| Definition | Azoarcus sp. BH72 chromosome, complete genome. |
|---|---|
| Accession | NC_008702 |
| Length | 4,376,040 |
Click here to switch to the map view.
The map label for this gene is yafB [H]
Identifier: 119899920
GI number: 119899920
Start: 3982119
End: 3982925
Strand: Direct
Name: yafB [H]
Synonym: azo3631
Alternate gene names: 119899920
Gene position: 3982119-3982925 (Clockwise)
Preceding gene: 119899919
Following gene: 119899921
Centisome position: 91.0
GC content: 68.77
Gene sequence:
>807_bases ATGTCCATCCCCATGCCCGCCATCGGCCTTGGCACCTTCCGCCTGCAGGGCCAGACCGTGATCGACTCGGTCCGCAGCGG CCTTGAGCTCGGCTACCGCCACATCGACACCGCGCAGATCTACGCCAACGAGGCCGAAGTCGGCCTGGCGATCGCGGAGA GCAGCGTCGCCCGCGACCAGCTCTTCATCACCACCAAGATCTGGACGGAGAACCTCGCCGCCGAAAAGCTGATCCCCAGC CTGGAAGACAGCCTCGCCAGGCTGCGCGTGGAGCACGTCGACCTGACGCTGATCCACTGGCCTTCGCCCGGCGAAGCGGT GCCGCTCGCCGAAAGCCTGATGGCGCTGCAGGAAGCCAAGCGCCGCGGGCTGACCCGGCAGATCGGCGTCTCCAACTTCA CCATCGCCCACCTGCAGCAGGCCATCGCGCTGCTCGGCGCCGACGAGATCGCCACCAACCAGGTGGAACTCCACCCGCTG CTGCAGAACCGCAAGCTCGCCGGATTCGCCCGCGCACAAGGCATCCACCTCACCGCCTACATGCCGCTCGCCTACGGCAA GGTGCTGGCGGAGCCGGTGGTGCAGCGTATCGCCGCTGCGCACGACGCCACCCCCGCGCAGGTCGCACTCGCGTGGTTGC TGCAGCAGGACCATGCGGTGATTCCGTCCTCCACCCGGCGCGAGAACCTCGCCAGCAACCTCGCCGCCGCCCGCTTGCAC CTGAGCGCTGCGGAGCGCGCCGAAATCGCCGCATGCGACCGTGGCGAGCGGATGGCCAATCCGGACTTTGCGCCGCGCTG GGACTGA
Upstream 100 bases:
>100_bases TGGAGCGGCCGGCTGGACCGCCGCGACCCCGACTACGACGAACTCGCCCCGGCCCCCGCTGCCGCGGCCGCCCCCTGATC ACCACAAACAGGAGCACCCC
Downstream 100 bases:
>100_bases CACGCCCGCACACCCCGGCGGACATGGCGGCAAATGAAGGACAATCCGCCCCATGTCCTCCGACCTCACGCCACTCGCCG CCTCGCCCCGCCGCCTGTCC
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 268; Mature: 267
Protein sequence:
>268_residues MSIPMPAIGLGTFRLQGQTVIDSVRSGLELGYRHIDTAQIYANEAEVGLAIAESSVARDQLFITTKIWTENLAAEKLIPS LEDSLARLRVEHVDLTLIHWPSPGEAVPLAESLMALQEAKRRGLTRQIGVSNFTIAHLQQAIALLGADEIATNQVELHPL LQNRKLAGFARAQGIHLTAYMPLAYGKVLAEPVVQRIAAAHDATPAQVALAWLLQQDHAVIPSSTRRENLASNLAAARLH LSAAERAEIAACDRGERMANPDFAPRWD
Sequences:
>Translated_268_residues MSIPMPAIGLGTFRLQGQTVIDSVRSGLELGYRHIDTAQIYANEAEVGLAIAESSVARDQLFITTKIWTENLAAEKLIPS LEDSLARLRVEHVDLTLIHWPSPGEAVPLAESLMALQEAKRRGLTRQIGVSNFTIAHLQQAIALLGADEIATNQVELHPL LQNRKLAGFARAQGIHLTAYMPLAYGKVLAEPVVQRIAAAHDATPAQVALAWLLQQDHAVIPSSTRRENLASNLAAARLH LSAAERAEIAACDRGERMANPDFAPRWD >Mature_267_residues SIPMPAIGLGTFRLQGQTVIDSVRSGLELGYRHIDTAQIYANEAEVGLAIAESSVARDQLFITTKIWTENLAAEKLIPSL EDSLARLRVEHVDLTLIHWPSPGEAVPLAESLMALQEAKRRGLTRQIGVSNFTIAHLQQAIALLGADEIATNQVELHPLL QNRKLAGFARAQGIHLTAYMPLAYGKVLAEPVVQRIAAAHDATPAQVALAWLLQQDHAVIPSSTRRENLASNLAAARLHL SAAERAEIAACDRGERMANPDFAPRWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=32.312925170068, Blast_Score=150, Evalue=1e-36, Organism=Homo sapiens, GI223468663, Length=288, Percent_Identity=32.6388888888889, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=32.312925170068, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=32.8519855595668, Blast_Score=149, Evalue=2e-36, Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=32.312925170068, Blast_Score=149, Evalue=3e-36, Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=32.312925170068, Blast_Score=149, Evalue=3e-36, Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=30.5084745762712, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=31.4878892733564, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=31.4878892733564, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=31.9727891156463, Blast_Score=145, Evalue=3e-35, Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=34.2560553633218, Blast_Score=142, Evalue=3e-34, Organism=Homo sapiens, GI93277124, Length=300, Percent_Identity=29.6666666666667, Blast_Score=139, Evalue=2e-33, Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=30.8219178082192, Blast_Score=132, Evalue=5e-31, Organism=Homo sapiens, GI291291012, Length=286, Percent_Identity=31.1188811188811, Blast_Score=130, Evalue=9e-31, Organism=Homo sapiens, GI300116273, Length=271, Percent_Identity=32.1033210332103, Blast_Score=128, Evalue=5e-30, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=29.8561151079137, Blast_Score=108, Evalue=4e-24, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=40.3846153846154, Blast_Score=88, Evalue=1e-17, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=40.3846153846154, Blast_Score=88, Evalue=1e-17, Organism=Homo sapiens, GI310109924, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=1e-12, Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=60, Blast_Score=329, Evalue=1e-91, Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=37.9310344827586, Blast_Score=180, Evalue=9e-47, Organism=Escherichia coli, GI1788081, Length=263, Percent_Identity=33.4600760456274, Blast_Score=120, Evalue=1e-28, Organism=Escherichia coli, GI1787674, Length=266, Percent_Identity=28.9473684210526, Blast_Score=78, Evalue=6e-16, Organism=Escherichia coli, GI1788070, Length=305, Percent_Identity=25.9016393442623, Blast_Score=77, Evalue=9e-16, Organism=Escherichia coli, GI87081735, Length=295, Percent_Identity=25.0847457627119, Blast_Score=65, Evalue=3e-12, Organism=Escherichia coli, GI1789199, Length=329, Percent_Identity=25.8358662613982, Blast_Score=63, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17537075, Length=296, Percent_Identity=34.4594594594595, Blast_Score=152, Evalue=1e-37, Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=33.7931034482759, Blast_Score=149, Evalue=2e-36, Organism=Caenorhabditis elegans, GI17552492, Length=257, Percent_Identity=33.4630350194552, Blast_Score=141, Evalue=3e-34, Organism=Caenorhabditis elegans, GI17561300, Length=248, Percent_Identity=32.6612903225806, Blast_Score=135, Evalue=2e-32, Organism=Caenorhabditis elegans, GI71998625, Length=255, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=3e-32, Organism=Caenorhabditis elegans, GI17561298, Length=267, Percent_Identity=29.5880149812734, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=31.9587628865979, Blast_Score=134, Evalue=7e-32, Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=29.4339622641509, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=29.9319727891156, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=29.4314381270903, Blast_Score=120, Evalue=6e-28, Organism=Caenorhabditis elegans, GI17566692, Length=285, Percent_Identity=29.8245614035088, Blast_Score=118, Evalue=4e-27, Organism=Caenorhabditis elegans, GI17562292, Length=282, Percent_Identity=29.0780141843972, Blast_Score=114, Evalue=7e-26, Organism=Saccharomyces cerevisiae, GI6324694, Length=290, Percent_Identity=33.448275862069, Blast_Score=164, Evalue=1e-41, Organism=Saccharomyces cerevisiae, GI6320576, Length=264, Percent_Identity=35.2272727272727, Blast_Score=152, Evalue=4e-38, Organism=Saccharomyces cerevisiae, GI6322556, Length=249, Percent_Identity=31.7269076305221, Blast_Score=139, Evalue=4e-34, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=28.4313725490196, Blast_Score=122, Evalue=6e-29, Organism=Saccharomyces cerevisiae, GI6319625, Length=272, Percent_Identity=32.7205882352941, Blast_Score=110, Evalue=2e-25, Organism=Saccharomyces cerevisiae, GI6320079, Length=248, Percent_Identity=25.4032258064516, Blast_Score=81, Evalue=2e-16, Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=33.2225913621262, Blast_Score=146, Evalue=1e-35, Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=31.4641744548287, Blast_Score=138, Evalue=3e-33, Organism=Drosophila melanogaster, GI24657054, Length=286, Percent_Identity=31.8181818181818, Blast_Score=137, Evalue=9e-33, Organism=Drosophila melanogaster, GI24662789, Length=263, Percent_Identity=30.7984790874525, Blast_Score=130, Evalue=6e-31, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=30.1754385964912, Blast_Score=130, Evalue=7e-31, Organism=Drosophila melanogaster, GI24662785, Length=270, Percent_Identity=32.2222222222222, Blast_Score=127, Evalue=7e-30, Organism=Drosophila melanogaster, GI21356425, Length=282, Percent_Identity=29.4326241134752, Blast_Score=126, Evalue=1e-29, Organism=Drosophila melanogaster, GI20129731, Length=278, Percent_Identity=31.6546762589928, Blast_Score=126, Evalue=2e-29, Organism=Drosophila melanogaster, GI24662781, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI45553081, Length=291, Percent_Identity=29.2096219931272, Blast_Score=117, Evalue=1e-26, Organism=Drosophila melanogaster, GI281366140, Length=266, Percent_Identity=28.1954887218045, Blast_Score=99, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29173; Mature: 29042
Theoretical pI: Translated: 6.42; Mature: 6.42
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSIPMPAIGLGTFRLQGQTVIDSVRSGLELGYRHIDTAQIYANEAEVGLAIAESSVARDQ CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHEEECCCHHEEEEHHHHHCCCE LFITTKIWTENLAAEKLIPSLEDSLARLRVEHVDLTLIHWPSPGEAVPLAESLMALQEAK EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHH RRGLTRQIGVSNFTIAHLQQAIALLGADEIATNQVELHPLLQNRKLAGFARAQGIHLTAY HCCCEECCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEHHHHCCCHHHHHHCCCEEEEEE MPLAYGKVLAEPVVQRIAAAHDATPAQVALAWLLQQDHAVIPSSTRRENLASNLAAARLH CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH LSAAERAEIAACDRGERMANPDFAPRWD HHHHHHHHHHHCCCCCCCCCCCCCCCCC >Mature Secondary Structure SIPMPAIGLGTFRLQGQTVIDSVRSGLELGYRHIDTAQIYANEAEVGLAIAESSVARDQ CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHEEECCCHHEEEEHHHHHCCCE LFITTKIWTENLAAEKLIPSLEDSLARLRVEHVDLTLIHWPSPGEAVPLAESLMALQEAK EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHH RRGLTRQIGVSNFTIAHLQQAIALLGADEIATNQVELHPLLQNRKLAGFARAQGIHLTAY HCCCEECCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEHHHHCCCHHHHHHCCCEEEEEE MPLAYGKVLAEPVVQRIAAAHDATPAQVALAWLLQQDHAVIPSSTRRENLASNLAAARLH CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH LSAAERAEIAACDRGERMANPDFAPRWD HHHHHHHHHHHCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]