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The map label for this gene is pldA [H]

Identifier: 119899499

GI number: 119899499

Start: 3537116

End: 3538288

Strand: Reverse

Name: pldA [H]

Synonym: azo3210

Alternate gene names: 119899499

Gene position: 3538288-3537116 (Counterclockwise)

Preceding gene: 119899500

Following gene: 119899498

Centisome position: 80.86

GC content: 70.5

Gene sequence:

>1173_bases
TTGAGCCTACGGCCCTGCTTGTCCTTGTTGTGCCTGCTGGTGGGCGGCGCGGCGGTTGCGGCGCCGGCCGCCGCGCCGGA
TGCGTGGTTGCTGGCCAGTCCCGAGCCGCGCGTGGTTCCGGGGCAACCCTTCGAGGTGATCGTGATCGCCCCGTCCGCGC
TGGGCGAATGGCCGGCGACACTGCCGGCGCAGATCGAGCTTGCGGCGCCCGGCCCGCGGATTGCCATCGACCTGGTGCCG
GCCAACGTGCCGGCGCAGGGCGGCCAGCGCCGCTACATCGGCCACTGGCCGCGCGAGGTGGTGGGCGTGGCGACGCTGTC
GCTGCGCGAGGCGGCCTCTGCGCGGCTGCTGATGGACGCCGCGGCGGCGAGCCGCAGTCCGGTTGAGGTCGCGCGCTCGG
TGCCGGTCGACGCGGCAGCCGATGTCGCCGCGCAGGCGCCGGGCGCGGAGCCGGCCCAGCCGGCCGCGCTCGGCTTTCAC
GAGCCGATGTACTTCGTCGCCGGGGGGCGCAATCCGCGCTCGGCGCGCTATCAGCTCAGCTTCCGCTACCGCATCTTCGA
CCAGCAGGGCGTGGTTGCCGAGGCGCTACCGGTCACGCGCGGGCTGTACTTCGGCTTCACCCAGACCTCGGTGTGGGACC
TGGAGTCCGATTCCAAGCCCTTCCGTGACACCAGCTTCCGCCCCTCGCTGTTCTACCAGTGGAAGCTGCTCGACCCGAGC
GATGGCGCCTCGCTGACGCTCGCCGGCGGCTACGAGCATGAATCGAACGGCCGCAGCGGACTGGAGTCGCGCAGCATCGA
CACCTTCTTCGTGCGCGGCGACGCGCGCTACTACTTCGGCGACGGCCAAACCTACTTCGCGGTGGCGCCCAAGGCGTGGC
ACTACATCGACAAGGACGACAACCCGGACATCACACGCTACCGCGGTTACGCGGAACTCGGCCTGCGCTTCGGGCGCGAC
GACGGCCTGCTGCTCGCCGCCCAGCTGCGCCGCGGCACCGCCGGAGTCGGCAGCACGCAGCTCGATCTCTCCTATCCGCT
GCGCCGCAGCGTGTTCTCGGGCGTCGGCGCCTTCCTGCACCTGCAGTACTTCAACGGCTATGGCGAAACCCTGCTGGACT
ACAACGAGGCGCGTTCGCCGCAGATTAGAATCGGCCTTTCCCTCGTCAGATGA

Upstream 100 bases:

>100_bases
GCAAGGCGCCCTTCTTGTTTGAGCCATCGAAAACTTTGCTGACGCCCCGGCCGGGCGCTGCTCGCTAGAATCGCGCTGTT
GTCCGTTCGGGGAGTCCGAA

Downstream 100 bases:

>100_bases
GCGCCGCGGGCGCAAGGAGATCCCATGACCCATGCCATCCGCTTCCACCAGACGGGCGGTCCCGAAGTGCTGCAGTGGGA
GGAGGTCGAGCCGGGTGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSLRPCLSLLCLLVGGAAVAAPAAAPDAWLLASPEPRVVPGQPFEVIVIAPSALGEWPATLPAQIELAAPGPRIAIDLVP
ANVPAQGGQRRYIGHWPREVVGVATLSLREAASARLLMDAAAASRSPVEVARSVPVDAAADVAAQAPGAEPAQPAALGFH
EPMYFVAGGRNPRSARYQLSFRYRIFDQQGVVAEALPVTRGLYFGFTQTSVWDLESDSKPFRDTSFRPSLFYQWKLLDPS
DGASLTLAGGYEHESNGRSGLESRSIDTFFVRGDARYYFGDGQTYFAVAPKAWHYIDKDDNPDITRYRGYAELGLRFGRD
DGLLLAAQLRRGTAGVGSTQLDLSYPLRRSVFSGVGAFLHLQYFNGYGETLLDYNEARSPQIRIGLSLVR

Sequences:

>Translated_390_residues
MSLRPCLSLLCLLVGGAAVAAPAAAPDAWLLASPEPRVVPGQPFEVIVIAPSALGEWPATLPAQIELAAPGPRIAIDLVP
ANVPAQGGQRRYIGHWPREVVGVATLSLREAASARLLMDAAAASRSPVEVARSVPVDAAADVAAQAPGAEPAQPAALGFH
EPMYFVAGGRNPRSARYQLSFRYRIFDQQGVVAEALPVTRGLYFGFTQTSVWDLESDSKPFRDTSFRPSLFYQWKLLDPS
DGASLTLAGGYEHESNGRSGLESRSIDTFFVRGDARYYFGDGQTYFAVAPKAWHYIDKDDNPDITRYRGYAELGLRFGRD
DGLLLAAQLRRGTAGVGSTQLDLSYPLRRSVFSGVGAFLHLQYFNGYGETLLDYNEARSPQIRIGLSLVR
>Mature_389_residues
SLRPCLSLLCLLVGGAAVAAPAAAPDAWLLASPEPRVVPGQPFEVIVIAPSALGEWPATLPAQIELAAPGPRIAIDLVPA
NVPAQGGQRRYIGHWPREVVGVATLSLREAASARLLMDAAAASRSPVEVARSVPVDAAADVAAQAPGAEPAQPAALGFHE
PMYFVAGGRNPRSARYQLSFRYRIFDQQGVVAEALPVTRGLYFGFTQTSVWDLESDSKPFRDTSFRPSLFYQWKLLDPSD
GASLTLAGGYEHESNGRSGLESRSIDTFFVRGDARYYFGDGQTYFAVAPKAWHYIDKDDNPDITRYRGYAELGLRFGRDD
GLLLAAQLRRGTAGVGSTQLDLSYPLRRSVFSGVGAFLHLQYFNGYGETLLDYNEARSPQIRIGLSLVR

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=206, Percent_Identity=34.4660194174757, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 42247; Mature: 42116

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRPCLSLLCLLVGGAAVAAPAAAPDAWLLASPEPRVVPGQPFEVIVIAPSALGEWPAT
CCHHHHHHHHHHHHCCHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCC
LPAQIELAAPGPRIAIDLVPANVPAQGGQRRYIGHWPREVVGVATLSLREAASARLLMDA
CCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAASRSPVEVARSVPVDAAADVAAQAPGAEPAQPAALGFHEPMYFVAGGRNPRSARYQLS
HHCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
FRYRIFDQQGVVAEALPVTRGLYFGFTQTSVWDLESDSKPFRDTSFRPSLFYQWKLLDPS
EEEEEECCCCCCHHHHHHHCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCC
DGASLTLAGGYEHESNGRSGLESRSIDTFFVRGDARYYFGDGQTYFAVAPKAWHYIDKDD
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCEEECCCCC
NPDITRYRGYAELGLRFGRDDGLLLAAQLRRGTAGVGSTQLDLSYPLRRSVFSGVGAFLH
CCCHHHCCCHHHHCEEECCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCEEEE
LQYFNGYGETLLDYNEARSPQIRIGLSLVR
EEEECCCCCHHHCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
SLRPCLSLLCLLVGGAAVAAPAAAPDAWLLASPEPRVVPGQPFEVIVIAPSALGEWPAT
CHHHHHHHHHHHHCCHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCC
LPAQIELAAPGPRIAIDLVPANVPAQGGQRRYIGHWPREVVGVATLSLREAASARLLMDA
CCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAASRSPVEVARSVPVDAAADVAAQAPGAEPAQPAALGFHEPMYFVAGGRNPRSARYQLS
HHCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
FRYRIFDQQGVVAEALPVTRGLYFGFTQTSVWDLESDSKPFRDTSFRPSLFYQWKLLDPS
EEEEEECCCCCCHHHHHHHCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCC
DGASLTLAGGYEHESNGRSGLESRSIDTFFVRGDARYYFGDGQTYFAVAPKAWHYIDKDD
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCEEECCCCC
NPDITRYRGYAELGLRFGRDDGLLLAAQLRRGTAGVGSTQLDLSYPLRRSVFSGVGAFLH
CCCHHHCCCHHHHCEEECCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCEEEE
LQYFNGYGETLLDYNEARSPQIRIGLSLVR
EEEECCCCCHHHCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]