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The map label for this gene is cafA [H]

Identifier: 119899901

GI number: 119899901

Start: 3963871

End: 3965382

Strand: Direct

Name: cafA [H]

Synonym: azo3612

Alternate gene names: 119899901

Gene position: 3963871-3965382 (Clockwise)

Preceding gene: 119899900

Following gene: 119899902

Centisome position: 90.58

GC content: 64.68

Gene sequence:

>1512_bases
ATGCGCAATGACAGCACCACGGCTGTCATTGCGCATTTCCCATTACCGCCTCGAACCATGAGCATCGAGTTCCTCATCAA
CTTCACCCCGCAGGAGACGCGGGTCGCCATCGTCGAACAGGGCGTCGTGCAGGAACTGCACGTCGAACGCACGGCCAGCC
GGGGCATCGTCGGCAACATCTACCTCGGCAAGGTCGTACGGGTGCTGCCCGGCATGCAGTCCGCCTTCATCGAGATCGGC
CTCGAGCGCACCGCCTTCCTGCATGTGGCGGACATCTGGAGCGACCGCCAGAACGGCGAAGCGGCAAAGCCGATCGAGCG
CATCCTCGCCGAAGGCCAGAGCCTGGTCGTCCAGGTGCTCAAGGACCCGATCGGCACCAAGGGCGCCCGGCTCTCCACCC
AGATCAGCATCGCCGGCCGGCTGCTCGTCTACCTGCCCCAGGAAAAACACATCGGCATCTCGCAGCGGATCGAGAACGAG
ACCGAGCGCGAGGCGCTCCGCGAGCGCCTGACGCGCCTCGTGCCCGCCGACGAACCCGGCGGCTTCATCGTGCGCACCAT
GGCCGAGTCCGCCTCGGACGACGAACTCGCCGCCGACATCGCCTATCTGCGCAAGCTCTGGAACGAGATCCGCAACAACA
GCACCGGCGCCTTCCCGCCCAAGCTGCTGCATGAAGACCTCGGCCTGGGCCAGCGCGTGCTGCGCGACCTGGTCAACGAG
GAAACCACGCGCATCCGCGTCGATTCGCGGGAGAACTTCCAGAAGCTCGCCGCCTTCGCGGCCGAATACAGCCCCAAGGT
GCTGCCCTTGCTCGAGCACTACACCGGCGAGCGGCCGCTGTTCGACATCTTCAACATCGAAGAGGAAATCCAGAAGGCGC
TCGCGCGCCGGGTGGATCTCAAGTCGGGGGGCTACCTGATCATCGACCAGACCGAGGCGATGACCACGGTCGACGTCAAC
ACCGGCGGCTTCGTCGGCGCACGCAACTTCGACGACACCATCTTCAAGACCAATCTCGAGGCCACCCAGGCGATCGCCCG
CCAGCTACGCCTGCGCAACCTCGGCGGCATCATCATCATCGACTTCATCGACATGGAAAACGTCGAGCATCGCGAGATGG
TGCTGGAGGAATTCCGCAAAGCGCTCGCGCGCGACCACACCAAGATGAGCATCAACGGCTTCACCGCGCTCGGGCTGGTG
GAGATGACGCGCAAGCGCACCCGCGAGTCGCTGGCCCATCTGCTGTGCGAAGCCTGCCCCACCTGTGGCGGGCGGGGCGA
GGTCAAGACCGCCCGCACCGTGGCTTACGAGGTCCTGCGCGAACTGCTGCGCGAAGCCCGCCAGTTCAATGCACGCGAAT
TCCGCGTGCTCGCGGCGCCCAACGTCATCGACCTGTTCCTCGACGAGGAAAGCCAGTCGCTGGCGATGCTGTCGGACTTC
ATCGACAAGAAGATCTCGCTGCACCCCGAGGCGAGCTACACCCAGGAACAGTTCGACATCGTGCTGCTGTAA

Upstream 100 bases:

>100_bases
TTCGAAACCGCCCAGCTGCTGGAAGCCTTCGGCTATCCTCTCTAGCGGCGCCGCCGCCCGCGGGCGGCGGACGGGTAACG
ACGAGGCGTGGCGATGAGCC

Downstream 100 bases:

>100_bases
GGCGTCTTGTGCGTACTCCCGCCGAACCCCGCGCCGACAGCGGCCAGCCGCGCGCGCTCGTCCTCCTCGCCGCTACCCGC
CCGGCCTTTCTGTCGATCAC

Product: putative ribonuclease

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRNDSTTAVIAHFPLPPRTMSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNIYLGKVVRVLPGMQSAFIEIG
LERTAFLHVADIWSDRQNGEAAKPIERILAEGQSLVVQVLKDPIGTKGARLSTQISIAGRLLVYLPQEKHIGISQRIENE
TEREALRERLTRLVPADEPGGFIVRTMAESASDDELAADIAYLRKLWNEIRNNSTGAFPPKLLHEDLGLGQRVLRDLVNE
ETTRIRVDSRENFQKLAAFAAEYSPKVLPLLEHYTGERPLFDIFNIEEEIQKALARRVDLKSGGYLIIDQTEAMTTVDVN
TGGFVGARNFDDTIFKTNLEATQAIARQLRLRNLGGIIIIDFIDMENVEHREMVLEEFRKALARDHTKMSINGFTALGLV
EMTRKRTRESLAHLLCEACPTCGGRGEVKTARTVAYEVLRELLREARQFNAREFRVLAAPNVIDLFLDEESQSLAMLSDF
IDKKISLHPEASYTQEQFDIVLL

Sequences:

>Translated_503_residues
MRNDSTTAVIAHFPLPPRTMSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNIYLGKVVRVLPGMQSAFIEIG
LERTAFLHVADIWSDRQNGEAAKPIERILAEGQSLVVQVLKDPIGTKGARLSTQISIAGRLLVYLPQEKHIGISQRIENE
TEREALRERLTRLVPADEPGGFIVRTMAESASDDELAADIAYLRKLWNEIRNNSTGAFPPKLLHEDLGLGQRVLRDLVNE
ETTRIRVDSRENFQKLAAFAAEYSPKVLPLLEHYTGERPLFDIFNIEEEIQKALARRVDLKSGGYLIIDQTEAMTTVDVN
TGGFVGARNFDDTIFKTNLEATQAIARQLRLRNLGGIIIIDFIDMENVEHREMVLEEFRKALARDHTKMSINGFTALGLV
EMTRKRTRESLAHLLCEACPTCGGRGEVKTARTVAYEVLRELLREARQFNAREFRVLAAPNVIDLFLDEESQSLAMLSDF
IDKKISLHPEASYTQEQFDIVLL
>Mature_503_residues
MRNDSTTAVIAHFPLPPRTMSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNIYLGKVVRVLPGMQSAFIEIG
LERTAFLHVADIWSDRQNGEAAKPIERILAEGQSLVVQVLKDPIGTKGARLSTQISIAGRLLVYLPQEKHIGISQRIENE
TEREALRERLTRLVPADEPGGFIVRTMAESASDDELAADIAYLRKLWNEIRNNSTGAFPPKLLHEDLGLGQRVLRDLVNE
ETTRIRVDSRENFQKLAAFAAEYSPKVLPLLEHYTGERPLFDIFNIEEEIQKALARRVDLKSGGYLIIDQTEAMTTVDVN
TGGFVGARNFDDTIFKTNLEATQAIARQLRLRNLGGIIIIDFIDMENVEHREMVLEEFRKALARDHTKMSINGFTALGLV
EMTRKRTRESLAHLLCEACPTCGGRGEVKTARTVAYEVLRELLREARQFNAREFRVLAAPNVIDLFLDEESQSLAMLSDF
IDKKISLHPEASYTQEQFDIVLL

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=491, Percent_Identity=54.786150712831, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1787325, Length=463, Percent_Identity=35.4211663066955, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 56686; Mature: 56686

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNDSTTAVIAHFPLPPRTMSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNI
CCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
YLGKVVRVLPGMQSAFIEIGLERTAFLHVADIWSDRQNGEAAKPIERILAEGQSLVVQVL
HHHHHHHHHCCHHHHHHHHCCCHHHHEEHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
KDPIGTKGARLSTQISIAGRLLVYLPQEKHIGISQRIENETEREALRERLTRLVPADEPG
HCCCCCCCCCEEEEEEEECEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCC
GFIVRTMAESASDDELAADIAYLRKLWNEIRNNSTGAFPPKLLHEDLGLGQRVLRDLVNE
CEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHCC
ETTRIRVDSRENFQKLAAFAAEYSPKVLPLLEHYTGERPLFDIFNIEEEIQKALARRVDL
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHCCC
KSGGYLIIDQTEAMTTVDVNTGGFVGARNFDDTIFKTNLEATQAIARQLRLRNLGGIIII
CCCCEEEEECCCCEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEE
DFIDMENVEHREMVLEEFRKALARDHTKMSINGFTALGLVEMTRKRTRESLAHLLCEACP
EEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TCGGRGEVKTARTVAYEVLRELLREARQFNAREFRVLAAPNVIDLFLDEESQSLAMLSDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCHHHHHHHHH
IDKKISLHPEASYTQEQFDIVLL
HHCCEECCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MRNDSTTAVIAHFPLPPRTMSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNI
CCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
YLGKVVRVLPGMQSAFIEIGLERTAFLHVADIWSDRQNGEAAKPIERILAEGQSLVVQVL
HHHHHHHHHCCHHHHHHHHCCCHHHHEEHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
KDPIGTKGARLSTQISIAGRLLVYLPQEKHIGISQRIENETEREALRERLTRLVPADEPG
HCCCCCCCCCEEEEEEEECEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCC
GFIVRTMAESASDDELAADIAYLRKLWNEIRNNSTGAFPPKLLHEDLGLGQRVLRDLVNE
CEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHCC
ETTRIRVDSRENFQKLAAFAAEYSPKVLPLLEHYTGERPLFDIFNIEEEIQKALARRVDL
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHCCC
KSGGYLIIDQTEAMTTVDVNTGGFVGARNFDDTIFKTNLEATQAIARQLRLRNLGGIIII
CCCCEEEEECCCCEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEE
DFIDMENVEHREMVLEEFRKALARDHTKMSINGFTALGLVEMTRKRTRESLAHLLCEACP
EEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TCGGRGEVKTARTVAYEVLRELLREARQFNAREFRVLAAPNVIDLFLDEESQSLAMLSDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCHHHHHHHHH
IDKKISLHPEASYTQEQFDIVLL
HHCCEECCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]