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The map label for this gene is ubiE

Identifier: 119944108

GI number: 119944108

Start: 422433

End: 423188

Strand: Reverse

Name: ubiE

Synonym: Ping_0323

Alternate gene names: 119944108

Gene position: 423188-422433 (Counterclockwise)

Preceding gene: 119944110

Following gene: 119944107

Centisome position: 9.28

GC content: 41.93

Gene sequence:

>756_bases
ATGAGCAACCAAGCAGAAAACACAACACATTTTGGCTTTAAAACCGTGCAGGAAGATACAAAAGCAGATTTAGTTGCTGG
CGTTTTTCATTCGGTAGCCGCTAAATACGATATTATGAATGATGTTTTATCAATGGGAGTTCATCGCATTTGGAAACGCT
TTACTGTTGATTGTTCAGGCATACGAAAAGGTCAAAAAGTATTAGATCTGGCTGGTGGAACGGGTGATTTAACGGCTAAA
TTCTCCCGTATAGTCGGTCAAACGGGACAAGTCACTCTCGCTGATATTAACGACTCGATGCTCAAAGTGGGTCGCAGCAA
GCTTCGCGATCTGGGGATTGTCGGCAATGTCAGTTATGTACAAGCGAATGCCGAAGAGTTACCTTTTCCTGACAACCATT
TTGATTTAATTACTATTGCCTTTGGGTTACGTAATGTCACCGATAAGGACAAAGCGTTGGCCTCTATGTATCGCGTTTTA
AAGCCGGGCGGTCGTCTGTTAGTGCTTGAATTCTCGACACCTCTGTACGAACCGCTCAGTAAATTTTACGATTTTTACTC
GTTTAACGTTCTTCCTAAATTGGGCAAGTTGATTGCAAACGACAGTGAAAGTTACCAGTATCTTGCAGAAAGTATTCGCA
TGCATCCCGGTCAAGAGATCTTAAAAGAGATGATGAATAGCGCTAATTTTGAAGGCTGCGAATATTTTAATCTCAGTGGT
GGTATTGTGGCACTGCACCGTGGTTACAAATATTAA

Upstream 100 bases:

>100_bases
ATCCAATTTTCGACAAAATCAGGTATTATCTTCGATCTTATTTTAGATCTACCCTAAGCCCGTTAGGGATAGAATCTGTC
ACAGCATAAAGGGAAAAGAC

Downstream 100 bases:

>100_bases
CCAATATGCACTTCTAGAGCTTCGGTACTAGGCTATCCCAGCTCCTACTTGCTAACATCAACGCTAAAATAGAGGATATT
ATGCCATTAGATAAGCTAGT

Product: ubiquinone/menaquinone biosynthesis methyltransferases

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSNQAENTTHFGFKTVQEDTKADLVAGVFHSVAAKYDIMNDVLSMGVHRIWKRFTVDCSGIRKGQKVLDLAGGTGDLTAK
FSRIVGQTGQVTLADINDSMLKVGRSKLRDLGIVGNVSYVQANAEELPFPDNHFDLITIAFGLRNVTDKDKALASMYRVL
KPGGRLLVLEFSTPLYEPLSKFYDFYSFNVLPKLGKLIANDSESYQYLAESIRMHPGQEILKEMMNSANFEGCEYFNLSG
GIVALHRGYKY

Sequences:

>Translated_251_residues
MSNQAENTTHFGFKTVQEDTKADLVAGVFHSVAAKYDIMNDVLSMGVHRIWKRFTVDCSGIRKGQKVLDLAGGTGDLTAK
FSRIVGQTGQVTLADINDSMLKVGRSKLRDLGIVGNVSYVQANAEELPFPDNHFDLITIAFGLRNVTDKDKALASMYRVL
KPGGRLLVLEFSTPLYEPLSKFYDFYSFNVLPKLGKLIANDSESYQYLAESIRMHPGQEILKEMMNSANFEGCEYFNLSG
GIVALHRGYKY
>Mature_250_residues
SNQAENTTHFGFKTVQEDTKADLVAGVFHSVAAKYDIMNDVLSMGVHRIWKRFTVDCSGIRKGQKVLDLAGGTGDLTAKF
SRIVGQTGQVTLADINDSMLKVGRSKLRDLGIVGNVSYVQANAEELPFPDNHFDLITIAFGLRNVTDKDKALASMYRVLK
PGGRLLVLEFSTPLYEPLSKFYDFYSFNVLPKLGKLIANDSESYQYLAESIRMHPGQEILKEMMNSANFEGCEYFNLSGG
IVALHRGYKY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=275, Percent_Identity=44.7272727272727, Blast_Score=229, Evalue=1e-60,
Organism=Escherichia coli, GI48994983, Length=251, Percent_Identity=72.5099601593625, Blast_Score=406, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17557039, Length=245, Percent_Identity=45.7142857142857, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=41.2451361867704, Blast_Score=172, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24641702, Length=258, Percent_Identity=45.7364341085271, Blast_Score=239, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_PSYIN (A1SRS4)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941788.1
- ProteinModelPortal:   A1SRS4
- SMR:   A1SRS4
- STRING:   A1SRS4
- GeneID:   4624763
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0323
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   A1SRS4
- BioCyc:   PING357804:PING_0323-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27955; Mature: 27824

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQAENTTHFGFKTVQEDTKADLVAGVFHSVAAKYDIMNDVLSMGVHRIWKRFTVDCSG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IRKGQKVLDLAGGTGDLTAKFSRIVGQTGQVTLADINDSMLKVGRSKLRDLGIVGNVSYV
HHCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCEE
QANAEELPFPDNHFDLITIAFGLRNVTDKDKALASMYRVLKPGGRLLVLEFSTPLYEPLS
ECCHHHCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KFYDFYSFNVLPKLGKLIANDSESYQYLAESIRMHPGQEILKEMMNSANFEGCEYFNLSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCC
GIVALHRGYKY
CEEEECCCCCC
>Mature Secondary Structure 
SNQAENTTHFGFKTVQEDTKADLVAGVFHSVAAKYDIMNDVLSMGVHRIWKRFTVDCSG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IRKGQKVLDLAGGTGDLTAKFSRIVGQTGQVTLADINDSMLKVGRSKLRDLGIVGNVSYV
HHCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCEE
QANAEELPFPDNHFDLITIAFGLRNVTDKDKALASMYRVLKPGGRLLVLEFSTPLYEPLS
ECCHHHCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KFYDFYSFNVLPKLGKLIANDSESYQYLAESIRMHPGQEILKEMMNSANFEGCEYFNLSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCC
GIVALHRGYKY
CEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA