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The map label for this gene is pilD [H]

Identifier: 119899525

GI number: 119899525

Start: 3567612

End: 3568463

Strand: Direct

Name: pilD [H]

Synonym: azo3236

Alternate gene names: 119899525

Gene position: 3567612-3568463 (Clockwise)

Preceding gene: 119899524

Following gene: 119899526

Centisome position: 81.53

GC content: 64.91

Gene sequence:

>852_bases
ATGCCTGAATACCTTCGCGACCCTGTCGCCTTTGCTGCCGTCGCGACCCTGCTCGGCCTCTTTGTCGGCAGCTTCCTCAA
CGTCGTGATCCATCGGCTGCCGCGCATGATGGAACGGGAGTGGCACGCCCAGGCGGCCGAACTGCGGGGCGAAGAACCCG
AGGCCGGCGAGCCGTTCAACCTCGCGACACCGCGTTCGCGCTGCCCCCACTGCGGGCATCTGATTGGCGCGATCGAGAAC
ATTCCGGTGGTCAGCTATCTGGTGCTGCGCGGTCGCTGCCGCCACTGCAGCGCGCCGATCGGAAAGCGCTACCCGCTCAT
CGAGACGTTCTCCGCTGTCGCGTCCGCCTACGTCGCGTGGCATTTCGGCCCGGGCTTGGCCGCCGTCGGCGGCCTGATCT
TCCTGTGGTCAATGATCGCACTGGCCTTCATCGATCTCGACACACAACTGCTGCCGGACCAGATCACACTCCCGCTGCTG
TGGCTCGGCCTCGCGTTCAATCTTGCTTCCACCTATGTGCCGCTCAGCGCCGCCGTCGTCGGTGCGATGCTGGGTTACTT
GTCGCTATGGTCGGTTTACTGGCTATTCAAACTCGTCACCGGCAAGGAGGGAATGGGTTACGGCGACTTCAAGCTGCTGG
CAGCAATCGGCGCGTGGCTGGGCTGGCAAGTGCTGCCGCTCACCATCCTGCTGTCCTCGCTGGTCGGCGCGGCGGTCGGC
ATCAGCCTCATCCTGTTCGCCCGCCATGGCCGCAACGTTCCGATTCCATTCGGGCCCTATCTCGCCGCCGCGGGCATCCT
CGCCTTGTTGTGGGGCGAGCAGATCACCGCGCGCTATCTCGGCCAGTTCTGA

Upstream 100 bases:

>100_bases
CGGCCTTGTGGTGGCCATGTATCTTCCGATCTTCAAGCTGGGCTCCATCGTCTAGCGCCGGCTCCACCCTCAAGAGCCGG
CTCATCGCCGGCTACCCTCC

Downstream 100 bases:

>100_bases
TACGCCCAGCGCCTGGGGTCTTGATTTTCCAGCACGGCGCCCACATATCCGCGTCGAGCCGCGGCTGCATCACTGCCGGC
CCGGCGCTTGCTTTTTCCCG

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPEYLRDPVAFAAVATLLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEEPEAGEPFNLATPRSRCPHCGHLIGAIEN
IPVVSYLVLRGRCRHCSAPIGKRYPLIETFSAVASAYVAWHFGPGLAAVGGLIFLWSMIALAFIDLDTQLLPDQITLPLL
WLGLAFNLASTYVPLSAAVVGAMLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAAVG
ISLILFARHGRNVPIPFGPYLAAAGILALLWGEQITARYLGQF

Sequences:

>Translated_283_residues
MPEYLRDPVAFAAVATLLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEEPEAGEPFNLATPRSRCPHCGHLIGAIEN
IPVVSYLVLRGRCRHCSAPIGKRYPLIETFSAVASAYVAWHFGPGLAAVGGLIFLWSMIALAFIDLDTQLLPDQITLPLL
WLGLAFNLASTYVPLSAAVVGAMLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAAVG
ISLILFARHGRNVPIPFGPYLAAAGILALLWGEQITARYLGQF
>Mature_282_residues
PEYLRDPVAFAAVATLLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEEPEAGEPFNLATPRSRCPHCGHLIGAIENI
PVVSYLVLRGRCRHCSAPIGKRYPLIETFSAVASAYVAWHFGPGLAAVGGLIFLWSMIALAFIDLDTQLLPDQITLPLLW
LGLAFNLASTYVPLSAAVVGAMLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAAVGI
SLILFARHGRNVPIPFGPYLAAAGILALLWGEQITARYLGQF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=270, Percent_Identity=40.7407407407407, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789732, Length=148, Percent_Identity=50, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30784; Mature: 30653

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYLRDPVAFAAVATLLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEEPEAGEPFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LATPRSRCPHCGHLIGAIENIPVVSYLVLRGRCRHCSAPIGKRYPLIETFSAVASAYVAW
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HFGPGLAAVGGLIFLWSMIALAFIDLDTQLLPDQITLPLLWLGLAFNLASTYVPLSAAVV
HCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAAVG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLILFARHGRNVPIPFGPYLAAAGILALLWGEQITARYLGQF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEYLRDPVAFAAVATLLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEEPEAGEPFN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LATPRSRCPHCGHLIGAIENIPVVSYLVLRGRCRHCSAPIGKRYPLIETFSAVASAYVAW
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HFGPGLAAVGGLIFLWSMIALAFIDLDTQLLPDQITLPLLWLGLAFNLASTYVPLSAAVV
HCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAAVG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLILFARHGRNVPIPFGPYLAAAGILALLWGEQITARYLGQF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA