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The map label for this gene is pilS [H]

Identifier: 119899646

GI number: 119899646

Start: 3693033

End: 3694586

Strand: Reverse

Name: pilS [H]

Synonym: azo3357

Alternate gene names: 119899646

Gene position: 3694586-3693033 (Counterclockwise)

Preceding gene: 119899647

Following gene: 119899645

Centisome position: 84.43

GC content: 69.82

Gene sequence:

>1554_bases
GTGGTGGGCAGCCTGTCCGCCGACGTCGATCCGTCGCGCCGCCTGTCGCTGCGCTATTTCAATCTCTTCCGGCTCATCGT
CGGCGGCCTCTTCGTCGTCGCCGGCCGCGAGATCGACCTCGGCTCCTATCACCAGTTCGTCTATAACGCGGCGTCCTTCG
CCTATCTGGGCGCGGTGCTGGCGCTTGGCTTTCCCGACATGCAGCGCCGCTTCGGCATCGACCGCGTCATCCTGGTGCAG
GTGGTGGTCGACGTCTGCCTGCTGACGGCCATCATGTGGGCCAGCGGCGGCTACCGCAGCGGCATGCCGGTGCTGATGAT
GGTGATCCTTGCCGGCGCGGGGCTGGTGGCCGAGGGGCGTACGGTGTTGTTCTTCGCCGCGCTCACCACGGTGCTGGTGC
TGGGCGAGAACGTCTGGCGCTATCTCAAGGGCGCGCAGCCGGCGGATTTCTTCCAGGTGGGCATGGCCTGCCTCGGCTTT
TTCGGCATCGCCATCGTCGCCCGGCTGCTGGCGCTGCGCGCCAAGGCCAATGCGTCGCTGGCCGCCGAGCGCGGCCAGGC
GCTGGCGCGTCAGCAGGCTCTGAACGAACGCATCATCCGCGACATGCAGGACGGCGTGATCGTCATCGGCACGGACGGCC
GCGCGCGCCAGGCCAACCCGCGCGCCTGCGCGCTGCTCGGCCTGCAGTCGGTGGAGGGCCTCAACCTGGCCGACGTGGAT
CCCGCGTTTTGCGAGCTGTTCTCGTGCGGCGCGGTGGATAGCCGCCTGCTCCGTCTCGGCCCCGCCGGGCGGCTGCTGCG
CTGTCGCACCGTGTGCGGCGAGCCGGGCAGCGCCGCCGCCGACGCCACCGTGATCTACCTGACTGACTTCGAAGAAGTGC
AGCGCCAGATGCAGCAGGTGAAGCTGGCCGCGCTGGGCCGGCTCACCGCCAGCATGGCGCACGAAATCCGCAATCCGCTG
TCGGCGGTGACCCAGGCCGCGGAACTGCTGACCGAGGAAAAACGCACCGACATGCAGCTGCGGCTGGCGCGCATCATCAA
CGACAACGCCCGCCGCATCGAACGCATGATCCGCGACGTGCTGGCGCTGGGGCGGCGCGAGCAGGCCGAGCCGGAGCCGC
TGGAACTGGCCGGCTACGTCGCCGAACTGATCGAGGCGCTGGCGCTGCGCAGCGCGGACGAGCGCGCCGTGTTCGCGCAG
GACATCCCGCCCGGCCTCACGCTCGCGATCGACCGGGCCCACCTGCACCAGATCCTCGACAACCTGCTCACCAATGCCCG
CCGCTACTGCAGCGGACAGCCCGGTTCGGTCCGCATCCGCGCCAGCGCGCTGCCGGGCGGCCGGGTCGCACTGCACGTGC
GCGACGACGGCCCCGGCATCGACGAGAGCACCCGTGCCAATCTCTTCGAGCCCTTCTTCACTACGCACCCGAAAGGCACC
GGGCTGGGCCTGTATATCGCGCGCGAACTTGCCGAGGCCAACGATGCCACACTCGAACTCGGGCCGGAGCGTCCGGGCGC
CCACTTCGTGCTGACCGGAGACAGCCGCGCATGA

Upstream 100 bases:

>100_bases
TGCGGGGTGCACGTGCCGGAGCGCGAAGGCGTGCGCGACGGCGGGCGTTTCTACTGCGGCGAGGCGCATCGCCGCCTGGG
GCCGGCCCGGGGGCGCTGAG

Downstream 100 bases:

>100_bases
GCCACACCGAACGCCGCACGCGCCCCGCCACCAGCGAGGTGTTGGTCGTCGATGACGAGGAAGACATCCGCGAGCTGATC
GCGCTGTCACTGCTGCGGCT

Product: two component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVGSLSADVDPSRRLSLRYFNLFRLIVGGLFVVAGREIDLGSYHQFVYNAASFAYLGAVLALGFPDMQRRFGIDRVILVQ
VVVDVCLLTAIMWASGGYRSGMPVLMMVILAGAGLVAEGRTVLFFAALTTVLVLGENVWRYLKGAQPADFFQVGMACLGF
FGIAIVARLLALRAKANASLAAERGQALARQQALNERIIRDMQDGVIVIGTDGRARQANPRACALLGLQSVEGLNLADVD
PAFCELFSCGAVDSRLLRLGPAGRLLRCRTVCGEPGSAAADATVIYLTDFEEVQRQMQQVKLAALGRLTASMAHEIRNPL
SAVTQAAELLTEEKRTDMQLRLARIINDNARRIERMIRDVLALGRREQAEPEPLELAGYVAELIEALALRSADERAVFAQ
DIPPGLTLAIDRAHLHQILDNLLTNARRYCSGQPGSVRIRASALPGGRVALHVRDDGPGIDESTRANLFEPFFTTHPKGT
GLGLYIARELAEANDATLELGPERPGAHFVLTGDSRA

Sequences:

>Translated_517_residues
MVGSLSADVDPSRRLSLRYFNLFRLIVGGLFVVAGREIDLGSYHQFVYNAASFAYLGAVLALGFPDMQRRFGIDRVILVQ
VVVDVCLLTAIMWASGGYRSGMPVLMMVILAGAGLVAEGRTVLFFAALTTVLVLGENVWRYLKGAQPADFFQVGMACLGF
FGIAIVARLLALRAKANASLAAERGQALARQQALNERIIRDMQDGVIVIGTDGRARQANPRACALLGLQSVEGLNLADVD
PAFCELFSCGAVDSRLLRLGPAGRLLRCRTVCGEPGSAAADATVIYLTDFEEVQRQMQQVKLAALGRLTASMAHEIRNPL
SAVTQAAELLTEEKRTDMQLRLARIINDNARRIERMIRDVLALGRREQAEPEPLELAGYVAELIEALALRSADERAVFAQ
DIPPGLTLAIDRAHLHQILDNLLTNARRYCSGQPGSVRIRASALPGGRVALHVRDDGPGIDESTRANLFEPFFTTHPKGT
GLGLYIARELAEANDATLELGPERPGAHFVLTGDSRA
>Mature_517_residues
MVGSLSADVDPSRRLSLRYFNLFRLIVGGLFVVAGREIDLGSYHQFVYNAASFAYLGAVLALGFPDMQRRFGIDRVILVQ
VVVDVCLLTAIMWASGGYRSGMPVLMMVILAGAGLVAEGRTVLFFAALTTVLVLGENVWRYLKGAQPADFFQVGMACLGF
FGIAIVARLLALRAKANASLAAERGQALARQQALNERIIRDMQDGVIVIGTDGRARQANPRACALLGLQSVEGLNLADVD
PAFCELFSCGAVDSRLLRLGPAGRLLRCRTVCGEPGSAAADATVIYLTDFEEVQRQMQQVKLAALGRLTASMAHEIRNPL
SAVTQAAELLTEEKRTDMQLRLARIINDNARRIERMIRDVLALGRREQAEPEPLELAGYVAELIEALALRSADERAVFAQ
DIPPGLTLAIDRAHLHQILDNLLTNARRYCSGQPGSVRIRASALPGGRVALHVRDDGPGIDESTRANLFEPFFTTHPKGT
GLGLYIARELAEANDATLELGPERPGAHFVLTGDSRA

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=331, Percent_Identity=28.3987915407855, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=31.7596566523605, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=203, Percent_Identity=29.064039408867, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=230, Percent_Identity=30, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790300, Length=220, Percent_Identity=29.5454545454545, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=27.1551724137931, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081816, Length=194, Percent_Identity=30.4123711340206, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=24.7933884297521, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790346, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=216, Percent_Identity=28.2407407407407, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56015; Mature: 56015

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGSLSADVDPSRRLSLRYFNLFRLIVGGLFVVAGREIDLGSYHQFVYNAASFAYLGAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
ALGFPDMQRRFGIDRVILVQVVVDVCLLTAIMWASGGYRSGMPVLMMVILAGAGLVAEGR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCC
TVLFFAALTTVLVLGENVWRYLKGAQPADFFQVGMACLGFFGIAIVARLLALRAKANASL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AAERGQALARQQALNERIIRDMQDGVIVIGTDGRARQANPRACALLGLQSVEGLNLADVD
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEHEEHHHCCCCCCCCCC
PAFCELFSCGAVDSRLLRLGPAGRLLRCRTVCGEPGSAAADATVIYLTDFEEVQRQMQQV
HHHHHHHHCCCHHHHHHHCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHH
KLAALGRLTASMAHEIRNPLSAVTQAAELLTEEKRTDMQLRLARIINDNARRIERMIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LALGRREQAEPEPLELAGYVAELIEALALRSADERAVFAQDIPPGLTLAIDRAHLHQILD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECHHHHHHHHH
NLLTNARRYCSGQPGSVRIRASALPGGRVALHVRDDGPGIDESTRANLFEPFFTTHPKGT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHCCCCCC
GLGLYIARELAEANDATLELGPERPGAHFVLTGDSRA
CHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure
MVGSLSADVDPSRRLSLRYFNLFRLIVGGLFVVAGREIDLGSYHQFVYNAASFAYLGAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
ALGFPDMQRRFGIDRVILVQVVVDVCLLTAIMWASGGYRSGMPVLMMVILAGAGLVAEGR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCC
TVLFFAALTTVLVLGENVWRYLKGAQPADFFQVGMACLGFFGIAIVARLLALRAKANASL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AAERGQALARQQALNERIIRDMQDGVIVIGTDGRARQANPRACALLGLQSVEGLNLADVD
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEHEEHHHCCCCCCCCCC
PAFCELFSCGAVDSRLLRLGPAGRLLRCRTVCGEPGSAAADATVIYLTDFEEVQRQMQQV
HHHHHHHHCCCHHHHHHHCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHH
KLAALGRLTASMAHEIRNPLSAVTQAAELLTEEKRTDMQLRLARIINDNARRIERMIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LALGRREQAEPEPLELAGYVAELIEALALRSADERAVFAQDIPPGLTLAIDRAHLHQILD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECHHHHHHHHH
NLLTNARRYCSGQPGSVRIRASALPGGRVALHVRDDGPGIDESTRANLFEPFFTTHPKGT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHCCCCCC
GLGLYIARELAEANDATLELGPERPGAHFVLTGDSRA
CHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]