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The map label for this gene is asn [H]

Identifier: 119899536

GI number: 119899536

Start: 3578572

End: 3580368

Strand: Direct

Name: asn [H]

Synonym: azo3247

Alternate gene names: 119899536

Gene position: 3578572-3580368 (Clockwise)

Preceding gene: 119899535

Following gene: 119899537

Centisome position: 81.78

GC content: 67.61

Gene sequence:

>1797_bases
GTGCTCGAGCAGTTCAGCGGCATCCTTCGCCGCAGCCCAAACCCTGACCCGAGCGCCGCATCCGGCACGGCGCTGGAACG
CGTCGACCTCGGCAGGGCCACGGTGGCATACCGCCCCGCCATCGCCGTCGCCCGCGACGGCACCGGACTGTGCATCGCGC
TCGGCCGGCCGCGCTTTGCCGACCCAGCGCTCCAGGCGATTGCCGCACAGCAGGGGCCGGCCGTGGCCTGGCTTCAGGCC
CTGCGCGACCAGGGCGAATCGGTGATTCCTTCCGTGCGCGGCCGCTTCAACGTCGTCTTCATCGAACGCGACGGCGCCGA
GGCGCTGTTTGCCACCGACCGTTTCGGCACCTGGCCCCTGTGCTATGCGGAACAGGACGGCGCGCTCCATTTCGCCGACC
GCGCCGACGCCTTGCCCGGCCCCGGGCGCCGGCTGTCGGCACAGGCCGTGTACGAGTACCTGTTCTTCCACATGATCCCG
GCCCCCACGACGATCTTCGAGGGCGTCGCGCGACTGCCCGGCGGGCATGTGCTGCAGTGGAAAGACGGCCGCGCAACGTG
CCGGGCGTGGTGGCAACCACGTTTCGACGAACGCGAACGGCCCAGCTTCGAATCCGCGAAACGGCAGTTCCTGGACATCG
TCGAGAACGCCGTGCGGCGCGACGCGGAAGGCCCGGTCGGCTGCTTCCTGTCGGGCGGCACCGACAGCTCCACCGTCACC
GGCATGCTGTGCAAGGTCCTCGGCCGGCCCGCGCGCAGCTACTCCATCGGGTTCGACGCAAGCGGCTACGACGAGATGGA
GTACGCTCGTATCGCCGCCCGTCATTTCGGCGCCGACCACCACGAGTACTACGTTACCCCGGACGATCTGCTGGACGGGA
TTCCCCGCGTCGCGGTCCATTACGACCAGCCCTTCGGCAACTCCTCCGCGGTGCCGGCCTGGATCTGCGCGTCCCGCGCG
CAAGACGAAGGCGTGGCACGACTGCTGGCGGGCGACGGGGGCGACGAGTTGTTCGGCGGCAATACCCGCTACGCCAAGCA
GCGTGTTTTCGGGTGGTACGACAGCGTTCCCGGGCTGGTGCGGCGCGGCATGCTCGAGCCGGTGCTCGCCCTTCCCGGGA
TGGGCCGCATCCCGCTGATGAAGAAGGGGGTGAGCTACGTCGAACAGGCCCGTGTGCCGATGCCGGATCGCATGCACATG
TACAACATGCTGCTGCGACTCGGCACCCGTGAGGTTTTCGAAGCCGACTTTCTGGCCCGGATTGACGTCGATCGCCCGCA
GCAGCAGCAGCGCGAGGTCTGGCAGGCAAGCTCGGCGCGCAGCCAGGTCAATCGCATGCTGGCGTACGACTGGAAATACA
CCCTGGCGGACAACGACCTGCCGAAGGTCATCGGCACCACACAACTCGCCGGCGTCGATGTGGCCTTTCCCCTGCTCAGC
GACGAATTGCTCGACTTTTCGCTCACACTGCCGCCCGACTGGAAGCTGCGCGGACTGACCCTGCGCTGGTTTTTCAAGGA
GGCCTTGCGCGGTTTCCTGCCGGACCAGATCATCACGAAGAAGAAGCACGGGTTCGGCCTGCCGTTCGGCGTCTGGGCAT
GCGCCCACGCCGGACTGAAGGCCCAGGCGCACGACGCGCTGCGTAGCCTTGCCGCGCGCGGCATCGTCCGCCCCCGTTTC
ATCGACGAACTCTTCACCACTCACCTGCCAGCGCACCCCGGTTACTACGGCGAAATGGTATGGATCCTGATGATGCTGGA
GTTCTGGCTGCGCGCCCACGACGGCAGCACCCTCTGA

Upstream 100 bases:

>100_bases
CTGAGGCGCGCCGTCCATACACGCGAACAAGGCGGCATGAATCACTCATCATTCCGCCCCCAAAGAACCTAGGCCGGCCG
CCAGACCGGGGGAGTTCATT

Downstream 100 bases:

>100_bases
CCCCGGGGACGATGCGCACGACGCCCCCATTCGCGGAAACCGAAGGCCCCGCCATGACCGAAGCCCGACCGACGCCCACC
CATACGCCCATGACCCAGTT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MLEQFSGILRRSPNPDPSAASGTALERVDLGRATVAYRPAIAVARDGTGLCIALGRPRFADPALQAIAAQQGPAVAWLQA
LRDQGESVIPSVRGRFNVVFIERDGAEALFATDRFGTWPLCYAEQDGALHFADRADALPGPGRRLSAQAVYEYLFFHMIP
APTTIFEGVARLPGGHVLQWKDGRATCRAWWQPRFDERERPSFESAKRQFLDIVENAVRRDAEGPVGCFLSGGTDSSTVT
GMLCKVLGRPARSYSIGFDASGYDEMEYARIAARHFGADHHEYYVTPDDLLDGIPRVAVHYDQPFGNSSAVPAWICASRA
QDEGVARLLAGDGGDELFGGNTRYAKQRVFGWYDSVPGLVRRGMLEPVLALPGMGRIPLMKKGVSYVEQARVPMPDRMHM
YNMLLRLGTREVFEADFLARIDVDRPQQQQREVWQASSARSQVNRMLAYDWKYTLADNDLPKVIGTTQLAGVDVAFPLLS
DELLDFSLTLPPDWKLRGLTLRWFFKEALRGFLPDQIITKKKHGFGLPFGVWACAHAGLKAQAHDALRSLAARGIVRPRF
IDELFTTHLPAHPGYYGEMVWILMMLEFWLRAHDGSTL

Sequences:

>Translated_598_residues
MLEQFSGILRRSPNPDPSAASGTALERVDLGRATVAYRPAIAVARDGTGLCIALGRPRFADPALQAIAAQQGPAVAWLQA
LRDQGESVIPSVRGRFNVVFIERDGAEALFATDRFGTWPLCYAEQDGALHFADRADALPGPGRRLSAQAVYEYLFFHMIP
APTTIFEGVARLPGGHVLQWKDGRATCRAWWQPRFDERERPSFESAKRQFLDIVENAVRRDAEGPVGCFLSGGTDSSTVT
GMLCKVLGRPARSYSIGFDASGYDEMEYARIAARHFGADHHEYYVTPDDLLDGIPRVAVHYDQPFGNSSAVPAWICASRA
QDEGVARLLAGDGGDELFGGNTRYAKQRVFGWYDSVPGLVRRGMLEPVLALPGMGRIPLMKKGVSYVEQARVPMPDRMHM
YNMLLRLGTREVFEADFLARIDVDRPQQQQREVWQASSARSQVNRMLAYDWKYTLADNDLPKVIGTTQLAGVDVAFPLLS
DELLDFSLTLPPDWKLRGLTLRWFFKEALRGFLPDQIITKKKHGFGLPFGVWACAHAGLKAQAHDALRSLAARGIVRPRF
IDELFTTHLPAHPGYYGEMVWILMMLEFWLRAHDGSTL
>Mature_598_residues
MLEQFSGILRRSPNPDPSAASGTALERVDLGRATVAYRPAIAVARDGTGLCIALGRPRFADPALQAIAAQQGPAVAWLQA
LRDQGESVIPSVRGRFNVVFIERDGAEALFATDRFGTWPLCYAEQDGALHFADRADALPGPGRRLSAQAVYEYLFFHMIP
APTTIFEGVARLPGGHVLQWKDGRATCRAWWQPRFDERERPSFESAKRQFLDIVENAVRRDAEGPVGCFLSGGTDSSTVT
GMLCKVLGRPARSYSIGFDASGYDEMEYARIAARHFGADHHEYYVTPDDLLDGIPRVAVHYDQPFGNSSAVPAWICASRA
QDEGVARLLAGDGGDELFGGNTRYAKQRVFGWYDSVPGLVRRGMLEPVLALPGMGRIPLMKKGVSYVEQARVPMPDRMHM
YNMLLRLGTREVFEADFLARIDVDRPQQQQREVWQASSARSQVNRMLAYDWKYTLADNDLPKVIGTTQLAGVDVAFPLLS
DELLDFSLTLPPDWKLRGLTLRWFFKEALRGFLPDQIITKKKHGFGLPFGVWACAHAGLKAQAHDALRSLAARGIVRPRF
IDELFTTHLPAHPGYYGEMVWILMMLEFWLRAHDGSTL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=366, Percent_Identity=23.7704918032787, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66571; Mature: 66571

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQFSGILRRSPNPDPSAASGTALERVDLGRATVAYRPAIAVARDGTGLCIALGRPRFA
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEECCEEEEEECCCEEEEEECCCCCC
DPALQAIAAQQGPAVAWLQALRDQGESVIPSVRGRFNVVFIERDGAEALFATDRFGTWPL
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCE
CYAEQDGALHFADRADALPGPGRRLSAQAVYEYLFFHMIPAPTTIFEGVARLPGGHVLQW
EEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEE
KDGRATCRAWWQPRFDERERPSFESAKRQFLDIVENAVRRDAEGPVGCFLSGGTDSSTVT
CCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
GMLCKVLGRPARSYSIGFDASGYDEMEYARIAARHFGADHHEYYVTPDDLLDGIPRVAVH
HHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCEEEE
YDQPFGNSSAVPAWICASRAQDEGVARLLAGDGGDELFGGNTRYAKQRVFGWYDSVPGLV
ECCCCCCCCCCHHHHHHHCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
RRGMLEPVLALPGMGRIPLMKKGVSYVEQARVPMPDRMHMYNMLLRLGTREVFEADFLAR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHE
IDVDRPQQQQREVWQASSARSQVNRMLAYDWKYTLADNDLPKVIGTTQLAGVDVAFPLLS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHCCHHHCCCEEEHHHHH
DELLDFSLTLPPDWKLRGLTLRWFFKEALRGFLPDQIITKKKHGFGLPFGVWACAHAGLK
HHHHCEEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHCCCC
AQAHDALRSLAARGIVRPRFIDELFTTHLPAHPGYYGEMVWILMMLEFWLRAHDGSTL
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLEQFSGILRRSPNPDPSAASGTALERVDLGRATVAYRPAIAVARDGTGLCIALGRPRFA
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEECCEEEEEECCCEEEEEECCCCCC
DPALQAIAAQQGPAVAWLQALRDQGESVIPSVRGRFNVVFIERDGAEALFATDRFGTWPL
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCE
CYAEQDGALHFADRADALPGPGRRLSAQAVYEYLFFHMIPAPTTIFEGVARLPGGHVLQW
EEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEE
KDGRATCRAWWQPRFDERERPSFESAKRQFLDIVENAVRRDAEGPVGCFLSGGTDSSTVT
CCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
GMLCKVLGRPARSYSIGFDASGYDEMEYARIAARHFGADHHEYYVTPDDLLDGIPRVAVH
HHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCEEEE
YDQPFGNSSAVPAWICASRAQDEGVARLLAGDGGDELFGGNTRYAKQRVFGWYDSVPGLV
ECCCCCCCCCCHHHHHHHCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
RRGMLEPVLALPGMGRIPLMKKGVSYVEQARVPMPDRMHMYNMLLRLGTREVFEADFLAR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHE
IDVDRPQQQQREVWQASSARSQVNRMLAYDWKYTLADNDLPKVIGTTQLAGVDVAFPLLS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHCCHHHCCCEEEHHHHH
DELLDFSLTLPPDWKLRGLTLRWFFKEALRGFLPDQIITKKKHGFGLPFGVWACAHAGLK
HHHHCEEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHCCCC
AQAHDALRSLAARGIVRPRFIDELFTTHLPAHPGYYGEMVWILMMLEFWLRAHDGSTL
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]