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The map label for this gene is dsbA [H]

Identifier: 119944330

GI number: 119944330

Start: 720182

End: 720823

Strand: Reverse

Name: dsbA [H]

Synonym: Ping_0557

Alternate gene names: 119944330

Gene position: 720823-720182 (Counterclockwise)

Preceding gene: 119944331

Following gene: 119944327

Centisome position: 15.81

GC content: 42.83

Gene sequence:

>642_bases
ATGAATCTTATCAAGCAATTATTTTTGACCCTAATTTTGCTATGCGCCAGCCAGGCTTTTGCAAAACCAGAATTAGGTAA
GGATTACTCAATTTTAAATCCCGCAATGCCTACGCATAGCGGTAAAAAGATTGAGGTTCTAGAATTTTTTTCCTACGACT
GTATTCATTGCTACAACTTTAATCCTTTGATGCAAGCTTGGACAAAGCGCATGCCCGATGATGTTTCTCTGACTTATGTG
CCAGTAGTATACAGAGCCGACATGGAAATACCTGCCCGTGCTTTTTACGCGGTGGAGTACCTCGGTTTACACAACAAATT
GCATGACACCTTATTCGACATCTGGCATAGCAGCAACCCACCTTATGACAAAGCCAGCCTGATCAATGCGCTCACACCCT
ATGGTGTAGACGTTACTAAATTCCAAAATGCCTACAACGCTTACTCTATCAGCCAGGCATTGGAACGCGCCAGGGGATTG
GCATACACCTTGGATATACATATGCAAAAGAACGACTATCACATTCGTAGCACACCAACGCTGATTATCGGTGGCAAATA
TGTCATTTCTGGGGAATCGGCACACACGATTAAACTCCTCTATGCCTTGATCGAAAAAGTTCGCAAGGAAAAAAAGCATT
AA

Upstream 100 bases:

>100_bases
CTGCTGCTGAAATGAATGATGTCGTCACTACATTAAAACAGAATGGTGTGCCCGCCACCCGTGGTGCCCAATAACATTAG
CTTTTATTAAAGGAGTCCAC

Downstream 100 bases:

>100_bases
TTCCGCAGCACAATTGTCCCCGTATTTTAATCACTGGCATATCGTGCGGTATTGGCTTGGAATTGGCATTGGCATTGGCA
TTGGCATTGGCCGAGTACTA

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNLIKQLFLTLILLCASQAFAKPELGKDYSILNPAMPTHSGKKIEVLEFFSYDCIHCYNFNPLMQAWTKRMPDDVSLTYV
PVVYRADMEIPARAFYAVEYLGLHNKLHDTLFDIWHSSNPPYDKASLINALTPYGVDVTKFQNAYNAYSISQALERARGL
AYTLDIHMQKNDYHIRSTPTLIIGGKYVISGESAHTIKLLYALIEKVRKEKKH

Sequences:

>Translated_213_residues
MNLIKQLFLTLILLCASQAFAKPELGKDYSILNPAMPTHSGKKIEVLEFFSYDCIHCYNFNPLMQAWTKRMPDDVSLTYV
PVVYRADMEIPARAFYAVEYLGLHNKLHDTLFDIWHSSNPPYDKASLINALTPYGVDVTKFQNAYNAYSISQALERARGL
AYTLDIHMQKNDYHIRSTPTLIIGGKYVISGESAHTIKLLYALIEKVRKEKKH
>Mature_213_residues
MNLIKQLFLTLILLCASQAFAKPELGKDYSILNPAMPTHSGKKIEVLEFFSYDCIHCYNFNPLMQAWTKRMPDDVSLTYV
PVVYRADMEIPARAFYAVEYLGLHNKLHDTLFDIWHSSNPPYDKASLINALTPYGVDVTKFQNAYNAYSISQALERARGL
AYTLDIHMQKNDYHIRSTPTLIIGGKYVISGESAHTIKLLYALIEKVRKEKKH

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24395; Mature: 24395

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIKQLFLTLILLCASQAFAKPELGKDYSILNPAMPTHSGKKIEVLEFFSYDCIHCYNF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
NPLMQAWTKRMPDDVSLTYVPVVYRADMEIPARAFYAVEYLGLHNKLHDTLFDIWHSSNP
CHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYDKASLINALTPYGVDVTKFQNAYNAYSISQALERARGLAYTLDIHMQKNDYHIRSTPT
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCE
LIIGGKYVISGESAHTIKLLYALIEKVRKEKKH
EEECCEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLIKQLFLTLILLCASQAFAKPELGKDYSILNPAMPTHSGKKIEVLEFFSYDCIHCYNF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
NPLMQAWTKRMPDDVSLTYVPVVYRADMEIPARAFYAVEYLGLHNKLHDTLFDIWHSSNP
CHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYDKASLINALTPYGVDVTKFQNAYNAYSISQALERARGLAYTLDIHMQKNDYHIRSTPT
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCE
LIIGGKYVISGESAHTIKLLYALIEKVRKEKKH
EEECCEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]