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The map label for this gene is nagE [H]

Identifier: 119944266

GI number: 119944266

Start: 623506

End: 624963

Strand: Direct

Name: nagE [H]

Synonym: Ping_0491

Alternate gene names: 119944266

Gene position: 623506-624963 (Clockwise)

Preceding gene: 119944262

Following gene: 119944267

Centisome position: 13.67

GC content: 43.83

Gene sequence:

>1458_bases
ATGAATATTCTTGGTTATTTGCAAAAAGTAGGGCGCGCATTAATGGTGCCTGTTGCAGTGCTTCCTGCTGCGGCAATTTT
AATGGGTCTGGGTTACTGGATCGATCCAAATGGCTGGGGGGGAGATTCAGCGTTAGCTGCATTCTTGATTAAAGCAGGGG
CGGCGGTGATCGATAACATGTCTATGTTATTTGCCGTGGGTGTTGCTTACGGTCTGTCAAAAGATAAAGACGGTGCTGCA
GCGCTGTCAGGTTTAGTCGGTTTTTTGGTTGTCACTACACTGCTTTCTCCTGCTGCGGTTGCACAAATTCAAGGTATTGC
ACTTGCAGATGTCCCTTTTGCATTCAATAAAATTAACAATCAGTTTGTGGGTATCTTAGTCGGCATTTTCTCAGCTGAAC
TTTATAATAAATTCTCAAATGTTGAACTGCATTCGGCATTAGCTTTCTTTTCCGGCCGTCGTTTAGTGCCCATAGTCGTC
TCTGTGGTGATGATCTTTGTGTCATTCGTGCTGATGTTTATATGGCCCATTATCTTTTCCGGTCTACAAAATTTTGGTGA
AACCATTTCAGGAATGGGTGAAATTGGTGCCGGTATTTACGGATTCTTTAACCGTTTACTAATTCCTGTTGGTCTCCACC
ATGCACTTAACTCTGTATTCTGGTTTGATGTTGCAGGTATTAATGATATTCCCAACTTTTTAGGTGGCCAGTCATCATTA
GATGCAGGTACTGCCATTCTGGGTAAAACGGGGATGTATCAAGCTGGTTTCTTCCCTATTATGATGTTTGGCCTCCCCGG
CGCGGCATTGGCTATCTATAAAGCTGCTAAACCTGAAAATAAAGCAAAAACAGCTTCGCTTATGCTGGCGGCTGCTTTAG
CTTCTTTTTTTACGGGTGTCACAGAACCGCTGGAATTTTCCTTTATGTTCGCCGCACCCATTTTATATGTGATCCACGCA
TTATTAACTGGTTTTTCACTCTACATCGCGGCTTCAATGGAGTGGATGGCGGGCTTTGGTTTCTCAGCCGGTCTGGTGGA
TATGGTGCTTTCCTCACAAAATCCATTAGCTAAAAAATGGTATATGTTGTTGGTGCAGGGTGTTGCTTTCTTTGCTATCT
ACTACTTCGTTTTTCTGGCCTTAATTAAAGGCCTGAATCTTAAAACACCGGGCCGTGAAGATGACGAGGTGAATGCTTCA
ACCGCATCAGCATCTGAAGATTCAGGGGAATTAGCGGCGCAATATGTTATTGCATTGGGCGGACGTGATAACCTTGTGGT
TATTGACGCCTGCATTACGCGCTTACGTTTAACGCTTAAAGATCGCAGTCTGATTTGTGAGGCGGATCTTAAAGCATTAG
GTGCGATGGGAGTGGTTAAAATCGGCGATGATAACTTACAAGTTATTATTGGTCCGCAGGCAGAAAGTATCGCTGATCGT
ATCAAAGCGGTTAAATAA

Upstream 100 bases:

>100_bases
AGTATAAATAAACAGAAAATTAATGGATTTGGTATTAAACAATCAAACATAAAAAACCTAACACTAAAATGATTAGTTCA
TAAAATAAAGGAGAGTTAAT

Downstream 100 bases:

>100_bases
AGACTGGCTGTCACTGGGAGCTGTTTATTCTTCCGGAAGCTGTTTTTTCTCTCTGCAGGGCAGAGAGAGCGTTAATTCTA
TATAAGCTAAAATAAGCCCC

Product: PTS system, N-acetylglucosamine-specific IIBC subunit

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNILGYLQKVGRALMVPVAVLPAAAILMGLGYWIDPNGWGGDSALAAFLIKAGAAVIDNMSMLFAVGVAYGLSKDKDGAA
ALSGLVGFLVVTTLLSPAAVAQIQGIALADVPFAFNKINNQFVGILVGIFSAELYNKFSNVELHSALAFFSGRRLVPIVV
SVVMIFVSFVLMFIWPIIFSGLQNFGETISGMGEIGAGIYGFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQSSL
DAGTAILGKTGMYQAGFFPIMMFGLPGAALAIYKAAKPENKAKTASLMLAAALASFFTGVTEPLEFSFMFAAPILYVIHA
LLTGFSLYIAASMEWMAGFGFSAGLVDMVLSSQNPLAKKWYMLLVQGVAFFAIYYFVFLALIKGLNLKTPGREDDEVNAS
TASASEDSGELAAQYVIALGGRDNLVVIDACITRLRLTLKDRSLICEADLKALGAMGVVKIGDDNLQVIIGPQAESIADR
IKAVK

Sequences:

>Translated_485_residues
MNILGYLQKVGRALMVPVAVLPAAAILMGLGYWIDPNGWGGDSALAAFLIKAGAAVIDNMSMLFAVGVAYGLSKDKDGAA
ALSGLVGFLVVTTLLSPAAVAQIQGIALADVPFAFNKINNQFVGILVGIFSAELYNKFSNVELHSALAFFSGRRLVPIVV
SVVMIFVSFVLMFIWPIIFSGLQNFGETISGMGEIGAGIYGFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQSSL
DAGTAILGKTGMYQAGFFPIMMFGLPGAALAIYKAAKPENKAKTASLMLAAALASFFTGVTEPLEFSFMFAAPILYVIHA
LLTGFSLYIAASMEWMAGFGFSAGLVDMVLSSQNPLAKKWYMLLVQGVAFFAIYYFVFLALIKGLNLKTPGREDDEVNAS
TASASEDSGELAAQYVIALGGRDNLVVIDACITRLRLTLKDRSLICEADLKALGAMGVVKIGDDNLQVIIGPQAESIADR
IKAVK
>Mature_485_residues
MNILGYLQKVGRALMVPVAVLPAAAILMGLGYWIDPNGWGGDSALAAFLIKAGAAVIDNMSMLFAVGVAYGLSKDKDGAA
ALSGLVGFLVVTTLLSPAAVAQIQGIALADVPFAFNKINNQFVGILVGIFSAELYNKFSNVELHSALAFFSGRRLVPIVV
SVVMIFVSFVLMFIWPIIFSGLQNFGETISGMGEIGAGIYGFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQSSL
DAGTAILGKTGMYQAGFFPIMMFGLPGAALAIYKAAKPENKAKTASLMLAAALASFFTGVTEPLEFSFMFAAPILYVIHA
LLTGFSLYIAASMEWMAGFGFSAGLVDMVLSSQNPLAKKWYMLLVQGVAFFAIYYFVFLALIKGLNLKTPGREDDEVNAS
TASASEDSGELAAQYVIALGGRDNLVVIDACITRLRLTLKDRSLICEADLKALGAMGVVKIGDDNLQVIIGPQAESIADR
IKAVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=491, Percent_Identity=44.6028513238289, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1787343, Length=501, Percent_Identity=38.12375249501, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787908, Length=518, Percent_Identity=33.7837837837838, Blast_Score=272, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51471; Mature: 51471

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGYLQKVGRALMVPVAVLPAAAILMGLGYWIDPNGWGGDSALAAFLIKAGAAVIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
SMLFAVGVAYGLSKDKDGAAALSGLVGFLVVTTLLSPAAVAQIQGIALADVPFAFNKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEECCCHHHHHHCH
QFVGILVGIFSAELYNKFSNVELHSALAFFSGRRLVPIVVSVVMIFVSFVLMFIWPIIFS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLQNFGETISGMGEIGAGIYGFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQSSL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCC
DAGTAILGKTGMYQAGFFPIMMFGLPGAALAIYKAAKPENKAKTASLMLAAALASFFTGV
CCCHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFAAPILYVIHALLTGFSLYIAASMEWMAGFGFSAGLVDMVLSSQNPLAKKW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
YMLLVQGVAFFAIYYFVFLALIKGLNLKTPGREDDEVNASTASASEDSGELAAQYVIALG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEC
GRDNLVVIDACITRLRLTLKDRSLICEADLKALGAMGVVKIGDDNLQVIIGPQAESIADR
CCCCEEEHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHH
IKAVK
HHCCC
>Mature Secondary Structure
MNILGYLQKVGRALMVPVAVLPAAAILMGLGYWIDPNGWGGDSALAAFLIKAGAAVIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
SMLFAVGVAYGLSKDKDGAAALSGLVGFLVVTTLLSPAAVAQIQGIALADVPFAFNKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEECCCHHHHHHCH
QFVGILVGIFSAELYNKFSNVELHSALAFFSGRRLVPIVVSVVMIFVSFVLMFIWPIIFS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLQNFGETISGMGEIGAGIYGFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQSSL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCC
DAGTAILGKTGMYQAGFFPIMMFGLPGAALAIYKAAKPENKAKTASLMLAAALASFFTGV
CCCHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFAAPILYVIHALLTGFSLYIAASMEWMAGFGFSAGLVDMVLSSQNPLAKKW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
YMLLVQGVAFFAIYYFVFLALIKGLNLKTPGREDDEVNASTASASEDSGELAAQYVIALG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEC
GRDNLVVIDACITRLRLTLKDRSLICEADLKALGAMGVVKIGDDNLQVIIGPQAESIADR
CCCCEEEHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHH
IKAVK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]