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The map label for this gene is pilR [H]

Identifier: 119899645

GI number: 119899645

Start: 3691660

End: 3693036

Strand: Reverse

Name: pilR [H]

Synonym: azo3356

Alternate gene names: 119899645

Gene position: 3693036-3691660 (Counterclockwise)

Preceding gene: 119899646

Following gene: 119899644

Centisome position: 84.39

GC content: 69.43

Gene sequence:

>1377_bases
ATGAGCCACACCGAACGCCGCACGCGCCCCGCCACCAGCGAGGTGTTGGTCGTCGATGACGAGGAAGACATCCGCGAGCT
GATCGCGCTGTCACTGCTGCGGCTGGGGCTGGCCTGCGATACCGCCGGCACCGTGGCCGAAGCGCGGCAACTGCTCGATA
CCCGCCGCTATCGCCTGTGCCTTACCGACATGCGCCTGCCGGACGGCGACGGGCTGGAGATCGTGGAGACCATACAGGCG
CGCTGCCCCGGGCTGCCGGTCGCCGTCATCACCGCGTTCGGCAGCATCGACACCGCCATCCGCGCGCTCAAGCTCGGCGC
CTTCGACTTCGTCACCAAGCCGGTGGAGCTGAAGGTGCTGCGCGAACTGGTCACCCACGCGCTCAACCTCGATGCCCCCG
CCGCGCCCACGGAAGAAAGCGGCGGACGCCGCCTGATCGGCAACTCGGCCCCGATGGCCCATCTGCGCGCGCAGATCGCC
AAGCTCGCGCGCAATCAGGCGCCGGTGTTCATCCACGGCGAATCGGGCACCGGCAAGGAAGTCATCGCCAACCTGATACA
CGCCCAGGGCGGCCGCGCCGCTGCGCCCTTCGTGCCGGTCAACTGCGGCGCGATCTCGCCCGAGCTGATGGAGAGCGAGT
TCTTCGGCCACCGCAAGGGCAGCTTCACCGGCGCCAGTGCGGACAAGGAGGGTCTGTTCCAGGCGGCCAGCGGCGGCACG
CTGTTTCTGGACGAAGTGGGCGAGTTGCCGCTCGCAATGCAGGTGAAGCTGCTGCGCGCCATCCAGGAGCGCGCCGTGCG
CCCGGTCGGCGGCCATACCGAGGTGCCAGTGGATGTGCGCATCCTCTCCGCCTCCCACCGCGACCTCGCCCAGCTCGTCG
CCGAAGGCAAGTTCCGCCAGGACCTGTTCTTCCGCATCAACGTCATCACCTTGCGGGTGCCGCCACTGCGCGAACGCCCG
GAGGACGTCCCGGACCTCGCCGCCCACATCCTGCAGCGCCTCGCGCAGCGCGAGGCCACCGCCCCGCGCCGGCTGTCGCC
CGCCGCCCTGGCGCGCCTGCAGCAGTACCCCTTTCCCGGCAATGTGCGCGAACTCGAAAACCTGCTCGAGCGCGCCTGCG
CACTATGTGAAGGCGACGAGATCGGCGCGGAGGACGTGGACCTCTACCCCACCGAGTCGCTGACCCACTCGCAATTCAAC
TTCGACCCCATCCGCGACGCAGCGCCGGACGACACCGACGACGGCAGCGACGACGAGCGTATCCGCGTGCTGCGCGCGCT
CGACGAAACCCGCTGGAACCGCTCCGCCGCCGCCCGCAAGCTGGGGATGACGCTGCGGCAACTGCGCTATCGGCTGCAGA
AGTGGGGGATGGAGTAA

Upstream 100 bases:

>100_bases
TGTATATCGCGCGCGAACTTGCCGAGGCCAACGATGCCACACTCGAACTCGGGCCGGAGCGTCCGGGCGCCCACTTCGTG
CTGACCGGAGACAGCCGCGC

Downstream 100 bases:

>100_bases
GGGGCTGATGGTGACGCCGGGCAATGTCAAAAGTGGACATTGGAGGCGTGACAAATGTCATGGGTTGACGTGCCCCACTG
CGAGTCGTGCCGCCGTAAGC

Product: two component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSHTERRTRPATSEVLVVDDEEDIRELIALSLLRLGLACDTAGTVAEARQLLDTRRYRLCLTDMRLPDGDGLEIVETIQA
RCPGLPVAVITAFGSIDTAIRALKLGAFDFVTKPVELKVLRELVTHALNLDAPAAPTEESGGRRLIGNSAPMAHLRAQIA
KLARNQAPVFIHGESGTGKEVIANLIHAQGGRAAAPFVPVNCGAISPELMESEFFGHRKGSFTGASADKEGLFQAASGGT
LFLDEVGELPLAMQVKLLRAIQERAVRPVGGHTEVPVDVRILSASHRDLAQLVAEGKFRQDLFFRINVITLRVPPLRERP
EDVPDLAAHILQRLAQREATAPRRLSPAALARLQQYPFPGNVRELENLLERACALCEGDEIGAEDVDLYPTESLTHSQFN
FDPIRDAAPDDTDDGSDDERIRVLRALDETRWNRSAAARKLGMTLRQLRYRLQKWGME

Sequences:

>Translated_458_residues
MSHTERRTRPATSEVLVVDDEEDIRELIALSLLRLGLACDTAGTVAEARQLLDTRRYRLCLTDMRLPDGDGLEIVETIQA
RCPGLPVAVITAFGSIDTAIRALKLGAFDFVTKPVELKVLRELVTHALNLDAPAAPTEESGGRRLIGNSAPMAHLRAQIA
KLARNQAPVFIHGESGTGKEVIANLIHAQGGRAAAPFVPVNCGAISPELMESEFFGHRKGSFTGASADKEGLFQAASGGT
LFLDEVGELPLAMQVKLLRAIQERAVRPVGGHTEVPVDVRILSASHRDLAQLVAEGKFRQDLFFRINVITLRVPPLRERP
EDVPDLAAHILQRLAQREATAPRRLSPAALARLQQYPFPGNVRELENLLERACALCEGDEIGAEDVDLYPTESLTHSQFN
FDPIRDAAPDDTDDGSDDERIRVLRALDETRWNRSAAARKLGMTLRQLRYRLQKWGME
>Mature_457_residues
SHTERRTRPATSEVLVVDDEEDIRELIALSLLRLGLACDTAGTVAEARQLLDTRRYRLCLTDMRLPDGDGLEIVETIQAR
CPGLPVAVITAFGSIDTAIRALKLGAFDFVTKPVELKVLRELVTHALNLDAPAAPTEESGGRRLIGNSAPMAHLRAQIAK
LARNQAPVFIHGESGTGKEVIANLIHAQGGRAAAPFVPVNCGAISPELMESEFFGHRKGSFTGASADKEGLFQAASGGTL
FLDEVGELPLAMQVKLLRAIQERAVRPVGGHTEVPVDVRILSASHRDLAQLVAEGKFRQDLFFRINVITLRVPPLRERPE
DVPDLAAHILQRLAQREATAPRRLSPAALARLQQYPFPGNVRELENLLERACALCEGDEIGAEDVDLYPTESLTHSQFNF
DPIRDAAPDDTDDGSDDERIRVLRALDETRWNRSAAARKLGMTLRQLRYRLQKWGME

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=41.5555555555556, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=38.0434782608696, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788905, Length=376, Percent_Identity=43.6170212765958, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=37.5, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=42.6791277258567, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=39.4578313253012, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=334, Percent_Identity=39.8203592814371, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789087, Length=316, Percent_Identity=38.2911392405063, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786524, Length=325, Percent_Identity=39.0769230769231, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=34.3465045592705, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=35.8895705521472, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=35.7692307692308, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081858, Length=283, Percent_Identity=29.6819787985866, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786784, Length=101, Percent_Identity=32.6732673267327, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=33.6448598130841, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50364; Mature: 50233

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTERRTRPATSEVLVVDDEEDIRELIALSLLRLGLACDTAGTVAEARQLLDTRRYRLC
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LTDMRLPDGDGLEIVETIQARCPGLPVAVITAFGSIDTAIRALKLGAFDFVTKPVELKVL
HHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHH
RELVTHALNLDAPAAPTEESGGRRLIGNSAPMAHLRAQIAKLARNQAPVFIHGESGTGKE
HHHHHHHHCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHH
VIANLIHAQGGRAAAPFVPVNCGAISPELMESEFFGHRKGSFTGASADKEGLFQAASGGT
HHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHEECCCCE
LFLDEVGELPLAMQVKLLRAIQERAVRPVGGHTEVPVDVRILSASHRDLAQLVAEGKFRQ
EEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHH
DLFFRINVITLRVPPLRERPEDVPDLAAHILQRLAQREATAPRRLSPAALARLQQYPFPG
CEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
NVRELENLLERACALCEGDEIGAEDVDLYPTESLTHSQFNFDPIRDAAPDDTDDGSDDER
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IRVLRALDETRWNRSAAARKLGMTLRQLRYRLQKWGME
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHTERRTRPATSEVLVVDDEEDIRELIALSLLRLGLACDTAGTVAEARQLLDTRRYRLC
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LTDMRLPDGDGLEIVETIQARCPGLPVAVITAFGSIDTAIRALKLGAFDFVTKPVELKVL
HHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHH
RELVTHALNLDAPAAPTEESGGRRLIGNSAPMAHLRAQIAKLARNQAPVFIHGESGTGKE
HHHHHHHHCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHH
VIANLIHAQGGRAAAPFVPVNCGAISPELMESEFFGHRKGSFTGASADKEGLFQAASGGT
HHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHEECCCCE
LFLDEVGELPLAMQVKLLRAIQERAVRPVGGHTEVPVDVRILSASHRDLAQLVAEGKFRQ
EEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHH
DLFFRINVITLRVPPLRERPEDVPDLAAHILQRLAQREATAPRRLSPAALARLQQYPFPG
CEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
NVRELENLLERACALCEGDEIGAEDVDLYPTESLTHSQFNFDPIRDAAPDDTDDGSDDER
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IRVLRALDETRWNRSAAARKLGMTLRQLRYRLQKWGME
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]