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The map label for this gene is leuA1 [H]

Identifier: 119899452

GI number: 119899452

Start: 3470652

End: 3472187

Strand: Direct

Name: leuA1 [H]

Synonym: azo3162

Alternate gene names: 119899452

Gene position: 3470652-3472187 (Clockwise)

Preceding gene: 119899451

Following gene: 119899455

Centisome position: 79.31

GC content: 65.62

Gene sequence:

>1536_bases
ATGAAAGACGCATTGATCATCTTCGATACCACCTTGCGCGACGGCGAACAGAGCCCGGGCGCCTCGATGACCCGCGACGA
GAAGCTGCGCATCGCGCGCCAGCTCGAACGCATGCGGGTCGACGTGATCGAGGCCGGCTTTGCGGCGGCCTCCAACGGCG
ACTTCGAGTCGGTGCGCTCGATCGCCGAGGCGATCAAGGAATCCACCGTCTGTTCGTTGGCGCGCGCCAACGAGAACGAC
ATCCGCCGCGCCGGCGAGGCGATCCGCCCCGCCGCCCGCGGCCGCATCCACACCTTCATCGCGACCAGCCCGATCCACAT
GGAGAAGAAGCTGCGGATGACGCCGGACCAGGTGGTGGAACAGGCGGTGAAGGCGATCGGCTGGGCGCGCGAATACACCG
ATGACATCGAGTTCTCGGCCGAGGACGCGGGCCGCTCCGAGATCGATTTCCTGTGCCGCATCTTCGACGCGGTGATCAAG
GCCGGTGCAAAGACGATCAACGTACCCGACACCGTAGGCTACAACGTCCCGGAGCAGTACGCCGCGACCCTGCGCACCCT
GATCGAGCGCGTACCGAACGCGGACAAGGTGATCTGGTCGGTGCACTGCCACAACGACCTCGGCCTCGCGGTATCGAATT
CCCTCGCCGCCGTGATGGCGGGCGCGCGTCAGGTGGAATGCACGATCAACGGCCTGGGCGAACGCGCCGGCAACGCGTCG
CTCGAAGAGATCGTCATGGCGGTGCGTACCCGGGCCGACGTGTTCCCGGTCAGCACCAACATCGACACCACGCAGATCGT
CCCGGCATCCAAGCTGGTGTCGCAGATCACCGGCTACCCGGTGCAGCCGAACAAGGCCATCGTCGGCGCCAACGCCTTCG
CGCACGAATCCGGCATCCACCAGGATGGCGTGCTCAAGCATCGTGAAACCTACGAGATCATGCGCGCCGAAGACGTGGGC
TGGGGCGCGAACAAGCTCGTGCTCGGCAAGCACTCGGGGCGCAACGCGTTCCGCGCGCGCCTGCAGGAACTGGGTATCGA
GATCGGCTCCGAAGAACAGCTCAACCATGCCTTCGCCCGCTTCAAGGAACTTGCCGACAAGAAGCACGAGATCTTCGACG
AGGATCTCCACGCGCTGATGAGCGATGAGGCGGTGACGCCCGAGCAGGAGCATTTCCGTCTGGTGGCCACCCGCTTCCAT
TCGGAAACCGGCGAAACGCCGCGCGCCGAAGTCACGCTCGCGATCGGCGGCCAGGAAACGCGCAGCCAGGCGGAAGGCTC
CGGACCGGTCGATGCGGCGTTCAAGGCGATCGAGTCGGTGGCCGGCAGCGGCAGCGAACTGCTGCTCTATTCGGTCAACG
CGATCACCACCGGCACCGATGCGCAGGGCGAGGTGACCGTGCGCCTCGCCAAGGACGGCCGCGTCGTGAATGGCCAGGGC
GCCGACACCGACATCATCGTCGCCTCGGCGAAGGCCTACCTCAACGCGCTCAACAAGCTCTGCGGCGGCTCGGAAAAACT
CAACCCGCAAGTCTGA

Upstream 100 bases:

>100_bases
CGCTAATACTCATACCCCTCTTCGTGAAGCCTTCCCCGCAGCCGACGCACCCCGCCCGGGGCGCTCGCGCGCTGCCACCC
CGAATGCCCAGGAGCCTCCC

Downstream 100 bases:

>100_bases
ACCAGCACCACGCGCACGAAAGAAGGGGGCAGACGCCAACGGGCGCCTGCCCCCTTCTCCATTTGCCGCAAGTGATCAGC
GCGGCTGCCGCGACGGCGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKDALIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIEAGFAAASNGDFESVRSIAEAIKESTVCSLARANEND
IRRAGEAIRPAARGRIHTFIATSPIHMEKKLRMTPDQVVEQAVKAIGWAREYTDDIEFSAEDAGRSEIDFLCRIFDAVIK
AGAKTINVPDTVGYNVPEQYAATLRTLIERVPNADKVIWSVHCHNDLGLAVSNSLAAVMAGARQVECTINGLGERAGNAS
LEEIVMAVRTRADVFPVSTNIDTTQIVPASKLVSQITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLVLGKHSGRNAFRARLQELGIEIGSEEQLNHAFARFKELADKKHEIFDEDLHALMSDEAVTPEQEHFRLVATRFH
SETGETPRAEVTLAIGGQETRSQAEGSGPVDAAFKAIESVAGSGSELLLYSVNAITTGTDAQGEVTVRLAKDGRVVNGQG
ADTDIIVASAKAYLNALNKLCGGSEKLNPQV

Sequences:

>Translated_511_residues
MKDALIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIEAGFAAASNGDFESVRSIAEAIKESTVCSLARANEND
IRRAGEAIRPAARGRIHTFIATSPIHMEKKLRMTPDQVVEQAVKAIGWAREYTDDIEFSAEDAGRSEIDFLCRIFDAVIK
AGAKTINVPDTVGYNVPEQYAATLRTLIERVPNADKVIWSVHCHNDLGLAVSNSLAAVMAGARQVECTINGLGERAGNAS
LEEIVMAVRTRADVFPVSTNIDTTQIVPASKLVSQITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLVLGKHSGRNAFRARLQELGIEIGSEEQLNHAFARFKELADKKHEIFDEDLHALMSDEAVTPEQEHFRLVATRFH
SETGETPRAEVTLAIGGQETRSQAEGSGPVDAAFKAIESVAGSGSELLLYSVNAITTGTDAQGEVTVRLAKDGRVVNGQG
ADTDIIVASAKAYLNALNKLCGGSEKLNPQV
>Mature_511_residues
MKDALIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIEAGFAAASNGDFESVRSIAEAIKESTVCSLARANEND
IRRAGEAIRPAARGRIHTFIATSPIHMEKKLRMTPDQVVEQAVKAIGWAREYTDDIEFSAEDAGRSEIDFLCRIFDAVIK
AGAKTINVPDTVGYNVPEQYAATLRTLIERVPNADKVIWSVHCHNDLGLAVSNSLAAVMAGARQVECTINGLGERAGNAS
LEEIVMAVRTRADVFPVSTNIDTTQIVPASKLVSQITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLVLGKHSGRNAFRARLQELGIEIGSEEQLNHAFARFKELADKKHEIFDEDLHALMSDEAVTPEQEHFRLVATRFH
SETGETPRAEVTLAIGGQETRSQAEGSGPVDAAFKAIESVAGSGSELLLYSVNAITTGTDAQGEVTVRLAKDGRVVNGQG
ADTDIIVASAKAYLNALNKLCGGSEKLNPQV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=51.4851485148515, Blast_Score=485, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=25, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=27.906976744186, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=536, Percent_Identity=25.1865671641791, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=28.6111111111111, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55353; Mature: 55353

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDALIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIEAGFAAASNGDFESVRS
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAEAIKESTVCSLARANENDIRRAGEAIRPAARGRIHTFIATSPIHMEKKLRMTPDQVVE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCEEEEEECCCCCHHHHCCCCHHHHHH
QAVKAIGWAREYTDDIEFSAEDAGRSEIDFLCRIFDAVIKAGAKTINVPDTVGYNVPEQY
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHH
AATLRTLIERVPNADKVIWSVHCHNDLGLAVSNSLAAVMAGARQVECTINGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIVMAVRTRADVFPVSTNIDTTQIVPASKLVSQITGYPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLVLGKHSGRNAFRARLQELGIEIGSEEQLNHAFAR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
FKELADKKHEIFDEDLHALMSDEAVTPEQEHFRLVATRFHSETGETPRAEVTLAIGGQET
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHH
RSQAEGSGPVDAAFKAIESVAGSGSELLLYSVNAITTGTDAQGEVTVRLAKDGRVVNGQG
HHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCEEECCCC
ADTDIIVASAKAYLNALNKLCGGSEKLNPQV
CCCEEEEEHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKDALIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIEAGFAAASNGDFESVRS
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAEAIKESTVCSLARANENDIRRAGEAIRPAARGRIHTFIATSPIHMEKKLRMTPDQVVE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCEEEEEECCCCCHHHHCCCCHHHHHH
QAVKAIGWAREYTDDIEFSAEDAGRSEIDFLCRIFDAVIKAGAKTINVPDTVGYNVPEQY
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHH
AATLRTLIERVPNADKVIWSVHCHNDLGLAVSNSLAAVMAGARQVECTINGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIVMAVRTRADVFPVSTNIDTTQIVPASKLVSQITGYPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLVLGKHSGRNAFRARLQELGIEIGSEEQLNHAFAR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
FKELADKKHEIFDEDLHALMSDEAVTPEQEHFRLVATRFHSETGETPRAEVTLAIGGQET
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHH
RSQAEGSGPVDAAFKAIESVAGSGSELLLYSVNAITTGTDAQGEVTVRLAKDGRVVNGQG
HHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCEEECCCC
ADTDIIVASAKAYLNALNKLCGGSEKLNPQV
CCCEEEEEHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA