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The map label for this gene is dppA [H]

Identifier: 119944335

GI number: 119944335

Start: 729220

End: 730776

Strand: Direct

Name: dppA [H]

Synonym: Ping_0562

Alternate gene names: 119944335

Gene position: 729220-730776 (Clockwise)

Preceding gene: 119944334

Following gene: 119944336

Centisome position: 15.99

GC content: 42.0

Gene sequence:

>1557_bases
ATGAAATTTTTCAAAACCAAACTAACGGTTGCTTTGCTGGCAGCAGGATTAAGCTTTAGTGTTGCAGCAGCAGATATTAC
TGTTGCTTATGATGCTGATCCGGTATCACTTGACCCGCATGAGCAACTTTCAGGTGGAACGCTGCAGATGTCCCATATGC
TTTTTGATCCGCTTATTCGTTTCACTCAAGAAATGGACTTTGAACCCCGTTTAGCTTCAAAATGGGAACGTATTGATGCG
ACCACATTCCGCTTTAATCTGCGTAAAGGGGTTAAATTCCACTCCGGCAATACGATGACTGCGGACGATGTTATTTGGAC
ATTTAATCGTCTGAAAAACTCTGCTGACTTTAAAGGCGTTTTTTCACCTTATGAGAAAATGATTAAAATCGATGATTACA
CGGTAGAGATCAAGTCAACTGAACCTTACCCGTTAGTACTGCAAACGGCGACCTATATCTTCCCGATGGACAGAAAATTC
TATACTGGCGAAATGGACGGTAAAGATAAAAGTGAAATTTTAAAACATGGTGGTTCTTTTGCGTCAACGCACGTTTCAGG
CACAGGTCCCTATACAATAACAACACGTGAACAGGGCATTAAAGTGGTATTTGATCGTTTTGATGATTATTGGGATACAA
ACTCTCCCGGTAACGTTGATAAACTGACACTGGTACCGATTAAAGAAGATGCAACACGTGTTGCAGCCCTTCTTTCTGGT
GATGTCGATATGATCTATCCGGTGGCACCAAACGATCAGAAACGTGTTGCTGATACTGAAGGTCTTAAACTAACGACTGT
TCCCGGCTCTCGCATTATCACTTTCCAAATGAATCAGAAAAGTAATCCGGCGCTTAAAGATGTACGCGTTCGCCAGGCGA
TTGTCCATGCCATTAATAATGACGGTATTGTTAAAAAGATCATGAAAGATTTTGCAACGACTGCTGGCCAACAAGGACCT
GCTGGTTACGCGGGTTATGATGAAGCCTTAGTGCCTCGTTTTGATGTGGCAAAAGCGAAGAAATTAATGGAAGAAGCGGG
TTACTCTAAAGATAAACCCTTGCAGATTTCTATGATGGCACCCAACAACCGTTATGTGAAGGATGCTCAAATCGCACAGG
CAACTGCCGGTATGCTAGCTAAAATTTACATTAATGTAGACTTGAAAACACTGCCTAAAGCGCAATACTGGCCTGAATTT
GATGTTTGTGCTTCGGACATGATGATGATCGGTTGGCATTCAGATACAGAAGATTCAGCTAACTTTTCTGAATTCCTGAT
GATGACGCGTGATGCTGATGCGGGTAAAGGTGCTTATAACTGCGGGAATTACTCTAATCCAGAAGTTGATCGTTTAGTTA
ATGCTGCCAATGCTGAAACAGATCCTGCTAAACGTGCGACTATGTTACAAAAGGTTGAGCAAATATTGTACAAAGATGCG
GCATTTGTACCTCTTCATTGGCAGAACTTGGCATGGGGATCAAAATCTAACCTTCAGATTGAACCCATAGTTAACTCCAT
GAACTTCCCATACTTCGGTGACCTTATTGTTAAATAG

Upstream 100 bases:

>100_bases
AGGAATTTAAAATAGTGTAAGCATCATGTTAGCTGTGTGACTACATTCTAAAACAACCAAAACCAACGGGAAATTAGTCC
CGGCATCAAGGAGTTATGTA

Downstream 100 bases:

>100_bases
TGAGTTGTTAAATAACGAGTTATTGAATAGGTAAGAAGTAGTTAAGTAACACTTAAAAAGGCCCTATTTATTTAGGGCCT
TAGTCAATATCCAAACCACT

Product: oligopeptide ABC transporter extracellular solute-binding family 5 protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKFFKTKLTVALLAAGLSFSVAAADITVAYDADPVSLDPHEQLSGGTLQMSHMLFDPLIRFTQEMDFEPRLASKWERIDA
TTFRFNLRKGVKFHSGNTMTADDVIWTFNRLKNSADFKGVFSPYEKMIKIDDYTVEIKSTEPYPLVLQTATYIFPMDRKF
YTGEMDGKDKSEILKHGGSFASTHVSGTGPYTITTREQGIKVVFDRFDDYWDTNSPGNVDKLTLVPIKEDATRVAALLSG
DVDMIYPVAPNDQKRVADTEGLKLTTVPGSRIITFQMNQKSNPALKDVRVRQAIVHAINNDGIVKKIMKDFATTAGQQGP
AGYAGYDEALVPRFDVAKAKKLMEEAGYSKDKPLQISMMAPNNRYVKDAQIAQATAGMLAKIYINVDLKTLPKAQYWPEF
DVCASDMMMIGWHSDTEDSANFSEFLMMTRDADAGKGAYNCGNYSNPEVDRLVNAANAETDPAKRATMLQKVEQILYKDA
AFVPLHWQNLAWGSKSNLQIEPIVNSMNFPYFGDLIVK

Sequences:

>Translated_518_residues
MKFFKTKLTVALLAAGLSFSVAAADITVAYDADPVSLDPHEQLSGGTLQMSHMLFDPLIRFTQEMDFEPRLASKWERIDA
TTFRFNLRKGVKFHSGNTMTADDVIWTFNRLKNSADFKGVFSPYEKMIKIDDYTVEIKSTEPYPLVLQTATYIFPMDRKF
YTGEMDGKDKSEILKHGGSFASTHVSGTGPYTITTREQGIKVVFDRFDDYWDTNSPGNVDKLTLVPIKEDATRVAALLSG
DVDMIYPVAPNDQKRVADTEGLKLTTVPGSRIITFQMNQKSNPALKDVRVRQAIVHAINNDGIVKKIMKDFATTAGQQGP
AGYAGYDEALVPRFDVAKAKKLMEEAGYSKDKPLQISMMAPNNRYVKDAQIAQATAGMLAKIYINVDLKTLPKAQYWPEF
DVCASDMMMIGWHSDTEDSANFSEFLMMTRDADAGKGAYNCGNYSNPEVDRLVNAANAETDPAKRATMLQKVEQILYKDA
AFVPLHWQNLAWGSKSNLQIEPIVNSMNFPYFGDLIVK
>Mature_518_residues
MKFFKTKLTVALLAAGLSFSVAAADITVAYDADPVSLDPHEQLSGGTLQMSHMLFDPLIRFTQEMDFEPRLASKWERIDA
TTFRFNLRKGVKFHSGNTMTADDVIWTFNRLKNSADFKGVFSPYEKMIKIDDYTVEIKSTEPYPLVLQTATYIFPMDRKF
YTGEMDGKDKSEILKHGGSFASTHVSGTGPYTITTREQGIKVVFDRFDDYWDTNSPGNVDKLTLVPIKEDATRVAALLSG
DVDMIYPVAPNDQKRVADTEGLKLTTVPGSRIITFQMNQKSNPALKDVRVRQAIVHAINNDGIVKKIMKDFATTAGQQGP
AGYAGYDEALVPRFDVAKAKKLMEEAGYSKDKPLQISMMAPNNRYVKDAQIAQATAGMLAKIYINVDLKTLPKAQYWPEF
DVCASDMMMIGWHSDTEDSANFSEFLMMTRDADAGKGAYNCGNYSNPEVDRLVNAANAETDPAKRATMLQKVEQILYKDA
AFVPLHWQNLAWGSKSNLQIEPIVNSMNFPYFGDLIVK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=501, Percent_Identity=26.5469061876248, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1787495, Length=540, Percent_Identity=24.6296296296296, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787052, Length=503, Percent_Identity=23.8568588469185, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081878, Length=526, Percent_Identity=23.7642585551331, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1787551, Length=447, Percent_Identity=24.8322147651007, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787762, Length=505, Percent_Identity=25.3465346534653, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789397, Length=525, Percent_Identity=23.4285714285714, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789887, Length=538, Percent_Identity=22.3048327137546, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57876; Mature: 57876

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFKTKLTVALLAAGLSFSVAAADITVAYDADPVSLDPHEQLSGGTLQMSHMLFDPLIR
CCHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCEEEHHHHHHHHHHH
FTQEMDFEPRLASKWERIDATTFRFNLRKGVKFHSGNTMTADDVIWTFNRLKNSADFKGV
HHHHCCCCCHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCHH
FSPYEKMIKIDDYTVEIKSTEPYPLVLQTATYIFPMDRKFYTGEMDGKDKSEILKHGGSF
HHHHHHHEEECCEEEEEECCCCCEEEEEEEEEEEECCCEEEECCCCCCCHHHHHHCCCCE
ASTHVSGTGPYTITTREQGIKVVFDRFDDYWDTNSPGNVDKLTLVPIKEDATRVAALLSG
EEEEECCCCCEEEEECCCCHHHEEHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCC
DVDMIYPVAPNDQKRVADTEGLKLTTVPGSRIITFQMNQKSNPALKDVRVRQAIVHAINN
CCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DGIVKKIMKDFATTAGQQGPAGYAGYDEALVPRFDVAKAKKLMEEAGYSKDKPLQISMMA
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
PNNRYVKDAQIAQATAGMLAKIYINVDLKTLPKAQYWPEFDVCASDMMMIGWHSDTEDSA
CCCCEECHHHHHHHHHCEEEEEEEEEEHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCC
NFSEFLMMTRDADAGKGAYNCGNYSNPEVDRLVNAANAETDPAKRATMLQKVEQILYKDA
CHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
AFVPLHWQNLAWGSKSNLQIEPIVNSMNFPYFGDLIVK
CEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEC
>Mature Secondary Structure
MKFFKTKLTVALLAAGLSFSVAAADITVAYDADPVSLDPHEQLSGGTLQMSHMLFDPLIR
CCHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCEEEHHHHHHHHHHH
FTQEMDFEPRLASKWERIDATTFRFNLRKGVKFHSGNTMTADDVIWTFNRLKNSADFKGV
HHHHCCCCCHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCHH
FSPYEKMIKIDDYTVEIKSTEPYPLVLQTATYIFPMDRKFYTGEMDGKDKSEILKHGGSF
HHHHHHHEEECCEEEEEECCCCCEEEEEEEEEEEECCCEEEECCCCCCCHHHHHHCCCCE
ASTHVSGTGPYTITTREQGIKVVFDRFDDYWDTNSPGNVDKLTLVPIKEDATRVAALLSG
EEEEECCCCCEEEEECCCCHHHEEHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCC
DVDMIYPVAPNDQKRVADTEGLKLTTVPGSRIITFQMNQKSNPALKDVRVRQAIVHAINN
CCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DGIVKKIMKDFATTAGQQGPAGYAGYDEALVPRFDVAKAKKLMEEAGYSKDKPLQISMMA
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
PNNRYVKDAQIAQATAGMLAKIYINVDLKTLPKAQYWPEFDVCASDMMMIGWHSDTEDSA
CCCCEECHHHHHHHHHCEEEEEEEEEEHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCC
NFSEFLMMTRDADAGKGAYNCGNYSNPEVDRLVNAANAETDPAKRATMLQKVEQILYKDA
CHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
AFVPLHWQNLAWGSKSNLQIEPIVNSMNFPYFGDLIVK
CEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]