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The map label for this gene is mltD [H]

Identifier: 119944273

GI number: 119944273

Start: 633551

End: 635125

Strand: Direct

Name: mltD [H]

Synonym: Ping_0499

Alternate gene names: 119944273

Gene position: 633551-635125 (Clockwise)

Preceding gene: 119944272

Following gene: 119944276

Centisome position: 13.89

GC content: 41.14

Gene sequence:

>1575_bases
ATGAAATTTTTTCTGTCGGTTTTTATTCTGTTTTTTGTTACCGCCTGTACTAGTACAGGTGAGTTGGCTGGGCCCGAACC
CTCCAGAAAAAACATTGAAGAGCTACAACAAGAGATGTTCAGTCGGTCTGCCGAATGGAGAACGGCACCTTTTATGGACG
AAAAGTCCCTTCCCGAAAGTTATGATAATCTTTGGTTAAAGTTAGCTGAAGGTTTTGAATTTGAAGTGCCTCAAAACGCG
CGTGTCGCTAAACAACGGGATTATTTTTTAAAGCATCCAAATCACCTGGCGGAAGTTTCAAAACGAGCAGAACCTTTTTT
ATATCTGATTATTGAACAAATAGAAGCAAAAAACTTACCCCTTGAACTTGCTTTATTACCTGTTGTAGAAAGTACTTTTA
ATCCCTTTGCTTATTCGCAGAGTCATGCTTCCGGTTTGTGGCAATTTATGCCGGCCACAGGGGTACGATTCGGATTAACT
CAAGACTGGTGGTACGACGGTCGTCGCGACGTTTATGCTTCAACAGATGCAGCTTTAACCTATATGGATATATTACATAA
CTATTTAAATGAAGACTGGTTACATGCACTCGCGGCCTATAACTCTGGAGAAGGGCGGGTAGAAAGAGCGGTTCGCGCTA
ATAAGAAATACAATAAACCGACAGATTACTGGCATTTGTCCTTGCCAATAGAGACGCAAGAGTACGTCCCAAAGCTATTA
GCTTTAGTTGATATTTTACGTAACCACAAAAAGTATGGTATTGAGCTGCCAGTGATAGCAAATAAGCAGGTACTGACTTA
CGTAGATACCGGTTCGCAACTAGATCTTGCTTATGCGGCAGAATTGGCCGAGTTATCACCTGACGATCTTCAGTTATTAA
ATCCCGCTTATAATCGCTGGGTAACTTCACCAAACGGTCCTCACCGATTACTTATCCCGACAAAAATTGCACAGCAGTTT
AGTCAAAAATTGGCTAATACGGCGCAAAATAAGCGCACCAGATGGTCTCGTTATACTGTGCAGTCAGGTGATAACCTGGG
GTTCATTGCGCGCGAAAATTATACAACGATAAATATTCTTAAACAGGTTAACAACCTTGATAGTGCATTTATAAAAATAG
GACAGCCTTTACTTATTCCGTTTGCAAGTGAGGCCCAGAAGGATGATTTTTCTCGTCAGGCGAGGCGCTTTGAAGGTCAA
CAAGCCTCTGCAAACAAAACAAAAAAGAAAGTTATCTACACAGTAAGTGAAGCTGATACGCTCTGGGATATTAGTCGTGA
CTTTAATGTGAGGGTTAAAGAGATTGCCAAATGGAATACAATCAATAGTACAGAACCGTTACAGTTGGGGCAAAAATTAA
CCGTTTGGAAAGCGATTAAATCCACACCCAATAAAAACGATACTCACCGTATGACCTATCAGGTACGCGCCGGAGACTCT
CTTCACCTGATTGCATCAAAATTTAATGTAAAGCTCGTCGATTTAGTGCGTTGGAATGAGTTGAATAAAGGTGAATATAT
TCAGCCGGGGCAAAAATTAGAACTCTATGTTGCAGTGCAAAAATCTCGGACTTAA

Upstream 100 bases:

>100_bases
TCTCTTTGACACGTTCTTCCCACTCAGCGGCATTAATCAATTTGGTATTAGGACAAATTAAGCTAGAATACTCGGCTTAT
TCACAGTGGGTAGTATTTTT

Downstream 100 bases:

>100_bases
CTGGATGGATAATCTATAAAAAAAACCGCTAAGAAGCGGTTTTTTCATAGACAGTTAAAATAAATATTTACTGGGTAATC
TGTAATAATGCATTTTTGCC

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKFFLSVFILFFVTACTSTGELAGPEPSRKNIEELQQEMFSRSAEWRTAPFMDEKSLPESYDNLWLKLAEGFEFEVPQNA
RVAKQRDYFLKHPNHLAEVSKRAEPFLYLIIEQIEAKNLPLELALLPVVESTFNPFAYSQSHASGLWQFMPATGVRFGLT
QDWWYDGRRDVYASTDAALTYMDILHNYLNEDWLHALAAYNSGEGRVERAVRANKKYNKPTDYWHLSLPIETQEYVPKLL
ALVDILRNHKKYGIELPVIANKQVLTYVDTGSQLDLAYAAELAELSPDDLQLLNPAYNRWVTSPNGPHRLLIPTKIAQQF
SQKLANTAQNKRTRWSRYTVQSGDNLGFIARENYTTINILKQVNNLDSAFIKIGQPLLIPFASEAQKDDFSRQARRFEGQ
QASANKTKKKVIYTVSEADTLWDISRDFNVRVKEIAKWNTINSTEPLQLGQKLTVWKAIKSTPNKNDTHRMTYQVRAGDS
LHLIASKFNVKLVDLVRWNELNKGEYIQPGQKLELYVAVQKSRT

Sequences:

>Translated_524_residues
MKFFLSVFILFFVTACTSTGELAGPEPSRKNIEELQQEMFSRSAEWRTAPFMDEKSLPESYDNLWLKLAEGFEFEVPQNA
RVAKQRDYFLKHPNHLAEVSKRAEPFLYLIIEQIEAKNLPLELALLPVVESTFNPFAYSQSHASGLWQFMPATGVRFGLT
QDWWYDGRRDVYASTDAALTYMDILHNYLNEDWLHALAAYNSGEGRVERAVRANKKYNKPTDYWHLSLPIETQEYVPKLL
ALVDILRNHKKYGIELPVIANKQVLTYVDTGSQLDLAYAAELAELSPDDLQLLNPAYNRWVTSPNGPHRLLIPTKIAQQF
SQKLANTAQNKRTRWSRYTVQSGDNLGFIARENYTTINILKQVNNLDSAFIKIGQPLLIPFASEAQKDDFSRQARRFEGQ
QASANKTKKKVIYTVSEADTLWDISRDFNVRVKEIAKWNTINSTEPLQLGQKLTVWKAIKSTPNKNDTHRMTYQVRAGDS
LHLIASKFNVKLVDLVRWNELNKGEYIQPGQKLELYVAVQKSRT
>Mature_524_residues
MKFFLSVFILFFVTACTSTGELAGPEPSRKNIEELQQEMFSRSAEWRTAPFMDEKSLPESYDNLWLKLAEGFEFEVPQNA
RVAKQRDYFLKHPNHLAEVSKRAEPFLYLIIEQIEAKNLPLELALLPVVESTFNPFAYSQSHASGLWQFMPATGVRFGLT
QDWWYDGRRDVYASTDAALTYMDILHNYLNEDWLHALAAYNSGEGRVERAVRANKKYNKPTDYWHLSLPIETQEYVPKLL
ALVDILRNHKKYGIELPVIANKQVLTYVDTGSQLDLAYAAELAELSPDDLQLLNPAYNRWVTSPNGPHRLLIPTKIAQQF
SQKLANTAQNKRTRWSRYTVQSGDNLGFIARENYTTINILKQVNNLDSAFIKIGQPLLIPFASEAQKDDFSRQARRFEGQ
QASANKTKKKVIYTVSEADTLWDISRDFNVRVKEIAKWNTINSTEPLQLGQKLTVWKAIKSTPNKNDTHRMTYQVRAGDS
LHLIASKFNVKLVDLVRWNELNKGEYIQPGQKLELYVAVQKSRT

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=478, Percent_Identity=35.1464435146443, Blast_Score=292, Evalue=3e-80,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 60251; Mature: 60251

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFLSVFILFFVTACTSTGELAGPEPSRKNIEELQQEMFSRSAEWRTAPFMDEKSLPES
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
YDNLWLKLAEGFEFEVPQNARVAKQRDYFLKHPNHLAEVSKRAEPFLYLIIEQIEAKNLP
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEHHHHCCCCC
LELALLPVVESTFNPFAYSQSHASGLWQFMPATGVRFGLTQDWWYDGRRDVYASTDAALT
EEEEEHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEECCCHHHH
YMDILHNYLNEDWLHALAAYNSGEGRVERAVRANKKYNKPTDYWHLSLPIETQEYVPKLL
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHH
ALVDILRNHKKYGIELPVIANKQVLTYVDTGSQLDLAYAAELAELSPDDLQLLNPAYNRW
HHHHHHHCCHHCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCHHHHCC
VTSPNGPHRLLIPTKIAQQFSQKLANTAQNKRTRWSRYTVQSGDNLGFIARENYTTINIL
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHH
KQVNNLDSAFIKIGQPLLIPFASEAQKDDFSRQARRFEGQQASANKTKKKVIYTVSEADT
HHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCH
LWDISRDFNVRVKEIAKWNTINSTEPLQLGQKLTVWKAIKSTPNKNDTHRMTYQVRAGDS
HEECCCCCCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEECCCE
LHLIASKFNVKLVDLVRWNELNKGEYIQPGQKLELYVAVQKSRT
EEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MKFFLSVFILFFVTACTSTGELAGPEPSRKNIEELQQEMFSRSAEWRTAPFMDEKSLPES
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
YDNLWLKLAEGFEFEVPQNARVAKQRDYFLKHPNHLAEVSKRAEPFLYLIIEQIEAKNLP
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEHHHHCCCCC
LELALLPVVESTFNPFAYSQSHASGLWQFMPATGVRFGLTQDWWYDGRRDVYASTDAALT
EEEEEHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEECCCHHHH
YMDILHNYLNEDWLHALAAYNSGEGRVERAVRANKKYNKPTDYWHLSLPIETQEYVPKLL
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHH
ALVDILRNHKKYGIELPVIANKQVLTYVDTGSQLDLAYAAELAELSPDDLQLLNPAYNRW
HHHHHHHCCHHCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCHHHHCC
VTSPNGPHRLLIPTKIAQQFSQKLANTAQNKRTRWSRYTVQSGDNLGFIARENYTTINIL
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHH
KQVNNLDSAFIKIGQPLLIPFASEAQKDDFSRQARRFEGQQASANKTKKKVIYTVSEADT
HHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCH
LWDISRDFNVRVKEIAKWNTINSTEPLQLGQKLTVWKAIKSTPNKNDTHRMTYQVRAGDS
HEECCCCCCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEECCCE
LHLIASKFNVKLVDLVRWNELNKGEYIQPGQKLELYVAVQKSRT
EEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]