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The map label for this gene is gloB

Identifier: 119944272

GI number: 119944272

Start: 632621

End: 633391

Strand: Direct

Name: gloB

Synonym: Ping_0498

Alternate gene names: 119944272

Gene position: 632621-633391 (Clockwise)

Preceding gene: 119944270

Following gene: 119944273

Centisome position: 13.87

GC content: 36.58

Gene sequence:

>771_bases
ATGGAAAATGTAATAACAATTAAAGCATTTGAGGATAACTATATTTGGCTTATCAAAGATAGTCAAAGTCAACACTGTAT
CATTGTTGATCCCGGTGATGCCGCGCCCGTTTTAACAATATTAGAAGATCAGAAATTGATTGTAGATGCTATTCTTTTAA
CACACCATCATTATGATCATATTGGGGGTGTTGATGCATTATTATCTGCACGGGATGAAAAAATCTCAATCTATAGCAAA
AAGAAATTATTTGACCGCTGCAGGTTAGTAAATGAAAGTGATACTTTAAGCTTTTTTGACGGTAAACTTTCATTGCAGGT
GATGGAAGTGCCTGGGCATACCTTGGATCATGTTGCCTTTTATAATGACGAGCTGCTATTTTGCGGAGATACCCTATTTT
CAGGCGGGTGCGGGCGTGTATTTGAAGGTAGTTTCGAACAAATGTTTAAAGCGGTGTCTCGTCTGGCACTGTTACCGGAA
AATACAAAAGTATATTGTGCTCATGAATATACTCAAAATAATCTTATTTTTGCACATCAGATTGAACCTAAAAATAAGGC
ATTATTAAATTATATACAACAAGTCAGCAAAAAACGTCAGCAGGGGCAGCCAACAATCCCTTCAACAATAGGGTTAGAAA
AGGAAATTAATCCCTTCCTGCGCTGCCAGCAACAAACGGTCATTAATAAGTTACAAAGTCATTTAGGTAAAGAGTTAAAT
GATCCGCTTAGCTGCTTTAGTGCGTTGCGTCAGTATAAAGATAATTTTTAA

Upstream 100 bases:

>100_bases
GGTAATTGTTGCCAGGACCTGAGAGGGGGTAAAATACGGGATGTTTTGGCAGACCTCATGGGTTGAGTCACTCCAAGTTA
AATTAAATAGAGGTTATGTA

Downstream 100 bases:

>100_bases
TTATACGGATACCCAAATTACTTGAAGATGCAGATTTCTGCATTTTCAAGTAATTTGGGTCTCTTTGACACGTTCTTCCC
ACTCAGCGGCATTAATCAAT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MENVITIKAFEDNYIWLIKDSQSQHCIIVDPGDAAPVLTILEDQKLIVDAILLTHHHYDHIGGVDALLSARDEKISIYSK
KKLFDRCRLVNESDTLSFFDGKLSLQVMEVPGHTLDHVAFYNDELLFCGDTLFSGGCGRVFEGSFEQMFKAVSRLALLPE
NTKVYCAHEYTQNNLIFAHQIEPKNKALLNYIQQVSKKRQQGQPTIPSTIGLEKEINPFLRCQQQTVINKLQSHLGKELN
DPLSCFSALRQYKDNF

Sequences:

>Translated_256_residues
MENVITIKAFEDNYIWLIKDSQSQHCIIVDPGDAAPVLTILEDQKLIVDAILLTHHHYDHIGGVDALLSARDEKISIYSK
KKLFDRCRLVNESDTLSFFDGKLSLQVMEVPGHTLDHVAFYNDELLFCGDTLFSGGCGRVFEGSFEQMFKAVSRLALLPE
NTKVYCAHEYTQNNLIFAHQIEPKNKALLNYIQQVSKKRQQGQPTIPSTIGLEKEINPFLRCQQQTVINKLQSHLGKELN
DPLSCFSALRQYKDNF
>Mature_256_residues
MENVITIKAFEDNYIWLIKDSQSQHCIIVDPGDAAPVLTILEDQKLIVDAILLTHHHYDHIGGVDALLSARDEKISIYSK
KKLFDRCRLVNESDTLSFFDGKLSLQVMEVPGHTLDHVAFYNDELLFCGDTLFSGGCGRVFEGSFEQMFKAVSRLALLPE
NTKVYCAHEYTQNNLIFAHQIEPKNKALLNYIQQVSKKRQQGQPTIPSTIGLEKEINPFLRCQQQTVINKLQSHLGKELN
DPLSCFSALRQYKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=34.8484848484849, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=34.8484848484849, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI116642887, Length=263, Percent_Identity=34.2205323193916, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI21703352, Length=264, Percent_Identity=34.0909090909091, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI46361987, Length=208, Percent_Identity=31.25, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=40.625, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787158, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536925, Length=256, Percent_Identity=29.6875, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320478, Length=236, Percent_Identity=33.0508474576271, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324614, Length=242, Percent_Identity=30.1652892561983, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=36.6795366795367, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=36.6795366795367, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=36.6795366795367, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI20129357, Length=193, Percent_Identity=29.5336787564767, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_PSYIN (A1SS88)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941952.1
- ProteinModelPortal:   A1SS88
- SMR:   A1SS88
- STRING:   A1SS88
- GeneID:   4623404
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0498
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   A1SS88
- BioCyc:   PING357804:PING_0498-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 29147; Mature: 29147

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVITIKAFEDNYIWLIKDSQSQHCIIVDPGDAAPVLTILEDQKLIVDAILLTHHHYDH
CCCEEEEEEECCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCHH
IGGVDALLSARDEKISIYSKKKLFDRCRLVNESDTLSFFDGKLSLQVMEVPGHTLDHVAF
CCCHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCHHHEEE
YNDELLFCGDTLFSGGCGRVFEGSFEQMFKAVSRLALLPENTKVYCAHEYTQNNLIFAHQ
ECCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEE
IEPKNKALLNYIQQVSKKRQQGQPTIPSTIGLEKEINPFLRCQQQTVINKLQSHLGKELN
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHCC
DPLSCFSALRQYKDNF
CHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENVITIKAFEDNYIWLIKDSQSQHCIIVDPGDAAPVLTILEDQKLIVDAILLTHHHYDH
CCCEEEEEEECCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCHH
IGGVDALLSARDEKISIYSKKKLFDRCRLVNESDTLSFFDGKLSLQVMEVPGHTLDHVAF
CCCHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCHHHEEE
YNDELLFCGDTLFSGGCGRVFEGSFEQMFKAVSRLALLPENTKVYCAHEYTQNNLIFAHQ
ECCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEE
IEPKNKALLNYIQQVSKKRQQGQPTIPSTIGLEKEINPFLRCQQQTVINKLQSHLGKELN
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHCC
DPLSCFSALRQYKDNF
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA