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The map label for this gene is queF

Identifier: 119899798

GI number: 119899798

Start: 3850094

End: 3850939

Strand: Reverse

Name: queF

Synonym: azo3509

Alternate gene names: 119899798

Gene position: 3850939-3850094 (Counterclockwise)

Preceding gene: 119899799

Following gene: 119899797

Centisome position: 88.0

GC content: 69.15

Gene sequence:

>846_bases
ATGAACGATACGCAGAACCCGCTCCACGGCGCCGAAGCCTCCCCGCTCGGCAAGCCCGTGGCCTACCGCGACACCTACGC
GCCCGAGCTGCTGTTCCCGATCGCCCGCCAGCTCAAGCGCGACGAGATCGGCGTGCGCGCCGACGCCTTGCCCTTCTGCG
GCGAAGATCTGTGGAACGCCTACGAACTCTCCTGGCTCAATCCGCGCGGCAAGCCGGTGGTGGCGCTGGCGGAGTTCCGC
GTGCCCGCCGATACCCCCCGGCTGGTGGAATCCAAGTCGCTCAAGCTCTACCTCAACAGCTTCAACCAGAGCCGTTTCGA
TGACGCCGCCGCGGTGCGCATGGCGATCGCGCGCGATCTTTCCGCGGCTGCGGGCGGCGCGGTGGGGGTCGCCGTGTGGC
CGCTCGGGGGTCAGGCTGAACGCCGGTTCGCCGCGCCCGAGGGTGTCTGCATCGACGATCTGGACGTCGCGATCGACACC
TACCAGCCCGACCCGACGCTGCTTTCCGCCGGCGACGAGGTGGTCAGCGAAACGCTGTACTCGCATCTGCTCAAGACCAA
CTGCCTCGTCACCGGCCAGCCGGACTGGGGCATGGTGGCGGTGCGCTACACCGGGCCGCGCATCGATCGCGCCGGCCTGC
TGCGCTACATCGTCTCCTTCCGCGAGCACAACGAATTCCACGAACAGTGCGTCGAGCGGGTGTTCTGCGACATCACCGCG
CGCTGCCGTCCGCAGCGGCTGGCGGTGTGGGCGCGCTACACCCGGCGCGGCGGGCTGGACATCAACCCTTTCCGCGCCAG
CGACCGCGACCAGCGCGCGGACGAGGCGATGGAGATCCGCCAGTAA

Upstream 100 bases:

>100_bases
CGGCTCGCGCGCGGGCTGCACGAAACCGCGCGGCTGATGCTCTACAGCAACAAGGTCGATGCCGATTGAGCGCCCCGCGC
CGCGGCCCCCAATGCCTGCC

Downstream 100 bases:

>100_bases
GCGGGCTGCCCCGCCGCGGGCAACGCCCGGGTACGCATGCCCCATCCTGGTGCGATGGGTGGGCGATCGACCCGCCGACG
GGCCAAGGTGCCCCGGCGAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNDTQNPLHGAEASPLGKPVAYRDTYAPELLFPIARQLKRDEIGVRADALPFCGEDLWNAYELSWLNPRGKPVVALAEFR
VPADTPRLVESKSLKLYLNSFNQSRFDDAAAVRMAIARDLSAAAGGAVGVAVWPLGGQAERRFAAPEGVCIDDLDVAIDT
YQPDPTLLSAGDEVVSETLYSHLLKTNCLVTGQPDWGMVAVRYTGPRIDRAGLLRYIVSFREHNEFHEQCVERVFCDITA
RCRPQRLAVWARYTRRGGLDINPFRASDRDQRADEAMEIRQ

Sequences:

>Translated_281_residues
MNDTQNPLHGAEASPLGKPVAYRDTYAPELLFPIARQLKRDEIGVRADALPFCGEDLWNAYELSWLNPRGKPVVALAEFR
VPADTPRLVESKSLKLYLNSFNQSRFDDAAAVRMAIARDLSAAAGGAVGVAVWPLGGQAERRFAAPEGVCIDDLDVAIDT
YQPDPTLLSAGDEVVSETLYSHLLKTNCLVTGQPDWGMVAVRYTGPRIDRAGLLRYIVSFREHNEFHEQCVERVFCDITA
RCRPQRLAVWARYTRRGGLDINPFRASDRDQRADEAMEIRQ
>Mature_281_residues
MNDTQNPLHGAEASPLGKPVAYRDTYAPELLFPIARQLKRDEIGVRADALPFCGEDLWNAYELSWLNPRGKPVVALAEFR
VPADTPRLVESKSLKLYLNSFNQSRFDDAAAVRMAIARDLSAAAGGAVGVAVWPLGGQAERRFAAPEGVCIDDLDVAIDT
YQPDPTLLSAGDEVVSETLYSHLLKTNCLVTGQPDWGMVAVRYTGPRIDRAGLLRYIVSFREHNEFHEQCVERVFCDITA
RCRPQRLAVWARYTRRGGLDINPFRASDRDQRADEAMEIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=255, Percent_Identity=52.9411764705882, Blast_Score=285, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_AZOSB (A1KBB9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935011.1
- ProteinModelPortal:   A1KBB9
- SMR:   A1KBB9
- STRING:   A1KBB9
- GeneID:   4610073
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3509
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   A1KBB9
- ProtClustDB:   PRK11792
- BioCyc:   ASP62928:AZO3509-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31377; Mature: 31377

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTQNPLHGAEASPLGKPVAYRDTYAPELLFPIARQLKRDEIGVRADALPFCGEDLWNA
CCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCC
YELSWLNPRGKPVVALAEFRVPADTPRLVESKSLKLYLNSFNQSRFDDAAAVRMAIARDL
EEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SAAAGGAVGVAVWPLGGQAERRFAAPEGVCIDDLDVAIDTYQPDPTLLSAGDEVVSETLY
HHHCCCCEEEEEECCCCCHHHHCCCCCCCEECCCCEEEECCCCCCCHHHCCHHHHHHHHH
SHLLKTNCLVTGQPDWGMVAVRYTGPRIDRAGLLRYIVSFREHNEFHEQCVERVFCDITA
HHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHC
RCRPQRLAVWARYTRRGGLDINPFRASDRDQRADEAMEIRQ
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNDTQNPLHGAEASPLGKPVAYRDTYAPELLFPIARQLKRDEIGVRADALPFCGEDLWNA
CCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCC
YELSWLNPRGKPVVALAEFRVPADTPRLVESKSLKLYLNSFNQSRFDDAAAVRMAIARDL
EEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SAAAGGAVGVAVWPLGGQAERRFAAPEGVCIDDLDVAIDTYQPDPTLLSAGDEVVSETLY
HHHCCCCEEEEEECCCCCHHHHCCCCCCCEECCCCEEEECCCCCCCHHHCCHHHHHHHHH
SHLLKTNCLVTGQPDWGMVAVRYTGPRIDRAGLLRYIVSFREHNEFHEQCVERVFCDITA
HHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHC
RCRPQRLAVWARYTRRGGLDINPFRASDRDQRADEAMEIRQ
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA