Click here to switch to the map view.

The map label for this gene is boxA [H]

Identifier: 119899348

GI number: 119899348

Start: 3363690

End: 3364940

Strand: Direct

Name: boxA [H]

Synonym: azo3058

Alternate gene names: 119899348

Gene position: 3363690-3364940 (Clockwise)

Preceding gene: 119899347

Following gene: 119899349

Centisome position: 76.87

GC content: 65.95

Gene sequence:

>1251_bases
ATGAACGCGCCAGCAGAGCACGCCAACATCGCCCGTCAGCACCTGATCGATCCGGAGATCTGCATCCGCTGCAACACCTG
CGAGGAGATCTGCCCGGTCGACGCCATTACCCACGACAACCTCAACTACGTCGTCAAGTTCGACGTCTGCAACGGTTGCC
TGGCCTGCATCTCGCCCTGTCCCACCGGCGCGATCGACTCCTGGCGCAACGTGGAAAAAGCCGTTCCCTACACGCTGGAA
GAACAATTCACCTGGGACGTGCTGCCCGACACCCGCGAACTCGACCAGTTCGAGGCGGGCATCGTCAGCGGCCCGGCCGG
AGAAACCCCGGCCGAGGTGCAGCAGATCACCGACGTCGCCACCGCCGGCCATGGCGGCCCGGCGCTCGCGCCGTGGTCGG
CCTCGCACCCCTACATCAACCTGTACGGCCCCACCAACCCGGTCACCGCCACCGTCACCGGCAACTACCGGCTGACCGAC
GCGGATGCGTCCTCCGACATCCACCACGTGGTGCTGGATTTCGGCAGCACGCCTTTCCCGGTGCTCGAAGGCCAGTCGAT
CGGCATCATCCCGCCGGGCGTGGATGAGAAGGGCAAGCCGCACCTGCTGCGCATGTATTCGGTGGCGAGCCCGCGCGAAG
GCGAGCGCCCGCACCACAACAACCTGTCGCTCACGGTCAAGCGCGTCACCGAAGACCACGAAGGCAAGCCGGCCCGCGGC
GTCGCCTCCAACTACGTGTGCGACCTCAAGAAGGGCGACAAGGTGCAGGTCACCGGCCCCTACGGTTCGACCTTCCTGAT
GCCGAACAATCCGGGTTCGTCGATCATGATGATCTGCACCGGCACCGGCTCGGCGCCGATGCGCGCGATGACCGAACGGC
GCCGCCGCCGCATGGAGCTGAAGGAAGGCGGCGACCTGATCCTGTTCTTCGGCGCCCGCTCGCCCGGCGAACTGCCCTAC
TTCGGCCCGCTGCAGAAGCTGCCGAAGGACTTCATCGACATCAACTTCGCCTTCTCGCGCGTGCCCGGCGAACCCAAGAT
GTACGTGCAGGACCGCATCCGCGAACGCGCCGACAAGGTGTTCGCGATGCTGATGGACGACAACGCCTACATCTACATCT
GCGGCCTCAAGGGCATGGAAGCCGGCGTCGTCGAAGCCTTCCGCGACATCTGCCGCGCCCGCGGCGCGGACTGGGACACG
CTGCGGCCGCAGCTGCTGGCGAAGGGCCGGTTCCATATCGAGACGTATTGA

Upstream 100 bases:

>100_bases
CAGGCGCGACGAGAACGCCAGACGAACCCCATCCCCACCCCAGCCCTCCCCTTGAAGGGGAGGGAGTTCCGCCGCCCAGC
CCACGGAGAAAGGAGACACC

Downstream 100 bases:

>100_bases
GGGGGTTGCTGGAGCAGGGTGACAGGAGGCGCGCTTTATCCCCTCCCCTTCAAGGGGAGGGCTAGGGTGGGGATGGGGTC
AGAGGCTGATCGAGCACTGA

Product: putative benzoyl-CoA oxygenase component A

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNAPAEHANIARQHLIDPEICIRCNTCEEICPVDAITHDNLNYVVKFDVCNGCLACISPCPTGAIDSWRNVEKAVPYTLE
EQFTWDVLPDTRELDQFEAGIVSGPAGETPAEVQQITDVATAGHGGPALAPWSASHPYINLYGPTNPVTATVTGNYRLTD
ADASSDIHHVVLDFGSTPFPVLEGQSIGIIPPGVDEKGKPHLLRMYSVASPREGERPHHNNLSLTVKRVTEDHEGKPARG
VASNYVCDLKKGDKVQVTGPYGSTFLMPNNPGSSIMMICTGTGSAPMRAMTERRRRRMELKEGGDLILFFGARSPGELPY
FGPLQKLPKDFIDINFAFSRVPGEPKMYVQDRIRERADKVFAMLMDDNAYIYICGLKGMEAGVVEAFRDICRARGADWDT
LRPQLLAKGRFHIETY

Sequences:

>Translated_416_residues
MNAPAEHANIARQHLIDPEICIRCNTCEEICPVDAITHDNLNYVVKFDVCNGCLACISPCPTGAIDSWRNVEKAVPYTLE
EQFTWDVLPDTRELDQFEAGIVSGPAGETPAEVQQITDVATAGHGGPALAPWSASHPYINLYGPTNPVTATVTGNYRLTD
ADASSDIHHVVLDFGSTPFPVLEGQSIGIIPPGVDEKGKPHLLRMYSVASPREGERPHHNNLSLTVKRVTEDHEGKPARG
VASNYVCDLKKGDKVQVTGPYGSTFLMPNNPGSSIMMICTGTGSAPMRAMTERRRRRMELKEGGDLILFFGARSPGELPY
FGPLQKLPKDFIDINFAFSRVPGEPKMYVQDRIRERADKVFAMLMDDNAYIYICGLKGMEAGVVEAFRDICRARGADWDT
LRPQLLAKGRFHIETY
>Mature_416_residues
MNAPAEHANIARQHLIDPEICIRCNTCEEICPVDAITHDNLNYVVKFDVCNGCLACISPCPTGAIDSWRNVEKAVPYTLE
EQFTWDVLPDTRELDQFEAGIVSGPAGETPAEVQQITDVATAGHGGPALAPWSASHPYINLYGPTNPVTATVTGNYRLTD
ADASSDIHHVVLDFGSTPFPVLEGQSIGIIPPGVDEKGKPHLLRMYSVASPREGERPHHNNLSLTVKRVTEDHEGKPARG
VASNYVCDLKKGDKVQVTGPYGSTFLMPNNPGSSIMMICTGTGSAPMRAMTERRRRRMELKEGGDLILFFGARSPGELPY
FGPLQKLPKDFIDINFAFSRVPGEPKMYVQDRIRERADKVFAMLMDDNAYIYICGLKGMEAGVVEAFRDICRARGADWDT
LRPQLLAKGRFHIETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=198, Percent_Identity=31.3131313131313, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI10835173, Length=201, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI40254422, Length=201, Percent_Identity=30.8457711442786, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789123, Length=312, Percent_Identity=24.6794871794872, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554134, Length=198, Percent_Identity=29.7979797979798, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321143, Length=225, Percent_Identity=30.2222222222222, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=170, Percent_Identity=33.5294117647059, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583543, Length=223, Percent_Identity=28.6995515695067, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI78706876, Length=223, Percent_Identity=28.6995515695067, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI78706872, Length=223, Percent_Identity=28.6995515695067, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24582192, Length=200, Percent_Identity=30.5, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137192, Length=200, Percent_Identity=30.5, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 45820; Mature: 45820

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPAEHANIARQHLIDPEICIRCNTCEEICPVDAITHDNLNYVVKFDVCNGCLACISPC
CCCCHHHHHHHHHHCCCHHHEEEECCHHHHCCCCEEEECCCEEEEEEECCCCHHHHHCCC
PTGAIDSWRNVEKAVPYTLEEQFTWDVLPDTRELDQFEAGIVSGPAGETPAEVQQITDVA
CCCCCHHHHHHHHHCCEEECCCEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TAGHGGPALAPWSASHPYINLYGPTNPVTATVTGNYRLTDADASSDIHHVVLDFGSTPFP
HCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCEEEEEECCCCCCC
VLEGQSIGIIPPGVDEKGKPHLLRMYSVASPREGERPHHNNLSLTVKRVTEDHEGKPARG
EECCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
VASNYVCDLKKGDKVQVTGPYGSTFLMPNNPGSSIMMICTGTGSAPMRAMTERRRRRMEL
CCCCEEEECCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KEGGDLILFFGARSPGELPYFGPLQKLPKDFIDINFAFSRVPGEPKMYVQDRIRERADKV
HCCCCEEEEEECCCCCCCCCCCCHHHCCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHE
FAMLMDDNAYIYICGLKGMEAGVVEAFRDICRARGADWDTLRPQLLAKGRFHIETY
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHCCHHHHHCCEEEEECC
>Mature Secondary Structure
MNAPAEHANIARQHLIDPEICIRCNTCEEICPVDAITHDNLNYVVKFDVCNGCLACISPC
CCCCHHHHHHHHHHCCCHHHEEEECCHHHHCCCCEEEECCCEEEEEEECCCCHHHHHCCC
PTGAIDSWRNVEKAVPYTLEEQFTWDVLPDTRELDQFEAGIVSGPAGETPAEVQQITDVA
CCCCCHHHHHHHHHCCEEECCCEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TAGHGGPALAPWSASHPYINLYGPTNPVTATVTGNYRLTDADASSDIHHVVLDFGSTPFP
HCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCEEEEEECCCCCCC
VLEGQSIGIIPPGVDEKGKPHLLRMYSVASPREGERPHHNNLSLTVKRVTEDHEGKPARG
EECCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
VASNYVCDLKKGDKVQVTGPYGSTFLMPNNPGSSIMMICTGTGSAPMRAMTERRRRRMEL
CCCCEEEECCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KEGGDLILFFGARSPGELPYFGPLQKLPKDFIDINFAFSRVPGEPKMYVQDRIRERADKV
HCCCCEEEEEECCCCCCCCCCCCHHHCCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHE
FAMLMDDNAYIYICGLKGMEAGVVEAFRDICRARGADWDTLRPQLLAKGRFHIETY
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHCCHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]