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The map label for this gene is pilT [H]

Identifier: 119944268

GI number: 119944268

Start: 627570

End: 628697

Strand: Direct

Name: pilT [H]

Synonym: Ping_0494

Alternate gene names: 119944268

Gene position: 627570-628697 (Clockwise)

Preceding gene: 119944267

Following gene: 119944270

Centisome position: 13.76

GC content: 39.89

Gene sequence:

>1128_bases
ATGGAATTTGAAGAGATGCTCAATATTTTGTCGAGCAAAGATGGCTCGGATCTTTACCTGTCAACGGGGGCTCCCCCTTC
AGCTAAATTTCAAGGTACATTAGAACCTTTAACGGATATTCCTTTTCGGGCAGGTGAAGTTGCTGAAATAGCCGCTTCGA
TTATGGATAGTGAACAAAAAGTGGCATTTGAGCAAGAGCTTGAAATGAACTTAGCGATATCTATTTCCAAGGTTGGACGC
TTTCGAGTTAATATTTTTAAACAGCGTAATGAAGTTTCAATCGTTGCGCGTAATATTAAAATTGAGATCCCCAGCCTCGA
TGAATTAGGGCTTCCGGAAATACTTAAAAGTGTGATCACGGCTAAACGAGGTTTAGTGCTATTTATCGGTGCAACGGGCT
CAGGTAAATCGACTTCATTAGCAGCCCTTATTGATCACCGAAATACTAATCTCTCCGGTCATATTATTACCATCGAAGAT
CCTATTGAGTTTGTACACCAGCATAAAAAAAGTATCGTCAATCAGCGTGAAGTAGGAGTTGATACGCGTAGTTTTCATGC
GGCCTTAAAAAATACTCTGCGTCAGGCACCCGATGTTATTTTGATTGGTGAAATCCGCGATAGGGAAACAATGGAGCACG
CATTAGCCTTTGCAGAAACCGGCCACTTAGCTATTTCAACATTACATGCGAATAATGCTAACCAGGCATTGGATCGTATT
ATTAACTTCTTTCCAGAAGATCGTCGTATTCAGTTGTTAAGTGATTTAGGTAATAACTTGAAATCCTTTATTTCTCAACG
TTTAATTCCAACGATTGATGGCAAACGTTGTGCTGCCATTGAAGTCATGATGGGAAGCCTAACGATACGCGATATGATTA
AGCGCGGTGATTTTGGTTTGCTGAAAGAGGTGATGGAAAAATCAGAAGGATTAGGGATGAAAACCTTTGATGGTGCATTA
TTTGATCTCACCATTGCAGGAAAAATATCTGAAGAGGAAGCGATAAAAAATTCAGATTCAGCCACTAACCTAACGCTGAA
ATTTAAGTTGTACCATGGTAATACTACTGAAGAAGCAGGACAAGACGACAAAAAATCAGGTGGATTGACGTTATCAATGA
TGGATTAA

Upstream 100 bases:

>100_bases
GGCAGTAAAAAATAGAGACATTTAAAGTTGTTAATTGGCAGGGCTTCTTTATAATTTCATTATTATGGCTAACAAATGAT
TTTTAATTTAGGAGTTACAG

Downstream 100 bases:

>100_bases
TCCATTAACGTCTCTTGTTATTAGATAGTTATTAGATTGGCATGGAGAGAAATCCCAAATACTCATTGCTTTGTTTTTAT
ATTTTTAACTGTGATTTTAA

Product: type II secretion system protein (twitching motility protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQKVAFEQELEMNLAISISKVGR
FRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVITAKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIED
PIEFVHQHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGLLKEVMEKSEGLGMKTFDGAL
FDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAGQDDKKSGGLTLSMMD

Sequences:

>Translated_375_residues
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQKVAFEQELEMNLAISISKVGR
FRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVITAKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIED
PIEFVHQHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGLLKEVMEKSEGLGMKTFDGAL
FDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAGQDDKKSGGLTLSMMD
>Mature_375_residues
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQKVAFEQELEMNLAISISKVGR
FRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVITAKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIED
PIEFVHQHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGLLKEVMEKSEGLGMKTFDGAL
FDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAGQDDKKSGGLTLSMMD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=316, Percent_Identity=33.5443037974684, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1789723, Length=170, Percent_Identity=40.5882352941176, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786296, Length=225, Percent_Identity=31.1111111111111, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41281; Mature: 41281

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQK
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCHHH
VAFEQELEMNLAISISKVGRFRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVIT
HHHHHHCCEEEEEEEECCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHH
AKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIEDPIEFVHQHKKSIVNQREVGV
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHH
LKEVMEKSEGLGMKTFDGALFDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAG
HHHHHHHHCCCCCEECCCEEEEEEEECCCCHHHHHCCCCCCEEEEEEEEEEECCCCHHCC
QDDKKSGGLTLSMMD
CCCCCCCCEEEEECC
>Mature Secondary Structure
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQK
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCHHH
VAFEQELEMNLAISISKVGRFRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVIT
HHHHHHCCEEEEEEEECCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHH
AKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIEDPIEFVHQHKKSIVNQREVGV
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHH
LKEVMEKSEGLGMKTFDGALFDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAG
HHHHHHHHCCCCCEECCCEEEEEEEECCCCHHHHHCCCCCCEEEEEEEEEEECCCCHHCC
QDDKKSGGLTLSMMD
CCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]