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The map label for this gene is appA [H]

Identifier: 119944318

GI number: 119944318

Start: 696206

End: 697843

Strand: Direct

Name: appA [H]

Synonym: Ping_0545

Alternate gene names: 119944318

Gene position: 696206-697843 (Clockwise)

Preceding gene: 119944317

Following gene: 119944319

Centisome position: 15.27

GC content: 40.23

Gene sequence:

>1638_bases
ATGCATAACAAAAAGATTTTAGCTTCTATCATATGCGGCTTATTAATATCTTCAAACAGCGTCGCCTCAGAAGATTTTAA
ACAGGGTGGTACATTGACTGTGCCAATTATTGGTACCGGTTTTGTTGATAACTACAATCCCTATACAACCAAAGACTTAT
TGCACGGTGTGATGTTTGAACCTTTACTGGTTTTTAATAATATGACTGGTAAAACTGAATACCGTTTAGCAAAATCTGCT
GAATATTCCGATGATCTTAAAACTATCACAGTTAAGTTACGTGATAATTTAAAGTGGTCTGACGGCTCGGCATTAACAGC
AACAGATGTCGCCTTTAGCTTTACCATGACTAAGGATACACCGGCCTTTGATCAGAAAGGGATTTGGACTGCAGGTAACC
TGGCCAGTGTCACCGCGACGAATGCAACAACCATTGTTTTTGCCTTAAATGAAGCTGATTCTACTTTTATCTGGAATCTG
GAAAGATACCATATCGTGCCTGAGCAGGTTTGGAGTAAAGCAGAAAATTTAACCACTTTTACTAACCCAGATCCTATTGG
CAGCGGTCCAATGACAGAGGTTACCTATCTTAAAGCGCAGCAGATGGAAATTTGCCGTAACCCTGAATATTACCTTGCAA
ACCGTCCGTATCTGGATTGTATTACTTTCCGTTCTTACAATGATAACTCACAAATTCAGCCTGCCTTAATGAAAGGTGAG
ATTGACTGGGGTTCTAACTTTATTGCCGATGTCGAAAATACCTTTGTCGCAGAGAATCCTAAAAATCATCATTTTTGGTA
CCCGGCTAATGATGCAATCCATCTTTATGTCAACACCAAAGTAAAACCTTTTGATGATCTGAACGTCCGTAAAGCTTTAT
CAATGGCACTGGATCGTGACGAAATTGTTGATATCGCGGCATATGGTTATCCAACGGCTAACTTTAATGTGGGTGGTATT
GGTGAACTGTACGATTCTTATATTGATAAGGAAGTAACAGCTAAATACAAAGACATTACTGAATTTAATCCTGAAAAAGC
AAAGCAGTTGCTTGATGCTGCCGGTTACACCGATCTTAACGACGACGGATTCCGCGACATGCCGGATGGCACGCCAATTG
AATTTGATATCGAAGTGGTAAATGGCTGGACGGATTGGATTCAAGTCGTCCAGATGGTCACTGAGTACTACGAAGAAGTG
GGTATTAAAGCGAATGTTAAAACCGTCGATTGGGCAGTTTATGACTCATCACTGAAAGACGGTAAATACAAAATGGCCAT
TAACTGGTCTTTAGTGGCAACCAATCCGATTCTGGCTTATCAGGAATACTTTGATACTGCACGTATCGGTAAAACCTGGC
ATGCAGGTCACGGTGTCCATTCGGCAAAAATTGATACTCTGATCAATAGCTTTGGTTCAACCAATGATCCTAAAAAGCAG
CAGGCAATACTTAATCAGTTGCAGGAATTCTCTGCGGCAAACTTAGCCTTTATTCCTTTGTTTTCAAACCCTACCTGGTT
CCAATACAACAGCTCAAAAATTGTTGGATGGCCAAGCAAAGAAAATCCATATGTTCAGCCTGTTTGGTATGACGGTGGTA
AACGTAACTTAATTATCAATAATCTTCACCTTAAGTAA

Upstream 100 bases:

>100_bases
TCTTTGTTGTTGTGGGATTAGGTTTCTGTAAAGGCATGGATACCAGATAAGGCTCAGGCAAGCGTTTATGTTGTGATTCA
CTAAATTTAAGGATTCTTTA

Downstream 100 bases:

>100_bases
TTTTTGTTTATAAAAAGAGCCACATTGTGGCTCTTTAATTGTTGGAAAAGATTATGAGTTTTTTATTACGTCGACTTGGT
TTTTATTTTACTGCTTTCTT

Product: oligopeptide ABC transporter extracellular solute-binding family 5 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MHNKKILASIICGLLISSNSVASEDFKQGGTLTVPIIGTGFVDNYNPYTTKDLLHGVMFEPLLVFNNMTGKTEYRLAKSA
EYSDDLKTITVKLRDNLKWSDGSALTATDVAFSFTMTKDTPAFDQKGIWTAGNLASVTATNATTIVFALNEADSTFIWNL
ERYHIVPEQVWSKAENLTTFTNPDPIGSGPMTEVTYLKAQQMEICRNPEYYLANRPYLDCITFRSYNDNSQIQPALMKGE
IDWGSNFIADVENTFVAENPKNHHFWYPANDAIHLYVNTKVKPFDDLNVRKALSMALDRDEIVDIAAYGYPTANFNVGGI
GELYDSYIDKEVTAKYKDITEFNPEKAKQLLDAAGYTDLNDDGFRDMPDGTPIEFDIEVVNGWTDWIQVVQMVTEYYEEV
GIKANVKTVDWAVYDSSLKDGKYKMAINWSLVATNPILAYQEYFDTARIGKTWHAGHGVHSAKIDTLINSFGSTNDPKKQ
QAILNQLQEFSAANLAFIPLFSNPTWFQYNSSKIVGWPSKENPYVQPVWYDGGKRNLIINNLHLK

Sequences:

>Translated_545_residues
MHNKKILASIICGLLISSNSVASEDFKQGGTLTVPIIGTGFVDNYNPYTTKDLLHGVMFEPLLVFNNMTGKTEYRLAKSA
EYSDDLKTITVKLRDNLKWSDGSALTATDVAFSFTMTKDTPAFDQKGIWTAGNLASVTATNATTIVFALNEADSTFIWNL
ERYHIVPEQVWSKAENLTTFTNPDPIGSGPMTEVTYLKAQQMEICRNPEYYLANRPYLDCITFRSYNDNSQIQPALMKGE
IDWGSNFIADVENTFVAENPKNHHFWYPANDAIHLYVNTKVKPFDDLNVRKALSMALDRDEIVDIAAYGYPTANFNVGGI
GELYDSYIDKEVTAKYKDITEFNPEKAKQLLDAAGYTDLNDDGFRDMPDGTPIEFDIEVVNGWTDWIQVVQMVTEYYEEV
GIKANVKTVDWAVYDSSLKDGKYKMAINWSLVATNPILAYQEYFDTARIGKTWHAGHGVHSAKIDTLINSFGSTNDPKKQ
QAILNQLQEFSAANLAFIPLFSNPTWFQYNSSKIVGWPSKENPYVQPVWYDGGKRNLIINNLHLK
>Mature_545_residues
MHNKKILASIICGLLISSNSVASEDFKQGGTLTVPIIGTGFVDNYNPYTTKDLLHGVMFEPLLVFNNMTGKTEYRLAKSA
EYSDDLKTITVKLRDNLKWSDGSALTATDVAFSFTMTKDTPAFDQKGIWTAGNLASVTATNATTIVFALNEADSTFIWNL
ERYHIVPEQVWSKAENLTTFTNPDPIGSGPMTEVTYLKAQQMEICRNPEYYLANRPYLDCITFRSYNDNSQIQPALMKGE
IDWGSNFIADVENTFVAENPKNHHFWYPANDAIHLYVNTKVKPFDDLNVRKALSMALDRDEIVDIAAYGYPTANFNVGGI
GELYDSYIDKEVTAKYKDITEFNPEKAKQLLDAAGYTDLNDDGFRDMPDGTPIEFDIEVVNGWTDWIQVVQMVTEYYEEV
GIKANVKTVDWAVYDSSLKDGKYKMAINWSLVATNPILAYQEYFDTARIGKTWHAGHGVHSAKIDTLINSFGSTNDPKKQ
QAILNQLQEFSAANLAFIPLFSNPTWFQYNSSKIVGWPSKENPYVQPVWYDGGKRNLIINNLHLK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=24.9512670565302, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789887, Length=462, Percent_Identity=23.3766233766234, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082063, Length=568, Percent_Identity=22.3591549295775, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789966, Length=447, Percent_Identity=24.3847874720358, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787762, Length=434, Percent_Identity=21.6589861751152, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787551, Length=382, Percent_Identity=22.7748691099476, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI87081878, Length=309, Percent_Identity=24.5954692556634, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61423; Mature: 61423

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01159 WW_DOMAIN_1 ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNKKILASIICGLLISSNSVASEDFKQGGTLTVPIIGTGFVDNYNPYTTKDLLHGVMFE
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHC
PLLVFNNMTGKTEYRLAKSAEYSDDLKTITVKLRDNLKWSDGSALTATDVAFSFTMTKDT
CEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCC
PAFDQKGIWTAGNLASVTATNATTIVFALNEADSTFIWNLERYHIVPEQVWSKAENLTTF
CCCCCCCCEECCCEEEEEECCCEEEEEEEECCCCEEEEECHHEEECHHHHHHHHHCCEEC
TNPDPIGSGPMTEVTYLKAQQMEICRNPEYYLANRPYLDCITFRSYNDNSQIQPALMKGE
CCCCCCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCCCCCCCEEEECC
IDWGSNFIADVENTFVAENPKNHHFWYPANDAIHLYVNTKVKPFDDLNVRKALSMALDRD
CCCCCCEEECCCCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHCCHH
EIVDIAAYGYPTANFNVGGIGELYDSYIDKEVTAKYKDITEFNPEKAKQLLDAAGYTDLN
HEEEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
DDGFRDMPDGTPIEFDIEVVNGWTDWIQVVQMVTEYYEEVGIKANVKTVDWAVYDSSLKD
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCC
GKYKMAINWSLVATNPILAYQEYFDTARIGKTWHAGHGVHSAKIDTLINSFGSTNDPKKQ
CEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHH
QAILNQLQEFSAANLAFIPLFSNPTWFQYNSSKIVGWPSKENPYVQPVWYDGGKRNLIIN
HHHHHHHHHCCCCCEEEEEEECCCCEEEECCCEEEECCCCCCCEEEEEEECCCCCEEEEE
NLHLK
EEECC
>Mature Secondary Structure
MHNKKILASIICGLLISSNSVASEDFKQGGTLTVPIIGTGFVDNYNPYTTKDLLHGVMFE
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHC
PLLVFNNMTGKTEYRLAKSAEYSDDLKTITVKLRDNLKWSDGSALTATDVAFSFTMTKDT
CEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCC
PAFDQKGIWTAGNLASVTATNATTIVFALNEADSTFIWNLERYHIVPEQVWSKAENLTTF
CCCCCCCCEECCCEEEEEECCCEEEEEEEECCCCEEEEECHHEEECHHHHHHHHHCCEEC
TNPDPIGSGPMTEVTYLKAQQMEICRNPEYYLANRPYLDCITFRSYNDNSQIQPALMKGE
CCCCCCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCCCCCCCEEEECC
IDWGSNFIADVENTFVAENPKNHHFWYPANDAIHLYVNTKVKPFDDLNVRKALSMALDRD
CCCCCCEEECCCCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHCCHH
EIVDIAAYGYPTANFNVGGIGELYDSYIDKEVTAKYKDITEFNPEKAKQLLDAAGYTDLN
HEEEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
DDGFRDMPDGTPIEFDIEVVNGWTDWIQVVQMVTEYYEEVGIKANVKTVDWAVYDSSLKD
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCC
GKYKMAINWSLVATNPILAYQEYFDTARIGKTWHAGHGVHSAKIDTLINSFGSTNDPKKQ
CEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHH
QAILNQLQEFSAANLAFIPLFSNPTWFQYNSSKIVGWPSKENPYVQPVWYDGGKRNLIIN
HHHHHHHHHCCCCCEEEEEEECCCCEEEECCCEEEECCCCCCCEEEEEEECCCCCEEEEE
NLHLK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]