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The map label for this gene is ykoW [H]

Identifier: 119944007

GI number: 119944007

Start: 276412

End: 278772

Strand: Reverse

Name: ykoW [H]

Synonym: Ping_0221

Alternate gene names: 119944007

Gene position: 278772-276412 (Counterclockwise)

Preceding gene: 119944009

Following gene: 119944006

Centisome position: 6.11

GC content: 42.65

Gene sequence:

>2361_bases
ATGGATCGAATTTTAAAATCTCGCAAGATAGATCAAACCAGCCTGGGTCGCTGCATGTTAATTTTTATGACGGGGTTATT
AGTCAGTATTGGAACATTATTTTGGTGGGGAGCATATAGCCAGCTCGCCACAAGCTTCGATGCTTTTGACGTTAGCTATT
ATGAAAACGAATTAAAAAGAGTTGCCGGAGTGCTAAAACAGAATCAACTCTCATTCGAACTGCAAATAAAAGACTATGCC
CATTGGGATGATACAGAAGCATTCGTATCAGGCGACTATCCTGTTTATATAGAAGAAAACTTTACCCCAATATCGCTCGC
GAACAATAAGATCAGCGGCTATCTTATTGCCAGACTGGATGGCTCCTTTGCGGCTCCCCCCATGATACTTTCCGATGATG
CTCTTATTCCCATGCCCGCAAAGATGAGATCGGTATTGTCTCCTTTGTTACCAGCTTTAATGACTGCCGATAAACCGACA
GCCAACACGGCGCTATTTTGGCTTAATGATCAAGCCACTTTTGTTTCTGGCGTGACCATCACAGATTCTAATGAATCAAG
ACCCCCAAGCGGTTATCTGTTGTTTTTTCGTCATTTTGATAGCGCTATATTAGATGGATTCAGATCTTTAACCTCGGTCG
ATTTTACTTTACTGCCGCAGCTTGAAAACAATAAAGCAAACTTTAAGGTGCAGTATTTATCAGACTTGGACGAGCCAGCC
TGGGTAGTCAGCAAAAACCTGAAAAATTTACCCGCTATGATTGAAATACAGGGGACAACACATTTAAAAAAAGAACGTTA
CATGAGCTTTGTATTATTGGGGAGCAGTGTTGCGGGCTTGACACTCTTTTCATTGCTGGGTATTTATCTATGCCTTCATT
TTAGGATTCTAAAAAGGCTGCATATTTTTTCTCAGCTTGCGGATAAGCATCGATTGTCACCACAACAGCCTGTACGTTGG
CCGATTAAAAATAACGATGAACTTGATAATCTTGCTTTTGCTTTGAATGAGTTAATGTCTGAAGTGGAAGTGAGGCATAA
AGAGATGAGTTTTCTTGCAGACCATGATCCCCTTACGGGATTAGGTAACCGACGCTTATTGATGGCACAACTCGATGCCA
ATATAAACCGGCAGAAACGCCATCCAACTTTTATCAGTACTCTTTTACTTATTGATCTCGATGAATTCAAATTACTCAAT
GATGGACTGGGACACAATGCGGGAGACGATATTCTTAAACTTATGTCCCTGCGCATGCTCTCACAAGTACGCAATTACGA
TACGGTTGTTCGCTTAGGCGGTGATGAGTTCGCTATCCTGCTCGATGGCGTTGGACTGGAGATGGCACAATCTTTTGCCG
AGCGACTGCTGGACACAATCACTCAGCCCTTCAAACAAAACGGTCAAGACTTGATCTTAAGGGCTTCGATCGGGCTAACG
GCAGTCAATAATGGCCTCTGCAAAGAAAATCTGATCAGATATGCTGATTTAGCGATGTACGAAGCTAAACGACGTGGTAA
GGGACAAGTGACCATTTTTAAAATGGCTCTGCTCGATACGGTTTCCCGCCGGGTGCTGTTAGAACAGGCACTCCATTCCG
CACTTAATGATGATCAGCTTGAAGTGTGGTTTCAACCGATAGTTGATTTGAACAGCGGGGAGGTGGTAAATATGGAGGCG
CTATCACGCTGGTCACTTGCCGGAGAATTTGTGCCACCGGAAGAGTTTATTAATATTGCTGAAATAACAGGAATGATTAT
ACCACTGGGGCAACAATTATTCGACAAGGTGGGCTGCGCTCTGCAGGAGTTACGGGTAGAACATCCGAATCTTCAATGCA
ATATTAACCTATCGGTCAAACAATTCAGGGATAGGAATTTATTTACCGAGATCACCTCCTGCCTAAATAAATATCAGTTG
CCCACGTCGGCTTTCCATTTAGAGCTGACCGAAAGTATGGTAGTGGAGACGGAAACAGATATTTTACCTATGATGCAAAA
ACTGGTTGAGCATGGTTTAAAATTTCACTTAGATGACTTTGGCACAGGATATTCTTCGCTCGATCGTCTCAATAATCTGC
CTTTTGATACGCTAAAAATCGATCGCAGCTTTGTGACTTCGCTAGGCATGGGTGATGATATTATGGCTAGAAATATTATC
AACATAGGCAACGAATTGGGTCTGAATATTATTGCAGAAGGCGTCGAGACCCAAGTCGAGCTGGATAGATTAATGGATTT
AGGCTGCTCCCAGATTCAAGGTTATTATTTTGCTAAACCTATGCCCTTAAGCGAGCTCAAAGACTGGCTGTATGACAAAC
AGCATCAGGTGATACAAATGAAATCAAAACGACAAGCCTAG

Upstream 100 bases:

>100_bases
ATAAAATCACAAAAATATAGTTAACTGGCTTATTTCTTCTGTCACATTGAGCATATTGTTCAATTTTGAATATCAGCGAT
TGAACCTAAGGACTAAATCC

Downstream 100 bases:

>100_bases
GGCTGTTATATCTTAACTTTACACCTACTTAAAGGATAAATAAGGGTGAATAACTGCTAGGAAAACTAAGTAAAATAAGA
GGTTAAGCAATCAAATCCAG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MDRILKSRKIDQTSLGRCMLIFMTGLLVSIGTLFWWGAYSQLATSFDAFDVSYYENELKRVAGVLKQNQLSFELQIKDYA
HWDDTEAFVSGDYPVYIEENFTPISLANNKISGYLIARLDGSFAAPPMILSDDALIPMPAKMRSVLSPLLPALMTADKPT
ANTALFWLNDQATFVSGVTITDSNESRPPSGYLLFFRHFDSAILDGFRSLTSVDFTLLPQLENNKANFKVQYLSDLDEPA
WVVSKNLKNLPAMIEIQGTTHLKKERYMSFVLLGSSVAGLTLFSLLGIYLCLHFRILKRLHIFSQLADKHRLSPQQPVRW
PIKNNDELDNLAFALNELMSEVEVRHKEMSFLADHDPLTGLGNRRLLMAQLDANINRQKRHPTFISTLLLIDLDEFKLLN
DGLGHNAGDDILKLMSLRMLSQVRNYDTVVRLGGDEFAILLDGVGLEMAQSFAERLLDTITQPFKQNGQDLILRASIGLT
AVNNGLCKENLIRYADLAMYEAKRRGKGQVTIFKMALLDTVSRRVLLEQALHSALNDDQLEVWFQPIVDLNSGEVVNMEA
LSRWSLAGEFVPPEEFINIAEITGMIIPLGQQLFDKVGCALQELRVEHPNLQCNINLSVKQFRDRNLFTEITSCLNKYQL
PTSAFHLELTESMVVETETDILPMMQKLVEHGLKFHLDDFGTGYSSLDRLNNLPFDTLKIDRSFVTSLGMGDDIMARNII
NIGNELGLNIIAEGVETQVELDRLMDLGCSQIQGYYFAKPMPLSELKDWLYDKQHQVIQMKSKRQA

Sequences:

>Translated_786_residues
MDRILKSRKIDQTSLGRCMLIFMTGLLVSIGTLFWWGAYSQLATSFDAFDVSYYENELKRVAGVLKQNQLSFELQIKDYA
HWDDTEAFVSGDYPVYIEENFTPISLANNKISGYLIARLDGSFAAPPMILSDDALIPMPAKMRSVLSPLLPALMTADKPT
ANTALFWLNDQATFVSGVTITDSNESRPPSGYLLFFRHFDSAILDGFRSLTSVDFTLLPQLENNKANFKVQYLSDLDEPA
WVVSKNLKNLPAMIEIQGTTHLKKERYMSFVLLGSSVAGLTLFSLLGIYLCLHFRILKRLHIFSQLADKHRLSPQQPVRW
PIKNNDELDNLAFALNELMSEVEVRHKEMSFLADHDPLTGLGNRRLLMAQLDANINRQKRHPTFISTLLLIDLDEFKLLN
DGLGHNAGDDILKLMSLRMLSQVRNYDTVVRLGGDEFAILLDGVGLEMAQSFAERLLDTITQPFKQNGQDLILRASIGLT
AVNNGLCKENLIRYADLAMYEAKRRGKGQVTIFKMALLDTVSRRVLLEQALHSALNDDQLEVWFQPIVDLNSGEVVNMEA
LSRWSLAGEFVPPEEFINIAEITGMIIPLGQQLFDKVGCALQELRVEHPNLQCNINLSVKQFRDRNLFTEITSCLNKYQL
PTSAFHLELTESMVVETETDILPMMQKLVEHGLKFHLDDFGTGYSSLDRLNNLPFDTLKIDRSFVTSLGMGDDIMARNII
NIGNELGLNIIAEGVETQVELDRLMDLGCSQIQGYYFAKPMPLSELKDWLYDKQHQVIQMKSKRQA
>Mature_786_residues
MDRILKSRKIDQTSLGRCMLIFMTGLLVSIGTLFWWGAYSQLATSFDAFDVSYYENELKRVAGVLKQNQLSFELQIKDYA
HWDDTEAFVSGDYPVYIEENFTPISLANNKISGYLIARLDGSFAAPPMILSDDALIPMPAKMRSVLSPLLPALMTADKPT
ANTALFWLNDQATFVSGVTITDSNESRPPSGYLLFFRHFDSAILDGFRSLTSVDFTLLPQLENNKANFKVQYLSDLDEPA
WVVSKNLKNLPAMIEIQGTTHLKKERYMSFVLLGSSVAGLTLFSLLGIYLCLHFRILKRLHIFSQLADKHRLSPQQPVRW
PIKNNDELDNLAFALNELMSEVEVRHKEMSFLADHDPLTGLGNRRLLMAQLDANINRQKRHPTFISTLLLIDLDEFKLLN
DGLGHNAGDDILKLMSLRMLSQVRNYDTVVRLGGDEFAILLDGVGLEMAQSFAERLLDTITQPFKQNGQDLILRASIGLT
AVNNGLCKENLIRYADLAMYEAKRRGKGQVTIFKMALLDTVSRRVLLEQALHSALNDDQLEVWFQPIVDLNSGEVVNMEA
LSRWSLAGEFVPPEEFINIAEITGMIIPLGQQLFDKVGCALQELRVEHPNLQCNINLSVKQFRDRNLFTEITSCLNKYQL
PTSAFHLELTESMVVETETDILPMMQKLVEHGLKFHLDDFGTGYSSLDRLNNLPFDTLKIDRSFVTSLGMGDDIMARNII
NIGNELGLNIIAEGVETQVELDRLMDLGCSQIQGYYFAKPMPLSELKDWLYDKQHQVIQMKSKRQA

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=435, Percent_Identity=33.7931034482759, Blast_Score=245, Evalue=9e-66,
Organism=Escherichia coli, GI87081921, Length=435, Percent_Identity=30.1149425287356, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI226510982, Length=448, Percent_Identity=27.9017857142857, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=33.739837398374, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI87081743, Length=244, Percent_Identity=33.6065573770492, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786507, Length=258, Percent_Identity=30.6201550387597, Blast_Score=130, Evalue=5e-31,
Organism=Escherichia coli, GI1788502, Length=257, Percent_Identity=31.9066147859922, Blast_Score=125, Evalue=2e-29,
Organism=Escherichia coli, GI1787055, Length=298, Percent_Identity=28.5234899328859, Blast_Score=123, Evalue=6e-29,
Organism=Escherichia coli, GI87081845, Length=251, Percent_Identity=31.0756972111554, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788381, Length=434, Percent_Identity=26.4976958525346, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI87081980, Length=263, Percent_Identity=30.0380228136882, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI145693134, Length=185, Percent_Identity=40, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI87082096, Length=423, Percent_Identity=22.9314420803783, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788849, Length=263, Percent_Identity=27.7566539923954, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=29.940119760479, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=30.9090909090909, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788956, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=32.9341317365269, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=190, Percent_Identity=29.4736842105263, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=33.7579617834395, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI87081974, Length=101, Percent_Identity=33.6633663366337, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1789650, Length=465, Percent_Identity=22.3655913978495, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 88917; Mature: 88917

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRILKSRKIDQTSLGRCMLIFMTGLLVSIGTLFWWGAYSQLATSFDAFDVSYYENELKR
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAGVLKQNQLSFELQIKDYAHWDDTEAFVSGDYPVYIEENFTPISLANNKISGYLIARLD
HHHHHHCCCCEEEEEEECCCCCCCCCCEECCCCEEEEECCCCEEEECCCCCCEEEEEEEC
GSFAAPPMILSDDALIPMPAKMRSVLSPLLPALMTADKPTANTALFWLNDQATFVSGVTI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEE
TDSNESRPPSGYLLFFRHFDSAILDGFRSLTSVDFTLLPQLENNKANFKVQYLSDLDEPA
ECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCCCCCCH
WVVSKNLKNLPAMIEIQGTTHLKKERYMSFVLLGSSVAGLTLFSLLGIYLCLHFRILKRL
HHHHHHHCCCCEEEEECCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
HIFSQLADKHRLSPQQPVRWPIKNNDELDNLAFALNELMSEVEVRHKEMSFLADHDPLTG
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LGNRRLLMAQLDANINRQKRHPTFISTLLLIDLDEFKLLNDGLGHNAGDDILKLMSLRML
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
SQVRNYDTVVRLGGDEFAILLDGVGLEMAQSFAERLLDTITQPFKQNGQDLILRASIGLT
HHHHCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AVNNGLCKENLIRYADLAMYEAKRRGKGQVTIFKMALLDTVSRRVLLEQALHSALNDDQL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVWFQPIVDLNSGEVVNMEALSRWSLAGEFVPPEEFINIAEITGMIIPLGQQLFDKVGCA
HHHHHHHEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LQELRVEHPNLQCNINLSVKQFRDRNLFTEITSCLNKYQLPTSAFHLELTESMVVETETD
HHHHHCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEEEHHHHHEECCHH
ILPMMQKLVEHGLKFHLDDFGTGYSSLDRLNNLPFDTLKIDRSFVTSLGMGDDIMARNII
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHEEHHHHHHHHCCCCCHHHHHHHH
NIGNELGLNIIAEGVETQVELDRLMDLGCSQIQGYYFAKPMPLSELKDWLYDKQHQVIQM
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCHHHHHHHHHCCHHHHHHH
KSKRQA
HHHCCC
>Mature Secondary Structure
MDRILKSRKIDQTSLGRCMLIFMTGLLVSIGTLFWWGAYSQLATSFDAFDVSYYENELKR
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAGVLKQNQLSFELQIKDYAHWDDTEAFVSGDYPVYIEENFTPISLANNKISGYLIARLD
HHHHHHCCCCEEEEEEECCCCCCCCCCEECCCCEEEEECCCCEEEECCCCCCEEEEEEEC
GSFAAPPMILSDDALIPMPAKMRSVLSPLLPALMTADKPTANTALFWLNDQATFVSGVTI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEE
TDSNESRPPSGYLLFFRHFDSAILDGFRSLTSVDFTLLPQLENNKANFKVQYLSDLDEPA
ECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCCCCCCH
WVVSKNLKNLPAMIEIQGTTHLKKERYMSFVLLGSSVAGLTLFSLLGIYLCLHFRILKRL
HHHHHHHCCCCEEEEECCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
HIFSQLADKHRLSPQQPVRWPIKNNDELDNLAFALNELMSEVEVRHKEMSFLADHDPLTG
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LGNRRLLMAQLDANINRQKRHPTFISTLLLIDLDEFKLLNDGLGHNAGDDILKLMSLRML
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
SQVRNYDTVVRLGGDEFAILLDGVGLEMAQSFAERLLDTITQPFKQNGQDLILRASIGLT
HHHHCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AVNNGLCKENLIRYADLAMYEAKRRGKGQVTIFKMALLDTVSRRVLLEQALHSALNDDQL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVWFQPIVDLNSGEVVNMEALSRWSLAGEFVPPEEFINIAEITGMIIPLGQQLFDKVGCA
HHHHHHHEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LQELRVEHPNLQCNINLSVKQFRDRNLFTEITSCLNKYQLPTSAFHLELTESMVVETETD
HHHHHCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEEEHHHHHEECCHH
ILPMMQKLVEHGLKFHLDDFGTGYSSLDRLNNLPFDTLKIDRSFVTSLGMGDDIMARNII
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHEEHHHHHHHHCCCCCHHHHHHHH
NIGNELGLNIIAEGVETQVELDRLMDLGCSQIQGYYFAKPMPLSELKDWLYDKQHQVIQM
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCHHHHHHHHHCCHHHHHHH
KSKRQA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]