAIDRLRDTSASHKRISIVEIMGRYCGDLTLG
EECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHH
AAVAGGAEFVVIPEKGYSEAELLGQIKAGIAKGKTHMIIAMCEHVTKVDKLAEHIQAETG
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HDTRATILGHIQRGGTPTAQDRILASRMGSFAVKLLLEDQGGRCIGVVNSQMVHYDIIDC
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCHHHHHHHHH
INNMKRPFNQELFDLSDSLF
HHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIKRIGVLTSGGDAPGMNAAIRAVVREGLHLGLEVYGIFDGYEGLHQGKIKPLDRNSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCCHHHH
VINRGGTFLGSARFPEFKQQKTREEAIENLKKYGIDALVVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCE
IGIPGTIDNDIPGTTYTIGFDTALNIVVEAIDRLRDTSASHKRISIVEIMGRYCGDLTLG
EECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHH
AAVAGGAEFVVIPEKGYSEAELLGQIKAGIAKGKTHMIIAMCEHVTKVDKLAEHIQAETG
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HDTRATILGHIQRGGTPTAQDRILASRMGSFAVKLLLEDQGGRCIGVVNSQMVHYDIIDC
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCHHHHHHHHH
INNMKRPFNQELFDLSDSLF
HHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA