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The map label for this gene is phrB [H]

Identifier: 119899747

GI number: 119899747

Start: 3796292

End: 3797803

Strand: Reverse

Name: phrB [H]

Synonym: azo3458

Alternate gene names: 119899747

Gene position: 3797803-3796292 (Counterclockwise)

Preceding gene: 119899753

Following gene: 119899740

Centisome position: 86.79

GC content: 68.65

Gene sequence:

>1512_bases
GTGTCTGCGGCGTGCGTTCTGCCGCTGGGCGATAATCCGCTCCCGTCCCTACCGATCACGCAGTGCCTCATGACCGTTGC
CCTTGTCTGGTTTCGCCGCGACCTCCGCTGCGTCGACCACGCCGCGCTGTATCACGCCCTGCGCGAGCATGAGCGCGTCT
ATTGCGCGTTCGTGTTCGACACGGACATTCTCGATGCGCTGCCGACGCGGGCCGACCGGCGGGTCGAGTTCATTCACGCC
GCGGTGGTCGAGCTTGACCGCGCGCTCGAGGCCCTGTCGCGCGAAGCCGGCGGCAGTGGTAGCGGGTTGATCGTGCGCCA
CGGCCGCGCGCAGGAGGCGATCCCGGCGCTTGCGCAGGCGCTCGGCGTGGATGCGGTATACGTGAACCGCGATTACGAGC
CGGCCGCCATCGCCCGCGACCGCGCCGTGGCGGAGCGCCTGGCCGAGCACGGCCGCACGCTGCGGGACTTCAAGGACCAG
GTCATCTTCGACCGAGACGAGGTGCTGACCCAGGCGGGCCAGCCCTTCAGTGTGTTCACGCCGTACAAGAACGCCTGGCT
GCGCAAGCTCGATCCGTTCTACCTGCAGGCCTATCCGATTGCGCCCCGTGCGCGCCGGCTGGCGCCGAAGCCCGCAGGGG
AGGGGGTGCCGGCGCTGGGCAGGATCGGCTTCGAACCCACGGATCTTCGCGGGCTGGCGCTGCCCACCGGGGCAAGCGGT
GCGCGCGCGCTGTTCGGGGATTTCTGCGGCCGCATCGAAGACTACCGCATCGCGCGCGACTTCCCGGCGGTGAAAGGCGT
GTCCTACCTGTCGACGCACCTGCGCTTCGGCACGATCCCGGTGCGTGAACTGGCGGCCTACGCGGTCCACCAGGGCGGTG
CCGGCGCGCAGACCTGGCTGTCGGAGCTGATCTGGCGCGACTTCTATCAGATGATCCTGTGGCACCACCCGCGGGTCGTC
GATCAGGCCTTCAGGCCGGAGTTTGACCGCATACGCTGGGATGATGCGCCGGCGCTGTTCGATGCCTGGCGCGAGGGCCG
CACCGGCTACCCGATCGTCGATGCCGGCATGCGCCAGCTGCTGCGCAGCGGCTACATGCACAACCGCCTGCGCATGATCG
TTGCGTCCTTCCTGACCAAGGATCTGGGTGTGGACTGGCGGTTGGGCGAGCGGCATTTCGCCGCCCATCTCAACGACTAC
GACCTTGCAGCCAACAACGGAGGCTGGCAGTGGGCCGCGTCCACCGGCTGCGATGCCCAGCCTTATTTCCGCATCTTCAA
TCCGGTGACTCAGTCGCAGAAGTTCGATCCGGAGGGGCGCTTCATCCGCCGCTACGTGCCGGAGCTGGCGCGGGTGCCCG
ACCGCCACATCCACGCCCCGTGGCAGATGGATGCCGCCACCCAGTCCCGCGCTTGCGTACAGATCGGGCGCGATTATCCG
GCACCGGTGGTCGACCACGCCTTGGCCCGCGAGCGCACGTTGGCGCGCTTCGCGGTGACCAAGGGGGGGTGA

Upstream 100 bases:

>100_bases
GTGATGCGTGAGGTTGGCCGATCCGGTATCCATGGCTGCATTTTACGCGCTGATGCGCCGCCCCGCGAGGCCGCTTTGTC
CGCCACGGCCGTTCCGGCCG

Downstream 100 bases:

>100_bases
AGCGAGCGGAGCGGCCGAGGGGCGACCCCCGGCCGTTCAGGCGTGCGCGGCTGCGGCGGTGTAGCCCGCGATCAGCGCAA
GCAGCTCCTCTTCCTGGTAA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSAACVLPLGDNPLPSLPITQCLMTVALVWFRRDLRCVDHAALYHALREHERVYCAFVFDTDILDALPTRADRRVEFIHA
AVVELDRALEALSREAGGSGSGLIVRHGRAQEAIPALAQALGVDAVYVNRDYEPAAIARDRAVAERLAEHGRTLRDFKDQ
VIFDRDEVLTQAGQPFSVFTPYKNAWLRKLDPFYLQAYPIAPRARRLAPKPAGEGVPALGRIGFEPTDLRGLALPTGASG
ARALFGDFCGRIEDYRIARDFPAVKGVSYLSTHLRFGTIPVRELAAYAVHQGGAGAQTWLSELIWRDFYQMILWHHPRVV
DQAFRPEFDRIRWDDAPALFDAWREGRTGYPIVDAGMRQLLRSGYMHNRLRMIVASFLTKDLGVDWRLGERHFAAHLNDY
DLAANNGGWQWAASTGCDAQPYFRIFNPVTQSQKFDPEGRFIRRYVPELARVPDRHIHAPWQMDAATQSRACVQIGRDYP
APVVDHALARERTLARFAVTKGG

Sequences:

>Translated_503_residues
MSAACVLPLGDNPLPSLPITQCLMTVALVWFRRDLRCVDHAALYHALREHERVYCAFVFDTDILDALPTRADRRVEFIHA
AVVELDRALEALSREAGGSGSGLIVRHGRAQEAIPALAQALGVDAVYVNRDYEPAAIARDRAVAERLAEHGRTLRDFKDQ
VIFDRDEVLTQAGQPFSVFTPYKNAWLRKLDPFYLQAYPIAPRARRLAPKPAGEGVPALGRIGFEPTDLRGLALPTGASG
ARALFGDFCGRIEDYRIARDFPAVKGVSYLSTHLRFGTIPVRELAAYAVHQGGAGAQTWLSELIWRDFYQMILWHHPRVV
DQAFRPEFDRIRWDDAPALFDAWREGRTGYPIVDAGMRQLLRSGYMHNRLRMIVASFLTKDLGVDWRLGERHFAAHLNDY
DLAANNGGWQWAASTGCDAQPYFRIFNPVTQSQKFDPEGRFIRRYVPELARVPDRHIHAPWQMDAATQSRACVQIGRDYP
APVVDHALARERTLARFAVTKGG
>Mature_502_residues
SAACVLPLGDNPLPSLPITQCLMTVALVWFRRDLRCVDHAALYHALREHERVYCAFVFDTDILDALPTRADRRVEFIHAA
VVELDRALEALSREAGGSGSGLIVRHGRAQEAIPALAQALGVDAVYVNRDYEPAAIARDRAVAERLAEHGRTLRDFKDQV
IFDRDEVLTQAGQPFSVFTPYKNAWLRKLDPFYLQAYPIAPRARRLAPKPAGEGVPALGRIGFEPTDLRGLALPTGASGA
RALFGDFCGRIEDYRIARDFPAVKGVSYLSTHLRFGTIPVRELAAYAVHQGGAGAQTWLSELIWRDFYQMILWHHPRVVD
QAFRPEFDRIRWDDAPALFDAWREGRTGYPIVDAGMRQLLRSGYMHNRLRMIVASFLTKDLGVDWRLGERHFAAHLNDYD
LAANNGGWQWAASTGCDAQPYFRIFNPVTQSQKFDPEGRFIRRYVPELARVPDRHIHAPWQMDAATQSRACVQIGRDYPA
PVVDHALARERTLARFAVTKGG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=491, Percent_Identity=26.8839103869654, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=29.2682926829268, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI188536103, Length=445, Percent_Identity=28.9887640449438, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI1786926, Length=492, Percent_Identity=40.0406504065041, Blast_Score=313, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324962, Length=515, Percent_Identity=28.7378640776699, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137248, Length=504, Percent_Identity=27.9761904761905, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24585455, Length=504, Percent_Identity=27.9761904761905, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24648152, Length=520, Percent_Identity=26.1538461538462, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 56607; Mature: 56476

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAACVLPLGDNPLPSLPITQCLMTVALVWFRRDLRCVDHAALYHALREHERVYCAFVFD
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
TDILDALPTRADRRVEFIHAAVVELDRALEALSREAGGSGSGLIVRHGRAQEAIPALAQA
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
LGVDAVYVNRDYEPAAIARDRAVAERLAEHGRTLRDFKDQVIFDRDEVLTQAGQPFSVFT
HCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEC
PYKNAWLRKLDPFYLQAYPIAPRARRLAPKPAGEGVPALGRIGFEPTDLRGLALPTGASG
CCHHHHHHHCCCHHEEEECCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCC
ARALFGDFCGRIEDYRIARDFPAVKGVSYLSTHLRFGTIPVRELAAYAVHQGGAGAQTWL
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
SELIWRDFYQMILWHHPRVVDQAFRPEFDRIRWDDAPALFDAWREGRTGYPIVDAGMRQL
HHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LRSGYMHNRLRMIVASFLTKDLGVDWRLGERHFAAHLNDYDLAANNGGWQWAASTGCDAQ
HHCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCEECCCCCEEEECCCCCCCC
PYFRIFNPVTQSQKFDPEGRFIRRYVPELARVPDRHIHAPWQMDAATQSRACVQIGRDYP
CHHHHHCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
APVVDHALARERTLARFAVTKGG
CHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAACVLPLGDNPLPSLPITQCLMTVALVWFRRDLRCVDHAALYHALREHERVYCAFVFD
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
TDILDALPTRADRRVEFIHAAVVELDRALEALSREAGGSGSGLIVRHGRAQEAIPALAQA
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
LGVDAVYVNRDYEPAAIARDRAVAERLAEHGRTLRDFKDQVIFDRDEVLTQAGQPFSVFT
HCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEC
PYKNAWLRKLDPFYLQAYPIAPRARRLAPKPAGEGVPALGRIGFEPTDLRGLALPTGASG
CCHHHHHHHCCCHHEEEECCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCC
ARALFGDFCGRIEDYRIARDFPAVKGVSYLSTHLRFGTIPVRELAAYAVHQGGAGAQTWL
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
SELIWRDFYQMILWHHPRVVDQAFRPEFDRIRWDDAPALFDAWREGRTGYPIVDAGMRQL
HHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LRSGYMHNRLRMIVASFLTKDLGVDWRLGERHFAAHLNDYDLAANNGGWQWAASTGCDAQ
HHCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCEECCCCCEEEECCCCCCCC
PYFRIFNPVTQSQKFDPEGRFIRRYVPELARVPDRHIHAPWQMDAATQSRACVQIGRDYP
CHHHHHCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
APVVDHALARERTLARFAVTKGG
CHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]