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The map label for this gene is fadD4 [H]

Identifier: 119899535

GI number: 119899535

Start: 3576895

End: 3578475

Strand: Direct

Name: fadD4 [H]

Synonym: azo3246

Alternate gene names: 119899535

Gene position: 3576895-3578475 (Clockwise)

Preceding gene: 119899534

Following gene: 119899536

Centisome position: 81.74

GC content: 68.44

Gene sequence:

>1581_bases
ATGAATGCGACCCATCTGCTCCACGACCTGATTGCAGCTTCCGCCGAGCGCAGTCCGGACGCCGCGGCCCTGACGGCCGG
CGGCGAGACGCTCGCCTACAGCGCACTCGCGCGCGACGTGGCGCAATGCGCCGCCGGCCTGCTCGCACTCGGCCTGGACC
GCGGCGAACGCGTCGGCATCTACCTCGACAAACGGATCGAAACCGTCACCACCTTCTTCGGCGCCACCGCCGCGGGCGGC
GTCTTCGTACCCGTCAATCCCATCGTGAAAGCCGAACAGGTGGGTTACATCCTGCAGGACTGCAACGTGCGGGTGCTGGT
CACCGGGGTGGAACGCTTCGCCGCGCTGCGCGACACCCTGTCCGCGTGTCACGACCTCCGCCACGTGGTGCTCGTCGGCA
ACGCCGCCGAGTTGCCCACGCTGCCGGGCGCGTCGGTCCACCGCTGGAGCGAACTTCTCGACGCCGCGCCGCGCGCCGGC
CATCGGGTGGTGGATGCGGACATGGCCGCCATCCTCTACACCTCGGGCAGCACCGGGCGCCCCAAGGGCGTCGTGCTGTC
CCATCGCAACATGGTCGCCGGCGCCAAGAGCGTCGCCCAGTATCTCGACAACGGCGCGCACGACACGCTGCTCGCGGCAC
TGCCCTTATCGTTCGACGCGGGCTTCTCCCAGCTCACCACCGCGTTCCACTCGGGCGCCAGGGTCGTGCTGTTGAACTAC
CTGATGCCGCGCGACGTGCTGAAGGCGATCGAGCGCGAGCGCGTCACCGGCCTCACCGCGGTGCCACCGCTGTGGATCCA
GTTGTCGCAACTCGAATGGTCGGCTTCGATTACCGAGCACCTGCGCTACATCGCCAATACGGGCGGACGGATGCCGCTGG
AAACGCTCACCCGCCTGCGGGCCGCGCTGCCCCGGACCAAACCCTTCCTGATGTACGGGCTGACCGAAGCATTTCGCGCC
ACCTTCCTGCCCCCCGACGAAGTCGACCGCCGCCCCGACTCGATCGGCAAGTCGATCCCCAATTCCGAGGTGCTGGTGCT
GCGTGAGGACGGCACGGAATGCGCACCGAACGAACCGGGCGAACTCGTGCAGCGCGGCGCGCTGGTGGCGATGGGCTACT
GGAACGACCCCGAGAAGACGGCAGAACGCTTCAAGCCGCTGCCGGCCCACGCGCCAGGACGCCAGAGCGGGCTGGTGTTG
CCCGAAATCGCGGTGTTCTCCGGCGACAACGTCCGCCGCGACGAAGAAGGCTTCCTGTACTTCATCGGCCGTCGGGACGA
GATGATCAAGACCTCGGGCTACCGGGTCAGCCCGACCGAGGTGGAGGAGATCGTCTACGCCACCCGTCTGGTGGGCGAGT
GTGCGGCCTTCGGCGTGGCGCACGACACCCTCGGACAAAGCATCACCGTCATCGTCACGCCGCCGCCCGGCGGCACGGTG
GATGCAGCGACGCTGCTGGCCGAGTGCCGTGCACGCATGCCGGCCTACATGGTGCCGACCAGGATCGACGTGCGTTCCGG
TCCCCTGCCCCGCAATCCGAACGGCAAGATCGACCGCAAGGCCCTCGCAAGCGAGGCCTGA

Upstream 100 bases:

>100_bases
CTCGAATGCCGCAGATCGGGCTTACGCCACACAATTATTCGCACTTGATCCGCTAAACTCGCGGCTTGCGCTGATCATCC
ATACGGTATTTTCGACTGTC

Downstream 100 bases:

>100_bases
GGCGCGCCGTCCATACACGCGAACAAGGCGGCATGAATCACTCATCATTCCGCCCCCAAAGAACCTAGGCCGGCCGCCAG
ACCGGGGGAGTTCATTGTGC

Product: long-chain-fatty-acid-CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNATHLLHDLIAASAERSPDAAALTAGGETLAYSALARDVAQCAAGLLALGLDRGERVGIYLDKRIETVTTFFGATAAGG
VFVPVNPIVKAEQVGYILQDCNVRVLVTGVERFAALRDTLSACHDLRHVVLVGNAAELPTLPGASVHRWSELLDAAPRAG
HRVVDADMAAILYTSGSTGRPKGVVLSHRNMVAGAKSVAQYLDNGAHDTLLAALPLSFDAGFSQLTTAFHSGARVVLLNY
LMPRDVLKAIERERVTGLTAVPPLWIQLSQLEWSASITEHLRYIANTGGRMPLETLTRLRAALPRTKPFLMYGLTEAFRA
TFLPPDEVDRRPDSIGKSIPNSEVLVLREDGTECAPNEPGELVQRGALVAMGYWNDPEKTAERFKPLPAHAPGRQSGLVL
PEIAVFSGDNVRRDEEGFLYFIGRRDEMIKTSGYRVSPTEVEEIVYATRLVGECAAFGVAHDTLGQSITVIVTPPPGGTV
DAATLLAECRARMPAYMVPTRIDVRSGPLPRNPNGKIDRKALASEA

Sequences:

>Translated_526_residues
MNATHLLHDLIAASAERSPDAAALTAGGETLAYSALARDVAQCAAGLLALGLDRGERVGIYLDKRIETVTTFFGATAAGG
VFVPVNPIVKAEQVGYILQDCNVRVLVTGVERFAALRDTLSACHDLRHVVLVGNAAELPTLPGASVHRWSELLDAAPRAG
HRVVDADMAAILYTSGSTGRPKGVVLSHRNMVAGAKSVAQYLDNGAHDTLLAALPLSFDAGFSQLTTAFHSGARVVLLNY
LMPRDVLKAIERERVTGLTAVPPLWIQLSQLEWSASITEHLRYIANTGGRMPLETLTRLRAALPRTKPFLMYGLTEAFRA
TFLPPDEVDRRPDSIGKSIPNSEVLVLREDGTECAPNEPGELVQRGALVAMGYWNDPEKTAERFKPLPAHAPGRQSGLVL
PEIAVFSGDNVRRDEEGFLYFIGRRDEMIKTSGYRVSPTEVEEIVYATRLVGECAAFGVAHDTLGQSITVIVTPPPGGTV
DAATLLAECRARMPAYMVPTRIDVRSGPLPRNPNGKIDRKALASEA
>Mature_526_residues
MNATHLLHDLIAASAERSPDAAALTAGGETLAYSALARDVAQCAAGLLALGLDRGERVGIYLDKRIETVTTFFGATAAGG
VFVPVNPIVKAEQVGYILQDCNVRVLVTGVERFAALRDTLSACHDLRHVVLVGNAAELPTLPGASVHRWSELLDAAPRAG
HRVVDADMAAILYTSGSTGRPKGVVLSHRNMVAGAKSVAQYLDNGAHDTLLAALPLSFDAGFSQLTTAFHSGARVVLLNY
LMPRDVLKAIERERVTGLTAVPPLWIQLSQLEWSASITEHLRYIANTGGRMPLETLTRLRAALPRTKPFLMYGLTEAFRA
TFLPPDEVDRRPDSIGKSIPNSEVLVLREDGTECAPNEPGELVQRGALVAMGYWNDPEKTAERFKPLPAHAPGRQSGLVL
PEIAVFSGDNVRRDEEGFLYFIGRRDEMIKTSGYRVSPTEVEEIVYATRLVGECAAFGVAHDTLGQSITVIVTPPPGGTV
DAATLLAECRARMPAYMVPTRIDVRSGPLPRNPNGKIDRKALASEA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=559, Percent_Identity=26.2969588550984, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI187761345, Length=507, Percent_Identity=27.6134122287968, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI187761343, Length=507, Percent_Identity=27.6134122287968, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=25.9398496240602, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=25.9398496240602, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=25.891181988743, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI115511026, Length=501, Percent_Identity=25.1497005988024, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI38505220, Length=552, Percent_Identity=24.0942028985507, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4758332, Length=508, Percent_Identity=24.8031496062992, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI12669909, Length=508, Percent_Identity=24.8031496062992, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI42544132, Length=541, Percent_Identity=21.4417744916821, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI122937307, Length=489, Percent_Identity=22.4948875255624, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI83745141, Length=485, Percent_Identity=22.8865979381443, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI195947378, Length=442, Percent_Identity=25.1131221719457, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=28.8014311270125, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI221142682, Length=508, Percent_Identity=27.9527559055118, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693145, Length=547, Percent_Identity=25.5941499085923, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1786801, Length=551, Percent_Identity=24.6823956442831, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=24.7619047619048, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=22.6305609284333, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788595, Length=355, Percent_Identity=26.1971830985915, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560308, Length=553, Percent_Identity=26.2206148282098, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=22.8260869565217, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=22.8260869565217, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=22.8260869565217, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=23.8921001926782, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=528, Percent_Identity=24.4318181818182, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=23.9089184060721, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=512, Percent_Identity=22.8515625, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=486, Percent_Identity=22.8395061728395, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=536, Percent_Identity=22.2014925373134, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=541, Percent_Identity=23.2902033271719, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=371, Percent_Identity=23.989218328841, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=537, Percent_Identity=24.2085661080074, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=537, Percent_Identity=24.2085661080074, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=505, Percent_Identity=23.7623762376238, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6322182, Length=368, Percent_Identity=25.5434782608696, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=25.7657657657658, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859661, Length=490, Percent_Identity=25.5102040816327, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356441, Length=364, Percent_Identity=25.2747252747253, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24656500, Length=518, Percent_Identity=23.7451737451737, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922652, Length=518, Percent_Identity=23.9382239382239, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648260, Length=363, Percent_Identity=23.6914600550964, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648253, Length=356, Percent_Identity=25, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17933690, Length=486, Percent_Identity=23.0452674897119, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648255, Length=356, Percent_Identity=25, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355181, Length=537, Percent_Identity=22.5325884543762, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI281365686, Length=328, Percent_Identity=24.390243902439, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21358303, Length=334, Percent_Identity=23.3532934131737, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356947, Length=489, Percent_Identity=21.881390593047, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653035, Length=432, Percent_Identity=24.537037037037, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=25.6130790190736, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648257, Length=463, Percent_Identity=24.1900647948164, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20130353, Length=554, Percent_Identity=24.0072202166065, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573631, Length=554, Percent_Identity=24.0072202166065, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921316, Length=504, Percent_Identity=24.0079365079365, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161076582, Length=444, Percent_Identity=23.6486486486486, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56605; Mature: 56605

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATHLLHDLIAASAERSPDAAALTAGGETLAYSALARDVAQCAAGLLALGLDRGERVGI
CCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
YLDKRIETVTTFFGATAAGGVFVPVNPIVKAEQVGYILQDCNVRVLVTGVERFAALRDTL
EECCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
SACHDLRHVVLVGNAAELPTLPGASVHRWSELLDAAPRAGHRVVDADMAAILYTSGSTGR
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCC
PKGVVLSHRNMVAGAKSVAQYLDNGAHDTLLAALPLSFDAGFSQLTTAFHSGARVVLLNY
CCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHCCCCEEEHHH
LMPRDVLKAIERERVTGLTAVPPLWIQLSQLEWSASITEHLRYIANTGGRMPLETLTRLR
HCCHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AALPRTKPFLMYGLTEAFRATFLPPDEVDRRPDSIGKSIPNSEVLVLREDGTECAPNEPG
HHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCH
ELVQRGALVAMGYWNDPEKTAERFKPLPAHAPGRQSGLVLPEIAVFSGDNVRRDEEGFLY
HHHHCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCEECCEEEEECCCCCCCCCCCEEE
FIGRRDEMIKTSGYRVSPTEVEEIVYATRLVGECAAFGVAHDTLGQSITVIVTPPPGGTV
EEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCCCH
DAATLLAECRARMPAYMVPTRIDVRSGPLPRNPNGKIDRKALASEA
HHHHHHHHHHHCCCCEECCCEEECCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MNATHLLHDLIAASAERSPDAAALTAGGETLAYSALARDVAQCAAGLLALGLDRGERVGI
CCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
YLDKRIETVTTFFGATAAGGVFVPVNPIVKAEQVGYILQDCNVRVLVTGVERFAALRDTL
EECCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
SACHDLRHVVLVGNAAELPTLPGASVHRWSELLDAAPRAGHRVVDADMAAILYTSGSTGR
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCC
PKGVVLSHRNMVAGAKSVAQYLDNGAHDTLLAALPLSFDAGFSQLTTAFHSGARVVLLNY
CCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHCCCCEEEHHH
LMPRDVLKAIERERVTGLTAVPPLWIQLSQLEWSASITEHLRYIANTGGRMPLETLTRLR
HCCHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AALPRTKPFLMYGLTEAFRATFLPPDEVDRRPDSIGKSIPNSEVLVLREDGTECAPNEPG
HHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCH
ELVQRGALVAMGYWNDPEKTAERFKPLPAHAPGRQSGLVLPEIAVFSGDNVRRDEEGFLY
HHHHCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCEECCEEEEECCCCCCCCCCCEEE
FIGRRDEMIKTSGYRVSPTEVEEIVYATRLVGECAAFGVAHDTLGQSITVIVTPPPGGTV
EEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCCCH
DAATLLAECRARMPAYMVPTRIDVRSGPLPRNPNGKIDRKALASEA
HHHHHHHHHHHCCCCEECCCEEECCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]