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The map label for this gene is crp [H]

Identifier: 119944168

GI number: 119944168

Start: 494356

End: 494994

Strand: Reverse

Name: crp [H]

Synonym: Ping_0388

Alternate gene names: 119944168

Gene position: 494994-494356 (Counterclockwise)

Preceding gene: 119944169

Following gene: 119944159

Centisome position: 10.86

GC content: 38.5

Gene sequence:

>639_bases
ATGATAATAACCAAACCTCAAACCGATCCAACGTTGGAGTGGTTTTTATCACACTGTCACACACATAAATATCCAGCTAA
AAGTATACTTATTCATGCTGGAGAAAAGGCTGATACACTTTATTTCATCGTTAAAGGCTCGGTTGCAGTTGTGATTAAGG
ATGAAGAAGGTAAAGAAATGATTTTATCTTACTTAAATCAAGGTGATTTTATTGGTGAATTAGGTTTATTTGATAAACAA
GAAAATCCAATTCGCAGTGCTTCAATTAAGGCAAAAACTCCCTGTGAAGTTGCTGAAATTTCTTATAAGAAGTTCAATCA
ACTGATCACTGTCAATCCTGAAATTTTAATGCGTTTATCAAAGCAAATGGCTGATCGTTTACAGATTACAAGCCAAAAAG
TGGGTAACCTTGCATTTTTGGATGTCACAGGCCGTATCGCAAATACACTGCTGGATCTTGCCAAGCAGCCGGACGCAATG
ACCCACCCCGATGGCATGCAAATCAAAATCACTCGTCAAGAAATAGGTCAGATTGTCGGTTGTTCACGTGAAACTGTTGG
TCGAATCCTTAAAATGCTTGAGGAACAAGGTCTTATTTCTGCTCACGGTAAAACAATTGTGGTATTCGGGACGCGCTAA

Upstream 100 bases:

>100_bases
TAAAATTCTATTCCATGTAAGTAACTGTCATTATTATTTTGGCGAATAAACCAAAACAAGAAATAGCACATTAGCTATTC
ATACCTATTAAGGGAGATTT

Downstream 100 bases:

>100_bases
GCCAACCGATAATATGCAAAAAACCGCTTAATTGCGGTTTTTTTAACAGTAAAATTTAGGTATAAACGGATAATTACGAG
GGTAACCCCGCCTGCAGGAA

Product: cAMP-regulatory protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIITKPQTDPTLEWFLSHCHTHKYPAKSILIHAGEKADTLYFIVKGSVAVVIKDEEGKEMILSYLNQGDFIGELGLFDKQ
ENPIRSASIKAKTPCEVAEISYKKFNQLITVNPEILMRLSKQMADRLQITSQKVGNLAFLDVTGRIANTLLDLAKQPDAM
THPDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQGLISAHGKTIVVFGTR

Sequences:

>Translated_212_residues
MIITKPQTDPTLEWFLSHCHTHKYPAKSILIHAGEKADTLYFIVKGSVAVVIKDEEGKEMILSYLNQGDFIGELGLFDKQ
ENPIRSASIKAKTPCEVAEISYKKFNQLITVNPEILMRLSKQMADRLQITSQKVGNLAFLDVTGRIANTLLDLAKQPDAM
THPDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQGLISAHGKTIVVFGTR
>Mature_212_residues
MIITKPQTDPTLEWFLSHCHTHKYPAKSILIHAGEKADTLYFIVKGSVAVVIKDEEGKEMILSYLNQGDFIGELGLFDKQ
ENPIRSASIKAKTPCEVAEISYKKFNQLITVNPEILMRLSKQMADRLQITSQKVGNLAFLDVTGRIANTLLDLAKQPDAM
THPDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQGLISAHGKTIVVFGTR

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=212, Percent_Identity=84.9056603773585, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 23649; Mature: 23649

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00888 CNMP_BINDING_1 ; PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITKPQTDPTLEWFLSHCHTHKYPAKSILIHAGEKADTLYFIVKGSVAVVIKDEEGKEM
CEECCCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCEEEEEEECCEEEEEECCCCHHH
ILSYLNQGDFIGELGLFDKQENPIRSASIKAKTPCEVAEISYKKFNQLITVNPEILMRLS
HHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECHHHHHHHH
KQMADRLQITSQKVGNLAFLDVTGRIANTLLDLAKQPDAMTHPDGMQIKITRQEIGQIVG
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
CSRETVGRILKMLEEQGLISAHGKTIVVFGTR
CCHHHHHHHHHHHHHCCCEEECCCEEEEEECC
>Mature Secondary Structure
MIITKPQTDPTLEWFLSHCHTHKYPAKSILIHAGEKADTLYFIVKGSVAVVIKDEEGKEM
CEECCCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCEEEEEEECCEEEEEECCCCHHH
ILSYLNQGDFIGELGLFDKQENPIRSASIKAKTPCEVAEISYKKFNQLITVNPEILMRLS
HHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECHHHHHHHH
KQMADRLQITSQKVGNLAFLDVTGRIANTLLDLAKQPDAMTHPDGMQIKITRQEIGQIVG
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
CSRETVGRILKMLEEQGLISAHGKTIVVFGTR
CCHHHHHHHHHHHHHCCCEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]